• Archives of Reconstructive Microsurgery
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• 제21권2호
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• pp.131-136
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• 2012

Purpose: Arterialized venous flap is useful for reconstruction of the traumatic soft tissue defect in fingers, but insufficient circulation of the traumatic fingers makes surgeons annoying to use the flap. We have grafted flaps in 7 fingers with insufficient vascular bed hoping to expanded the category of the flap. Materials and Methods: Arterialized venous flap have transplanted in 7 fingers from March 2008 through February 2010 and followed up for 4 to 16 months(average 7.2 months). They were all male with a mean age at the time of surgery was 33. The main injury was crushing in 4 degloving, contact burn and saw injury was I respectively. Time interval from injury to flap transplantation was average 3.1. weeks(3 days to 6 weeks). Designed flap size ranges from $8cm{\times}3.5cm$to $4cm{\times}3cm$. Vessel type of flap was one artery with two veins were 5 cases and one artey with one vein 2. Flap type was cutaneous in 3, tendocutaneous 2, neurotendocutaneous 1 and neurocutaneous 1. The circulation state of recipient site was avascular in 2 cases, insufficiency 3 and tip avascular 2. Results: Arterialized venous flap was complete survived in 2 cases, partial necrosis(less than 10%) 3 and failed in 2. Conclusion: An arterialized venous free flap could be a useful procedure for reconstruction in soft tissue or combined defect of the finger despite an avascular or insufficient vascular beds if the recipient beds were free from infection.

• 식물병연구
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• 제9권2호
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• pp.64-67
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• 2003

배추 뿌리혹병을 방제하기 위하여 상토의 종류와 농약 병용처리에 대한 효과를 조사한 결과 온실시험에서 피트 모스, 바로커 상토에 육묘한 배추에서의 발병도가 48.3, 54.2%에 비해 황토, 마사토 상토에 육묘한 배추에서는 발병도가 10.8, 18.7%이었고 포장시험에서는 피트모스 상토 75.7%, 바로커 36.6$, 마사토21.7%, 황토 14.1% 순이었다. 후루설파마이드 분제를 토양에 혼화처리하였을때는 봄재배에서 피트모스 상토에서는 발병도 4.5%에 비해 황토 상토에서는 0.4%이었고 가을재배에서는 10.1, 5.7%이었다. 이상의 결과에서 피트모스, 바로커 상토보다 황토, 마사토 상토를 사용하고 후루설파마이드 분제로 약제처리하면 뿌리혹병 방제에 효과적일 것으로 생각된다.

• 한국산림과학회지
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• 제76권1호
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• pp.1-10
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• 1987

잣나무의 유묘기(幼苗期) 생육(生育)에 있어 광(光)과 단근(斷根) 처리(處理)에 따른 이식후(移植後) 생장(生長)에 미치는 몇 가지 영향(影響)에 대하여 조사(調査), 비교(比較) 하였다. 기(其) 결과(結果) 묘목(苗木)의 활착율(活着率)은 상대광도(相對光度)가 낮은 19%구(區)가 가장 높았고, 단근량(斷根量)은 1/2 이상(以上)이 되면 50% 이상(以上)이 감소(減少)되었다. 지상부(地上部), 지하부(地下部) 및 묘경생장(苗徑生長)은 상대광도(相對光度) 100%에서, 그리고 단근(斷根) 1/4 처리구(處理區)가 가장 양호(良好)하였다. 또한 엽속수(葉束數) 및 엽면적(葉面積)은 상대광도(相對光度) 63%구(區)가, 그리고 단근(斷根) 1/4 처리구(處理區)가 가장 크게 나타났다. 따라서 잣나무 유묘생장(幼苗生長)에 미치는 광도(光度)의 영향(影響)은 비교적(比較的) 높았으나 단근량(斷根量)의 효과(效果)는 극(極)히 적게 나타났다.

• Journal of Biosystems Engineering
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• 제35권1호
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• pp.37-45
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• 2010

Since SRC (Short-term Rotation Coppice) such as poplar and willow can be harvested in three years, they are known to be a potential forest biomass as fuel for a power plant. The production system including transplanting and harvesting is, however, necessary to be mechanized because such a biomass should be handled in a massive volumetric size. A pull-type stem-cutting transplanter was developed in the research as the first step to realize the production of SRC. A needle-like transplanting device pushes a stem-cutting into the prepared soil bed by a pneumatic cylinder, and another device firms soil around a stem-cutting transplanted. Since this is an intermittent operation, it was necessary to develop a zero horizontal velocity mechanism which enabled only the transplanting needle part to continue a zero horizontal movement relative to the ground during the transplanting operation even when the tractor kept moving forward. The 2-row transplanter can transplant stem-cuttings at the rate of 6.5 seconds per row without missing a single attempt. The planting depth and distance were well maintained and controlled. Their CVs were between 2.1~3.4% and 0.87~1.7% for the depth and the distance, respectively. Although, the transplanted stem-cuttings tended to lean outward from the back-view and forward from the side view, they were planted within the range of $3^{\circ}$ from the upright position.

