• 대한한방종양학회지
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• 제4권1호
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• pp.131-146
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• 1998

To examine the effect of Shiquandabutang on the metastasis of cancer, the following experiments were made. Before the main experiments, the cytotoxicity was measured by putting Shiquandabutang sample in HT1080. Then zymography was made to examine the change of gelatinolytic activity. And western blotting was carried out to examine the changes of Fos, Jun, Ets, the transcription factors of MMP-2, MMP-9, and Erk, JNK on signal transduction pathway to AP-1. Third, in vitro invasion assay with transwells coated by collagen and matrigel was carried out. From the results of the above the following conclusions were obtained. 1. The experimental result about cytotoxicity of Shiquandabutang against HT1080 was as below. The stained cell count after being treated by Shiquandabutang sample $400{\mu}g/ml$ for 24 hours was 0.9% of total cells, and the stained cell count by Shiquandabutang sample $100{\mu}g/ml$ was 1.5% of total cells. Both were near the level of control group which showed 0.6% stained. 2. The result of collagenase assay was as below. In Shiquandabutang sample $400{\mu}g/ml$, MMP-2 was reduced as compared with TPA control group, and the band of MMP-9 induced by TPA disappeared. In Shiquandabutang sample $800{\mu}g/ml$, both bands of MMP-2 and MMP-9 disappeared. 3. The results of western blots for Jun, Fos, Ets, Erk, JNK were as below. In Shiquandabutang sample $200{\mu}g/ml$, Ets was reduced, and Fos were increased. 4. The result of invasion assay was as below. The number of cells which migrated across transwell membrane in Shiquandabutang-treated group was less than that of +TPA control group. From the above results, it was concluded that Shiquandabutang might control the appearing and acting of collagenase not by the MMP-2, -9 promoter but by other way.

• 소성∙가공
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• 제25권1호
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• pp.36-42
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• 2016

Fiber metal laminates (FMLs) are well known for improved fatigue strength, better impact resistance, superior damage tolerance and slow crack growth rate compared to traditional metallic materials. However, defects and loss of strength of a composite material can occur due to the vertical load from the punch during the joining with a dissimilar material using a conventional clinching method. In the current study, tapered-hole clinching was an alternative process used to join Al 5052 and FMLs. The tapered hole was formed in the FML before the joining. For the better understanding of static and dynamic characteristics, a clinched joining followed by a tensile-shear test was numerically simulated using the finite element analysis. The design parameters were also evaluated for the geometry of the tapered hole by the Taguchi method in order to improve and compare the lateral joining strength of the clinched joint. The influence of the neck thickness and the undercut were evaluated and the contribution of each design parameter was determined. Then, actual experiments for the joining and tensile-shear test were conducted to verify the results of the numerical simulations. In conclusion, the appropriate combination of the design parameters can improve the joining strength and the cross-sections of the tapered-hole clinched joint formed in the actual experiments were in good agreement with the results of the numerical simulations.

• International Journal of Fluid Machinery and Systems
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• 제5권3호
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• pp.134-142
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• 2012

The competition to deliver ultra-low emitting vehicles at a reasonable cost is driving the automotive industry to invest significant manpower and test laboratory resources in the design optimization of increasingly complex exhaust after-treatment systems. Optimization can no longer be based on traditional approaches, which are intensive in hardware use and laboratory testing. The CFD is in high demand for the analysis and design in order to reduce developing cost and time consuming in experiments. This paper describes the development of a comprehensive practical model based on experiments for simulating the performance of automotive three-way catalytic converters, which are employed to reduce engine exhaust emissions. An experiment is conducted to measure species concentrations before and after catalytic converter for different loads on engine. The model simulates the emission system behavior by using an exhaust system heat conservation and catalyst chemical kinetic sub-model. CFD simulation is used to study the performance of automotive catalytic converter. The substrate is modeled as a porous media in FLUENT and the standard k-e model is used for turbulence. The flow pattern is changed from axial to radial by changing the substrate model inside the catalytic converter and the flow distribution and the conversion efficiency of CO, HC and NOx are achieved first, and the predictions are in good agreement with the experimental measurements. It is found that the conversion from axial to radial flow makes the catalytic converter more efficient. These studies help to understand better the performance of the catalytic converter in order to optimize the converter design.

