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Radial Variation of Anatomical Characteristics of Chestnut Wood (Castanea crenata) Grown in Korea - Vessel Element and Ray - (국내산 밤나무 목재의 해부학적 특성의 방사방향 변이성 - 도관요소 및 방사조직 -)

  • Lee, Seon-Hwa;Kwon, Sung-Min;Lee, Sung-Jae;Lee, Uk;Kim, Mahn-Jo;Kim, Nam-Hun
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.1
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    • pp.19-28
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    • 2009
  • Some wood anatomical characteristics of seven chestnut cultivars (Tanzawa, Ibuki, Arima, Ginyose, Tsukuba, Riheiguri, and Mansung) grown in Korea were examined in this study. We investigated the radial variation of wood components such as diameter and number of earlywood vessel element and ray density in cross section and number and height of ray in tangential section. Discs were taken from the stem of seven chestnut cultivars at breath height and cut into the radial strip. Cross, radial, and tangential sections, 10 to $20{\mu}m$ thick, were sliced with a sliding microtome. These sections were stained, dehydrated, and mounted with Canada balsam according to common procedure. Measurement and observation were performed with a light microscopy. In all chestnut cultivars, radial and tangential diameter of vessel element increased but number of vessel element per $mm^2$ decreased with increasing tree age. Ray spacing (per mm) in cross section from pith to bark showed no significant variation, while number of ray per $mm^2$ increased but ray height decreased with the increase of tree age. Conclusively, the results obtained in present study were thought to be some valuable information for separating juvenile and adult wood as well as identifying chestnut cultivars.

Impact of Transplanting on Tree Growth and Compartmentalization of Pruning Wounds in Acer palmatum Thunb

  • Lee, Kyu Hwa;Lee, Kyung Joon;Gwak, Ki-Seob;Choi, In-Gyu
    • Journal of Korean Society of Forest Science
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    • v.98 no.5
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    • pp.618-629
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    • 2009
  • The objective of this study was to examine the impact of pruning (P treatment) and transplanting (T treatment) of Acer palmatum on cambial growth and compartmentalization of pruning wounds for one year after treatments. Changes of cambial electrical resistance (CER), sizes of pruning wounds, cambial growth of trunks and stems near the wounds, and total phenols at branch unions during the period were examined using a total of 49 trees. After harvesting, areas of discolored wood behind the wounds, relative proportions of extractives, holocellulose and lignin at branch unions were also determined. CER and the cambial growth of trunk at 30 cm above the ground (TGR) were inversely correlated, and differences of CER and TGR among three treatments were significant. TGRs of control, P treatment and P+T treatment after the treatments were 112.2%, 72.4% and 52.5% of the annual growth for the year before the treatments, respectively. The cambial growth rate of stem (SGR) at 1.5 cm above the branch bark ridge and the closure rate of pruning wound (WCR) for one year after treatments were positively correlated, and WCR of P treatment of 39.8% was significantly higher than that of P+T treatment of 31.8%. Wounds of P+T treatment formed greater discolored area per unit area of pruning wound (D/W Ratio) than those of P treatment significantly. Lower WCR and higher D/W Ratio of P+T treatment suggested less ability of compartmentalizing the wounds than P treatment. Total phenols at branch core of pruning wound for both treatments heightened a month after treatment, and then lowered. The contents at below core of the wound were higher than those at control ones continuously, while they became similar each other at above core. Relatively high phenol contents of the extractives at P+T treatment implied that trees with P+T treatment allocated more energy to compartmentalize their wounds. Holocellulose and lignin contents at the branch core of treated branch unions of both treatments were lower and higher, respectively, than at the same part of the union with living branch, as results of the tree reaction to protection from wounding and microbial invasion.

