• Journal of Korean Society of Forest Science
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• v.110 no.2
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• pp.155-164
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• 2021

Many studies have been conducted on developing automatic plant identification algorithms using machine learning to various plant features, such as leaves and flowers. Unlike other plant characteristics, barks show only little change regardless of the season and are maintained for a long period. Nevertheless, barks show a complex shape with a large variation depending on the environment, and there are insufficient materials that can be utilized to train algorithms. Here, in addition to the previously published bark image dataset, BarkNet v.1.0, images of barks were collected, and a dataset consisting of 53 tree species that can be easily observed in Korea was presented. A convolutional neural network (CNN) was trained and tested on the dataset, and the factors that interfere with the model's performance were identified. For CNN architecture, VGG-16 and 19 were utilized. As a result, VGG-16 achieved 90.41% and VGG-19 achieved 92.62% accuracy. When tested on new tree images that do not exist in the original dataset but belong to the same genus or family, it was confirmed that more than 80% of cases were successfully identified as the same genus or family. Meanwhile, it was found that the model tended to misclassify when there were distracting features in the image, including leaves, mosses, and knots. In these cases, we propose that random cropping and classification by majority votes are valid for improving possible errors in training and inferences.

• Korean Journal of Environmental Biology
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• v.40 no.2
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• pp.199-205
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• 2022

Two tree frogs, Dryophytes suweonensis and Dryophytes japonicus, inhabiting Korea, are morphologically similar and share the same habitats. Therefore, they are identified mainly through their calls, especially for males. Dryophytes suweonensis is registered as an endangered (IUCN: EN grade) and protected species in South Korea. Thus, it is necessary to develop a method to rapidly identify and discriminate the two species and establish efficient protection and restoration plans. We identified significant genetic variation between them by sequencing a maternally-inherited mitochondrial 12S ribosomal DNA region. Based on the sequence data, we designed a pair of primers containing 7bp differences for high resolution melting(HRM) analysis to rapidly and accurately characterize their genotypes. The HRM analysis using genomic DNA showed that the melting peak for D. suweonensis was 76.4±0.06℃, whereas that of D. japonicus was 75.0±0.05℃. The differential melt curve plot further showed a distinct difference between them. We also carried out a pilot test for the application of HRM analysis based on immersing D. suweonensis in distilled water for 30 min to generate artificial environmental DNA(eDNA). The results showed 1.10-1.31℃ differences in the melting peaks between the two tree frog samples. Therefore, this HRM analysis is rapid and accurate in identifying two tree frogs not only using their genomic DNA but also using highly non-invasive eDNA.

• Journal of the Korean Wood Science and Technology
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• v.10 no.3
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• pp.190-193
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• 1982

Conclusion: The results of this study are encouraging for the development of a set of interpretable diagnostic criteria for a reasonably reliable identification of some species. The present study has been limited in the characteristics studied and was made on relatively poor photography. Further study on high quality photography, over wider areas and including such additional characteristics as crown texture and shadow intensity should prove useful. A preliminary examination has been made of some photo-image characteristics of a number of important tree species on large scale (1:9,000) contact prints of panchromatic mm. The characteristics studied were crown image tone, and shape. Image tone was estimated against a standard grey-scale and shows within-species consistency and a range between species. Crown shapes were subjectively assessed but there appears to be a within-species consistency and interpretable between-species differences. The results of this trial suggest that it should be practicable to make a number of important species distinctions by photo-interpretation with a useful degree of reliability. Other characteristics beside those studied might be usefully examined. Photography: Hand-printed glossy contact prints of vertical 1:9,000 scale panchromatic photography of Kwangnung Experimental Forest flown May 1964. The filter used is unknown (probably minus-blue). The camera is unknown but was probably a military type K-17 or K-22 with 6"(apostfophy) lens. The photography shows notably poor resolution. Species: 143 individual trees ranging through 11 species (7 softwood, 4 hardwood) were included in the study. A range of size classes were included for each species.

• Journal of Conservation Science
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• v.38 no.2
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• pp.87-95
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• 2022

This aimed to species identification and tree-ring dating of wooden elements used in the Myeongjeong-gate of the Changgyeong-palace, Seoul, Korea. Of the 79 wooden elements evaluated, 78 were confirmed to be hard pines and one belonged to Abies spp.. Cores of the wooden elements were collected using a drill for tree-ring dating, and ring-width plots of individual samples were constructed using the TSAP software. The tree-ring dating results for the outermost ring of 58 hard pine wooden elements revealed the following felling dates: early spring of 1604-late fall of 1615, late fall of 1706-early spring of 1707, and late fall of 1828-late fall of 1834. The obtained felling dates of the 1600s and 1800s were found to be consistent with those in the construction and repair records of the Annals of Joseon Dynasty, Gwanghaegun's Diary and Changgyeonggung Yeonggeondogam Uigwe. However, the obtained felling dates of the 1700s were not consistent with those in the construction and repair records. Therefore, additional confirmation of repairs that were not included in the records was possible using tree-ring dating.

