• Asian-Australasian Journal of Animal Sciences
• /
• 제15권4호
• /
• pp.602-609
• /
• 2002

Lao-chao is a traditional Chinese fermented rice product with a sweet and fruity flavor, containing high levels of glucose, a little alcohol and milk-clotting characteristics. In order to optimize commercial production of lao-chao, Rhizopus javanicus and Saccharomyces cerevisiae were selected as the mold and yeast starter, respectively. A commercial mixed starter (chiu-yao) was used as control. Fermentation of the experimental combination revealed a sharp drop in pH (to 4.5) on the fourth day, remaining constant thereafter. Content of reducing sugars gradually decreased throughout the entire fermentation period. Of the free amino acids, higher quantities of alanine, leucine, proline, glutamic acid, glutamine and $NH_3$ were noted. For sugars, glucose revealed the highest concentration, while organic acid levels, including those for oxalic, lactic, citric and pyroglutamic acid, increased throughout the fermentation period. Twenty-one compounds were identified by gas chromatography from aroma concentrates of the lao-chao culture filtrate, prepared using the headspace method. For the flavor components, higher quantities of ethanol, fusel oil and ester were determined in both culture filtrates. In regard to the evaluation of yogurt-like product, there were significant differences in alcoholic smell, texture and curd firmness.

• 한국조리학회지
• /
• 제20권6호
• /
• pp.91-101
• /
• 2014

본 연구에서는 우리나라 고유의 전통발효유인 타락을 이용하여 디저트식품으로 선호도가 높은 아이스크림 제품류을 제조하고(sherbet 1종, 아이스밀크 2종(IM-2, IM-4), 아이스크림 1종(IC-6)), 각 타락 아이스크림류의 품질 특성을 알아보고자 한다. 타락 아이스크림류의 믹스의 점도는 수분 함량이 가장 높은 Sherbet이 가장 낮았고, 고형분 함량이 가장 높은 IC-6이 유의적으로 높았다. 오버런은 10분에서는 유지방함량이 높았던 IC-6이 높은 값을 나타냈고, 최종 30분에서는 모든 시료간의 유의적 차가 없었다. 녹아내리는 정도는 IC-6이 가장 높았고, Sherbet이 가장 낮은 값으로 측정되었다. 유산균수는 Sherbet은 7.32 Log CFU/g으로 유의적으로 낮게 계측되었고, IM-2, IM-4, IM-6는 8.35~8.49 Log CFU/g으로 유의적 차이가 없었다. 타락 아이스크림류 관능적 평가에서 먼저 강도평가에서 우유향에서는 IC-6이 4.10으로 가장 높았고, 신맛은 Sherbet이 4.20, IM-2가 4.10으로 평가되었으며, 단맛은 IC-6이 5.05로 가장 높게 평가되었고, 쓴맛은 IC-6이 1.65로 낮게 평가 되었다. 크리미한 정도는 IC-6이 4.60으로 유의적으로 높게 평가되었고, 바디감도 5.05로 가장 높게 평가되었다. 외관, 맛, 풍미, 조직감의 선호도에서 IC-6이 다른 시료에 비해 유의적으로 높게 평가되었고, 전반적인 선호도에서 IC-6이 5.15로 가장 높게 평가되었으며, Sherbet이 3.75, IM-2가 3.05, IM-4가 2.50으로 평가되어 타락을 첨가한 아이스크림류는 유지방 함량이 높은 아이스크림이 가장 좋은 관능 선호도를 나타내었고, 유지방함량이 중간정도인 아이스밀크류에 비해 유지방 함량이 없는 Sherbet의 관능 선호도가 조금 더 높았다.

• Journal of Microbiology and Biotechnology
• /
• 제17권9호
• /
• pp.1469-1476
• /
• 2007

Jeot-gal is a traditional Korean fermented seafood and has long been used for seasoning. We isolated 188 strains from shrimp, anchovy, and yellow corvina Jeot-gal, and screened sixteen strains that showed strong fibrinolytic activities on a fibrin plate. Among those strains, the strain that had the largest halo zone was chosen and identified as Bacillus licheniformis by using 16S rDNA sequencing and an API CHB kit. The fibrinolytic activity of Bacillus licheniformis was characterized and designated as bpKJ-31. The active component of bpKJ-31 was identified as a 37 kDa protein, designated bacillopeptidase F, by internal peptide mapping and N-terminal sequencing. The optimum activity of bpKJ-31 was shown at pH 9 and $40^{\circ}C$, with a chromogenic substrate for plasmin. It had high degrading activity for the $B{\beta}$-chain and $A{\alpha}$-chain of fibrin(ogen), and also acted on thrombin, but not skim milk and casein. The amidolytic activity of bpKJ-31 was inhibited by 1 mM phenylmethanesulfonyl fluoride, but 1 mM EDTA did not affect the enzyme activity, indicating that bpKJ-31 is an alkaline serine protease, like a plasmin. The bpKJ-31 showed approximately 14.3% higher fibrinolytic activity than the plasmin. These features of bpKJ-31 make it attractive as a health-promoting biomaterial.

