• Title/Summary/Keyword: transformation region

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Transformation of Medicago truncatula with rip1-GUS Gene

  • Nam Young-Woo;Song Dae-Hae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.5
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    • pp.434-439
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    • 2004
  • Medicago truncatula is a model plant for molecular genetic studies of legumes and plant-microbe interactions. To accelerate finding of genes that play roles in the early stages of nodulation and stress responses, a trans-genic plant was developed that contains a promoter­reporter fusion. The promoter of rip], a Rhizobium-induced peroxidase gene, was fused to the coding region of $\beta-glucuronidase (GUS)$ gene and inserted into a modified plant transformation vector, pSLJ525YN, in which the bar gene was preserved from the original plasmid but the neomycin phosphotransferase gene was replaced by a polylinker. Transformation of M. truncatula was carried out by vacuum infiltration of young seedlings with Agrobacterium. Despite low survival rates of infiltrated seedlings, three independent transformants were obtained from repeated experiments. Southern blot analyses revealed that 7 of 8 transgenic plants of the T 1 generation contained the bar gene whereas 6 $T_1$ plants contained the GUS gene. These results indicate that vacuum infiltration is an effective method for transformation of M. truncatula. The progeny seeds of the transgenic plants will be useful for mutagenesis and identification of genes that are placed upstream and may influence the expression of rip] in cellular signaling processes including nodulation.

Numerical Simulation of Longshore Current due to Random Sea Waves (불규칙파에 의한 연안류의 수치계산)

  • 권정곤;양윤모
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.4 no.2
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    • pp.72-82
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    • 1992
  • To accurately estimate nearshore current in shallow water regions. it is necessary to investigate the irregular wave transformation characteristics and radiation stress produced by random sea waves. This research is to investigate the application or the individual wave Analysis Method. the Component Wave Analysis Method and Representative Wave Analysis Method in the shallow water region. These methods were estimated by wave shallowing transformation when the waves propagate from deep water to shallow water region b)r generating regular waves, two component waves and irregular waves (Bretschneider-Mitsuyasu type). That is, the Indivisual Wave Analysis Method is to investigate from the viewpoint of shallow water transformation of wave statistical characteristics and their zero-down-crossing waves (wave height period and wave celerity). And the component Wave Analysis Method is to investigate from the view point of shallow water transformation of basic frequency component wave and their interference frequency component wave. In addition, this research is to compare the measured mean water level elevation with the calculated one from radiation stress of irreguar waves that is assumed in the three methods above.

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Phase Transformation and Luminescent Properties of Ca1-xSrxAl2O4:Eu2+ Phosphors ([Ca1-xSrxAl2O4:Eu2+] 형광체의 상전이 및 발광특성에 관한 연구)

  • Park, Yun-Jin;Song, Hyun-Don;Jung, Sang-Hyun;Lee, Jee-Hee;Hwang, Min-Ha;Kim, Young-Jin
    • Korean Journal of Materials Research
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    • v.18 no.1
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    • pp.1-4
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    • 2008
  • The phase transformations and luminescent properties of Eu-doped $Ca_{1-x}Sr_xAl_2O_4$ phosphors were investigated. $Ca_{1-x}Sr_xAl_2O_4:Eu^{2+}$ phosphors were synthesized by a solid-state reaction with a flux, $H_3BO_3$. A phase transformation from monoclinic $CaAl_2O_4$ to monoclinic $SrAl_2O_4$ was observed as the x values increased. A high-temperature hexagonal phase of $SrAl_2O_4$ was formed during this transformation as an intermediate phase under an $H_2$ atmosphere due to oxygen vacancies; this did not occur in an air atmosphere. Accordingly, the emission spectra shifted from a blue region to a green region as x increased.

The Development of Air Quality Model Considering Shipping Source in Pusan Region (선박배출 오염물질의 영향을 고려한 부산지역 대기질 모델의 개발)

  • 이화운;김유근;원경미
    • Journal of Environmental Science International
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    • v.8 no.1
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    • pp.135-144
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    • 1999
  • Air quality modeling about coastal urban region such as Pusan shoud be consider shipping source emmited from ships anchoraging and running. It has been proved at our previous studies that the ratios of air pollutants emission amount in coastal area to inland are 12.2% for NO$_2$ and 11.7% for $SO_2$ and the air qualify of coastal urban area consierably counts on ships. Also the dispersion pattern of the all pollutants followed local circulation system in this region. Therefore this study has been developed air quality model which can describe the formation, transport, transformation and deposition processes of air pollutants considering shipping source. Currently, restriction for emission amount of ships does not exist, so our study will be useful to set the omission standard and for devising air quality policy in coastal urban region.

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Residual Stress Analysis of Hot Rolled Strip (열연 강판의 잔류 응력 해석)

  • 구진모;김홍준;이재권;황상무
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2003.05a
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    • pp.172-175
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    • 2003
  • Run-Out-Table is the region between EDT and CT. Hot killed strip is cooled by air and water in ROT. In this procedure, phase transformation and shape deformation occur due to temperature drop. Because of un-ideal cooling condition, deformation of strip and non-uniform phase distribution come into existence. This phenomenon affects the strip property and lead th the existence of residual stress. And it exerts effects on the Coiling process, Coil Cooling process, and Un-coiling process. Through these process, the residual stresses of strip are more larger and unbalance of these stresses become more severe. Finite element (FE) based models for the analysises of non-steady state heat transfer and elastoplastic deformation are described in this investigation. The analysises of thermodynamics and phase transformation kinetics are suggested also. Using the ROT simulation result coiling process and coil cooling process simulations are carried out.

