• Journal of Bio-Environment Control
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• v.32 no.4
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• pp.484-491
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• 2023

Tomatoes in greenhouse are a widely cultivated horticultural crop worldwide, accounting for high production and production value. When greenhouse ventilation is minimized during low temperature periods, CO₂ enrichment is often used to increase tomato photosynthetic rate and yield. Plant-induced electrical signal (PIES) can be used as a technology to monitor changes in the biological response of crops due to environmental changes by using the principle of measuring the resistance value, or impedance, within the crop. This study was conducted to investigate the relationship between tomato growth data, vital response, and PIES resulting from CO₂ enrichment in greenhouse tomatoes. The growth of tomato treated with CO₂ enrichment in the morning was significantly better in all items except stem diameter compared to the control, and PIES values were also higher. The growth of tomato continuously applied with CO₂ was better in the treatment groups than control, and there was no significant difference in chlorophyll fluorescence and photosynthesis. However, PIES and SPAD values were higher in the CO₂ treatment group than control. CO₂ enrichment have a direct relationship with PIES, growth increased, and transpiration increased due to the increased leaf area, resulting in increased water absorption, which appears to be reflected in PIES, which measures vascular impedance. Through this, this study suggests that PIES can be used to monitor crops due to environmental changes, and that PIES is a useful method for non-destructively and continuously monitoring changes of crops.

• Korean Journal of Environment and Ecology
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• v.38 no.4
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• pp.426-433
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• 2024

In this study, to estimate the effects of plant-induced surface area increase and physiological activity on fine dust purification, a control group was set up. We utilized both foliage plants (Spathiphyllum wallisii) and artificial plants (shaped like Spathiphyllum wallisii) to measure and compare the purification time for fine dust. The results showed that the time required for fine dust purification in each experimental group decreased by 57-64% for Type AP and 31-32% for Type P compared to the control group. Subsequently, using a Linear Mixed Model (LMM), we tested the interaction between time and each experimental group, revealing statistically significant interactions between surface area increase and time(PM10 : t=3.123, p<0.05, PM2.5 : t=3.180, p<0.05), as well as physiological activity and time(PM10 : t=4.065, p<0.05, PM2.5 : t=4.307, p<0.05), indicating the presence of interactions between each factor and the time variable. Finally, we estimated the efficiency of fine dust purification by plant factors through nonlinear regression analysis. Compared to the control group without purification factors (Type C), it was estimated that surface area increase shortened the purification time by 1.40 times and physiological activity by an average of 1.95 times, resulting in a total 2.74 times shorter purification time. Based on these results, we hypothesized that physiological activity(transpiration and absorption) has a greater impact on fine dust purification than surface area increase(biosorption). Accordingly, we emphasize the importance of vegetation management practices such as pruning and irrigation management in green spaces aimed at fine dust purification.

• Korean Journal of Environmental Agriculture
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• v.22 no.3
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• pp.185-191
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• 2003

