• 제목/요약/키워드: transaminase

검색결과 820건 처리시간 0.027초

連續的 溫熱曝露가 흰쥐의 血淸 Glutamic Oxaloacetic Transaminase 및 Glutamic Pyruvic Transaminase의 活性에 미치는 影響 (Effect of Prolonged Heat Exposure on Serum Glutamic Oxaloacetic and Glutamic Pyruvic Transaminase Activities of Rats)

  • Park, Yun-Kwun;Nam, Sang-Yul
    • 한국동물학회지
    • /
    • 제17권3호
    • /
    • pp.131-138
    • /
    • 1974
  • 成熟한 Sprague-Dawley 系의 雄性흰쥐를 $30\\pm 0.5^\\circ C$에서 240時間 그리고 $33\\pm 0.5^\\circ C$에서 64時間 各各 溫熱曝露시켰으며, 溫熱曝露 期間中 여러 時間區에서 血淸 glutamic oxaloacetic transaminase (GOT)와 血淸 glutamic pyruvic transaminase (GPT)의 活性을 Reitman 및 Frankel (1957)의 方法에 依해서 Coleman-Model 295E Spectrophotometer로서 各各 550m$\\mu$에서 測定하였다. $23\\pm 1^\\circ C$인 對照群에 比較하여 보면, $30\\pm 0.5^\\circ C$ 및 $33\\pm 0.5^\\circ C$에서 溫熱曝露된 흰쥐는 各各 血淸의 GOT 및 GPT의 活性이 有意性있게 上昇되었다. 서로 다른 高溫의 溫熱에 曝露된 흰쥐의 血淸 GOT 活性은 16 및 30時間 區間中에서 또한 64 및 72時間 區間中에서 各各 有意性있게 上昇되었다. 한편 $30\\pm 0.5^\\circ C$에서 72時間後에는 血淸 GOT 活性은 正常群으로 되돌아가는 傾向이 나타났다. 서로 다른 高熱의 溫熱로 曝露된 흰쥐의 血淸GPTdml 活性은 初期인 4時間區에서 有意性있게 上昇되었으며, 이어서 16時間區와 64 및 72時間 區間中에서 各各 上昇되었다. 한편 $30\\pm 0.5^\\circ C$에서는 114時間後에 약간 增加하는 傾向이 나타났다. $30\\pm 0.5^\\circ C$와 $33\\pm 0.5^\\circ C$에서 各各 溫熱曝露된 흰쥐의 酵素活性을 比較하여 보면, $33\\pm 0.5^\\circ C$에 曝露된 흰쥐의 血淸 GOT 및 血淸 GPT의 活性은 各各 16, 30 및 64時間區에서 有意性있게 높은 活性을 나타내었다. 이러한 結果로 미루어 보아 連續的 溫熱曝露는 非正常의 아미노基 轉移代謝를 招來하는 것으로 思料된다.

  • PDF

Pentazocine의 가토혈당(家兎血糖), 혈청(血淸) Transaminase 및 Alkaline Phosphatase 활성도(活性度)에 대(對)한 작용(作用) (The Action of Pentazocine on the Blood Sugar Level, Serum Transaminase and Alkaline Phosphatase Activities in Rabbits)

  • 박정인;이운구;홍권희;김양순
    • 대한약리학회지
    • /
    • 제9권1호
    • /
    • pp.47-51
    • /
    • 1973
  • A benzomorphan derivative, pentazocine has both opioid agonistic actions and weak narcotic antagonistic activity. In this paper, authors attempted to study the dose response of pentazocine on the blood sugar level, serum transaminase and alkaline phosphatase activity in rabbits. Eighteen rabbits were devided into 3 groups, and each group were injected with pentazocine 5mg, 15mg, and 30mg/kg respectively. And metabolic effects were investigated measuring the change of blood sugar contents, serum transaminase (S-GOT, S-GPT) activities and alkaline phosphatase activities in rabbits. The results were obtained as follows; 1. Pentazocine significantly increased the blood sugar content in rabbits. 2. Pentazocine significantly increased the serum GOT activity but in the serum GPT activity, it significantly increased in large dose. 3. Pentazocine significantly increased serum alkaline phosphatase activity.