• 한국약용작물학회지
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• 제13권4호
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• pp.133-137
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• 2005

가시오갈피 체세포 배를 이용한 우량종묘의 대량생산체계를 확립할 목적으로 체세포 배의 생물반응기 배양을 통해 얻은 식물체의 순화에 관한 기초시험을 수행하였다. 가시오갈피 기내배양 식물체의 토양활착은 밀폐된 공간보다 비가림 하지 않은 차광막조건에서 양호하였고 순화에는 Klasmann 상토와 원예용상토가 좋았다. 배양실 10도와 차광막에서 3월20일 순화한 기내식물체의 순화 율은 모두 90% 이상으로 양호하였다. 배양실조건에서는 $26^{\circ}C$의 순화온도에서는 10% 이하의 극히 저조한 순화 생존율을 보였으나 포장 차광막조건에서는 최고 온도가 $30^{\circ}C$를 넘는 5월20일 순화 처리구에서도 68%의 높은 순화생존율을 보여 불리한 환경조건에서 차광막순화가 순화에 더욱 적합하였다. 차광조건으로는 50% 차광망 처리가 적합하였다. 차광막에서 순화한 식물체의 묘 소질은 3월10일 순화를 시작한 식물체가 가장 좋았다.

• Journal of Periodontal and Implant Science
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• 제31권1호
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• pp.193-209
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• 2001

The prognosis of transplanted teeth is strongly related with periodontal healing. Several experimental studies showed that the application of enamel matrix derivatives on periodontitis-affected root surfaces resulted in periodontal regeneration. The purpose of this study was to determine the effect of enamel matrix derivatives on periodontitis-affected root surfaces prior to transplantation in dogs. Class III Furcation defects were surgically created on the left second, the third and the fourth premolar in the mandibles of nine mongrel dogs and experimental periodontitis was induced by placing small cotton pellets into defects for 3 weeks. Periodontitis-affected roots were treated by scaling and planing and the coronal portions were removed. Each root was extracted and implanted into recipient bed prepared in the contralateral premolar area. The transplanted roots were grouped according to the treatment modalities; Group I- roots treated with saline only, Group II- roots conditioned by neutral EDTA, and Group III- roots conditioned by neutral EDTA and enamel matrix derivatives ($EMDOGAIN^{(R)}$, BIORA Co., Sweden). The animals were sacrificed at 1 week, 3 weeks, and 10 weeks after transplantation and decalcified specimens were prepared for histologic examination. In Group I, healing was most frequently characterized by root resorption and ankylosis. In Group II, with root resorption and ankylosis in a few specimens, connective tissue attachment was partly seen on denuded root surface, but no cementum formation was seen. In Group III, there was regeneration by new cementum and periodontal ligament on denuded root surface, although slight root resorption and ankylosis were found in a few specimens. This result suggests that enamel matrix derivatives treatment on periodontitis-aggected root surface could reduce the frequency of root resorption and ankylosis and contribute to periodontal regeneration, and might be useful for autologous transplantation.

• 식물조직배양학회지
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• 제28권5호
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• pp.273-276
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• 2001

두릅나무의 체세포배 유래 소식물체의 효과적인 순화 방법을 개발하고자 플라스틱 용기를 사용 4가지 인공배양토 및 MS 액체배지 등 7가지 수용액 처리를 통해 활착률 및 생장을 조사하였다. 상토는 perlite가 vermiculite보다 활착 및 생장에 양호하였고, 입자가 클수록 활착률 및 생장이 좋았다. 액체배지는 2차 증류수 처리가 가장 양호하여 96%의 활착률을 나타냈으며, 염류농도를 1/4 및 1/8로 낮춘 MS배지는 각각 92%의 활착률을 보인 반면 기본배지 및 1/2 MS배지는 활착률이 저조하였다. 한편 0.1% hyponex와 2% sucrose 처리는 84% 및 76%의 활착률을 각각 나타냈다. 결론적으로 두릅나무의 체세포배 유래 소식물체의 순화는 입자가 큰 perlite를 사용하고 증류수 처리 혹은 염류 농도를 낮춘 MS배지 처리로 효과적인 순화가 가능함을 보여주었다.

