• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.169.1-169
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• 1999

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.169.2-169
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• 1999

No Abstract, See Full Text

• Applied Microscopy
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• v.43 no.3
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• pp.110-116
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• 2013

Effects of elevated air temperature and carbon dioxide ($CO_2$) concentration on the leaf surface structures were investigated in Liriodendron tulipifera (yellow poplar) and Populus tomentiglandulosa (Suwon poplar). Cuttings of the two tree species were exposed to elevated air temperatures at $27/22^{\circ}C$ (day/night) and $CO_2$ concentrations at 770/790 ppm for three months. The abaxial leaf surface of yellow poplar under an ambient condition ($22/17^{\circ}C$ and 380/400 ppm) had stomata and epicuticular waxes (transversely ridged rodlets). A prominent increase in the density of epicuticular waxes was found on the leaves under the elevated condition. Meanwhile, the abaxial leaf surface of Suwon poplar under an ambient condition was covered with long trichomes. The leaves under the elevated condition possessed a higher amount of long trichomes than those under the ambient condition. These results suggest that the two poplar species may change their leaf surface structures under the elevated air temperature and $CO_2$ concentration condition for acclimation of increased photosynthesis.

• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.257-261
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• 2003

This experiment was carried out to investigate the effects of plant growth regulators on the organogenesis from the tissue culture of Arabidopsis thaliana stem, and the origin of the callus development. When the stem segments were cultured on medium with 2mg/L IAA or NAA, adventitious roots and trichomes were differentiated after 11 days of culture. Callus vigorously formed on medium with 2/L2,4 after 7 days of culture, but adventitious roots and trichomes were not differentiated from callus after 10 days of culture. This results suggesting that picloram is very effective auxin for the callus formation and organogenesis. Callus weakly formed on 0.05mg/L kinetin, and formed on combination of auxins(2mg/L) with 0.05mg/L kinetin. But the effect of combination of auxins and kinetin the callus formation was less than 2,4-D or picloram alone. A histological examination of callus formed on picloram showed that phloram showed that phloem parenchyma cells were divided and enlarged after 2 days of culture. Cortex parenchyma cells were divided and meristematic nodules were developed from these cells after 4 days of culture. Finally, callus formed on outside of cortex and epidermis by division of meristematic nodules after 7 days of culture.

• Korean Journal of Plant Taxonomy
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• v.48 no.3
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• pp.172-184
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• 2018

We examined the floral morphology of 15 taxa of five genera (Cynanchum, Marsdenia, Metaplexis, Tylophora, and Vincetoxicum) in Korean Asclepiadoideae using a stereoscopic and scanning electronic microscope to clarify and describe the floral characteristics. In this study, the corolla and corona types, the types of corona lobes, appendages on the corona lobes, and the apex shape of the style head are considered as diagnostic characteristics at the generic level. The genus Vincetoxicum, which is treated as a synonym of the genus Cynanchum in Korea, is distinguished from Cynanchum by the interstaminal part on the corona, fleshy and mainly triangular or ellipsoid corona lobes, and various corolla colors. In Cynanchum, various corona types have been observed, while Vincetoxicum have similar corona types among the taxa. In addition, the main floral characteristics at the species level were as follows: flowering time, inflorescence, corolla color, trichomes on the corolla surfaces, apex shape of the corona lobe, and trichomes on the ovary. This study presents the taxonomic importance of floral morphology by providing descriptions and diagnostic characteristics among the genera and species investigated.

• Journal of Forest and Environmental Science
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• v.35 no.1
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• pp.54-60
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• 2019