The mechanism imparting salt tolerance to crop plants remains still unsolved, although soybean has been classified as a susceptible plant to NaCl. To determine optimum parameters on physiological responses for improving sensitivity of salinity in breeding program, soybean (Glycine max Merr., cv. "Gwan-gan") plants were grown in a greenhouse, treated 20 days after emergence for 7 days with NaCl at 0, 30, 60, and 90mM, corresponding to electric conductivity of 1.2, 4.4, 7.3, and 10.4 dS/m, respectively, and assessed 30 days after treatment. Chlorophyll contents were significantly decreased by NaCl ($0.4{\sim}1.0\;mg/g$) compared to control (1.2 mg/g). Photosynthesis rate by NaCl treatment at $0{\sim}90\;mM$ at flowering stage was ranged from 5.0 (control) to $9.6\;{\mu}mol/m^2/s$. Oxygen for respiration was consumed from 5.4 to $9.7\;{\mu}mol/m^2/s$ so that the ratio of $O_2$ (evolution:consumption) was increased with the increase of NaCl, indicating that $O_2$ consumption seems to go beyond $O_2$ evolution. Water potential of leaf at vegetative stage II was ranged from -0.6 to -1.8 MPa and the highest level was observed at mid-day. Water potential by salt stress was decreased with range of $-2.1{\sim}-2.7MPa$ compared to control. Transpiration was decreased from 17% to 20% by NaCl stress. Water vapor diffusing resistance of intercellular air space was affected significantly, increasing up to $16{\sim}24%$ compared to control by NaCl treatment. Salt-treated soybean tended to accumulate $Na^+$, specially in root, with reduced absorption of N, P, $K^+$, $Ca^{2+}$, and $Mg^{2+}$ contents. Free proline content of soybean leaf as affected by different NaCl concentrations was increased 4.2 times ($184{\sim}434\;{\mu}g/g$) more than control. NaCl also increased activities of nitrate reductase and peroxidase by $28{\sim}161%$ and $3{\sim}22%$, respectively. The results show that physiological characteristics of soybean plants during assay were useful as the best parameters of salt stress or salt tolerance test to improve sensitivity in screening and breeding program among cultivars or germplasms.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.349-354
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• 2013

This study examined a technique for cooling root zone aimed at lowering substrate temperature for sweet pepper (Capsicum annum L. 'Orange glory') cultivation in coir substrate hydroponics during hot season, from the $16^{th}$ of July to $15^{th}$ of October in 2012. The root zone cooling technique was applied by using an air duct (${\varnothing}12$ cm, hole size 0.1 mm) to blow cool air between two slabs during night (5p.m. to 3a.m.). Between the $23^{rd}$ of July and $31^{st}$ of August (hot temperature period), average daily substrate temperature was $24.7^{\circ}C$ under the root zone cooling, whereas it was $28.2^{\circ}C$ under condition of no cooling (control). In sunny day (600~700 W $m^{-2}{\cdot}s^{-1}$), average substrate temperatures during the day (6a.m. to 8p.m.) and night (8p.m. to 6a.m.) were lower about $1.7^{\circ}C$ and $3.3^{\circ}C$, respectively, under the cooling treatment, compared to that of control. The degree of temperature reduction in the substrate was averagely $0.5^{\circ}C$ per hour under the cooling treatment during 6p.m. to 8p.m.; however, there was no decrease in the temperature under the control. The temperature difference between the cooling and control treatments was $1.3^{\circ}C$ and $0.6^{\circ}C$ in the upper and lower part of the slab, respectively. During the hot temperature period, about 32.5% reduction in the substrate temperature was observed under the cooling treatment, compared to the control. Photosynthesis, transpiration rate, and leaf water potential of plants grown under the cooling treatment were significantly higher than those under the control. The first flowering date in the cooling was faster about 4 days than in the control. Also, the number of fruits was significantly higher than that in the control. No differences in plant height, stem thickness, number of internode, and leaf width were found between the plants grown under the cooling and control, except for the leaf length with a shorter length under the cooling treatment. However, root zone cooling influenced negligibly on eliminating delay in fruiting caused by excessively higher air temperature (> $28^{\circ}C$), although the substrate temperature was reduced by $3^{\circ}C$ to $5.6^{\circ}C$. These results suggest that the technique of lowering substrate temperature by using air-duct blow needs to be incorporated into the lowering growing temperature system for growth and fruit set of health paprika.