• 한국수산과학회지
• /
• 제28권6호
• /
• pp.812-813
• /
• 1995

Lactococcal cells are nutritionally fastidious and thus, generally cultured either in milk or M17 medium (Terzaghi and Sandine, 1975). In this study, Lactococcus cremoris wild-type (KH) and its less­proteolytic mutant (KHA1) cells were grown on the M17 medium or with modified M17 medium by replicated parallel experiments. The modified M17 medium had the same composition as M17 medium, except that lactose was replaced by glucose. Analyses of culture-broth samples, in which the M17 and the modified M17 media were used, were conducted by high-performance liquid chromatography (HPLC). But, working with these media created noisy problems in analyses of samples. Therefore, a new semi-synthetic medium was developed on the basis of nutritional requirements (Morishita et al., 1981). The composition of the semi-synthetic medium determined on the basis of the nutritional requirements and the composition of milk, is presented in Table 1. The composition of M17 medium is also presented and compared in the table. L. cremoris KH and KHA1 cells were grown again on the new synthetic medium containing glucose or lactose. The broth samples were then drawn and analyzed by HPLC. Clearer separations of fermented products were achieved from the new medium than those with the M17 and the modified M17 media. In comparison with the M17 or the modified M17 media, growth on the new medium was good (Kim et al, 1993). Additional fermentations were also carried out at a controlled pH of 7.0, where enhanced growth of lactococcal cells was obtained. In the fermentations, samples were also analyzed for the concentrations of sugar and lactic acid. The results showed that the new synthetic medium was as good as or better than the M 17 and the modified M 17 media. This is because casein hydrolysate in the synthetic medium provided a ready supply of amino acids and peptides for L. cremoris KH and KHA1 cells. Lactic acid bacteria (LAB) including Lactococcal cells have been known to be an effective means of preserving foods, at the same time as giving particular tastes in fields of dairy products. LAB also have always occupied an important place in the technology of sea products, and marine LAB have known to be present in traditional fermented products (Ohhira et al, 1988). To apply the new synthetic medium to marine LAB, two different LAB were isolated from pickled anchovy and pollacks caviar and were grown on the new media in which various concentrations of NaCl $(3, 5, 7 and 10\%)$ added. They were also grown on the medium solution in natural seawater $(35\%o\;salinity)$ and on the solution of natural seawater itself, too. As seen in Fig. 1, Marine LAB were grown best on the synthetic medium solution in natural seawater and the higher concentrations of NaCl were added to the medium, the longer lag-phase of growth profile appeared. Marine LAB in natural seawater were not grown well. From these results, the synthetic medium seems good to cultivate cells which are essential to get salted fish aged. In this study, it showed that the new synthetic medium provided adequate nutrition for L. cremoris KH and KHA1 cells, which have been used as cheese starters (Stadhouders et al, 1988). Using this new medium, the acid production capability of starter cultures could be also measured quantitatively. Thus, this new medium was inferior to the M17 or the modified M17 medium in culturing the cheese starters and in measuring fermentation characteristics of the starter cells. Moreover, this new medium found to be good for selected and well-identified marine LAB which are used in rapid fermentations of low-salted fish.

