• Herbal Formula Science
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• v.29 no.4
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• pp.239-252
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• 2021

Objectives : This study is to effect of improving muscle atrophy through water extract on the solid-phase fermentation extraction with Phellinus linteus of discarded Schisandra chinensis in an atorvastatin-induced atrophy C2C12 cell. Methods : C2C12 myoblast were differentiated into myotube by 2% horse serum medium for 6 days, and then treated solid-phase fermentation(S-P) extract at different concentrations for 24h. To investigate the effect of S-P extract on the induction of muscle atrophy and expression of atrophy-related genes and apoptosis in differentiated C2C12 myotubes using a GSH, ROS, real-time PCR, western blots analysis. Results : As a result of treatment with atorvastatin at concentrations of 5, 10, and 20 uM on the 6th day of differentiation in C2C12 myotube cells, it was confirmed that the cell morphology was damaged in a concentration-dependent manner, and the length and thickness of the myotube also decreased in a concentration-dependent manner. Treatment with S-P extract (50, 100 and 200 ㎍/㎖) increased of GSH and inhibited ROS in the atorvastatin-induced muscle atrophy cell model at a concentration that did not induce toxicity. In addition, it was confirmed that it has an effect on muscle reduction by inhibiting apoptosis of muscle cells as well as being involved in protein production and degradation of muscle cells. Conclusions : Atorvastatin-induced atrophy C2C12 cell, S-P extract activates related to differentiation/generation and proteolysis, and inhibits cell death of atrophy in C2C12 cell. Based on this, it is necessary to prove its effectiveness through animal models and human application test, but it is considered to be discarded Schisandra chinensis can present the potential for development as a recycling industrial material.

• Applied Chemistry for Engineering
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• v.29 no.6
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• pp.690-695
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• 2018

Advanced oxidation processes (AOPs) composed of O3 and UV were applied to degrade penicillin (PEN). The degradation efficiency was evaluated in terms of changes in the absorbance (ABS) and total organic carbon (TOC). The combination of $O_3/H_2O_2/UV$ and $O_3/UV$ showed the best performance for the reduction of ABS (100% for 9 min) and TOC (70% for 60 min) values, although the mineralization was uncompleted under the experimental condition in this study. The change in biotoxicy was monitored with Escherichia coli susceptibility and Vibrio fischeri biofluorescence. The E. coli susceptibility was eliminated completely for 9 min by $O_3/UV$, and the toxicity to V. fischeri biofluorescence was 57% reduced by $O_3/H_2O_2/UV$. For the ultimate treatment of PEN, it is suggested that an AOP using $O_3/UV$ is followed by biological treatment, utilizing the enhanced biodegradability by the AOP. During 30 min of $O_3/UV$ treatment, the $BOD_5/COD$ ratio as an indication of biodegradability showed about 4-fold increment, compared to that of using a non-treated sample. TOC removal rate for AOP-pretreated PEN wastewater increased 55% compared to that of using the non-pretreated one through an aerobic biological treatment by Pseudomonas putida for artificial wastewater containing 20 mg/L of PEN. In conclusion, $O_3/UV$ process is recommended as a pretreatment step prior to an aerobic biological process to improve the ultimate degradation of penicillin.

• Korean Journal of Food Science and Technology
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• v.50 no.6
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• pp.697-700
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• 2018

This study investigated changes in amygdalin of maesil liqueurs during leaching and ripening, and their qualities during ripening. Maesil was leached at $24^{\circ}C$ for 3 months in a commercial soju (30% alcohol). During leaching, amygdalin contents dramatically increased by 1 month, remained at a plateau by 2 months, and then decreased at 3 months. Once completion of leaching, maesil liqueurs were ripened for 3 months together with maesil (ML1) and maesil flesh itself (ML2), and without maesil (ML3). During ripening, amygdalin contents significantly decreased for all treatments, and the reduction in amygdalin rapidly proceeded in the order: ML2>ML1>ML3. Alcohol contents decreased for ML1 and ML2, while remained constant for ML3. Their pH and titratable acidity observed only subtle changes, and their color became increasingly darker. Overall results suggested that the long-term ripening of maesil liqueurs containing maesil or maesil flesh (without seeds) may resolve the concern on their amygdalin toxicity.