• Korean Journal of Agricultural Science
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• v.15 no.1
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• pp.36-46
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• 1988

Twelve soybean cultivars were cultivated in the 1/2,000a. Wagner pots with irrigation and without irrigation for 30 days after flowering, and the differences of plant growth and bean yield among cultivars were compared. And to investigate the varietal differences in the rate of photosynthesis under different relative humidity, 6soybean cultivars were cultivated in 1/2,000a. Wagner pot and the rate of photosynthesis of each soybean cultivar at flowering time was measured under the relative humidity of 80, 70, 60, 50 and 40%. The results obtained are summarized as follows; 1. The days to maturity of the soybean cultivars were shortened by non-irrigation treatment. The response of the maturing dates to non-irrigation was significantly different among the soybean cultivars. The days for maturing of Paldal, Danyeob and Eundaedu were delayed 2 days but those of Jangbaek and Tamahomare were delayed about 7 to 8 days under non-irrigation treatment. 2. The stem length, stem diameter, number of nodes of the mainstem, number of branches and number of branch nodes of all soybean cultivars were decreased by non-irrigation treatment. The number of branches and the number of branch nodes were especially severely influenced by non-irrigation treatment. 3. The number of pods per plant and the number of perfect pods was significantly reduced by non-irrigation treatment but the number of imperfect pods was increased. The non-irrigation treatment reduced the number of pods per plant by 58.0% and the ratio of the number of the perfect pods per plant by 46.6% relative to the ordinary cultivation with irrigation. 4. The grain yield of all cultivars was significantly reduced by the non-irrigation treatment, and average grain yield of soybean cultivars cultivated under non-irrigation treatment was 35.9% of that of soybean cultivars cultivated with irrigation. The influence of non-irrigation treatment was lowest in Paldal and significantly high in Tamahomare and Jangbaek. 5. The rate of photosynthesis of soybean leaves was significantly different among cultivars and was also influenced by relative humidity. Ratio of the photosynthetic amount of soybean leaves at 40% RH to the maximum photosynthesis at optimal humidity was 97.2% in Paldal, 96.4% in Danyeob and 88.8% in Baekun. 6. At 40% relative air humidity, highly significant correlations were found among the photosynthesis rate, the amount of transpiration and the respiration rate.

• Korean Journal of Environment and Ecology
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• v.28 no.5
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• pp.500-509
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• 2014

To prevent freezing of the road by fallen snow, Calcium chloride($CaCl_2$) as a deicer is used to very often and it can be harmful to roadside trees. This study was conducted to investigate the effects of Calcium chloride($CaCl_2$) as a deicer on growth and physiological traits of Acer triflorum according to different concentration of $CaCl_2$. We measured growth, chlorophyll contents, gas exchangement characteristics, chlorophyll fluorescence and mineral nutrition concentration in plant and soil. The experimental group was composed of four treatments including 0mM(control), 9mM(0.5 %), 18mM(1.0 %), 54mM(3.0 %). Before germinating new shoot, the dissolution of $CaCl_2$ was irrigated twice interval of a week. At 30 days after treatment, all treatments decreased total cholorophyll content, photosynthetic rate, transpiration rate, stomatal conductance and photochemical efficiency($F_v/F_m$) with increasing concentration of $CaCl_2$ and especially, they significantly reduced in 3.0 % treatment. In contrast, chlorophyll a/b ratio increased with an increase of $CaCl_2$ concentration and water use efficiency increased in 1.0 % and 3.0 % treatments. At 50 days after treatment, all treatments were decreased in chl a, chl b, total chlorophyll content, carotenoid content, photosynthetic capacity, photochemical efficiency($F_v/F_m$) and quantum yield of photosystem II(${\Phi}_{PSII}$) compared with control and 3.0 % treatments were withered. $Ca^{2+}$ and $Cl^-$ were accumulated in leaves and soil, which inhibited water absorption and electron transport and it caused the reduction of height growth rate more than 50 %. Although there was a little difference according to time and $CaCl_2$ concentration, all treatments decreased in growth rate and physiological activity slowed down. As time passed, these results got worse. Therefore we need to take a measure earlier in order to minimize damage of trees.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.354-362
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• 2009

