• 제목/요약/키워드: trabeculae

검색결과 121건 처리시간 0.025초

성견치주질환 이환 발치와내 이식 치근과 발치와 치조골 재생에 대한 연구;I. 치근활택술의 영향 (REGENERATION OF THE ALVEOLAR BONE AND TRANSPLANTED ROOTS INTO THE PERIODONTALLY INVOLVED EXTRACTION SOCKETS IN DOGS;I : EFFECT OF ROOT PLANING PROCEDURE)

  • 김종관;채중규;조규성;김진;한수부;최상묵
    • Journal of Periodontal and Implant Science
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    • 제24권1호
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    • pp.64-86
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    • 1994
  • The authors have transplanted periodontally involved roots which had been root planed into healthy and periodontally involved extraction sockets, and studied the root resorption patterns as well as its effect on new bone formation and wound healing. Alveolar bone around mandibular premolars of 6 adult dogs has been surgically removed, followed by ligation of orthodontic elastic wires for 8 weeks inducing chronic periodontal disease. After removing the crown portions, roots were extracted, and notches were made on the root surfaces discriminating healthy and periodontally involved areas using burs. Controls and experimental groups were divided as follows. Control I : Transplantation of periodontally involved root into healthy extraction sockets. Control II : Transplantation of periodontally involved root into diseased extraction sockets. Experimental group I : Transplantation of root planed roots into healthy extraction sockets. Experimental group II : Transplantation of root planed roots into diseased extraction sockets. Extraction sockets were sutured after transplantations, completely submerging the roots. Healing progress was histologically observed at 2nd, 8th, 12th, and 20th weeks, and the results were as follows ; 1. No inflammation or infection within the extraction sockets had been observed in all groups throughout the experimental period. 2. Reversal lines were observed at week 2 in all groups, clearly discriminating socket walls and new bone, and numerous blood vessels were observed in the new bone trabeculae. 3. Experimental groups showed markedly less root resorption compared to the controls at week 2, but as time progressed, severe resorptions were present in all groups. 4. Localized areas of new bone ankylosis were observed, and the rest of the areas showed collagen fiber insertion with new bone formation at its periphery. 5. No clear differences were found in healing and alveolar bone regeneration between healthy and diseased extraction sockets. 6. The amount of root resorption and ankylosis had increased up to week 8 and 12, showing ankylosis of new bone and the roots. However, no further increase in ankylosis was observed at week 20. 7. Most of the cementum on healthy roots was directly ankylosed to new bone at week, 2, and were gradually resorbed and replaced by new bone thereafter. These results appear to indicate that root planing may inhibit early root resorption of transplanted roots, but gradual replacement by alveolar bone and collagen fibers eventually occur. Condition of the roots or presence of disease in extraction sockets do not appear to make marked differences in alveolar bone regeneration process.

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우(牛), 마(馬), 돈(豚) 및 견(犬)의 실질장기내(實質臟器內) Mast Cell 분포(分布)와 Rompun을 투여(投與)한 견(犬)의 Mast Cell 탈과립소견(脫顆粒所見) (Distribution of the Mast Cells in the Parenchymal Organs of the Cattle, Horses, Pigs, Dogs, and Rompun-induced Mast Cell Degranulation in the Dog)