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Recognition of Car Manufacturers using Faster R-CNN and Perspective Transformation

  • Ansari, Israfil;Lee, Yeunghak;Jeong, Yunju;Shim, Jaechang
    • Journal of Korea Multimedia Society
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    • v.21 no.8
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    • pp.888-896
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    • 2018
  • In this paper, we report detection and recognition of vehicle logo from images captured from street CCTV. Image data includes both the front and rear view of the vehicles. The proposed method is a two-step process which combines image preprocessing and faster region-based convolutional neural network (R-CNN) for logo recognition. Without preprocessing, faster R-CNN accuracy is high only if the image quality is good. The proposed system is focusing on street CCTV camera where image quality is different from a front facing camera. Using perspective transformation the top view images are transformed into front view images. In this system, the detection and accuracy are much higher as compared to the existing algorithm. As a result of the experiment, on day data the detection and recognition rate is improved by 2% and night data, detection rate improved by 14%.

Vehicle Manufacturer Recognition using Deep Learning and Perspective Transformation

  • Ansari, Israfil;Shim, Jaechang
    • Journal of Multimedia Information System
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    • v.6 no.4
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    • pp.235-238
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    • 2019
  • In real world object detection is an active research topic for understanding different objects from images. There are different models presented in past and had significant results. In this paper we are presenting vehicle logo detection using previous object detection models such as You only look once (YOLO) and Faster Region-based CNN (F-RCNN). Both the front and rear view of the vehicles were used for training and testing the proposed method. Along with deep learning an image pre-processing algorithm called perspective transformation is proposed for all the test images. Using perspective transformation, the top view images were transformed into front view images. This algorithm has higher detection rate as compared to raw images. Furthermore, YOLO model has better result as compare to F-RCNN model.

Dynamic characteristics analysis of forcing jet by Karhunen-Loeve transformation (Karhunen-Loeve 변환을 이용한 Forcing 제트의 동적 특성 해석)

  • Lee, Chan-Hui;Lee, Sang-Hwan
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.21 no.6
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    • pp.758-772
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    • 1997
  • The snapshot method is introduced to approximate the coherent structures of planar forcing jet flow. The numerical simulation of flow field is simulated by discrete vortex method. With snapshot method we could treat the data efficiently and approximate coherent structures inhered in the planer jet flow. By forcing the jet at a sufficient amplitude and at a well-chosen frequency, the paring can be controlled in the region of the jet. Finally we expressed the underlying coherent structures of planar jet flow in the minimum number of modes by Karhunen-Loeve transformation in order to understand jet flow and to make the information storage and management in computers easier.

Effects of V on the Formation of Ti-Nb-V Cabonitrides and Mechanical Properties in Low Carbon HSLA Steels (저탄소.저합금강의 Ti-Nb-V 복합 탄질화물 형성 및 기계적 특성에 미치는 V 첨가의 효과)

  • Kang, J.S.;Kim, D.J.;Park, C.G.
    • Transactions of Materials Processing
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    • v.15 no.8 s.89
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    • pp.581-585
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    • 2006
  • Effects of V on both the formation of Ti-Nb-V carbonitrides and mechanical properties of Ti-Nb bearing low carbon HSLA steels were investigated. Hot rolling process was simulated by using Gleeble 3500 system with the steels containing three different levels of V ($0{\sim}0.1wt.%$). Vanadium precipitated as Ti-Nb-V carbonitrides at austenite region but it did not precipitate as VC during austenite to acicular ferrite or bainitic ferrite phase transformation. As V content increased, the amount of Nb precipitates was decreased but the average size of Ti-Nb-V carbonitrides was increased due to larger diffusivity of V than that of Nb. Coarsened Ti-Nb-V carbonitrides could act as heterogeneous nucleation site during ${\gamma}{\rightarrow}{\alpha}$ phase transformation, thus, acicular ferrite transformation was promoted as V content increased, resulting in increase of upper shelf energy.

Entrepreneurial Learning and Indian Tech Startup Survival: An Empirical Investigation

  • Krishna, HS
    • Asian Journal of Innovation and Policy
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    • v.7 no.1
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    • pp.55-78
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    • 2018
  • This paper investigates the linkage between the mode of transformation of entrepreneurial learning into outcomes and the subsequent impact of these learning outcomes in enhancing the survival of high-tech startups in India. The study uses data from 45 high-tech startups headquartered across different locations in India for the purpose of analysis. Survival Analysis of the data is conducted to determine which mode of learning transformation and what type of en trepreneurial decision making preference have a significant influence on the survival of Indian high-tech startups and to what extent do they impact their survival. The results indicate that entrepreneur's prior startup experience, explorative mode of learning transformation, causal decision making of the entrepreneur and availability of funding for the startup as the key factors that reduce the time to survival of Indian high-tech startups. They also provide key insights on how these factors impact the startup survival in this region.