  • PDF

6-Aminonicotinamide가 토끼혈청내 효소 및 대사물질에 미치는 영향 (Effect of 6-Aminonicotinamide on the Levels of Some Metabolites and Related Enzymes in Rabbit Serum)

  • 박인국;이철승;이승훈;송윤경;신숙
    • 한국동물학회지
    • /
    • 제33권4호
    • /
    • pp.493-498
    • /
    • 1990
  • Antimetabolited인 6-aminonicotinamide(6-AN)가 토끼의 혈청내 효소 및 대사 물질에 미치는 영향에 관하여 연구하였다. 복강투여시 (l5mg/kg의 체중) 포도당과 콜레스테놀농도는 현저이 증가하였으나 알부민고 총단백질량은 크게 변하지 않았다. Creatine phophokinase,glutamic oxaloacetate transaminase,glutamic pyruvate transaminase 및 alctate dehydrogensenase활성은 매우 증가하였으며 alkaline phosphatase와 Ca, P, Na, K, Cl 및 Co등의 수준은 영향을 받지 않았다.

  • PDF

오미자를 이용한 스포츠 드링크 섭취가 혈액성분과 운동수행력 및 HSP70에 미치는 영향 (The Effect of Sports Drink Containing Schizandra Chinensis on Blood Biochemical Elements, Exercise Performance and HSP70)

  • 오재근;김복주;신영오;정희정
    • 대한한의학회지
    • /
    • 제23권2호
    • /
    • pp.139-150
    • /
    • 2002
  • OBJECTIVES : Schizandra chinensis is well known for its efficacy at liver function reinforcement, relieving thirst and recovery from fatigue. In this study, we examined the effects of sports drink containing Schizandra chinensis on serum metabolic substrate, electrolyte, stress indicators, related-enzyme and exercise performance, rectal temperature, and heat shock proteinb70 (HSP70). METHODS : Elite long-distance runners (male, 21.3yrs, n=16) were selected and divided into two groups; an experimental group (EXP, n=8) and a control group (CON, n=8). A beverage containing Schizandra chinensis was supplemented 3 times per day to EXP for 4 weeks. Serum biochemical elements (glucose, lactate, total cholesterol, triglyceride, high density lipoprotein cholesterol, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, creatinine, creatine phosphokinase, lactate dehydrogenase, blood urea nitrogen, Na, K, Cl) were analyzed by auto blood analyzer. Exercise performance was measured by treadmill exercise test, HSP70 was detected by electrophoresis and Western blotting, and rectal temperature was measured by rectal temperature probe. RESULTS : Administration of the beverage increased significantly the rest level of blood Na, Cl and glucose and decreased significantly lactate dehydrogenase, glutamate oxaloacetate transaminase. No difference was found in exercise performance, rectal temperature increment or HSP70 concentration between groups. CONCLUSIONS : Administration of a sports drink containing Schizandra chinensis altered blood glucose, lactate dehydrogenase, glutamate oxaloacetate transaminase, Na and Cl levels.

  • PDF

Hepatoprotective Activity of Thespesia populnea Bark Extracts against Carbon Tetrachloride-Induced Liver Toxicity in Rats

  • Ilavarasan, R.;Vasudevan, M.;Anbazhagan, S.;Venkataraman, S.;Sridhar, S.K.
    • Natural Product Sciences
    • /
    • 제9권2호
    • /
    • pp.83-86
    • /
    • 2003
  • In the present study, Thespesia populnea (Malvaceae) bark was extracted with methanol and water. The extracts were vacuum dried to yield the respective methanol (MET) and aqueous extract (AET). The extracts were evaluated for hepatoprotective activity against carbon tetrachloride $(CCl_{4})$ induced liver damage at 2 dose levels (250 and 500 mg/kg). The biochemical parameters observed in serum were total bilirubin, alkaline phosphatase (ALP), serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) levels and total protein. Aspartate transaminase (AST), alanine transaminase (ALT) and total protein levels in liver were also evaluated. Histopathological study on the liver tissue was also performed. The extracts exhibited dose dependent reduction in total bilirubin, ALP SGOT, SGPT, AST, ALT and increase in total protein (serum and liver) levels. The extracts also exhibited only mild hepatocytic damage compared to the $CCl_{4}$ Treated group. MET was found to exhibit higher hepatoprotection than AET.