Plant tissue culture techniques offer quick methods of regeneration of plants of medicinal importance but the survival chances of such plants are always questionable when shifted to the in vivo conditions. The present study enumerates the micromorphological developments in the leaves of in vitro regenerated and field transferred plantlets of Oldenlandia umbellata. The leaves developed in vitro after $4^{th}$ subcultures of multiplication phase and after 6 weeks of field transferred plants were used. Statistically significant differences in the number of stomata, veins, raphides, crystals and trichome density per square mm were observed. The improvements in stomatal apparatus and density (decreased from 41.85 to 32.20), developments in leaf architectural parameters and emergence of defense mechanism through increased numbers of raphides (8 to 15), crystals and trichomes (13.5 to 18.2) proved acclimation of tissue culture raised plantlets from in vitro to the in vivo environments lead to 100 % success in field establishment of the plantlets. The in vitro induced foliar abnormalities (changes in stomata, venation pattern, vein density, trichomes, crystals etc.) were repaired while hardening of plantlets in the greenhouse and finally in the field. The observed micromorphological response of leaves under altered environmental conditions could help in determination of proper stage of field transfer and prediction of survival percentage of in vitro regenerated O. umbellata plantlets.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.26-26
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• 2020

Daphne L. (Thymelaceae) comprises about 95 species distributing worldwide from N Africa, N India, SE Asia to E Asia and the coast of the Mediterranean of Europe. In Korea, five species of this genus have been described. In this study, we included four species (D. genkwa, D. pseudomezereum, D. kiusiana, D. jejudoensis) from Korea, excluding cultivated D. odora. The morphological characters through local surveys and the re-classification of the specimens collected in the Korea National Herbarium (KH) were carried out and distribution maps for each taxon were also prepared. The major characters include habit, trichomes in winter bud, leaf, and twig, phyllotaxis, inflorescence, size of calyx lobe and trichomes in the calyx tube, etc. The distribution map showed that D. genkwa is mainly distributed in the coastal area of Hwanghaenam-do, Pyeongannam-do, Jeollabuk-do and Jeollanam-do, whereas D. pseudomezereum is distributed in the limestone zone of Gangwon-do, Jeollabuk-do, and Gyeongsangbuk-do. Similarly, D. kiusiana is mostly found in Jeollanam-do, Gyeongsangnam-do, and Jeju-do. In addition, D. jejudoensis is known to be distributed in forests of Murueng, Andeok, and Seonheul-ri in Jeju-do, but recently, new habitat is discovered in the island forest areas of Jeollanam-do. However, some of these individuals showed the characteristics of D. kiusiana, thus before come to any conclusion detailed taxonomic review of D. jejudoensis and D. kiusiana is required.

• Korean Journal of Plant Taxonomy
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• v.37 no.3
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• pp.239-254
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• 2007

A comparative micromorphological study was examined on the leaves of the genus Glechoma and related genera (Nepetinae, Lamiaceae) by scanning electron microscopy (SEM) in order to evaluate their significance in the taxonomy. The leaves of taxa Marmoritis, Nepeta sect. Glechomanthe, G. hederacea var. longituba (Korea) are revealed amphistomatic type, while the remnants of taxa had hypostomatic type. The size range of the guard cells is $12.50-28.75{\times}9.17-21.25{\mu}m$: the smallest one was found in M. pharicus ($12.50-15.83{\times}9.17-11.25{\mu}m$), while the largest one was measured to G. hederacea var. longituba (Korea: $28.75-28.88{\times}21.25-21.38{\mu}m$). The stomatal type of genera Agastache, Dracocephalum was mostly diacytic, however for the rest rarely together with anisocytic and anomocytic, except G. hederaca var. longituba (Korea), Meehania urticifolia by having combined with diacytic and anomocytic. The shapes of epidermal cells are differ from in abaxial and adaxial side, and dived with two types (e.g., platelet, stripe pattern). Five types (three glandular, two non-glandular hairs) of trichomes are distributed in leaves. Among trichomes, long and stalk capitates glandular trichome, subsessile glands are different from studied taxa so that leaf micromorphological characters are significance features in the taxonomy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.205-208
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• 1998

Silica bodies (phytoliths) are becoming of wide use for pedology, archaeology, paleobotany and paleoecology in botany. This study investigated morphological differences of silica bodies in the lamina of wild, indica type, and japonica type rice. Phytoliths in the epidermis of lamina showed noticeable difference among tested plants. Besides, there were also significant differences in the shape and distribution of the silica bodies around stomata and trichomes. Silica bodies in the lamina of the rice plants could be used to classify subspecies of Oryza genus.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.203-203
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• 2005