• Economic and Environmental Geology
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• v.53 no.6
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• pp.781-792
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• 2020

Mine tailings, which are inevitably formed by the development of manganese nodules, manganese crusts, and hydrothermal seafloor deposits, have attracted attention because of their quantity and potential toxicity. However, there is a lack of data on the quantity of mine tailings being generated, their physicochemical properties, and their effects as environmental hazards and on marine ecosystems in general. The importance of treating mine tailings in an environmentally friendly manner has been recognized recently and related reduction/treatment methods are being considered. In the case of deep-sea mineral resource development, if mine tailings cannot be treated aboard a ship, the issue becomes one of the cost of transporting them to land and solving the problem of environmental pollution there. Therefore, the Korea Institute of Ocean Science and Technology conducted research on the harmfulness of mine tailings, their effect on marine ecosystem, the diffusion model of contaminated particles, and candidate purification treatment technologies based on five representative controlling factors: 1) effects of pollution /on the environment, 2) effects of environmental/ biological hazards, 3) diffusion of particles, 4) mineral dressings, and 5) reducing/processing mine tailings. The results of this study can provide a basis for minimizing environmental problems by providing scientific evidences of the environmental effects of mine tailings. In addition, it is also expected that these results could be applied to the treatment of pollutants of different origins and at land-based mining waste sites.

• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.118-125
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• 2021

In this study Orostachys japonica A. Berger used is a medicinal herb that has long been used as a folk remedy for cancer treatment. In this study, the antioxidant efficacy of the ethyl acetate fraction of Orostachys japonica A. Berger was confirmed. The results of the Orostachys japonica A. Berge ethyl acetate fraction of antioxidant activity assays showed Antioxidant effect of Orostachys japonica A. Berger EtOAc fraction extract at 0.10 mg/mL was showed a DPPH radical scavenging rate of 78.54% and ABTS+ radical scavenging rate of 73.48%. Also, the toxicity result of Orostachys japonica A. Berger EtOAc fraction extracts using alternative experimental animal model zebrafish, confirmed a 100% the survival of the zebrafish embryo was shown that there was no coagulation and no hatching delay at all concentrations. also ROS generation induced by UV-B irradiation was confirmed that the fluorescence intensity decreased as a whole in all larvae treated with Orostachys japonica A. Berger EtOAc fraction extracts. In particular, it was confirmed that ROS generation was effectively suppressed by showing a 35.7% reduction rate compared to the positive control at a concentration of 3 ㎍/mL. These results were confirmed that Orostachys japonica A. Berger EtOAc fraction extracts has the possibility of application in the cosmetics field as a natural antioxidant.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.255-266
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• 2022

This study was conducted to examine the effects of grass vegetation (W: manual weeding, NW: herbicide sprays) and pyrethroid spray (P: pyrethroid spray, NP: no pyrethroid spray) on the population dynamics of Panonychus citri and natural enemies in citrus orchards. Two essential hypothesis were made to test the population dynamics: 1) weed planting promotes natural enemies by offering habitat and alternative food sources, resulting in the reduction of P. citri populations, and 2) pyrethroid spray removes natural enemies by its non-selective toxicity, resulting in the increasement of P. citri populations. The observed natural enemy populations (mainly Phytoseiids and Agistemus sp.) were not different largely from the expected values in the hypothesis, which assumes more abundant natural enemies in weeds and no pyrethroid plots. Although some discrepancy was occurred in NW+NP and W+NP plots in 2011, the observed values were almost same with expected values in 2012. In overall, pesticide effect was strongly significant and pyrthroids removed largely natural enemies. Although habitat (weeds) effect showed a conflict result, natural enemy population increased in plots allowing weed growth, when considering the increased autumn population relatively compared to that of spring-summer population. The decreased abnormal P. citri populations in pyrethroid plots could be explained under the assumption of a strong repellent behavior of P. citri to the pyrethroids.

• Journal of Soil and Groundwater Environment
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• v.27 no.4
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• pp.10-21
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• 2022

In the South Korea, 47% of abandoned mines are suffering from the mining hazards such as the mine drainage (MD), the mine tailings and the waste rocks. Among them the mine drainage which has a low pH and the high concentration of heavy metals can directly contaminate rivers or soil and cause serious damages to human health. The natural/artificial treatment facilities by using neutralizers and coagulants for the mine drainage have been operated in domestic and most of heavy metals in mind drainage are precipitated and removed in the form of metal hydroxide, alumino-silicate or carbonate, generating a large amount of mine drainage treated sludge ('MDS' hereafter) by-product. The MDS has a large surface area and many functional groups, showing high efficiency on the fixation of heavy metals. The purpose of this study is to develop a ingenious heavy metal stabilizer that can effectively stabilize arsenic (As) and heavy metals in soil by recycling the MDS (two types of MDS: the acid mine drainage treated sludge (MMDS) and the coal mine drainage treated sludge (CMDS)). Various analyses, toxicity evaluations, and leaching reduction batch experiments were performed to identify the characteristics of MDS as the stabilizer for soils contaminated with As and heavy metals. As a result of batch experiments, the Pb stabilization efficiency of both of MDSs for soil A was higher than 90% and their Zn stabilization efficiencies were higher than 70%. In the case of soil B and C, which were contaminated with As, their As stabilization efficiencies were higher than 80%. Experimental results suggested that both of MDSs could be successfully applied for the As and heavy metal contaminated soil as the soil stabilizer, because of their low unit price and high stabilization efficiency for As and hevry metals.