A Study on the Structure of Biomass Production in Thrifty-Mature Quercus mongolica Stand (신갈나무 장령임분(壯齡林分)의 물질생산(物質生産) 구조(構造)에 관(關)한 연구(硏究))

  • Han, Sang Sup;Kim, Do Young;Sim, Joo Suk
    • Journal of Korean Society of Forest Science
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    • v.81 no.1
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    • pp.1-10
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    • 1992
  • This study was conducted to explain the characters of the structure of biomass production in the thrifty-mature Quercus mongolica stands and investigate the relationships between the leaf weight or leaf area and the sapwood area in the bole. Also we intended to identify the allocation ratio of stem, branches, and leaves or heartwood, sapwood and bark in trees and the characters of productive structure of stem and leaf biomass by the tree height. The results obtained were as follows : 1. The allocation ratio of biomass based on dry weight was 70-84% in stem, 11-25% in branches, and 3-6% in leaves. 2. In the bole, the ratios of composition of heartwood, sapwood, and bark were showed 37-43%, 38-46%, and 16-19%, respectively. 3. The volume of sapmood was exceeded more than that of heartwood in dominant and intermediate trees, while it was reversely appeared in suppressed trees. 4. The weight and area of leases significantly correlated with the sectional area of sapwood in bole (r>0.9. 1% significant level). 5. The ratio of leaf area($m^2$) to sapwood areal($cm^2$), k varied 0.35 to 2.05. 6. The basal diameter and the cross sectional area of a branch significantly correlated with the leaf weight r>0.9. 1% significant level. 7. The leaf weight in a tree is showed a normal distribution curve and the accumulative volume of bole is showed a tapering type.

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Morphological Traits of Selected Chestnut Races and its Propagation Studies (밤나무 우량품종(優良品種)의 형태학적(形態學的) 특징(特徵)과 증식(增殖)에 관(關)한 연구(硏究))

  • Yim, Kyong-Bin;Kwon, Ki-Won
    • Journal of Korean Society of Forest Science
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    • v.22 no.1
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    • pp.49-62
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    • 1974
  • 1. In the comparison of survival ratio among three different methods(bark graft, veneer graft, root graft), bark graft showed the highest survival ratio and root graft was the lowest. It was shown to be significant at the level of 5% in the analysis of variance for the results. It was able to be supposed that one of the main causes for the results was the different amounts of ferric tannic acid which was formated owing to the reaction of grafting knife with tannic acid oozing from cutting faces of the tree. In juvenile tissue graft, the survival ratio of inverted radicle graft was a little higher than that of juvenile stem graft, but there was no significant difference between two methods in analysis of variance. 2. The most hairs of chestnut tree leaves were recognized as stellates on the most part of leaves except for venation. The number of rays in the stellates was ranged from 4 to 8 generally. It was shown to be highly significant differences at the level of 1% among the each race growing at the similar environmental condition in the length of ray and the distributed ratio of the stellates having different ray number. 3. Excepting for the basal width of serration there were no significant differences between $Imakita_1$, $Imakita_2$ as well as between $Teteuchi_1$, $Teteuchi_2$ at the each point of experimental items in this study. Such results made this study more useful. 4. Among the races that were growing in the similar environmental condition, there were highly significant differences at the level of 1% in the length and the width of serration. 5. The rolling of hair, the angle of serration from the leaf margin, the existence of lateral vein in the serration, the intrusion of main vein into the serration and the width of main vein were observed to be somewhat useful as the subsidiary methods for the identification of chestnut races.

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Studies on the Control of the Pine Bark Beetle (Myelophilus pinipedera LINNE) (I) -Analysis of the carotenoids in important pine species in Korea- (소나무좀 방제(防除)에 관한 연구(硏究)(제1보(第1報)) -한국산(韓國産) 중요(重要)소나무류(類) 잎에 함유(含有)되어 있는 Carotenoid 분석(分析)-)

