• Title/Summary/Keyword: transgenic embryo

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Analysis of human HoxA gene control region and its effects on anterior-posterior axial pattern formation using transgenic mouse embryo (Transgenic mouse embryo를 이용한 human HoxA 유전자의 조절부위 분석과 전후축 형태형성(anterior-posterior axial pattern formation)에 미치는 영향)

  • Jang, Seung-ik;Min, Won-gi;Park, Jong-hoon;Lee, Chul-sang;Lee, Kyung-kwang;Lee, Young-won;Jun, Moo-hyung;Kim, Myoung-hee
    • Korean Journal of Veterinary Research
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    • v.35 no.1
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    • pp.95-105
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    • 1995

  • The human homolog of position specific element of mouse Hoxa-7 was studied using transgene. It contains a 1.1 kb human DNA (HCR)- a homolog to the intergenic region between Hoxa-7 and -9, which directs the position specific expression of Hoxa-7-, tk promoter, LacZ (${\beta}$-galactosidase) gene as a reporter, and polyadenylation signal of SV40 large T antigen. It was injected into the mice embryos, and the resulting transgenic embryos were analysed through PCR as well as genomic Southern blotting with placenta DNA. Out of 20 embryos analysed, two were transgenic. Among them, one transgenic embryo expressed transgene when stained with X-gal. The expression pattern was in analogy to that of the mouse Hoxa-7, showing spatially restricted expression pattern, Since the expression of ${\beta}$-galactosidase is regulated by the upstream human HCR sequence, it implies that the HCR is the plausible position specific regulatory element of human.

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Preselection and cloning of transgenic emb (유전자전환 수정란의 선별과 복제)

  • Lee, Hyo-Jong
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 1998.05a
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    • pp.12-28
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    • 1998

  • The technology of creating transgenic animals has a potential value in improving productivity and disease resistance of animals, gene therapy, drug pharming and production of model animals for certain diseases. Up to date, fairly low success rate of production of transgenic animals and a pronounced variability with respect to the expression of transgenes have been much observed. The mechanisms how to integrate the injected genes with a certain part of the genomes are unknown yet. Many techniques in gene transfer, beside microinjection, have been introduced and explored thus to improve the production efficiency of transgenic animals. In this article, the methods and efficiency of gene-transfer techniques, the detection and preselection of transgenes in embryos by PCR- and GFP-screenings and cloning of preselected transgenic embryos by nuclear transplantation are described and discussed. Some experimental results showed that the early screening and selection of integration of the injected gene with embryonic genome by polymerase chain reaction(PCR) and green fluorecence protein(GFP) were promising methods. Further, the application of nuclear transplantation technology to cloning and multiplication of the positively integrated genes in the cleaving embryos and embryonic cells will be beneficially used for the mass production of transgenic embryos and consequently improving the production efficiency in transgenic animals.

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