• 한국컴퓨터정보학회논문지
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• 제24권12호
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• pp.1-7
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• 2019

머신 러닝의 심층 개발로 딥 러닝 방법은 특히 CNN(Convolution Neural Network)에서 큰 진전을 이루었다. 전통적인 텍스트 정서 분류 방법과 비교할 때 딥 러닝 기반 CNN은 복잡한 다중 레이블 및 다중 분류 실험의 텍스트 분류 및 처리에서 크게 발전하였다. 그러나 텍스트 정서 분류를 위한 신경망에도 문제가 있다. 이 논문에서는 LSTM (Long-Short Term Memory network) 및 CNN 딥 러닝 방법에 기반 한 융합 모델을 제안하고, 다중 카테고리 뉴스 데이터 세트에 적용하여 좋은 결과를 얻었다. 실험에 따르면 딥 러닝을 기반으로 한 융합 모델이 텍스트 정서 분류의 예측성과 정확성을 크게 개선하였다. 본 논문에서 제안한 방법은 모델을 최적화하고 그 모델의 성능을 개선하는 중요한 방법이 될 것이다.

• Archives of Pharmacal Research
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• 제26권11호
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• pp.917-924
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• 2003

This research aims to test a new drug candidate based on a traditional medicinal herb, F1, an herbal extract obtained from Astragalus membranaceus and its main ingredient, 1-monolinolein that may have fewer side effects and less uterine hypertrophy. In vitro experiments, human osteoblast-like cell lines, MG-63 and Saos-2, were analyzed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and an alkaline phosphatase (ALP) assays. Mouse osteoclasts were induced through a calcium-deficient diet and inhibition effects were measured. In vivo experiments were done using ovariectomized (OVX) rats for 9 weeks. At necropsy, uterus weights were measured, trabecular bone area (TBA) of tibia and lumbar vertebra were measured bone histomorphology. In results, cell proliferation and ALP activity in Saos-2 by ether F1 or 1-monolinolein did not increased significantly compared to the control. The F1 inhibited osteoclast development ($IC_{25}=3.37{\times}10^{-5}$mg/mL) less than 17$\beta$-estradiol. The OVX rats administered F1 (2 mg/kg/day and 10 mg/kg/day) showed an increase in TBA of the tibia significantly (136.3${\pm}4.2% and 138.5{\pm}$10.3% of control). In conclusions, the herbal extract, F1 inhibited tibia and lumbar bone loss and did not cause uterine hypertrophy. However, 1-monolinolein, the main ingredient of the herbal extract, did not inhibit bone loss.

• BMB Reports
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• 제41권5호
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• pp.369-375
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• 2008

Polygonatum cyrtonema Lectin (PCL), which is classified as a monocot mannose-binding lectin, has received great regards for its uniquely biological activities and potentially medical applications in cancer cells. This paper was initially aimed to study apoptosis of PCL on Hela cells. Thus, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method was carried out. Through observation of cell morphologic changes and Lactate dehydrogenase (LDH) activity-based cytotoxicity assays, PCL induced HeLa cell apoptosis in a dose-dependent manner. To further gain structural basis, multiple alignments, homology modeling and docking experiments were performed to analyze the correlation between its biological activities and mannose-binding sites. Eventually, considering docking data, chemical modification properties on the three mannose-binding sites were analyzed by a series of biological experiments (e.g., hemagglutinating and mitogenic activity assays, fluorescence and Circular Dichrosim (CD) spectroscopy) to profoundly identify the role of some key amino acids in the structure-function relationship of PCL.

• 한국공간정보시스템학회 논문지
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• 제11권4호
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• pp.56-64
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• 2009

최근 웹 2.0 기술의 활용이 증가하면서 RIA (Rich Internet Application) 기술에 대한 중요성도 증가하고 있다. RIA는 인터넷 서비스가 가능한 데스크탑 및 모바일 기기에서 다양한 정보를 제공할 수 있는 장점이 있다. 본 논문에서는 RIA 기술 중에서 플래시를 사용하여 공간 데이터를 모바일 환경에서 서비스하는 방법을 제안한다. 공간 데이터 서버는 사용자가 요구한 공간 데이터를 RIA 기술 중 하나인 플래시 기반의 웹 어플리케이션에 포함하여 제공하는 방법을 사용한다. 본 논문에서 제안하는 방법은 클라이언트에서 렌더링 및 사용자 인터페이스 처리를 담당하는 특징을 가진다. 또한, 서버로부터 웹 어플리케이션을 다운로드 받으면 추가로 공간 데이터 서버에 접속 없이도 클라이언트 자체에서 공간 데이터의 패닝, 확대/축소 등의 처리가 가능하므로 무선 언터넷 환경을 사용하는 모바일 장치에서 효과적이다. 그리고, 풀랫폼에 독립적으로 동작되어 모바일 환경에서도 데스크탑 환경과 동일하게 활용할 수 있는 장점이 있다. 본 논문에서 제안하는 공간 데이터 서버의 성능을 평가하기 위하여 WMS(Web Map Server)와 비교 분석한다.

