• Title/Summary/Keyword: trace analysis

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A Study on Wound Healing Factors in Diabetic Foot Ulcer Patients (당뇨발 궤양을 가진 환자에서의 창상치유인자에 대한 조사)

  • Gu, Ja Hea;Han, Seung-Kyu;Chang, Hyun;Lee, Byoung Il;Kim, Woo Kyung
    • Archives of Plastic Surgery
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    • v.34 no.5
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    • pp.543-550
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    • 2007
  • Purpose: Diabetic foot ulcer is a representative disease of chronic would with multiple defects of wound healing factors. Many nutrition factors have been known to be essential for wound healing, but objective data are lacking. The purpose of this study is to determine those factors essential for wound healing, and to find out which of those factors are lacking in diabetic foot ulcer patients through this pilot study. Methods: We studied 100 patients who visited our clinic from March 2005 to February 2006 for treatment of diabetic foot ulcers with a duration of more than 6 weeks. We checked serum levels of protein, albumin, vitamin A, C, E, iron, magnesium, zinc, copper and hemoglobin by drawing 23cc blood after 8 hours of fasting. Protein, albumin, iron, magnesium levels were measured by colorimetry; hemoglobin levels were measured by auto analysis. Vitamin levels were measured by high performance liquid chromatography (HPLC), copper and zinc levels were measured by Inductively coupled plasma (ICP). They were compared with normal values. The patients were divided by transcutaneous oxygen pressure levels, age and sex to study the effects of these parameters. Results: 76% and 61% of patients had within-normal range serum protein and albumin levels, respectively. Among vitamins, only the level of vitamin C was low in 55% of the patients. Levels of vitamin A, E were normal or high in 93% and 100% of patients. As for trace elements, levels of iron and zinc were low in 63% and 60% of patients, but levels of magnesium and copper were usually normal or high. Levels of vitamin C, iron and zinc were lower in the low-transcutaneous oxygen pressure group. There were no definite differences according to age and sex. Conclusion: Serum levels of Hb, vitamin C, iron, zinc were low in most diabetic foot ulcer patients. The deficit was very severe in the low-transcutaneous oxygen pressure group.

Development of SNP Markers for Domestic Pork Traceability (국내산 돼지고기의 원산지 검증을 위한 SNP Marker Set 개발)

  • Kim, Sang-Wook;Li, Xiaoping;Lee, Yun-Mi;Kim, Jong-Joo;Kim, Tae-Hun;Choi, Bong-Hwan;Kim, Kwan-Suk
    • Journal of Animal Science and Technology
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    • v.52 no.2
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    • pp.91-96
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    • 2010
  • The purpose of the study was to develop an optimum SNP marker set to be utilized for domestic pork traceability. The study tested 51 SNP markers analyzed for origin of farm to be determined from genotypes of offspring and parents in pigs. With the simulation data through random mating population (PI), half sib mating population ($PI_{half-sib}$) and full sib mating population ($PI_{sibs}$), probability of identical genotypes were analyzed as $5.63{\times}10^{-33}$, $4.35{\times}10^{-15}$ and $1.32{\times}10^{-15}$, respectively. The 51 SNP markers also had 100% accuracy for parental determination. These results suggest that if the pig breeding stock is genotyped with the 51 SNP markers, the genotype information of individual offspring can be checked for farm origins by tracing parental sow and sire. Therefore, these SNP markers will be useful to trace the pork from production to consumption in pigs.

Interference of Sulphur Dioxide on Balloon-borne Electrochemical Concentration Cell Ozone Sensors over the Mexico City Metropolitan Area

