• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.704-709
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• 1996

This experiment was carried out to establish the system of mass propagation through tissue culture using sucker and stem in Ramie. The sterilization for tissue culture of Ramie was the better treatment of 2% NaClO for 20 minute into ultrasonic cleaner than the others, and so rate of contamination was 3.3%, and it was able to produce 96% healthy plant. The effect of growth regulator was superior to mixed treatment of 0.02mg/$\ell$ NAA, 1.5mg/$\ell$ BA, 0.lmgmg/$\ell$ GA$_3$, which it was not formed callus and but produced 96% healthy plant. The effect of propagation was higher in culturing of the stem node than the sucker in cultural part, local variety than improved ones. The effect of acclimatization was superior to pretreatment of 30 minute after soaking in 100ppm NAA, transplanting on bed soil which mixed to ratio of vermiculite : soil : sand =1 : 2 : 1, the transplanted plants were grown all normal.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.280-283
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• 2007

A 6-year-old male dog was referred to Animal Medical Center, Chonbuk National University with large distal extremity abrasion wound to the hind limb by automobile accident. The wound was reconstructed with an autogenous, full-thickness skin mesh graft. The graft was harvested from the ventrolateral abdominal wall and transplanted to the wound. The skin mesh graft survived successfully without any noticeable complications. Successful grafting requires asepsis, an adequately prepared recipient bed through the surgical debridement and lavage, proper harvesting and preparation of the graft. Full-thickness skin mesh graft has lesser skin contraction which prevents formation of scar. Meshing the graft provides more graft flexibility over uneven, convex and concave surfaces and allows adequate drainage. The full-thickness skin mesh graft can be successfully used for the treatment of large distal skin wounds in dogs.

• Korean Journal of Medicinal Crop Science
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• v.26 no.6
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• pp.464-470
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• 2018

Background: Ginseng produced by hydroponics can be cultivated without using agricultural chemicals; thus, it can be used as a raw materials for functional foods, medicines, and cosmetics. This study aimed to determine the optimal harvesting time to obtain the highest levels of ginsenoside and ginseng, as this was not previously unknown. Methods and Results: One-year-old organic ginseng seedlings were transplanted and cultivated using hydroponics for 150 days in a venlo-type greenhouse, using ginseng nursery bed soil and a nutrient solution ($NO_3{^-}-N$; 6.165, P; 3.525, K; 5.625, Ca; 4.365, Mg; 5.085, S; $5.31mEq/{\ell}$). Ginsenoside content and fresh and dry weights were higher at 120 days after transplanting than at 30, 60, 90, and 150 days. Total ginsenoside content was 11.86 times higher in the leaf and stem than in the root at 120 days after transplanting. Ginsenosides F1, F2, F3, and F5 were detected in ginseng leaves and stems. These chemical compounds are known to be effective in altering skin properties, including whitening, anti-inflammation, and anti-aging. Conclusions: Optimal harvesting time for ginseng cultivated using hydroponics was 120 days after transplanting when the biomass and ginsenoside content were highest.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.11
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• pp.1-9
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• 1972

For a measure against late transplanting, this experiment was conducted to investigate a reasonable seeding rate in nursery bed and a proper nursery size. The treatments applied to this experiment are as follows a) seeding rates: 0.2ι, 0.4ι and 0.6ι per 3.3$m^2$ b) nursery sizes. for 10a-field area: 39.6$m^2$, 49.5$m^2$ and 59.4$m^2$ c) transplanting dates: June 5, June 25 and July 15. The seeding date was April 26 and planting density was determined by the number of sound seedlings based on the combination of seeding rate and nursery size. The results may be summarized as follows: 1. In seedlings both increased nursery period and decreased seeding rate showed a remarkable-increase in plant height, number of leaves, dry matter weight and the ratio of dry weight to plant height. But their number of tillers and live leaves did not show such tendency. 2. Delayed transplanting date shortened culm length and panicle length as well as number of days from transplating to heading and it also delayed heading date. On the other hand the transplanting of 80-day seedlings resulted in premature heading. 3. As a result late transplanting reduced number of spikelets per panicle, maturing rate and 1000-grain weight. In the last analysis it linearly reduced grain yield. The decreasing rate of yield by late transplanting was 15.6% in June 25 plot and 41. 3% in July 15 plot, compared with the yield in June 5 plot. Such a remarkable decrease in yield of the extremely late transplanted plot was mainly due to markedly decreasing number of spikelets per panicle and 1000-grain weight. 4. Both increased seeding rate and nursery size gave a rise in number of tillers per unit area as well as number of transplanting hills but gave a fall in culm length and panicle length. 5. Accordingly, though thick seeded - dense planted plot increased number of panicles per unit area, decrease in number of spikelets per panicle and 1000-grain weight made no differences in yield between thick seeded - dense planted plot and thin seeded - sparse planted one. However, the yield in the thick seeded - dense planted plot transplanted on July 15 was reduced owing to the remarkable decrease in maturing rate and 1000-grain weight. 6. We came to the conclusion that as a measure against the extremely late transplanting the suitable seeding rate was 0.4ι per 3.3$m^2$ and the proper nursery size was 59.4$m^2$ for 10a-field area.