  • Kim, Young Ho
    • Journal of Korean Society of Forest Science
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    • v.27 no.1
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    • pp.15-21
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    • 1975
  • According to Yoshihisa Kuroki's report that the total amount of carotenoid was more in the susceptible to pine bark beetle, than in the resistant ones, carotenoids were extracted from needle leaves of one year old seedlings of Pinus thunbergii, Pinus desiflora, Pinus rigida, Pinus koraiensis, Pinus rigitaeda and Pinus taeda which are all important pines in Korea, to find their resistant ratio to the insect. The carotenoids were analyzed and compared using the spectra of them by spectrophotometer. The results were as follows: 1. The visible absorption spectra of carotenoids in those pine trees were proved to be very similar. 2. The total amount of carotenoids in needles differed with the tree species and the contents were arranged in decreasing order P. koraiensis>P. rigida>P. thunbergii>P. rigitaeda>P. taeda, it seemed that there was corelation between the cartenoid content and the extent of harm caused by the pine bark beetles except P. koraiensis. 3. But carotenoids were contained in Robinia pseudoacacia, Castanea crenata, Chamaecyparis obtusa and Cedrus deodra leaves too. 4. The total amounts of carotenoids in these pines of 9 species were arranged in decreasing order Robinia pseudoacacia>Pinus koraiensis>Pinus rigida>Pinus thunbergii>Castanea crenata>Pinus rigitaeda>Pinus taeda>Chamaecyparis obtusa>Cedrus deodara. Therefore, it was proved that there was no correlation between carotenoid cotent and extent of resistance to the insect. 5. In the thin-layer chromatography of these carotenoids, 13 kinds of components in P. densiftora, P. koraiensis and P. rigida and 12 kinds of spots in other pines, were detected respectively, under ultra-violet fluorescent lamp $3,600{\AA}$ and $2,537{\AA}$. 6. The eighth spots from the bottom in P. densiflora, P. koraiensis and P. rigida were not found in other pines and other 4 species (Robinia pseudoacacia, Castanea crenata, Chamacyparis obtusa, Cedrus deodra). Especially the spot in P. densiflora fluoresced strong cobalt blue-fluorescence under ultra-violet fluorescent lamp $2,537{\AA}$.

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Stimulation of Flowering in Chamaecyparis obtusa Grafts by Gibberellin Treatments (Gibberellin 처리(處理)에 의(依)한 편백나무의 개화촉진(開花促進))

  • Kim, Won Woo;Kim, Zin Suh
    • Journal of Korean Society of Forest Science
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    • v.87 no.4
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    • pp.549-556
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    • 1998
  • To develop the effective methods of flowering stimulation, Hinoki cypress (Chamaecyparis obtusa Sieh. et Zuec.) grafts growing in a seed orchard and in a clone bank in Southern Breeding Station of Cheju were applied with gibberellin treatments, and predicted the seed production potential. In the seed orchard, $GA_{4/7}$ 1.5cc was injected into the stem of drafts and sprayed whale tree crown with $GA_3$ 300ppm and $GA_{4/7}$ 300ppm. Un the other hand, in the clonal archives, drafts were given intrusion of $GA_{4/7}$ 1.5cc into the excised and open inner part of bark wind $GA_3$ 20mg and sprayed with $GA_3$ 300ppm. Additionally, grafts growing in the seed orchard were treated with gibberellins at 3 different periods of time and 3 different treatments during the growing season. The results obtained here are summarized as follows : 1. All of the applications of Gilbberellin promoted female flower formation. Among these, the treatment of intrusion of $GA_{4/7}$ 1.5cc into the excised and open inner part of bark was racist effective, followed by the spraying of $GA_3$ 300ppm. Similarly, the applications of gibberellin promoted male flower formation. 2. Regarding the time of applications, treatment on August 15 was more effective than those of August 31 and September 11 in the stimulation of female flowers. On the contrary, there was no significant difference in the number of male flowers among 3 different time treatments. 3. It was supposed that the application of the intrusion of $GA_{4/7}$ 1.5cc into the excised and open inner part of bark on August 15 showing the best effect in female flower formation can produce 22.12kg seeds per ha. 4. Considerable significant difference existed among clones for both female and male flower formations. 5. Flower formation, especially female flower formation, seemed to be partially associated with the genetic potential of individual trees.

