• Journal of the Korean Society of Clothing and Textiles
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• v.20 no.2
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• pp.390-397
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• 1996

The effects of the mixture of surfactants on the removal of triolein applied to cotton and polyester fabrics were studied. The sunactants which were usually formulated to the Iriundry detergents, such as sodium dodecyl sulfonate (LAS), sodium lauryl sulfate (AS), $\alpha$-olefin sulfonate (AOS, C=14), sodium laurethoxy sulfate (AES, EO=3) and lauryl ethoxylate (AE, EO=7) were used. Washing was done at 40'C, 60"C, 80'c and the surfactant concentration was 0.05%. Backscattered electron imaging was made to study the location of triolein in the yarns. Triolein was removed easily from polyester than cotton fabrics.01eic acid added triolein wa9 removed greater than triolein only, except in AE solutions. In AE solutions, the removal of triolein was increased as the tremperature went higher and the increasing rate of the removal of triolein only was greater than that of oleic acid added triolein. In the result, more triolein was removed from triolein only than from oleic acid added triolein at 80t When the other surfactant was added to LAS, AES was the best to cotton fabrics, AOS was to polyester fabrics. Triolein was located in the lumen and grooves of the fibers and the deeper interfiber spaces. Triolein did not make thin film arround the cotton fiber in the surface, but polyester fibers. These are the main reason why the removal of triolein was difficult from cotton fabrics.rics.

• Journal of the Korean Society of Clothing and Textiles
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• v.25 no.5
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• pp.991-1001
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• 2001

This study has examined effects of protease on the removal of hemoglobin and triolein mixed soil with changes of soil content and soil composition from cotton and PET filament fabrics. The results obtained were as follows: The results obtained were as follows: 1) The detergency of PET fabrics was higher than that of cotton fabrics. The removal of hemoglobin was much higher than that of triolein from cotton fabrics, while the removal of hemoglobin was similar to that of triolein from PET fabrics. 2) The removal efficiency of hemoglobin and triolein was improved by protease from cotton and PET fabrics. Especially the removal efficiency of those was remarkably improved from cotton fabrics which the removal of soil was lower than PET fabrics. And the removal of hemoglobin and triolein by adding protease was increased with increase of hemoglobin content of mixed soil from cotton fabrics. 3) With the increase of hemoglobin content of mixed soil, the removal of hemoglobin was drastically increased but the removal of triolein was slightly decreased from cotton and PET fabrics. With the increase of triolein content of mixed soil, the removal of hemoglobin and triolein was decreased from cotton fabrics, but those were generally increased from PET fabrics.

• Journal of the Korean Society of Clothing and Textiles
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• v.19 no.2
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• pp.356-365
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• 1995

study was made to investigate the emulsification and rolling-up between liquid oily soils and surfactant and its effect on the detergency. Samples used were triolein as a triglyceride, oleic acid as a free fatty acid and sodium dodecyl sUJfate(SDS) as a surfactant. The results were as follows: 1. The spontaneous emulsification occurred in the system of oleic acid alone and mixture of triolein and oleic acid contacted with 0.5% SDS solution, but it did not occurred in a case of triolein alone. 2. The stability of emulsification increased with the increase of SDS concentration. And the stability of emulsification and (-) t value increased in the order of triolein < mixture of triolein and oleic and< oleic acid. 3. The rolling-up mechanism of model oily soils easily occurred in the order of triolein< mixture of triolein and oleic acid< oleic acid, and facilitated with the increase of SDS concentration. On the other hand, the complex formation was already observed in the system of oleic and 0.5% SDS solution. 4. As compared with the detergency of triolein, the detergency of oleic acid was very high. And the detergency of mixed soil was improved with increasing ratio of oleic acid in the mixture.

• Applied Biological Chemistry
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• v.23 no.1
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• pp.52-57
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• 1980

This study was conducted to clarify the mode of action and the positional specificity of Trichosporon cutaneum lipase during the course of hydrolysis of triolein and monoolein mixture by thin-layer chromatography. 1. The hydrolytic activity of the lipase to oleyl glycerides was in the order of triolein>diolein>monoolein. 2. Both of triolein and diolein were hydrolyzed by the lipase at high and almost the same rate. 3. The hydrolysis of monoolein by the lipase was very slow compared to the other two oleyl glycerides. 4. This lipase appeared to have a very low specificity toward the outer chains of triolein.

• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.2
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• pp.103-108
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• 1998

A hydrophobic substrate triolein was hydrolyzed by lipase in a mono-phase reaction system containing cyclodextrin(CD) as emulsifier. The triolein was transformation to an emulsion-like state in the CD containing reaction system in contrast to the oil-droplet like state without CD due to the formation of an inclusion complex between the lipids and CDs. The hydyrolysis reaction increased substantially in the CD containing reaction system, and the optimum reaction conditions including the amount of lipase, ${\beta}$-CD concentration, and mixing ratio of triolein and ${\beta}$-CD, were determined. The performance of the enzyme reaction in a mono-phase reaction system was compared with that of a two-phase reaction system which used water immiscible hexane as the organic solvent. The role of a CD in the mono-phase reaction system was elucidated by comparing the degree of the inclusion complex formation with triolein and oleic acid, Km and Vmax values, and product inhibition by oleic aicd in aqueous and CD containing reaction systems. The resulting enhanced reaction seems to be caused by two phenomena; the increased accessibility of lipase to triolein and reduced product inhibition by oleic acid through the formation of an inclusion complex.