  • Kanda, Isao;Basaldud, Roberto;Horikoshi, Nobuji;Okazaki, Yukiyo;Benitez-Garcia, Sandy-Edith;Ortinez, Abraham;Benitez, Victor Ramos;Cardenas, Beatriz;Wakamatsu, Shinji
    • Asian Journal of Atmospheric Environment
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    • v.8 no.3
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    • pp.162-174
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    • 2014
  • An abnormal decrease in ozonesonde sensor signal occurred during air-pollution study campaigns in November 2011 and March 2012 in Mexico City Metropolitan Area (MCMA). Sharp drops in sensor signal around 5 km above sea level and above were observed in November 2011, and a reduction of signal over a broad range of altitude was observed in the convective boundary layer in March 2012. Circumstantial evidence indicated that $SO_2$ gas interfered with the electrochemical concentration cell (ECC) ozone sensors in the ozonesonde and that this interference was the cause of the reduced sensor signal output. The sharp drops in November 2011 were attributed to the $SO_2$ plume from Popocat$\acute{e}$petl volcano southeast of MCMA. Experiments on the response of the ECC sensor to representative atmospheric trace gases showed that only $SO_2$ could cause the observed abrupt drops in sensor signal. The vertical profile of the plume reproduced by a Lagrangian particle diffusion simulation supported this finding. A near-ground reduction in the sensor signal in March 2012 was attributed to an $SO_2$ plume from the Tula industrial complex north-west of MCMA. Before and at the time of ozonesonde launch, intermittent high $SO_2$ concentrations were recorded at ground-level monitoring stations north of MCMA. The difference between the $O_3$ concentration measured by the ozonesonde and that recorded by a UV-based $O_3$ monitor was consistent with the $SO_2$ concentration recorded by a UV-based monitor on the ground. The vertical profiles of the plumes estimated by Lagrangian particle diffusion simulation agreed fairly well with the observed profile. Statistical analysis of the wind field in MCMA revealed that the effect Popocat$\acute{e}$petl was most likely to have occurred from June to October, whereas the effect of the industries north of MCMA, including the Tula complex, was predicted to occur throughout the year.

The Effect of Thatch Decomposing by Application with Composted Liquid Manure and Microorganism Medium in Golf Course Soil (배양미생물과 가축분뇨발효액비의 시비가 골프코스 토양 중 대취분해에 미치는 영향)

  • Ham, Suon Kyu;Lim, Ji Yeon;Lee, Yeong Min
    • Weed & Turfgrass Science
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    • v.3 no.4
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    • pp.342-346
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    • 2014
  • The purpose of this experiment is for investigating how much the amino acid liquid fertilizer and composted liquid manure, culture microorganism effect on the breeding of grasses and knowing the extent of the thatch content through an analysis of the soil. For testing about soil chemical, the quality of grasses, and the extent of the thatch content in the soil, we cultivated 6 kinds of microorganisms having the effect of thatch dissolution and sprayed these 6 microorganisms with composted liquid manure and the amino acid liquid fertilizer on the place Creeping bentgrass have planted. This conduction started from June to October, 2012 and 2013 (The interval: 2 weeks). In the result of the turf growth, there is no big difference between soil chemical and trace element. And we can know Leaf Color Index, Chlorophyll Index and Root Length are almost same as among treatment. In conclusion, the mixing fertilization of culture microorganism and composted liquid manure is better effective than the traditional fertilization. And it can be expected the effect of the quality of grass and Thatch decomposition in soil.

Simultaneous Determination of Ultra-Trace Pesticides and Synthetic Materials in Surface Water by LC-ESI-MS/MS (하천수에서 LC-ESI-MS/MS에 의한 극미량 농약류 및 합성원료의 동시분석법)

  • Hong, Seon-Haw;Lee, Jun-Bae;Lee, Soo-Hyung;Cho, Young-Hwan;Shin, Ho-Sang
    • Journal of the Korean Chemical Society
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    • v.59 no.3
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    • pp.225-232
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    • 2015
  • A liquid chromatography-electrospray ionization-tandem mass spectrometry method (LC-ESI-MS/MS) was used for determining seven pesticides (2,4-dichlorophenoxyacetic acid, methomyl, aldicarb, 2-methyl- 4-chlorophenoxy- acetic acid, molinate, carbaryl and carbofuran) and two synthetic materials (quinoline and bisphenol-A) in surface water. The analytes were extracted using solid-phase extraction (SPE). The eluate was concentrated by nitrogen gas. 100 microliters of 30% (v/v) methanol aqueous solution were used to dissolve the residue and an aliquot of the reconstituted solution was directly injected into LC-ESI-MS/MS after the filtration using 0.2 μm polytetrafluoroethylene (PTFE) syringe filter. Under the established condition, the calibration curves of the analytes were linear with correlation coefficients of above 0.997. The quantification limit was 0.002~0.011 μg/L and the relative standard deviations were less than 16.4%. In addition, accuracy was in the range of 84~107% and the recoveries were values between 56.2 and 98.6%. In this study, the developed method was applied to the analysis of real surface water samples.