• 원예과학기술지
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• 제30권2호
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• pp.129-136
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• 2012

왕벚나무 성목(43-58년생)의 삽목묘 대량증식 방법을 밝히기 위해 삽목시기별, 옥신처리별 및 삽수부위별로 자동안개관수장치가 설치된 무가온 비닐하우스에 삽목을 실시했으며, 삽목묘의 가로수 식재 성공여부를 검토하기 위해 발근된 삽목묘를 묘포장에 이식하여 생육특성을 조사하였다. 각 처리에 따른 평균 발근율의 분산분석 결과는 고도의 유의성($P$ < 0.0001)이 나타났다. 시기별 평균 발근율에서 6월 1일의 삽목(61.4%)이 8월 1일(23.6%)보다 2배 이상 높아 6월 1일이 적합한 시기로 나타났다. 발근율이 가장 좋은 옥신 처리는 IBA $1,000mg{\cdot}L^{-1}$와 IBA $500mg{\cdot}L^{-1}$ 처리에서 각각 90.8%, 89.2%로 나타났으며, 발근율에 대한 각 처리간의 상호효과($P$ < 0.0001)가 인정되었다. 삽수부위에서 상단부 + IBA $1,000mg{\cdot}L^{-1}$ 처리가 발근율 96.7%로 가장 높게 나타났다. 삽목묘의 묘포장 이식 활착률 역시 옥신처리간에 고도의 유의성($P$ < 0.0001)이 인정되었으며, 잎이 있는 삽목묘의 평균 이식활착률은 46.5%로 낮았으나 IBA $1,000mg{\cdot}L^{-1}$ 처리는 79.2%로 높았다. 그러나 잎이 없는 삽목묘의 이식활착율은 8.7%로 대부분 고사하였다. 활착된 삽목묘와 접목묘 및 실생묘의 생장특성을 비교한 결과, 생장(수고, 근원경), 뿌리수, 가지수, 잎수 등에서 고도의 유의성($P$ < 0.0001)이 나타났으며 삽목묘가 월등히 우수하였다. 본 연구의 결과는 자동안개관수장치로 안정적인 습도조절과 온도유지가 가능한 삽목실에서 6 - 7월의 삽목실시, 상단부 삽수의 선택, IBA처리 및 8월에 발근된 삽목묘의 묘포장 이식을 통해 개량된 왕벚나무의 대량증식과 성공적인 가로수 식재가 가능할 것이다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.37-37
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• 2018

We use two different methods for laboratory propagation from seed of lady's slipper orchid(Cypripedium macranthos Sw.); immature seed which also called green capsule or fully mature seed about 120~130 days from pollination. In green capsule culture, the seed pods should be collected within precisely right time. The right time of seed collection could be diverse under the wether conditions or nutritional factors of the plants. In fully matured seed culture, the more complicated procedures are needed to break the dormancy of the seed; thermal or chemical treatment. The seedlings in this study were easily germinated from immature seeds in Harvais medium; 53 days after pollination(DAP) in Cypripedium pubescens, DAP 65 in C. parviflorum and C. macranthos. The germinated seedlings were transplanted to hormone free media immediately to avoid abnormal growth of seedlings. When the seedlings have roots with a minimum length of around 2-3cm and have visible dormant buds, the seedlings were removed from the flask and stored in refrigerator for vernalization. To examine the correlation of seedlings and maternal plants, the 125 seedlings of C. macranthos were transplanted in the soil bed at a distance of 20-100 cm from mother plants on April 20. The survival rate of seedlings were 92% in 20 cm distance from the ripe plants, and 56 % in 100 cm distance. The seedlings which were transplanted near mother plants showed vigorous growth in plant height, leaf width, and especially dormant buds. Considering the existence of mycorrhiza which is a symbiotic association between a fungus and the roots of a orchid vascular, the various fungus from mother plants could affect the growth of the seedlings. These results indicate the possibility of high and stable production and practical industrialization of endangered lady's slipper orchids.

• 한국약용작물학회지
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• 제22권3호
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• pp.231-236
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• 2014

This experiment was carried out to establish the optimum cutting time, plant growth regulator, and bed-soil for rooting by greenwood cutting of Schisandra chinensis. Approximately 7 cm long-shoots of greenwood cuttings were transplanted by various cutting times, plant growth regulators, and bed-soils in the plastic-film house. The rooting rate of greenwood cutting was 12.5% in the April 5th, 73.5% in the May 8th, and 75.5% in the August 5th. The number and length of primary root in greenwood cutting were more in the early May than those in the early August. For mass propagation of Schisandra chinensis. using greenwood cutting, shoots were treated with plant growth regulators on May to increase rooting rate. Rooting rate was 100% with IAA $50mg/{\ell}$, 92.9% with NAA $100mg/{\ell}$, and NAA $1,000mg/{\ell}$, for 60 min. To select effective media for rooting, various medias for bed-soil were treated by single and mixture form for 100 days after cutting. Rooting rate was 91.8% in the single treatment of peat moss or decomposition of granite soil, and this result was better than those in other treatment. The treatment by 1 : 1 mixture of peat moss and horticulture bed soil was rooting with 94.0% best rooting rate.