  • 김태환;이차수
    • 대한수의학회지
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    • 제25권2호
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    • pp.113-123
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    • 1985
  • This paper dealt with the distribution of normal mast cells in the spleen, liver and lung on cattle, horses, pigs and dogs, and also degranulation of mast cells in the dogs infected with Rompun (2% Xylazine HCl). The results observed are summarized as follows. Normal mast cells were distributed in spleen, liver and lung on cattle, horse, pig and dog. Mast cells were observed in both red pulp and surroundings of white pulp of the spleen in horse, in the white pulp of the spleen in cattle, in the trabeculae of the spleen in pigs, and in white pulp and red pulp of the spleen in dogs, respectively. Mast cells were observed in the portal triad of the liver in cattle and horses, in both portal triad and interlobular connective tissues of the liver in pigs, and not only the portal triad but also walls of the sinusoids and the central veins in dogs. A large number of mast cells were observed in the interlobular septa and peribronchioles of lung on all the species in this experiment. The mast cells are more numerous in the lungs than other organs. Author considers that numbers of normal mast cells distributed in the tissue is related to the dosage of Rompun in animal. The degranulation of mast cells were observed in the subcutaneous tissues of dog intramuscularly injected with Rompun(0.5ml/times) for 4 or 5 times and subcutaneously injected with Rompun(0.3ml/times) for 4 times. In dog intradermally injected with 0.1ml of Rompun, mast cells were decreased in number at 30 minutes and markedly decreased in number at 2 hours, but more or less increased in number at 3 hours after injection. In addition, the granules of the mast cells were decreased in number at 30 minutes and marked degranulation of the mast cells were recognized at 2 hours after injections, but normal mast cells begun to appear in subcutaneous tissue with the lapse of time from 3 hours after injection. There was also observed local infiltration of neutrophils in subcutaneous tissues of dogs intradermally injected with 0.1ml of Rompun at 30 minutes. At 2 hours after injection, numerous neutrophils and a small number of eosinophils were observed in the site of injection. Conclusionally, Rompun was regarded as a factor which causes the degranulateon of mast cell and the authors considered that histamine released from the mast cells by Rompun might cause relaxation of skeletal muscle.

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전기자극이 정상 치주조직에 미치는 영향 (EFFECTS OF ELECTRICAL STIMULATION ON THE NORMAL PERIODONTIUM)

  • 임경석;권영혁;이만섭;박준봉
    • Journal of Periodontal and Implant Science
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    • 제32권1호
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    • pp.89-112
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    • 2002
  • The earliest reports of the use of electrical energy to directly stimulate bone healing seem to be in 1853 from England, the techniques involved the introduction of direct current into the non-united fracture site percutaneously via metallic needles, with subsequent healing of the defect. One endpoint of the periodontal therapy is to generate structure lost by periodontal diseases. Several procedural advances may support regeneration of attachment, however, regeneration of alveolar bone does not occur consistently. Therefore, factors which stimulate bone repair are areas for research in periodontal reconstructive therapy. Effects of cytokines or growth factors on bone repair are examples of such areas. Another one is electrical current which occurs in bone naturally, so that such bone may be particularly susceptible to electrical therapy. The purposes of this study were to observe the effects of electrical stimulation on the normal periodontium, to determine whether the electricity is the useful means for periodontal regeneration or not. Forty rats weighted about 100 gram were used and divided into 4 groups, the first group, there was no electrical stimulation with the connection of electrodes only. In the second group, there was stimulated by the 10 mA during 10 minutes per a day, in the third group was stimulated by the 25 mA , and the fourth by the 50 mA. At 3, 5, 10 and 15 days post-appliance , two rats in each group were serially sacrificed. and the maxillae and the mandible processed to paraffin, and the specimens were prepared with Hematoxylin-Eosin stain for the light microscopic evaluation. The results of this study were as follows : 1. There was the distinct reversal line on the lingual alveolar crest, whereas a little changes in the labial alveolarcrest to the duration and amount of currents. 2. In 50 mA group, the cells were highly concentrated at the apex of anterior teeth, and was observed the necrotic tissue. In posterior root apex, the hypercementosis was appeared, and newly formed cementum layer has been increased continuously with the time. 3. The periodontal ligament fiber and Sharpey's fiber were arranged in order, and the bone trabeculae were increased as the experiment proceeded by, relatively the bone marrows were decreased. 4. In the pulp tissue, the blood vessels were increased with blood congestion in the experimetal specimens remarkably, and the dentinal tubules were obstructed . 5. The osteoblasts in alveolar bone proper had been showed highly activity, and also observed the formation of bone trabeculea. In the conclusion, it was suggested that the electrical stimulation has influence on the periodontium and the pulp tissue. However, there might be the injurious effects.

