• 제목/요약/키워드: toxigenic pattern

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닭에서 분리한 Clostridium perfringens의 독소형 (Toxigenic type of Clostridium perfringens isolated from chicken in Korea)

  • 박경윤;이상운;유한상;예재길
    • 대한수의학회지
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    • 제36권4호
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    • pp.829-837
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    • 1996
  • Clostridium perfringens has been identified as a causative organism in necrotic enteritis in chicken. The bacterium has been classified into five toxigenic types (A through E) based on the pattern of the production of major lethal toxins. Seroneutralization with mice or guinea pigs usually has been used to type the organism. Of the types, types A and C of the bacterium had been recognized as the major pathogenic types in chicken. In this experiment, we isolated nine field strains of C perfringens from chicken showing necrotic enteritis in clinical symptoms and pathologic findings and identified by biochemical tests. In order to type the organism, a multiplex polymerase chain reaction (PCR) was used with primers on major lethal toxin genes instead of seroneutralization. Amplification of only a toxin gene with the PCR suggested that the disease in chicken was due to type A of C perfringens in Korea. Furthermore, the PCR method can be replaced with seroneutralization to type C perfringens in future.

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Toxin Gene Profiling of Bacillus cereus Food Isolates by PCR

  • Seong, Seon-Je;Lim, Ji-Su;Lee, Kwang-Geun;Lee, Seung-Ju;Hong, Kwang-Won
    • Applied Biological Chemistry
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    • 제51권4호
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    • pp.263-268
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    • 2008
  • Seventy-one Bacillus cereus strains (12 references and 59 food isolates) were analyzed for the occurrence of five different enterotoxin genes (nheABC, hblCDA, entFM, cytK, and bceT) and one emetic toxin cereulide synthetase gene (ces) by PCR (polymerase chain reaction). PCR analysis revealed eight toxigenic patterns in all B. cereus strains tested; they all carried both entFM and nheABC. The presence of hblCDA, cytK, and bceT varied according to the enterotoxin-producing strains, among which hblCDA was the least frequently detected in the food-isolated strains. Only five B. cereus strains harbored ces, associated with the emetic type of food poisoning; however, these strains were devoid of hblCDA, cytK, and bceT.

서울지방에서 분리된 Corynebacterium diphtheriae 균주에 관한 연구 (Studies on the Toxigenic Strains of Corynebacterium diphtheriae Isolated in Seoul Area)

  • 신용우;장우현
    • 대한미생물학회지
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    • 제8권1호
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    • pp.13-17
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    • 1973
  • To understand the characteristics of 29 toxigenic strains of Corynebacterium diphtheriae isolated in Seoul area, type classification, biochemical properties and antibiotic susceptibility pattern to 9 kinds of antibiotics were investigated. The results obtained were summerized as follows; I. Among the 29 strains, gravis type was the overwhelming majority(24 strains), followed by intermedius type(3 strains) and mitis type(2 strains). II. Fermentation of glucose, maltose, lactose, trehalose and mannitol, nitrate reduction and urease were tested. All strains fermented glucose, but not sucrose, lactose, mannitol and trehalose. 9 strains fermented maltose and 20 strains did not. Nitrate was reduced by 28 strains but not by one strain. In urease test one strain showed positive, 28 strains negative. III. Antibiotic susceptibility test to penicillin G, chloramphenical, kanamycin, lincomycin, streptomycin, terramycin, erythromycin and gentamycin were carried out. The MIC of erythromycin(0.025 ${\mu}g/ml$ 26 strains and 0.05 ${\mu}g/ml$ 3 strains) was the lowest, followed by ampicillin, lincomycin and penicillin G. Streptomycin showed the highest MIC.

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