홍경천의 과도한 운동스트레스에 대한 방어효과 (Protective Effect of Rhodiola sachalinensis A. Bor on Excessive Exercise Stress)

  • 이연미;홍희도;최상윤
    • Journal of Applied Biological Chemistry
    • /
    • 제56권1호
    • /
    • pp.1-3
    • /
    • 2013
  • 본 연구결과에서 홍경천 추출물은 L6 근육세포에서 산화적 손상으로 인한 세포사멸과 ATP 생성량 감소를 유의적으로 억제하였을 뿐만 아니라 마우스에 2주간 200 mg/kg 농도로 경구투여시 통계적으로 유의하지는 않았으나 treadmill 운동시간을 증가시켰고 glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, lactate dehydrogenase, creatinine, lactate 등의 혈액지표를 개선시켰다. 따라서 홍경천은 과도한 운동 스트레스로 인한 손상을 억제할 수 있는 소재로써의 큰 가능성을 가지고 있는 것으로 판단된다.

상엽과 산사엽 추출물이 사염화 탄소로 유발된 흰쥐의 간 손상에 미치는 영향 (Effects of Mori Folium and Crataegus pinnatifida Leave Extracts on $CC1_4$-induced Hepatotoxicity in Rats)

  • 김형진;김중권;황완균;함인혜;권석형;황보식;김홍진
    • 약학회지
    • /
    • 제47권4호
    • /
    • pp.206-211
    • /
    • 2003
  • This study was performed to investigate the effect of Mori Folium and Crataegus pinnatifida leave extracts on liver damage induced by CC1$_4$ in rats. 30% and 60% methanol extracts of Mori Folium and Crataegus pinnatifida leaves were administered orally at the dose of 10 mg/kg on every day for 6 days and liver damage was induced by intraperitoneal injection of $CCl_4$ (0.4 ml/kg) on 6th day. 30% Methanol extracts of Mori Folium treated group showed significant protective effect on hepatotoxicity with the lowest Glutamic Oxaloacetic Transaminase (GOT) and Glutamic Pyruvic Transaminase (GPT). The low density lipoprotein (LDL) levels of 30% methanol extracts of Mori Folium and Crataegus pinnatifida leave and 60% methanol extracts of Mori Folium treated groups were significantly lower than normal group, respectively. In particular, LDL level of 30% Mori Folium extracts treated group was significantly decreased compared to $CCl_4$ treated group. These results support that Mori Folium and Crataegus pinnatifida leave extracts are hepatoprotective effects against hepatotoxicity induced by $CCl_4$.

흰쥐에서 구속스트레스에 대한 우루소데옥시콜린산의 항스트레스 효과 (Anti-stress Effects of Ursodexycholic Acid on the Restraint Stress in Rats)

  • 조태순;이선미;염제호;유은주;임승욱;장병수;김영만;유영효;박명환
    • 약학회지
    • /
    • 제39권5호
    • /
    • pp.548-553
    • /
    • 1995
  • Effects of restraint stress and its modulations by ursodeoxycholic acid(UDCA) were evaluated on some biochemical and biophysical parameters in rats. Restraint stress induced elevations in blood alkaline phosphatase (ALP). cholesterol (CHOL), aspartate transaminase (GOT), alanine transaminase (GPT), lactate dehydrogenase (LDH) levels. It was also caused adrenal hypertrophy, decrease in weight of spleen and contents of ascorbic acid in stressed rats. As a results, stress indicators such as spleen, ascorbic acid, GOT, GPT, LDH were fastly changed after imposing stress, but those such as ALP, CHOL, adrenal were induced relatively later. UDCA was tested if it has an inhibitory effect against 18-hr restraint induced stress. UDCA lowered ALP, CHOL, LDH level and also effectively elevated the ascorbic acid contents in 25 mg/kg dosage of UDCA. In organ weights. the restraint stress induced increases in spleen and adrenal were attenuated by UDCA in 50 mg/kg dosage. However. stress-induced GOT and GPT levels were unaffected by UDCA.