• 대한가정학회지
• /
• 제5권1호
• /
• pp.733-739
• /
• 1966

A 371 agricultural households from 26 different communities in South Korea was subjected on a study of food taboos in January of 1966. To the pregnant women, those to whom a high protein diet is particurally important, as many as 14 different kinds of foods, mostly portein rich foods, were avoided to eat. It is believed that if duck is eaten while pregnant her baby may walk like a duck in later life. Some mother have a strong aversion to the rabbit meat that her unborn baby must be a harelip. It is feared to eat chicken, shark or carp by the pregnant mother for her baby may get a gooseflesh appearance, or fish scale-like skin in later life. It is thought that if mother eats soup made of meat borns, especially chicken bones, a disfigured baby may be born. Some area informed that if mother eats crab meat her future baby will always bubble. To the child-bearing mothers 13 different kinds of foods were avoided to eat. Some believe that if raddish kimchi, soybean curd, squash are eaten while dilivery that mother may get dental decay or to lose all her teeth. Other think that highly spiced raddish kimchi cause delivery difficult. To the lactating mothers 7 different items of foods were not recommended to eat. It is a common belief that eating green vegetables, especially fresh lettuce, are restricted that her baby may stool greenish. It is said that eating ginsen-chicken soup, or ginsen tea during lactating reduces breast milk secretion. To the weaning babies 7 different kinds of foods were prohibited to fee. Eggs are not eaten because mothers think her babies will start to talk very late. Eight different items of foods in cases of gastro-intestinal diseases, 5 items for liver disease, 7 items for high blood pressure as well as for paralysis were respectively restricted. It is said that meats including pork, beef, and chicken are neither desirable for the patients of high blood pressure nor those of paralysis. To the measles children 10 varieties of foods were restricted. Especially soybean products and meats were not encouraged to use for avoiding asecond attack of measles. For the common cold 8 different kinds of foods were aversed and men think that eating of soup of undria delays a recovery. For the tuberculosis 4 kinds of foods were prohibited to eat. It is said that wine, red pepper and ginsen will stimulate lung bleeding. Many mothers had a strong aversion to fermented shrimp and fish in case of style. and 5 different items of foods were restricted. In case of menstration not so many foods were restricted as other cases, but meat soup is not eaten in this condition in some areas. Majority of food taboos in Korean villages are neither based on tribal nor religious factors. But no one knows how, since what ages, from where, these food taboos have been transmitted and spread over the country. This survey found a great variety of food taboos, aversions, traditional beliefs and prohibitions latent unknown reseasons, or non-scientific conceptions, or completely different ideas from the modern medical aspect, or somewhat fallacious and superstitious beliefs. For the vascular disease contrasting approach were found between modern the oritical therapy and popular remedy among the rural populations who largely depend on the eastern medication. Further scientific study on either side should be done to lead the patient proper way. Many restricted foods such as rabbit, duck, chicken and fish are best resources of protein rich foods which are available in the village. Emphasis should be laid upon breaking down fallacious and supersititious food taboos through the extended nutrition education activities in order to improve food habit and good eating pattern for healthier and stronger generations of Korea.

• 한국식품위생안전성학회지
• /
• 제24권4호
• /
• pp.318-323
• /
• 2009

다히(dahi)는 원유를 이용하여 방글라데시 전통 방법으로 발효시킨 요구르트와 비슷한 발효 유제품으로 본 연구는 실험실에서 만든 다히(dahi)와 시장에서 판매되고 있는 다히(dahi)의 품질을 비교하기 위하여 수행되었다. 실험실에서 준비한 다히(dahi)는 (A), Kishoregonj 지역에서 수집한 다히(dahi)는 (B), Gazipur 지역의 다히(dahi)는 (C), Bogra 지역의 다히(dahi)는 (D), Dhaka 지역의 다히(dahi)는 (E, F)로 각각 구분하였다. 소비자 만족 품질은 심사위원들에 의해 100점 만점으로 판단되었으며 화학적 미생물학적 품질 시험은 확립된 방법에 의해 수행되었다. 'A' 타입의 다히(dahi)가 소비자 만족 품질에 있어서 가장 우수한 것으로 나타났으며 그 다음으로 E, D, F, B, C 순으로 나타났다. 화학적 시험 결과, 'B'타입의 다히(dahi)가 가장 낮은 pH ($3.75\;{\pm}\;0.05$)를 나타냈으며 'C'타입의 다히(dahi)가 가장 높은 pH ($4.46\;{\pm}\;0.15$)를 나타내었다. 'F' 타입의 다히(dahi)의 총 고형물, 지방, 단백질, 회분이 각 각 $318.40\;{\pm}\;4.44\;g/kg$, $52.00\;{\pm}\;2.00\;g/kg$, $44.33\;{\pm}\;2.00\;g/kg$, $10.76\;{\pm}\;0.31\;g/kg$으로 가장 높게 나타났다. 'B'타입의 다히(dahi)에서 total viable count (TVC)는 $94.00\;{\pm}\;4.58\;cfu/ml$, 효모가 $183.33\;{\pm}\;15.28\;cfu/ml$, 곰팡이가 $53.33\;{\pm}\;15.28\;cfu/ml$로 미생물학적 품질 측면에서 가장 좋지 않은 것으로 나타났다. 'A' 타입의 다히(dahi)는 TVC, 효모와 곰팡이가 각 각 $39.67\;{\pm}\;4.51\;cfu/ml$, $50.00\;{\pm}\;10.00\;cfu/ml$, $20.00\;{\pm}\;10.00\;cfu/ml$로 나타났다. 3가지 주요 품질을 고려하였을 때 'A'타입의 다히(dahi)가 가장 우수하였으며 그 다음으로 F, D, E, C, B 순으로 나타났다.