토끼 경골에서 치과용 임프란트의 이중 산부식 및 양극 산화 표면처리에 따른 조직계측학적 연구 (HISTOMORPHOMETRIC STUDY OF DENTAL IMPLANTS WITH DOUBLE ACID-ETCHED AND ANODIC OXIDIZED SURFACE IN THE RABBIT TIBIA)

  • 한예숙;김일규;장금수;박태환;전원
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제28권5호
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    • pp.434-444
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    • 2006
  • This study was performed to evaluate the effects of three different implant surface treatments to the bone formation during osseous healing period under unloading conditions. Machined, double-acid etched and anodic oxidized implants were inserted into tibia of 3.0 - 3.5 kg NZ white male rabbits and 2 animals of each group were sacrificed at 2, 4 and 8 weeks. The specimens containing implant was dehydrated and embedded into hard methylmethacrylate plastic. After grinding to $50{\mu}m$, the specimens were stained with Villanueva bone stain. From each specimen, histomorphometric evaluation and the bone implant contact rate were analysed with optical microscope. The results were as follows; 1. In the scanning electronic microscopic examination, machined surface implant had several shallow and paralleled scratches on plain surface, double acid-etched implant had lots of minute wrinkles, rough valley and also irregularly located craters that looked like waves, anodic oxidized surface implant had porosity that minute holes were wholly distributed on the surface. 2. After 2 weeks of implantation, the percentages of bone-to-implant contact in the machined implant, double acid-etched implant and anodic oxidized implant were 26.85%, 62.64% and 59.82%, after 4 weeks of implantation they were 64.29%, 77.85% and 75.23%, and after 8 weeks they were 82.66%, 85.34% and 86.39%. 3. After 2 weeks of implantation, the percentages of bone area between threads in the machined implant, double acid-etched implant and anodic oxidized implant were 21.55%, 42.81%, and 40.33%, after 4 weeks of implantation they were 49.32%, 62.60% and 75.56%, and after 8 weeks they were 71.62%, 87.73% and 83.94%. In summary, percentages of implant surface contacted to bone trabeculae and bone formation area inside threads in double acid-etched implants and anodic oxidized implants were greater than machined implants in early healing stage. These results suggest that double acid-etched and anodic oxidized surface implants could reduce the healing period for osseointegration and may enable to do early function.

하악골에 발생한 거대한 cemento-ossifying fibroma의 치험례 (A CASE REPORT OF THE HUGE CEMENTO-OSSIFYING FIBROMA OF THE MANDIBLE)

  • 이상철;김여갑;류동목;이백수;권용대;박종오
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제22권1호
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    • pp.70-75
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    • 2000
  • 본과에서는 하악에서 발생한 거대한 백악질-골성 섬유종에서 원발병소부위에 대한 광범위한 외과적 적출술 및 후방 장골이식을 통해 환자의 심미적, 기능적 안정성을 이루었으며 이환치아의 발거와 함께 술후 악간고정을 통하여 얇아진 하악하연부의 병적골절을 방지하였다. 현재까지 병소의 재발은 없고 양호한 결과를 보이고 있으며 향후 보철수복과 함께 지속적인 평가를 요할 것으로 사료된다.

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가토에서 하악골신장술시 신장부에 대한 가골압축의 효과 (EFFECTS OF COMPRESSION ON DISTRACTED AREA DURING MANDIBULAR DISTRACTION IN RABBITS)