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Assessment of Growth Conditions and Maintenance of Law-Protected Trees in Je-cheon City (제천시 보호수의 생육환경 및 관리현황 평가)

  • Yoon, Young-Han;Ju, Jin-Hee
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.28 no.2
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    • pp.67-74
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    • 2010
  • Law-protected trees are our precious asset as natural resources with history and tradition and natural heritage which should be protected and maintained well to bequeath next generation. Law-protected trees have not only thremmatologic and genetic meaning but also environmental and emotional meaning for their value to be high. This study investigated location, vitality, wrapping condition of root area and status of maintenance of the trees to figure out their growth environment and status of maintenance in a small-middle city through survey on those of law-protected trees in Je-cheon. There showed 300 more year old trees in Je-cheon mostly and the number of trees located in flat fields was the highest. For location type, village, hill and road types were presented in the order and for degree of development, land for building was found most frequently. The average electric resistance of the formative layer was measured to be $8.4k{\Omega}$ and four trees showed bark separation. Most law-protected trees underwent tree surgery, and complete bareness of root area was observed in a tree. The root area of two trees was covered with concrete. pH of soil was recorded to be 5.0~8.4 with its average of 7.1 and electric conductivity(EC) was less than 0.5 dS/m. For status of maintenance rearing facilities were placed for 16 trees out of totally 48 ones and stone fence was done for three ones. Tree surgery was conducted for 33 trees to prevent and to treat decomposed parts of holes. Direction boards were installed for 23 trees. Based on these results, measures to manage systematically law-protected trees in Jecheon could be suggested as follows. First, a sufficient space for growth of low part of trees should be secured. Second, a voluntary management should be induced by advertising them to residents in a community. Third, rearing facilities and direction boards of law-protected trees should be placed and related education should be conducted. Fourth, through operation of the department for law-protected trees consisting of related professions and cooperation among related departments the trees should be maintained continuously.

The Trend and Achievements of Forest Genetics Research in Abroad (선진국(先進國)에 있어서의 임목육종연구(林木育種硏究)의 동향(動向))

  • Hyun, Sin Kyu
    • Journal of Korean Society of Forest Science
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    • v.14 no.1
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    • pp.1-20
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    • 1972
  • The trend and achievements of forest genetics research in abroad were investigated through observation tours and reference work and following facts were found to be important aspects which should be adopted in the forest genetics research program in Korea. Because of world wide recognization on the urgency of taking a measure to reserve some areas of the representative forest type on the globe before the extingtion of such forest type as the results of continuous exploitations of the natural forests to meet the timber demand all over the world, it is urgently needed to take a measure to reserve certain areas of natural stand of Pinus koraiensis, Pinus parviflora, Pinus densiflora f. erectra, Abies koreana, Quercus sp., Populus sp., etc. as gene pool to be used for the future program of forest tree improvement. And the genetic studies of those natural forest of economic tree species are also to be performed. 1. Increase of the number of selected tree for breeding purpose. Because of the fact that the number of plus tree at present is too small to carry out selection program for tree improvement, particularly for the formation of source population for recurrent selection of parent trees of the 2nd generation seed orchard it is to be strongly emphasized to increase the number of plus tree by alleviating selection criteria in order to enlarge the population size of plus trees to make the selection program more efficient. 2. Progeny testing More stress should be placed on carrying out progeny testing of selected trees with open pollinated seeds. And particular efforts are to be made for conducting studies on adult/juvenile correlation of important traits with a view to enable to predict adult performances with some traits revealed in juvenile age thus to save time for progeny testing. 3. Genotype-environment interaction Studies on genotype and environment interaction should be conducted in order to elucidate whether the plus trees selected on the good site express their superiority on the poor site or not and how the environment affect the genotype. And the justification of present classification of seed distribution area should be examined. 4. Seed orchard of broad leaf tree species. Due to the difficulty of accurate comparison of growth rate of neighbouring trees of broad leaf tree species in natural stand, it is recommended that for the improvement of broad leaf trees a seedling seed orchard is to be made by roguing the progeny test plantation planted densely with control pollinated seedlings of selected trees. 5. Breeding for insect resistant varieties. In the light of the fact that the resistant characteristics against insect such as pine gall midge (Thiecodiplosis japonensis U. et I.) and pine bark beetle (Myelophilus pinipera L.) are highly correlated with the amount and quality of resin which are known as gene controlled characteristics, breeding for insect resistance should be carried out. 6. Breeding for timber properties. With the tree species for pulp wood in particular, emphasis should be placed upon breeding for high specific gravity of timber. 7. Introduction of Cryptomeria and Japanese Cypress In the light of the fact that the major clones of Cryptomeria are originated from Yoshino source and are being planted up to considerably north and high elevation in Japan, those species should be examined on their cold resistance in Korea by planting them in further northern part of the country.