• Journal of Forest and Environmental Science
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• v.34 no.6
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• pp.457-460
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• 2018

Mushroom survey was conducted in the Phou Khao Khuoay National Protected Area (PKKNPA) located at east direction 65km from Vientiane, the capital city of Lao-PDR from September 2015 to November 2016. During this periods, mushroom specimens were collected from 10 different survey sites, and then were identified and classified into 149 species, 113 genera, and 55 families by morphological and molecular analyses. The mushrooms belongs to Ascomycota were classified into 13 species, 7 genera, 5 families, and 5 orders, while those belongs to Basidiomycota were classified into 136 species, 113 genera, 55 families, and 18 orders, respectively. Among these mushrooms, the most species-rich families were Polyporaceae (18.1%), Marasmiaceae (11.0%), Ganodermataceae (8.6%), Xylariaceae (5.8%), Russulaceae (5.4%), Agaricaceae (4.7%), Boletaceae (4.7%), Hymenochaetaceae (3.9%), and Amanitaceae (3.6%), and comprised 65.8% of the total specimens identified.

• Journal of Conservation Science
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• v.36 no.5
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• pp.326-332
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• 2020

The this study's objective was to conduct species identification and tree-ring dating for the wood elements of Daewoong-jeon(main temple), Hwagye-temple, Seoul, Korea. Most of the wood elements were made from Pinus spp.(hard pine). However, one large beam was constructed from Abies spp.. For the tree-ring dating, cores were taken from the elements using a drill, and ring-width plots of individual samples were produced with the TSAP software program. The tree-ring dating results showed that, the date of the outermost ring of most elements (20 elements), including beams and pillars, were from AD 1839 to 1870. Among them, five elements had bark, and felling dates were AD 1868 and 1869. These dates confirmed the historical records that Daewoongjeon was rebuilt in AD 1870 from the records of hidden materials(Bokjangmul) in Jongdori by the survey 2012.

• Journal of the Korean Wood Science and Technology
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• v.49 no.6
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• pp.550-565
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• 2021

This study's objective was to conduct species identification and tree-ring dating of wooden elements used in Mungyeong Juheulgwan of Joryeong (Gate No. 1). Of the 84 wooden elements evaluated, 76 were confirmed to be hard pines, 5 were soft pines, and 3 belonged to Abies spp. For tree-ring dating, cores of the wooden elements were collected using a drill, and ring-width plots of individual samples were constructed using the TSAP software. The results of performing tree-ring dating for the outermost ring of 59 hard pine wooden elements revealed the following 4 felling dates: summer of 1708-late fall of 1709, summer of 1792-early spring of 1794, late fall of 1838-early spring of 1840 and 1867, and early spring-fall of 1872. These felling dates were found to be consistent with those in the construction and repair records of the Annals of Joseon Dynasty, Juhulgwan Jungsugi, and those engraved on Juhulgwan Walls. It is believed that some of the wooden materials harvested at that time were stored and used since there was a difference of approximately10 years between the repair records and felling dates.

• Journal of the Korean Wood Science and Technology
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• v.33 no.2 s.130
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• pp.1-7
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• 2005

Tree species of wooden structural members of the Beomeo Temple were identified based on light and scanning electron microscopic characters in the present study. Of 10 structural members, 9 softwoods and 1 hardwood were identified. Among softwood members, 7 belonged to hard pine of the Sylvestris section, and the remaining 2 to hard pine of other than the Sylvestris section and hemlock of the genus Tsuga, respectively. A single hardwood member was identified as white oak of the Prinus section under the subgenus Lepidobalanus.

• Korean journal of applied entomology
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• v.42 no.4
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• pp.279-288
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• 2003

A total of 29 scale insect species belonging to 21 genera of five families were recognized on 11 fruit trees on the basis of specimens collected from 2001 to 2002 and specimens deposited in the Insect Collection of NIAST in Korea. Ten species were recognized on apple, nine species on pear, three species on peach, 14 species on unshiu orange, nine species on persimmon, two species on Japanese plum, one species on japanese apricot, 10 species on junos orange, one species on grape, four species on jujube and three species on kiwi fruit.

• 한국문화재보존과학회:학술대회논문집
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• 2003.03a
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• pp.27-27
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• 2003