• Journal of Aerospace System Engineering
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• v.17 no.6
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• pp.1-8
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• 2023

Research, such as developing alternative energy in the transportation field, including aviation, is being actively conducted to solve the issue of current climate change. Interest in ammonia fuel as a carbon free energy (CFE) source is increasing due to the ease of liquefaction and transportation and similarity in energy density to that of methanol. However, explosiveness and toxicity of ammonia make it difficult to handle. Therefore, in this study, stable ammonia production was attempted using relatively easy-to-handle urea water solution (UWS). High temperature steam was used to promote the hydrolysis of ammonia. In order to determine the causes for ammonia production below the theoretical equivalent ratio, it was suggested that there were not enough collisions to promote the hydrolysis based on the kinetic theory of gases. The hydrolysis of unreacted isocyanic acid (HNCO) was tested according to the change in water supply. As a result, an increased amount of ammonia produced was confirmed. The increased amount of ammonia produced in a certain section was dependent on the steam temperature and the flow rate of water supplied.

• Journal of Environmental Impact Assessment
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• v.32 no.5
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• pp.291-304
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• 2023

Harmful algal blooms (HABs) have become an increasing concern in terms of human health risks as well as aesthetic impairment due to their toxicity. The reduction of water pollutants, especially nutrients from non-point sources in a reservoir watershed, is fundamental for HABs prevention. We investigated the pollutant removal efficiencies of a constructed wetland to evaluate its feasibility as a method for controlling non-point sources located in the Annaecheon stream within the Daecheong Reservoir watershed. The overall removal efficiencies of pollutants were as follows: BOD 14.3%, COD 17.9%, SS 50.0%, T-N 19.0%, and T-P 35.4%. These results indicate that constructed wetlands are effective in controlling pollutants from non-point sources. The seasonal variation in removal efficiency depended on the specific pollutants. The removal efficiencies of BOD, COD, and T-N were stable throughout the year, except during winter, which might have been influenced by lower microorganism activity. In contrast, T-P showed a consistent removal efficiency even during the winter season, suggesting that the wetland can reduce external phosphorus loading to the reservoir. Regarding the effects of pollutant loadings on removal efficiency, the effluent concentrations of all pollutants were significantly decreased compared to those in the influent in case of middle and high loadings. This demonstrates that constructed wetlands can handle high pollutant loads, including the initial runoff during rainfall, to prevent reservoir eutrophication. Despite the various strengths of wetland water purification, there are limitations as passive treatment. Therefore, more case studies should be conducted to suggest optimum operational conditions for constructed wetlands, taking into consideration reservoir-specific characteristics.

• Journal of the Society of Disaster Information
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• v.20 no.1
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• pp.93-105
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• 2024

Purpose: This study aims to quantitatively determine the impact on nearby risidents by selecting the amount of chemicals emitted from the workplace among the substances subject to the chemical emission plan and predicting the concentration with the atmospheric diffusion program. Method: The selection of research materials considered half-life, toxicity, and the presence or absence of available monitoring station data. The areas discharged from the materials to be studied were selected as the areas to be studied, and four areas with floating populations were selected to evaluate health risks. Result: AERMOD was executed after conducting terrain and meteorological processing to obtain predicted concentrations. The health hazard assessment results indicated that only dichloromethane exceeded the threshold for children, while tetrachloroethylene and chloroform appeared at levels that cannot be ignored for both children and adults. Conclusion: Currently, in the domestic context, health hazard assessments are conducted based on the regulations outlined in the "Environmental Health Act" where if the hazard index exceeds a certain threshold, it is considered to pose a health risk. The anticipated expansion of the list of substances subject to the chemical discharge plan to 415 types by 2030 suggests the need for efficient management within workplaces. In instances where the hazard index surpasses the threshold in health hazard assessments, it is judged that effective chemical management can be achieved by prioritizing based on considerations of background concentration and predicted concentration through atmospheric dispersion modeling.