This study was performed to develop a suitable nutrient solution for standard rose substrate culture in a closed hydroponic system. 1/4, 1/2, 2/3 and 1 strength of the nutrient solution made by Japan National Institute of Vegetable and Tea Science (JNIVT) were supplied. The photosynthesis rate, quality and growth of cut flower were higher in the 1/2 and 2/3 strength of nutrient solution during high and low temperature period. Based on the above results, optimum nutrient solutions (UOS) were composed by nutrientwater (n/w) absorption ratio with 1/2S ($NO_{3^-}N$ 6.8, $NH_{4^-}N$ 0.7, $PO_{4^-}P$ 2.0, K 3.8, Ca 3.0, Mg 1.2, $SO_{4^-}S$ $1.2me{\cdot}L^{-1}$) at high temperature season and 2/3($NO_{3^-}N$ 9.7, $NH_{4^-}N$ 0.8, $PO_{4^-}P$ 2.2, K 5.0, Ca 3.9, Mg 1.5, $SO_{4^-}S$ $1.5me{\cdot}L^{-1}$) at low temperature season. The results of suitability examination showed that the EC level in newly composed nutrient solution (UOS) was more stable than other nutrient solutions due to its large amount of calcium and potassium. The growth of cut flower cultivated with UOS was higher than those of other nutrient solutions. Especially, the yield of cut flowers in UOS nutrient solution increased 1.4 times than that of other nutrient solution treatments. Consequently, the new nutrient solution investigated in this experiment was suitable for rose cultivation in a closed hydroponic system.

• Journal of Korean Society of Forest Science
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• v.106 no.3
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• pp.288-299
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• 2017

This study was conducted to find out the influence of drought stress on physiological responses of Synurus deltoides. Drought stress was induced by withholding water for 25 days. Leaf water potentials were decreased of both predawn (${\Psi}_{pd}$) and mid-day (${\Psi}_{mid}$) with increasing drought stress, but water saturation dificit (WSD) was 7 times increased. ${\Psi}_{pd}-{\Psi}_{mid}$ showed the significant difference of 0.22~0.18 MPa in stressed before 10 days, and nonsignificant as treatment time became longer. A strong reduction of stomatal conductance ($gH_2O$) and stomatal transpiration rate (E) were observed after 15 days of drought stress Significant reductions of net apparent quantum yield (${\Phi}$) and maximum photosynthesis rate ($Pn_{max}$) were observed after 20 days of drought stress; However, water use efficiency (WUE) was shown the opposite trend. This implies that decrease of photosynthesis rate may be due to an inability to regulate water and $CO_2$ exchanged through the stomata. From JIP analysis, flux ratios (${\Psi}_O$ and ${\Phi}_{EO}$) and performance index on absorption basis ($PI_{ABS}$) were dramatically decreased withholding water after 15 days, which reflects the relative reduction of photosystem II activity. The leaf of S. deltoides showed osmotic adjustment of -0.35 MPa at full turgor and -0.40 MPa at zero turgor, and also cell-wall elastic adjustment of 9.4 MPa, indicating that S. deltoides tolerate drought stress through osmotic adjustment and cell-wall elastic adjustment. The degree of change in water relations parameters such as Vo/DW, Vt/DW decreased with increasing drought stress. This result showed that S. deltoides was exhibited a strong reduction of photosynthetic activity to approximately -0.93 MPa of predawn leaf water potential, and both of osmotic adjustment and cell-wall elastic adjustment in drought stress condition appears to be an important adaptation for restoration in this species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.3
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• pp.201-210
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• 1990