• 한방재활의학과학회지
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• 제18권2호
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• pp.157-167
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• 2008

Objectives : It is sure that standardization of medical instruments is very important and necessary. Cupping therapy is one of the traditional oriental medical method, and it is necessary to set up a standard of cupping therapy and instruments. Methods : We select 3 major cupping instrument makers according to a questionnaire survey of 2001 oriental medical doctors. Dae Gun is A maker, Han Sol is B maker, Dong Bang is C maker. And with these air pumps we make experiments on stability by an inner impact test and a falling test. With these cups we check stability by an inner impact test and a disinfection test. And to know function of these instruments we test a negative pressure and maintain attached cups until 5 to 10minutes. Results : All results of experiments(impacted test, disinfection test, falling test) on stability of cups and air pumps are suitable for medical instrument. And the efficiency of 3 major makers shows various results. Negative pressure of B group is the highest, C group is the second, A group is the lowest. Pressure maintenance test shows A and C group is comparatively up to the experimental standard(<10%), B group is comparatively below the experimental standard(<10%). Conclusions : Results show stability of cups and air pumps is suitable for medical instruments. And negative pressure of cupping and maintenance of pressure show various results. So it is necessary to set up a standard negative pressure of cupping and maintenance of pressure.

• Journal of Electrical Engineering and Technology
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• 제13권1호
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• pp.468-475
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• 2018

In medical ultrasound imaging, frequency-dependent attenuation downshifts and reduces a center frequency and a frequency bandwidth of received echo signals, respectively. This causes considerable errors in quadrature demodulation (QDM), result in lowering signal-to-noise ratio (SNR) and contrast resolution (CR). To address this problem, adaptive dynamic QDM (ADQDM) that estimates center frequencies along depth was introduced. However, the ADQDM often fails when imaging regions contain hypoechoic regions. In this paper, we introduce a valid region-based ADQDM (VR-ADQDM) method to reject the misestimated center frequencies to further improve SNR and CR. The valid regions are regions where the center frequency decreases monotonically along depth. In addition, as a low-pass filter of QDM, Gaussian wavelet based dynamic filtering was adopted. From the phantom experiments, average SNR improvements of the ADQDM and the VR-ADQDM over the traditional QDM were 1.22 and 5.27 dB, respectively, and the corresponding maximum SNR improvements were 2.56 and 10.58 dB. The contrast resolution of the VR-ADQDM was also improved by 0.68 compared to that of the ADQDM. Similar results were obtained from in vivo experiments. These results indicate that the proposed method would offer promises for imaging technically-difficult patients due to its capability in improving SNR and CR.

• 대한원격탐사학회지
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• 제26권1호
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• pp.29-38
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• 2010

높은 공간 해상도의 지상 자료와 상대적으로 저해상도인 원격탐사 자료의 통합을 통한 지표 환경 주제도 작성에서는 이러한 해상도의 차이를 반영한 통합 방법론이 필요하다. 이 연구에서는 상대적으로 저해상도인 원격탐사 자료와 지상 자료로부터 고해상도 주제도 작성과 관련된 다운스케일링을 위한 다중 스케일 지구통계학적 방법론을 적용하였다. 기존 정규 크리깅 시스템을 확장하여 포인트 자료로 간주할 수 있는 지상 자료와 블럭 자료로 간주할 수 있는 원격탐사 자료를 크리깅 시스템에 직접 포함하는 블럭 크리깅 방법론을 이용하였다. 부가적으로 다운스케일링에 따른 불확실성을 묘사하기 위해 블럭 크리깅 기반 시뮬레이션 기법도 함께 이용하였다. SRTM DEM과 MODIS 엽면적 지수 자료의 다운 스케일링 실험 연구를 통해 적용 기법의 적용성을 평가하였다. 두가지 실험 연구 결과, 적용 기법을 통해 효과적으로 상대적으로 고해상도 주제도 생성이 가능함을 확인하였으며, 특히 다중 시뮬레이션 결과는 다운 스케일링된 자료를 입력 자료로 사용하는 GIS 모델에 사용하여 모델 결과의 불확실성 분석에 효율적으로 이용될 수 있을 것으로 기대된다.