Sol-Gel Synthesis, Crystal Structure, Magnetic and Optical Properties in ZnCo2O3 Oxide

  • Das, Bidhu Bhusan;Barman, Bittesh
    • Journal of the Korean Chemical Society
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    • v.63 no.6
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    • pp.453-458
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    • 2019
  • Synthesis of ZnCo2O3 oxide is performed by sol-gel method via nitrate-citrate route. Powder X-ray diffraction (XRD) study shows monoclinic unit cell having lattice parameters: a = 5.721(1) Å, b = 8.073(2) Å, c = 5.670(1) Å, β = 93.221(8)°, space group P2/m and Z = 4. Average crystallite sizes determined by Scherrer equation are the range ~14-32 nm, whereas SEM micrographs show nano-micro meter size particles formed in ZnCo2O3. Endothermic peak at ~798 K in the Differential scanning calorimetric (DSC) trace without weight loss could be due to structural transformation and the endothermic peak ~1143 K with weight loss is due to reversible loss of O2 in air atmosphere. Energy Dispersive X-ray (EDX) analysis profile shows the presence of elements Zn, Co and O which indicates the purity of the sample. Magnetic measurements in the range of +12 kOe to -12 kOe at 10 K, 77 K, 120 K and at 300 K by PPMS-II Physical Property Measurement System (PPMS) shows hysteresis loops having very low values of the coercivity and retentivity which indicates the weakly ferromagnetic nature of the oxide. Observed X-band EPR isotropic lineshapes at 300 K and 77 K show positive g-shift at giso ~2.230 and giso ~2.217, respectively which is in agreement with the presence of paramagnetic site Co2+(3d7) in the oxide. DC conductivity value of 2.875 ×10-8 S/cm indicates very weakly semiconducting nature of ZnCo2O3 at 300 K. DRS absorption bands ~357 nm, ~572 nm, ~619 nm and ~654 nm are due to the d-d transitions 4T1g(4F)→2Eg(2G), 4T1g(4F)→4T1g(4P), 4T1g(4F)→4A2g(4F), 4T1g(4F)→4T2g(4F), respectively in octahedral ligand field around Co2+ ions. Direct band gap energy, Eg~ 1.5 eV in the oxide is obtained by extrapolating the linear part of the Tauc plot to the energy axis indicates fairly strong semiconducting nature of ZnCo2O3.

Development of Teas from Leaves of Korean Box thorn (Lycium chinense Miller) Leaves (구기엽을 이용한 차류의 개발)

  • Kim, Seung Yeol;Lee, Kyong Haeng;Kim, Seung Kyeom
    • Korean Journal of Agricultural Science
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    • v.24 no.1
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    • pp.29-40
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    • 1997
  • Six different types of teas from box thorn leaves collected on mid May were prepared by using different tea making procedures. The selected physicochemical propentes and the sensory qualities of the 6 different teas were analyzed. The box thorn leaves contained 87.71% moisture, 0.69% crude fat, 4.77% crude protein, 2.13% fibers, 3.11% carbohydrates and 1.59% ash. Based on the sensory analysis, roasted tea(duckum tea) had the highest values in taste, flavor, color and overall preference. Eighteen free amino acids were identified in the roasted tea, showing the specially high levels of proline(170.15mg%) and glutamic acid(112.34mg%). The roasted tea also contained glucose(1.07%), maltose(0.87%), sucrose(0.63%) and fructose(0.55%). The content of citric acid(337.43mg%) in the roasted tea was the highest, followed by malonic acid(54.17mg%), oxalic acid(48.66mg%), malic acid(27.41mg%), succinic acid(4.48mg%), fumaric acid(1.08mg%) and lactic acid(trace amount), in a decreasing order.