• The Korean Journal of Ecology
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• v.16 no.4
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• pp.377-395
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• 1993

Habitat types, community structure and population characteristics of Koelreuteria paniculata were investigated in Mt. Wolak, Chungbuk and Naesokdong, Daegu, which are natural habitats of the species in inland region of Korea, and its origin was discussed. Habitats of Koelreuteria paniculata were classified to 3 types: sand bar formed by the sands flooded in the course of flow of the mountain stream (Type 1). crevice on the rock bed within the mountain stream (Type 2) and crevice of the rock around the edge of mountain stream (Type 3). Most Koelreuteria paniculata communities in Mt. Wolak site were composed of 3 layers of subtree layer, shrub layer and herb layer and that of Daegu site was 4 layers including tree layer. In the floristic composition of the Koelreuteria paniculata community, plants occurring frequently in the wet and open site, such as Zelkova serrata and Fraxinus rhynchoph-vlla showed high frequency. Frequency distribution of diameter at ground surface of Koelreutrria paniculata showed reversed J-shaped type. It was supposed that expansion of Koelreuteria paniculata community in Mt. Wolak site might be accomplished by the flow of the stream. Many saplings capable of becoming a successor of mature trees in Daegu site in near degenerating phase were established on the forest floor of the Koelreuteria paniculata community. From this result, it was supposed that these saplings originated from the seeds dispersed from a seed tree might form the Koelreuteria paniculata community of the next generation. On the other hand, the origin of Koelreutevia paniculata in inland sites was explained by two hypotheses: the one was that Koelreuteria paniculata might be transplanted by human and the other was that the present site might be native habitat of the community.

• Korean Journal of Environmental Biology
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• v.30 no.1
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• pp.47-53
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• 2012

To evaluate adaptation success of $Zostera$ $caespitosa$ transplants, we transplanted the seagrass shoots at the bare area in close proximity to the donor bed using staple method in October 2005. Shoot density, morphological characteristics and leaf productivity of transplants and reference plants in the vicinity of the planting site were monitored monthly for 2 years. While shoot density of reference plants exhibited significant seasonal variations; increasing during spring and summer and decreasing during fall and winter, that of transplants increased consistently without initial loss during the whole study period. Although sheath length, leaf width and shoot height and weight of sheath, leaf and shoot of transplants were smaller than those of reference plants at the start of transplantation, increased rapidly reaching even higher values than those of reference plants 5 months after transplantation. Leaf productivity of transplants and reference plants showed seasonal variations; increasing during spring and summer and decreasing during fall and winter. But, leaf productivity of transplants increased at the beginning of transplantation during fall which is low production period. All of the $Z.$ $caespitosa$ transplants survived during the whole study period. Rapid changes in shoot morphology and growth of transplants indicated that $Z.$ $caespitosa$ transplants had great morphological plasticity and adapted successfully within 5 months.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.325-328
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• 2001