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Estimation of Carbon Storage for Pinus rigida Stands in Muju (무주지역 리기다소나무 임분에 대한 탄소저장량 평가)

  • Seo, Yeon Ok;Jung, Sung Cheol;Lee, Young Jin
    • Korean Journal of Environment and Ecology
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    • v.30 no.3
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    • pp.399-405
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    • 2016
  • This study aimed to determine the total amount of carbon stored in the aboveground, belowground tree biomass, dead trees, and organic matter in the forest floor and soil of the 36-year-old Pinus rigida stands in Muju. A total of three plots were selected to measure the carbon stored in live trees, dead trees, forest floor, and soil. Results showed that the carbon stocks of P. rigida stands amounted to 51.0 ton C/ha in aboveground biomass and 29.6 ton C/ha in belowground biomass. The distribution ratios of carbon stocks were as follows: 41.0% in stem wood, 36.8% in roots, 12.8% in branches, 6.0% in stem bark and 3.4% in foliage. The carbon stocks in dead tree amounted to 0.65 ton C/ha while it was 6.40 ton C/ha in organic matter in forest floor. The total amount of carbon stocks found in soil was 51.62 ton C/ha: 20.27 ton C/ha at 0~10 cm depth, 12.83 ton C/ha at 10~20 cm depth, 12.27 ton C/ha at 20~30 cm depth, and 6.24 ton C/ha at 30~50 cm depth. It was also observed that, as the soil depth increased, the soil carbon stocks tended to decrease. Results showed that the total amount of carbon stocks of P. rigida stands in Muju was 139.27 ton C/ha; the highest portion of the cumulative carbon stocks was found to be in soil at 37.1%, followed by the aboveground biomass with 36.6%, belowground biomass with 21.3%, forest floor with 4.6 % and dead trees with 0.5 %. This study is expected to provide forest managers accurate estimates of carbon stored in the habitat of P. rigida stands in Muju.

β-Lapachone-Induced Apoptosis is Associated with Inhibition of Cyclooxygenase-2 Activity in Human Lung Cancer A549 Cells (β-lapachone에 의한 A549 인체폐암세포의 apoptosis 유도와 cyclooxygenase-2 활성 저하)

  • Choi, Yung-Hyun
    • Journal of Life Science
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    • v.21 no.10
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    • pp.1494-1499
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    • 2011
  • ${\beta}$-lapachone, a quinone of lapachol extracted from the bark of the lapacho tree, has been found to induce apoptosis in various human cancer cells. In the present study, we investigated further possible mechanisms by which ${\beta}$-lapachone exerts its pro-apoptotic action in cultured human lung cancer A549 cells. ${\beta}$-lapachone treatment resulted in inhibition of growth and induction of apoptosis in a concentration-dependent manner, as determined by MTT assay and flow cytometry analysis. The induction of apoptosis by ${\beta}$-lapachone was associated with up-regulation of the expression of p53 and p21 in both transcriptional and translational levels, and the phosphorylation of p53. In addition, ${\beta}$-lapachone activated caspase-3 and -9, and induced degradation of caspase-3 target proteins such as poly (ADP-ribose) polymerase (PARP) and ${\beta}$-catenin. Furthermore, ${\beta}$-lapachone treatment caused a progressive decrease in the expression levels of cyclooxygenase (COX)-2 without significant changes in the levels of COX-1, which was correlated with a decrease in prostaglandin E2 synthesis. Taken together, these results indicated that ${\beta}$-lapachone may have therapeutic potential in human lung cancer treatment.