• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.12 no.5
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• pp.323-329
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• 2002

This paper presents the application of the technique of differential transformation to the post-buckling problem of non-uniform cantilever column subjected to a combined load. Numerical calculations are carried out and compared with previously published results to validate the results of the present method. The results obtained by this method agree very well with those reported in the previous works. The results obtained by the present method are presented for both various non-uniform columns and loads.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.16 no.1
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• pp.10-17
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• 2004

In this paper, the affine transformation method that is more simpler compare with digital orthophoto method is used analyzed the long-term shoreline change, and accuracy estimation was carried out. As a result of this study, it was able to check that the shoreline change on Namhangjin coast had eroded significantly compare with the past. Moreover, as a result of accuracy estimation, it shows that the RMS error around shoreline was about 1-2 m. In consideration that maximum allowable error shown in aerial photogrammetry specification is within 2 m, therefore, analysis results of shoreline change using affine transformation method on aerial images is reliable.

• Proceedings of the Korea Institute of Convergence Signal Processing
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• 2000.08a
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• pp.269-272
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• 2000

It is need to develop and apply a human pulse diagnosis system providing a quantitative and automatic analysis in the the oriental medicine. In order to analyze quantitatively the characteristic of pulsation, each of points had to be recognized accurately notifying the existence and the position of feature point in the wave form. And getting the period of human pulse. Thus, in this paper, it is proposed the preprocessing method of human pulse and the detection method of period by Wavelet Transformation. The human pulse is seprated from each band through Wavelet Transformation and feature points can be recognized through over the fact, and then the parameter of proposed Mac-Jin parameter is measured. Commonly, Human pulse signal has often various noises which are baseline drift, high frequency noise and so on. So it is significant to remove that noises. Thus, in this paper, the one period of human pulse is deciede and the feature points are detected after doing the preprocessing by wavelet transformation. As a result, it could be confirmed that this method is effective as a real program for the auto-diagnosis of human pulse.

• Clean Technology
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• v.21 no.2
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• pp.90-95
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• 2015

Digital microfluidic electroporation system was used for the transformation of microalgae and we have obtained higher transformation efficiency and viability than that of conventional method. Key parameters of electroporation such as pulse voltage, number, and duration time were systematically investigated for two different microalgal strains with and without cell wall. We have found that cell wall does not always have negative effects on the gene transformation of microalgae. Parallel processing of proposed digital microfluidic electroporation was demonstrated together with on chip culture of microalgae.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.698-701
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• 2005

This paper examines that is extracted certain information in forest areas within high resolution imagery based on wavelet transformation. First of all, study areas are selected one more species distributed spots refer to forest type map. Next, study area is cut 256 x 256 pixels size because of image processing problem in large volume data. Prior to wavelet transformation, five texture parameters (contrast, dissimilarity, entropy, homogeneity, Angular Second Moment (ASM≫ calculated by using Gray Level Co-occurrence Matrix (GLCM). Five texture images are set that shifting window size is 3x3, distance .is 1 pixel, and angle is 45 degrees used. Wavelet function is selected Daubechies 4 wavelet basis functions. Result is summarized 3 points; First, Wavelet transformation images derived from contrast, dissimilarity (texture parameters) have on effect on edge elements detection and will have probability used forest road detection. Second, Wavelet fusion images derived from texture parameters and original image can apply to forest area classification because of clustering in Homogeneous forest type structure. Third, for grading evaluation in forest fire damaged area, if data fusion of established classification method, GLCM texture extraction concept and wavelet transformation technique effectively applied forest areas (also other areas), will obtain high accuracy result.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.2
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• pp.193-200
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• 2013

In this paper, underwater acoustic image classification of a cylindrical object using the Hough transformation is proposed. Hough transformation is often used to classify a cylindrical object in the optical systems. However, it is difficult to apply to the underwater acoustic image system because of lower resolution and noisier underwater environments. Thus, the cylindrical object was modeled and its geometric depth(GD) pixels were restored in order to make them suitable for the Hough transformation by using moving average filter and a polynomial interpolation method. As a result, restored GD pixels are similar to original ones and test results show high performance in classification.

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.69-75
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• 2004

Optimal protocol for efficient genetic transformation has been defined to aid future strategies of genetic engineering in pigeon pea with agronomically important genes. Transgenic pigeonpea plants were successfully produced through Agrobacterium tumefaciens-mediated genetic transformation method using cotyledonary node explants by employing defined culture media. The explants were co-cultivated with A. tumefaciens strain C-58 harboring the binary plasmid, pCAMBIA-1301 [con-ferring $\beta$-glucuronidase(GUS) activity and resistance to hygromycin] and cultured on selection medium (regeneration medium supplemented with hygromycin) to select putatively transformed shoots. The shoots were then rooted on root induction medium and transferred to pots containing sand and soil mixture in the ratio of 1:1. About 22 putative TO transgenic plants have been produced. Stable expression and integration of the transgenes in the putative transgenics were confirmed by GUS assay, PCR and Southern blot hybridization with a transformation efficiency of over 45%. Stable integration and expression of the marker gene has been confirmed in the TO and T1 transgenics through PCR, and Southern hybridization.

• Structural Monitoring and Maintenance
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• v.4 no.1
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• pp.33-52
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• 2017

A cantilever beam with a breathing crack is studied to improve the breathing crack identification sensitivity by the parametric optimization of sample entropy and wavelet transformation. Crack breathing is a special bi-linear phenomenon experienced by fatigue cracks which are under dynamic loadings. Entropy is a measure, which can quantify the complexity or irregularity in system dynamics, and hence employed to quantify the bi-linearity/irregularity of the vibration response, which is induced by the breathing phenomenon of a fatigue crack. To improve the sensitivity of entropy measurement for crack identification, wavelet transformation is merged with entropy. The crack identification is studied under different sinusoidal excitation frequencies of the cantilever beam. It is found that, for the excitation frequencies close to the first modal frequency of the beam structure, the method is capable of detecting only 22% of the crack depth percentage ratio with respect to the thickness of the beam. Using parametric optimization of sample entropy and wavelet transformation, this crack identification sensitivity is improved up to 8%. The experimental studies are carried out, and experimental results successfully validate the numerical parametric optimization process.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.5
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• pp.434-439
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• 2004

Medicago truncatula is a model plant for molecular genetic studies of legumes and plant-microbe interactions. To accelerate finding of genes that play roles in the early stages of nodulation and stress responses, a trans-genic plant was developed that contains a promoter­reporter fusion. The promoter of rip], a Rhizobium-induced peroxidase gene, was fused to the coding region of $\beta-glucuronidase (GUS)$ gene and inserted into a modified plant transformation vector, pSLJ525YN, in which the bar gene was preserved from the original plasmid but the neomycin phosphotransferase gene was replaced by a polylinker. Transformation of M. truncatula was carried out by vacuum infiltration of young seedlings with Agrobacterium. Despite low survival rates of infiltrated seedlings, three independent transformants were obtained from repeated experiments. Southern blot analyses revealed that 7 of 8 transgenic plants of the T 1 generation contained the bar gene whereas 6 $T_1$ plants contained the GUS gene. These results indicate that vacuum infiltration is an effective method for transformation of M. truncatula. The progeny seeds of the transgenic plants will be useful for mutagenesis and identification of genes that are placed upstream and may influence the expression of rip] in cellular signaling processes including nodulation.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.323-330
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• 2019

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.