• Journal of the Korean Society of Clothing and Textiles
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• v.16 no.1 s.41
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• pp.65-71
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• 1992

The influences of hydrophilic treatment of the PET fabric on soiling and detergency of triolein were studied. The amounts of residual triolein were determined by radiotracer analysis, and distributions of the unsaturated oils on the fabric were evaluated by backscattered electron images. The removal of triolein was increased when the PET fabrics were treated. SRP pretreatment was more effective on the oily soil removal than the of addition of SRP in the detergent. The oily soil of triolein only was packed between the fibers, but mixed soil was distributed around the fibers. When the mixed soil was used, detergent solution could penetrate the continuous interfiber capillaries, this would be one of the reasons that mixed soil was removed rmore extensively.

• Textile Coloration and Finishing
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• v.13 no.2
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• pp.18-18
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• 2001

This study has examined the removal of mixed soil of protein and fat by protease. Cotton and PET fabrics were soiled by spotting of hemoglobin and triolein, respectively. The soiling order and soil concentration were changed in this procedure. The soiled fabrics were aged at 130℃ for 20 minutes. Protease was added in the alcohol ethoxylate(AE) detergent solution. The removal effciency was evaluated by analysis of protein and/or fat on the fabrics before and after washing, respectively. The detergency of PET fabrics was higher than that of cotton fabrics. The removal efficiency of hemoglobin was improved by protease from cotton and PET fabrics. Especially the removal efficiency of hemoglobin was remarkably improved from cotton fabrics. With the increase of hemoglobin and triolein (1:1) mixed soil, the removal of mixed soil was increased in proportion to mixed soil content up to a certain point. but it began to decrease above the point from cotton fabrics, while it was generally increased from PET fabrics. The detergency of total mixed soil from cotton fabrics was higher in case of soiling order with triolein after hemoglobin than in case of soiling order with triolein before hemoglobin. But the soiling order was not greatly effected in the detergency of total mixed soil from PET fabrics.

• Textile Coloration and Finishing
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• v.13 no.2
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• pp.104-113
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• 2001

This study has examined the removal of mixed soil of protein and fat by protease. Cotton and PET fabrics were soiled by spotting of hemoglobin and triolein, respectively. The soiling order and soil concentration were changed in this procedure. The soiled fabrics were aged at $130^\circ{C}$ for 20 minutes. Protease was added in the alcohol ethoxylate(AE) detergent solution. The removal effciency was evaluated by analysis of protein and/or fat on the fabrics before and after washing, respectively. The detergency of PET fabrics was higher than that of cotton fabrics. The removal efficiency of hemoglobin was improved by protease from cotton and PET fabrics. Especially the removal efficiency of hemoglobin was remarkably improved from cotton fabrics. With the increase of hemoglobin and triolein (1:1) mixed soil, the removal of mixed soil was increased in proportion to mixed soil content up to a certain point. but it began to decrease above the point from cotton fabrics, while it was generally increased from PET fabrics. The detergency of total mixed soil from cotton fabrics was higher in case of soiling order with triolein after hemoglobin than in case of soiling order with triolein before hemoglobin. But the soiling order was not greatly effected in the detergency of total mixed soil from PET fabrics.

• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.480-490
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• 2020

A novel lipase gene, Lip-1420, was isolated from a metagenomic library constructed from reed marsh from Mt. Jumbong in Korea, comprising 112,500 members of recombinant plasmids. The DNA sequence of Lip-1420-subclone (5,513 bp) was found to contain at least 11 ORFs according to the GenBank database. The ORF-3 gene was inserted into the pET21a plasmid containing the C-terminal 6-His tag and transformed into E. coli BL21(DE3) to express the recombinant lipase protein. Lip-1420 was purified using a fast protein liquid chromatography system. The gene was registered in GenBank (MH628529). The values of Km and Vmax were determined as 0.268 mM and 1.821 units, respectively, at 40℃ and pH 8.0, using p-nitrophenyl palmitate as the substrate. This lipase belongs to family IV taxonomically because it has conserved HGGG and GDSAG motifs in the constitutive amino acid sequence. According to the predicted structural model, the binding sites are represented by residues H78, G81, D150, S151, A152, V181, and D236. Finally, Lip-1420 showed triolein selectivity for methanolysis between triolein (18:1) and tristearin (18:0) substrates. Further study of the selective mechanism and structure-function relationship of this new lipase could be useful for more practical applications.

• Biomedical Science Letters
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• v.11 no.4
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• pp.555-559
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• 2005

Evaluate the triolein emulsion could disrupt the barriers and to suggest as an experimental model in blood-ocular barrier studies. Triolein emulsion was infused into the carotid artery in the experimental group ten cats. Normal saline was used in another the control group ten cats. Pre contrast and postcontrast T1-weighted MR images were obtained at 30 minutes and 3 hours after embolization. Signal intensities were evaluated in the anterior, posterior chamber and in the vitreus qualitatively and quantitatively. Postembolization 30 minutes MR images were not different from those of the control group. Postembolization 3 hour MR images demonstrated delayed contrast enhancement in the anterior chamber of the ipsilateral and contralateral eyeballs and in the posterior chamber of the ipsilateral eyeball. Delayed contrast enhancement of the posterior chamber of the ipsilateral eyeball was statistically significant (P<0.05). The present study demonstrated significant contrast enhancement in the posterior chamber with infusion of triolein emulsion and can be a model in blood-aqueous barrier studies.