  • 박홍주;안진석;국민석;오희균;유선열;조진형
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제29권1호
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    • pp.1-9
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    • 2007
  • Purpose: To evaluate the effect of compression on the distracted area in new bone formation during mandibular distraction osteogenesis in rabbits. Materials and method: Sixteen rabbits, weighing approximately 2 Kg, and the prefabricated distraction device were used. With the rabbits under general anesthesia, we performed vertical osteotomies between the anterior and posterior mandibular teeth and then placed the distraction device. After a 5 -day latency period, the mandible was distracted to a length of 10.0 mm at a rate of 1 mm/day and then immediately compressed 4 mm in the experimental group (n=8). In the control group (n=8), the mandible was distracted to a length of 6.0 mm at a rate of 1 mm/day. Rabbits in the control group were killed at 2 and 8 weeks during the consolidation period. The specimens were evaluated with light microscope after H & E stain. Histomorphometric analysis was done at 8 week specimens. Results: All experimental animals showed mandibular elongation on the macroscopical and radiographic evaluations. At 2 week, immature bone formation was observed from the surface of the host bone margins with collagen fibers arranged parallel to the direction of distraction in the control group; in the experimental group, immature bone formation was observed adjacent to the host bone, and the collagen fibers were not arranged uniformly. At 8 week, spindle-shaped new bone formation was seen in the direction of distraction in distracted area of the control group, while in the experimental group, the newly formed bone was arranged in a multidirectional manner, like the pattern of trabeculae. In the histomorphometric analysis of 8 weeks, the area of bone deposition was $2.12{\pm}\;0.75\;inch^2$ in the experimental group and $0.87{\pm}0.51\;inch^2$ in the control group (p<0.01). The bone deposition ratio was $29.60{\pm}10.50%$ in the experimental group and $12.10{\pm}7.17%$ in the control group (p<0.01). Conclusion: These results suggest that compression after over-distraction during the mandibular distraction osteogenesis is an effective method of increasing the amount of newly formed bone in distracted area.

당뇨병 유발 토끼에서 발치 후 Hydroxyapatite 충전이 잔존 치조제 흡수에 미치는 영향 (THE EFFECT OF IMPLANTATION OF HYDROXYAPATITE IN THE FRESH EXTRACTION SOCKETS ON THE RESIDUAL RIDGE RESORPTION IN INDUCED DIABETIC RABBITS)

  • 배정식;정문규
    • 대한치과보철학회지
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    • 제30권4호
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    • pp.541-564
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    • 1992
  • The purpose of this experiment was to study the effect of implantation of the dense hydroxyapatite (HA) granules$(Apaceram^{(R)})$ in the fresh extraction sockets on the residual ridge resorption in normal and induced diabetic rabbits. Sixty-four white rabbits, weighing 3.0kg, were utilized in this study and they were divided into four group : group 1-sixteen normal rabbits without filling HA granules, group 2-sixteen normal rabbits with filling HA granules, group 3-sixteen diabetic rabbits without filling HA granules, group 4-sixteen diabetic rabbits with filling HA granules. HA granules were filled immediately after extraction in the extraction sockets of the mandibular left 1st, 2nd, and 3rd molar in group 2 and 4, and no HA granules were filled in group 1 and 3. The calculation of residual ridge resoption was done at 4th week, 8th week, 12th week, and 16th week after surgery, and histologic examination was performed to study healing process at 2nd week, 4th week, 8th week, and 16th week after surgery. The results were as follows : 1. The amount of residual ridge resorption was largest in group 3, and smallest in group 2 in every interval. There was no statistically significant difference between group 1 and 4. 2. In all groups, the amount of residual ridge resorption was high until 4th week after surgery, but the rate of residual ridge resolution slowly decreased after 4th week 3. There was more active healing process in group 1 and 2 than in group 2 and 4. 4. According to filling of HA granules, there was no difference in the amount of new bone formation, but direct contact could be observed between newly formed bone and HA granules in group 2 and 4. 5. Group 1 and 3 showed marked bone resorption and adipose marrow from 8th-16th week after surgery, but group 2 and 4 did not show maeked bone resolution, and showed fibrous tissue and a little adipose tissue among trabeculae from 8th week.