This study was carried out to determine the effect of mixture of hymexazole and metalaxyl(Tachigarace) on the growth and reducing low temperature injury in rice seedlings. The amount of mixture of hymexazole and metalaxyl (Tachigarace) applied to reduce low temperature injury was 0, 9, 18 and 36mgr per pot (5${\times}$15${\times}$10cm) by soil incorperation befor sowing. Aging seedling was investigated on the growth responses by the application of 0.36gr mixture of hymexazole and metalaxyl (Tachigarace) per tray (30${\times}$60${\times}$3cm) after cutting of leaf at 35 day-old seedling. Rice seedlings treated with mixture of hymexazole and metalaxyl (Tachigarace) were better in the growth of top and roots, dry weight, erecting and rooting abilities than untreated seedlings. The effective amount of mixture of hymexazole and metalaxyl (Tachigarace) to increase erecting and rooting abilities was 18 mgr per pot. Under low temperature treatment, 12$^{\circ}C$, the leaf discoloration and dead leaf percent of rice seedlings were markedly decreased in the seedlings treated with mixture of hymexazole and metalaxyl (Tachigarace) as compared with untreated seedlings. The more amount of mixture of hymexazole and metalaxyl (Tachigarace) upto 36mgr per pot, the more effective on the low temperature injury in the seedling. The effect of mixture of hymexazole and metalaxyl (Tachigarace) to reduce low temperature damage in rice seedlings was mainly due to decreasing less physiological activities on the expanding leaf area and rooting, transpiration, stomatal diffusion and chlorophyll content of the leaves and oxygen consumption of the roots. Plant height, top dry weight and leaf area of aging seedlings were increased with mixture of hymexazole and metalaxyl (Tachigarace) treatment and leaf cutting as compared with untreated seedling and intact leaf, respectively. The ratio of missing hill of aging seedling was increased with increasing the period of growing seedling and was decreased due to leaf cutting, whereas the effect of mixture of hymexazole and metalaxyl (Tachigarace) application was not observed. Rooting ability of aging seedlings was decreased with lengthening of the period of raised seedling and was increased with mixture of hymexazole and metalaxyl (Tachigarace) treatment and leaf cutting.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.311-316
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• 2011

This study aimed to determine the effect of EC (electrical conductivity) levels of nutrient solution in hydroponic culture on allyl-isothiocyanate (AITC) content within plant tissues, Vitamin C content and physiological responses in wasabi plant (Wasabia japonica M. 'Darma'). The 'Darma' was grown for 5 weeks with a deep flow technique (DFT) system controlled at 5 different EC levels, including 0.5, 1, 2, 3, and $5dS{\cdot}m^{-1}$. In result, the highest total content of AITC showed at EC level 5 and $3dS{\cdot}m^{-1}$ for 1 or 5- week, respectively. The total content of AITC increased about 1.2-1.4 times when the plants were grown in the EC levels between 0.5 and $2dS{\cdot}m^{-1}$, whereas the content decreased about 6 and 56 % in the EC level 3 and $5dS{\cdot}m^{-1}$, respectively. The content of AITC was relatively higher in petiole tissue, about 53 %, taken from 1 week-grown plants when the EC was controlled between 0.5 and $2dS{\cdot}m^{-1}$. Root tissue also had relatively higher content of AITC, about 45.1 %, when the EC was controlled at 3 and $5dS{\cdot}m^{-1}$. However, a 5-fold decrease in the AITC content was found in blade tissue and a 6.8-fold decrease in root when the EC was controlled at $5dS{\cdot}m^{-1}$ for 5 weeks. There was no significant difference in the vitamin C content in 1-week grown leaf tissues under the different EC level treatments; but, the content increased about 27% in 5-week grown plants at the EC level between 0.5 and $2dS{\cdot}m^{-1}$, compared to the 1 week-grown leaf tissue. Electrolyte leakage of leaf tissue taken from 3-week grown plant was 3-fold higher at the EC level $5dS{\cdot}m^{-1}$, compared to the EC level between 0.5 and $2dS{\cdot}m^{-1}$. Chlorophyll content, photosynthesis rate and transpiration rate were decreased when the EC was controlled at higher than $2dS{\cdot}m^{-1}$. Leaf water content, specific leaf area and growth were decreased when the EC was controlled at $5dS{\cdot}m^{-1}$ for 5 weeks. All the integrated results in this study suggest that the EC level of nutrient solution should be maintained at lower than $3dS{\cdot}m^{-1}$ in order to improve nutritional value and quantity required for hydroponically grown wasabi as functional vegetable.