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Drinking conditions of adolescents ; Korea Youth Risk Behavior Web-based Survey (청소년의 음주 실태 ; 청소년 건강행태 온라인 조사를 중심으로)

  • Choi, Young-Sil
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.9
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    • pp.186-192
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    • 2017
  • Thepurpose of this study was to provide a basic data health plan & education program for adolescents by examining the recent 3 year Korea Youth Risk Behavior Web-based Survey data obtained in 2013, 2014 and 2015. The data was analyzed with theSPSS 18.0 statistical program using the t-test and cross-analysis. After confirming the drinking habits of teenagers in Korea, the issue was slightly lower in the issue of problem drinking. However, the first time for drinking alcohol was the 2nd grade of middle school(21.5%) and the average alcohol intake was 20 to 29 days(3.8%) and usually consisted ofmore than two bottles (12.2%)s. Also, the method of buying liquor was found to be mainly at convenience stores(32%), and the adolescents werefound not to have been trained for drinking(52%). As a result of comparing the general characteristics of these items, it was found that there was a significant difference between the coeducation type, the high school type, the trace in the residence type, and the 'middle-high level' in terms of the socio-economic level.When comparing the results of the drinking behavior during these three years, the outcome of the trend is still noteworthy, as health education for drinking alcohol is still important, and attention needs to be paid to this problem.

A Study on Relation of Attached Algae and Odor's Cause in Sedimentation Basin of Water Treatment Plant (정수처리공정 중 침전지 부착조류 특성과 이취발생에 관한 연구)

  • Kim, Young-Ung;Son, Hee-Jong;Yu, Myung-Ho;Lee, Chun-Sik;Cho, In-Cheol;Kim, Eun-Ho;Seung, Nak-Chang
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.5
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    • pp.887-894
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    • 2000
  • The purposes of this study are investigation of species of attached algae and the relation between attached algae and odor in tap water, Oscillatoria sp. and Mougeotia sp. were colonized at sedimentation basin in water treatment plant. Resulting from analysis, 3.7ppt of geosmin and dimethyl-disulfide with trace were detected in sample of Oscillatoria sp. only, 22 compounds were found in each of sample that had been incubated in room temperature for 5 days, 17 compounds were generated by algal decay. Decayed products were sulfur compounds of 10 species such as dimethyl-sulfide and phenol, p-cresol, indole and scatole. So, it is assumed that attached algae which tolerant to chlorine was the origin of odor in tap water. They usually formed big colonies. Colonies on the bottom were decayed because of anaerobic state. While decaying they were detached from colonies and so, odorous compounds are originated from this decayed algae.

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Material Characteristics of White Wares from Yeongdong Province, Gangwon-do: Gangneung and Donghae Kiln Sites (강원 영동지역 백자의 재료과학적 특성 연구: 강릉 남양리 백자가마터와 동해 발한동2(사문동) 가마터를 중심으로)

  • Lee, Byeong Hoon;So, Myoung-Gi
    • Journal of Conservation Science
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    • v.31 no.3
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    • pp.181-192
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    • 2015
  • This study aims to examine production technique of twelve white wares from the Gangneung Namyang-ri and Donghae Balhan-dong2(Samun-dong) kiln sites, Kangwon Province and characteristics of the used materials, and to find a correlation among materials of the excavated white wares. X-ray fluorescence sequential spectroscopy(XRF), X-ray diffraction(XRD), Dilatometer and Inductively coupled plasma mass spectrometry(ICP-MS), Inductively coupled plasma automic emission spectrometer(ICP-AES) were applied to determine the chemical composition, crystalline phase of samples, firing temperatures, trace elements and rare earth elements. When analyzing body crystalline phases of the white wares using the XRD method, quartz and mullite were extracted from all the samples. Though firing temperature of each sample was different, they were mostly fired at a temperature below $1200^{\circ}C$. Analyzing the excavated white wares using the Seger formula, compositional properties of white wares in Gangneung Namyang-ri kiln showed diffrently from the Donghae Balhan-dong2(Samun-dong) kiln. The body of white wares from Gangneung Namyang-ri kiln have higher raito of $RO_2$ and $RO+R_2O$ than of white wares from Donghae Balhan-dong2(Samun-dong) kiln site. The white wares from Gangneung Namyang-ri kiln and Donghae Balhan-dong2(Samun-dong) kiln were made of host rocks of the different geological origin, according to the result of rare earth elements analysis.