  • PDF

복부 둔상 환자에게 간 손상 예측을 위한 Liver Transaminase의 유용성 (The Utility of Liver Transaminase as a Predictor of Liver Injury in Blunt Abdominal Trauma)

  • 이종석;오성찬;김혜진;조석진;이상래;류석용
    • Journal of Trauma and Injury
    • /
    • 제23권2호
    • /
    • pp.151-156
    • /
    • 2010
  • Purpose: The liver is the second most common organ injured by blunt abdominal trauma. The purpose of this study was to determine the utility of liver transaminase in screening blunt abdominal trauma patients for traumatic liver injury. Methods: We retrospectively reviewed the medical records of 231 patients who sustained blunt trauma and were at risk for traumatic liver injury between June 2009 and August 2010. All of them underwent a focused assessment with sonography for trauma (FAST) and abdominal computed tomography (CT). Based on the diagnosis of abdominal CT, patients were divided into two groups: group I with liver injury and group II without liver injury. We compared the two groups and calculated the sensitivity, the specificity and the predictive values of serum aspartate aminotransferase (AST) and serum alanine aminotransferase (ALT) by using multiple cutoff values. Results: Of 231 patients with no abdominal free fluid in the FAST, 33 had traumatic liver injury on abdominal CT. The mean AST and ALT levels in group I (311.6 IU/L and 228.1 IU/L, respectively) were significantly higher than the values in group II (48.4 IU/L and 35.6 IU/L, respectively). The cutoff to distinguish liver injury is 60 IU/L for AST and 58 IU/L for ALT, with 93.8% sensitivity and 79.8% specificity for AST, and 90.6% sensitivity and 87.4% specificity for ALT. Conclusion: We recommend that all patient with suspected blunt abdominal trauma be evaluated using serum liver transaminase as a screening test for liver injury even though no abdominal free fluid is shown on the FAST. If AST > 60 IU/L and/or ALT > 58 IU/L, abdominal CT was useful to confirm liver injury in this study.

G009가 Peroxidizers에 의해 유발된 지질 과산화에 미치는 영향 (Effect of G009 on Lipid Peroxidation Induced by Peroxidizer in Rats)

  • 이준우;정훈;이승목;김기남;한만덕;이승룡;김수웅;강상모
    • Biomolecules & Therapeutics
    • /
    • 제4권3호
    • /
    • pp.244-250
    • /
    • 1996
  • In this study, the anti-lipidperoxidative effects of G009, a polysaccharide extracted from Ganoderma lucidum IY009, was determined in ascorbic acid-$Fe^{2+}$-adenosine 5-diphosphate-intoxicated rat. In a model of ascorbic acid-Fe$^{2+}$-adenosine 5-diphosphate-induced hepatotoxicity in rat, G009 exhibited anti-lipidperoxidative effect in rat liver homogenate, and that malondialdehyde values of the liver homogenate inhibited from 48.1% to 74.8% in comparison to controls (p<0.05). The malondialdehyde formation in serum inhibited 66.5% at 100 mg/kg of G009. Also, serum levels of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in peroxidizer-induced rats treated with G009 was decreased compared with control. Especially, the formation of lipid peroxides in serum was related to glutamic pyruvic transaminase levels. These results suggest that G009 has a protective effect on ascorbic acid-$Fe^{2+}$-adenosine 5-diphosphate-induced hepatic injury through an inhibition of lipid peroxidation in liver.r.

  • PDF