This experiment was conducted to investigate the effect of plug cell volume and medium on rooting and growth of lateral shoot cuttings of a few tomato cultivars. Plug cell volume was varied from 23 to 300 mL or control (cutting bed) and media used were carbonized rice hull (CRH), CRH+perlite, CRH+peatmoss and perlite+peatmoss. Nursery plants were able to be transplanted in 15 to 20 days after lateral shoots cutting in tomato. In volume of cutting media, the pots of 23 to 300 mL were proper, although root growth was gradually limited in decreased media volume. Rooting and growth was not influenced by plug tray cell medium of 120 mL or more. Cherry tomato Pepe (c.v.) showed 100% rooting and better growth, while in Momotaro (c.v.) rooting was 90%. There were 100% rooting and no considerable changes grown in all media tested of cutting plug tray volume of 30 mL.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.275-280
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• 1999

In order to find optimum conditions for somatic embryogenesis from different individual (2-year-old) in Aralia elata were cultured on MS medium supplemented with 1.0 mg/L 2,4-D, 3% sucrose, and 0.3% gelrite. We also investigated the effect of MS medium salt concentration, BA and ABA on the embryo germination and plant regeneration. While noticeable difference was observed on somatic embryo induction among different individual tree, no apparent difference was seen in both germination and regeneration frequencies. Compared with nonembryogenic calli, embryogenic calli tended to look yellow and/or pale brown in color, slowly growing and soft in their texture. Regardless of BA or ABA treatment, half-strength MS salt medium proved to be better than full strength MS medium in both embryo germination and plant regeneration. Both hypocotyl and cotyledon developments were slightly promoted by adding 0.1 mg/L BA. However, its effect on germination and regeneration seemed inferior to control. ABA treatment on somatic embryos at their torpedo and early cotyledonary stages resulted in poor response in germination and regeneration. Although most regenerated plantlets varied greatly in cotyledon number and shape, they could be developed into normal plants after 4 weeks in culture. More than 95% plantlets were acclimatized in an artificial soil mixture, successfully transplanted to nursery bed and grew normally without any phenotypic abnormalty.

• Research in Plant Disease
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• v.23 no.2
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• pp.186-192
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• 2017

To survey the incidence of plant-parasitic nematode in strawberry nursery, 117 soil samples were collected from main producing area of strawberry nursery plants in 2016. The incidences of two major problematic nematodes such as root-lesion nematodes (Pratylenchus spp.) and root-knot nematodes (Meloidogyne spp.) were 11% and 3%, respectively. Strawberry nurseries using upland soil either in fields or in plastic-houses showed were higher infection rates of Pratylenchus spp. or Meloidogyne spp. than others. On the other hand, a strawberry nursery using pots filled with bed soil in plastic-house was the lowest infection rate with 0%. Phylogenetic relationships and distance analysis were performed for identification of Pratylenchus spp. and Meloidogyne spp. detected from strawberry nurseries. As the results, they were identified as Pratylenchus penetrans, Pratylenchus vulnus and Meloidogyne hapla. Meanwhile, when nursery plants of strawberry infested with P. vulnus transplanted to pots filled with sterilized soil, P. vulnus was detected from soil in pots as 33% frequency. Thus, P. vulnus can be spread through nursery plants of strawberry infested with that. These studies suggested that Pratylenchus spp. and Meloidogyne spp. were ploblematic nematodes in major areas of strawberry nursery plants and P. vulnus could be spread through nursery plants of strawberry.

• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.137-142
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• 1999

This study was carried out to investigate the effects of bulb size, type of media, depth of planting, and nutrient compositions on sprouting and growth of tissue cultured garlic microbulbs in hydroponic culture. Early and increased sprouting were observed when the microbulbs were transplanted into soil planted in shallow (1 cm in depth), while bulb size and fresh weight of the whole plant increased in deep planting (3 cm in depth). Bulb size have greatly influenced on not only sprouting rate but also plant growth after planting. Large bulbs resulted in high growth rate such as number of leaves, stem width, plant height, and increase in bulb size after planting. It was shown that Oriental nutrient solution (N=0.17, P=0.45, K=1.29, Ca=2.44, and Mg=0.98 me/L) was more effective in sprouting and further growth of microbulbs as compared to Yamazaki solution (N=0.27, P=0.16, K=1.50, Ca=1.36 and Mg=0.78 me/L). Microbulbs grown in mixture of leaf mould + bark + sand or Baroko showed the most vigrous growth.