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압축력을 병용한 하악골 신장술 (MANDIBULAR DISTRACTION OSTEOGENESIS WITH COMPRESSION FORCE - BONE DENSITY, HISTOLOGICAL FINDINGS AND TMJ RESPONSE)

  • 황영섭;허준;김욱규;박성진;황대석;김용덕;정인교;김규천
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제28권6호
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    • pp.531-548
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    • 2006
  • The purpose of this study was to investigate the biomechanical, histologic findings of distracted regenerate and TMJ response in modified distraction osteogenesis (DO) technique combined with compression force as biomechanical stimulation method which has been suggested in 2002, and developed thereafter by authors. This study was performed with two experiments. First experiment was designed to explore the optimal ratio of compression force versus distraction force for the new DO technique. Second experiment was planned to evaluate the reaction of TMJ tissue, especially condyle, disc after application of the DO technique with compression force. Total 52 New Zealand adult male-rabbits with 3.0kg body weight were used for the study. For the first study, 30 adult male-rabbits underwent osteotomy at one side of mandibular body and a external distraction device was applied on each rabbit with same manner. In the control group of 10 rabbits, final 8 mm of distraction with 1 mm rate per day was done with conventional DO technique after 5 latency days. For the experimental group of 20 rabbits, a compression force with 1 mm rate per day was added to the distracted mandible on 3-latency day after over-distraction (over-lengthening). As the amount of the rate of compression versus distraction, experimental subgroup I (10 rabbits) was set up as 2 mm compression versus 10 mm distraction (1/5) and experimental subgroup II (10 rabbits) was set up as 3 mm compression versus 11 mm distraction (about 1/3). All 30 rabbits were set up to obtain final 8 mm distraction and sacrificed on postoperative 55 day to analysis on biomechanical, and histologic findings of the bone regenerates. For second study, 22 adult male-rabbits were used to evaluate TMJ response after the DO method application with compression force. In the control group, 10 rabbits was used to be performed with conventional DO method, on the other hand, in a experimental group of 10 rabbits, 10 mm distraction with 2 mm compression (1/5 ratio) was done. The remaining 2 rabbits served as the normal control group. Histomorphologic examinations on both condyle, histological studies on condyle, disc were done at 1, 2, 3, 4, 7 weeks after distraction force application. The results were as follows: 1. On the bone density findings, the experimental group II (force ratio - 1/3) showed higher bone density than the other experimental group (force ratio - 1/5) and control group (control group - $0,2906\;g/cm^2$, experimental group I - $0.2961\;g/cm^2$, experimental group II - $0.3328\;g/cm^2$). 2. In the histologic findings, more rapid bone maturation like as wide lamellar bone site, more trabeculae formation was observed in two experimental groups compared to the conventional DO control group. 3. In morphologic findings of condyle, there were no differences of size and architecture in the condyle in the control and experimental groups. 4. In histologic findings of condyles, there were thicker fiberous and proliferative layers in experimental group than those of control group until 2 weeks after distraction with compression force. But, no differences were seen between two groups on 3, 4, 7 weeks after compression. 5. In histologic findings of disc, more collagen contents in extracellular matrix, more regular fiber bundles, and less elastin fibers were seen in experimental group than control group until 2 weeks after distraction with compression. But, no differences were seen between two groups on 3, 4, 7 weeks after distraction with compression. From this study, we could identify that the new distraction osteogenesis technique with compression stimulation might improve the quality of bone regeneration. The no remarkable differences on TMJ response between control and experimental groups were seen and TMJ tissues were recovered similarly to normal TMJ condition after 3 weeks.

하악골의 발육중인 생쥐에서 기능력의 변화가 특이-유전자 발현에 미치는 영향 (THE EFFECT OF ALTERED FUNCTIONAL FORCE ON THE EXPRESSION OF SPECIFIC MRNAS IN THE DEVELOPING MOUSE MANDIBLE)

  • 김형태;박주철;이창섭;박헌동
    • 대한소아치과학회지
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    • 제30권2호
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    • pp.308-319
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    • 2003
  • 기능력은 뼈의 형성, 유지 및 개조 이외에도 치아이동, 교정치료, 기능성 악교정장치의 사용 등과 관련한 치주인대세포의 특성 변화 등에 중요한 영향을 미친다. 또한, 하악과두에서도 기능력의 변화에 따라서 다양한 특성의 변화가 나타난다. 본 연구에서는 ICR 생쥐를 8주 동안 soft-diet와 hard-diet의 식이 조절을 통해 기능력의 변화를 유도하여, 기능력의 변화와 관련한 특이 유전자를 검출하기 위하여 subtractive hybridization, northern 분석 및 mRNA in-situ hybidization 등의 실험을 시행하여 다음과 같은 결과를 얻었다. 1. Soft-diet군과 hard-diet군의 subtractive hybridization을 통하여 총 39개의 clone을 얻었고 이중에서 11개의 서로 다른 hard-diet군 특이 후보 유전자들을 분리하였다. 2. 11개의 후보 유전자들 중에서 homology 검색과 northern 분석을 통하여 기능력과 관련이 있을 것으로 생각되거나 hard-diet군에서 mRNA가 선택적으로 발현되는 FS-s2, FS-s5, FS-s18 및 Fs-s22 유전자를 선택하였다. 3. Soft-diet군과 hard-diet군의 형태학적 분석에서 soft-diet군은 hard-diet군에 비하여 골모세포의 활성과 골개조의 특성은 저하된 것으로 관찰되었으나, 하악과두의 연골성골화 과정은 오랜 시간동안 지속되는 것으로 나타났다. 4. FS-s2, FS-s5, FS-s18 및 Fs-s22 유전자의 cRNA 탐침자를 이용한 in-situ hybridization에서 4개의 유전자의 mRNA들은 hard-diet군의 세포들에서는 강하게 발현되었으나 soft-diet군의 세포들에서는 그 발현이 미약하거나 나타나지 않았다. 이상의 결과를 종합하여 FS-s2, FS-s5, FS-s18 및 Fs-s22 유전자들은 대부분이 기능이 거의 알려져 있지 않는 것들로 기능력의 변화 과정에서 중요한 역할을 할 것으로 생각되나 앞으로 보완 연구를 통하여 각 각의 유전자들의 보다 정확한 기능을 이해하는 것이 필요 할 것으로 사료된다.

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배자와 태아에서 하악골의 형태발생 및 교원질 발현에 관한 면역조직화학적 연구 (The Immunohistochemical Expression of Collagens and the Morphogenesis in the Developing Mandible of Human Embryos and Fetuses)

  • 국윤아;김상철;김은철;김오환;김정기
    • 대한치과교정학회지
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    • 제26권2호
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    • pp.187-196
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    • 1996
  • 치아 위치에 영향을 미치는 악안면의 성장 발육에서 Meckel 연골발생전 후의 하악골 형성과정과 교원 단백질 분포 및 발현 정도를 알아보고자 좌고를 측정하여 태령을 결정한 후 4주부터 38 주까지 50 례의 배자와 태아를 대상으로 통법에 따른 조직절편을 제작하였으며 Hematoxylin과 Eosin, Alcian blue-PAS와 Goldner의 Masson Trichrome 염색, 그리고 제 1 형과 제 2 형 교원 단백질에 대한 면역조직화학 염색을 시행하였다. 좌고 20.5 mm 배자에서 Meckel 연골이 출현하였으며, 좌고 22 mm에서 38 mm까지 하악골 외방에 신생골을 형성하고, 좌고 60 mm태아에서 Meckel 연골이 점유하던 공간이 신생골로 채워져 연골내골화가 뚜렸하게 관찰되었으나, 좌고 240 mm에서 Meckel 연골이 거의 소실되었다. 교원질에 대한 면역 염색결과에서 Meckel 연골 출현전 제 1 형 교원질 발현은 주로 상, 하악돌기의 구강상피에 국한되어 관찰되었고 제 2 형 교원질 발현은 상대적으로 약간 적었다. Meckel 연골 출현 및 신생골 형성시기는 제 1 형 교원질이 주로 치제상피와 신생골에서 약양성의 발현을 보였으며 Meckel 연골 및 신생골에서는 제 1 형보다 제 2 형의 교원질이 많이 발현되었다. 막내골화시기에는 제 1 형 교원질이 골아세포 및 골기질에서 중등도로 발현되었으나, 제 2 형에서는 경미하게 나타나 Meckel 연골형성전 후 제 2형에서 제 1 형으로 발현 전환이 있었다.

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