• 한국수산과학회지
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• 제54권6호
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• pp.841-848
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• 2021

To develop a value-added low-salt fermented seafood with a long shelf-life, we prepared seasoned low-salt fermented Omandungi Styela plicata supplemented with fermentation alcohol (SOE). The SOE was produced by washing and dewatering shelled Omandungi, followed by cutting and salting for 24 h at 0±1℃. The salted Omandungi was seasoned and fermented with garlic, ginger, monosodium glutamate, red pepper, sesame, sorbitol, and sugar, for 7-8 days at 0±1℃. After adding 3-5% fermentation alcohol, the seasoned fermented Omandungi was packed in a polyester container. The salinity, volatile basic nitrogen content, and viable cell count of SOE were 4.8%, 22.1-22.2 mg/100 g, and (1.2-1.9)×10³ CFU/g, respectively. Compared with the control, addition of 3-5% fermentation alcohol inhibited the decrease in freshness, texture degradation, and growth of residual bacteria. Additionally, the SOE showed good storage stability and organoleptic qualities when stored at 4±1℃ for 40 days. The total amino acid content of SOE was 2,186.0 mg/100 g, mainly comprising glutamic acid, aspartic acid, lysine, and phenylalanine. The free amino acid content was 189.0 mg/100 g, and mainly included taurine, glutamic acid, methionine, alanine, and proline.

• 한국수산과학회지
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• 제55권4호
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• pp.437-442
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• 2022

Squid jeotgal is a very popular traditional, salted, and fermented seafood in Korea. Due to the development of a distribution structure, a lot of imported squid jeotgal has recently entered Korea. In this study, we analyzed and compared the microbial contamination levels of domestic and imported squid jeotgal. The total viable bacterial count in domestic and imported jeotgal was 5.20 and 5.38 log CFU/g, respectively. The highest contamination level was 6.94 log CFU/g in one of the imported squid jeotgal samples. Coliforms were not detected in domestic jeotgal but were detected at 50% with 2.21 log CFU/g in imported jeotgal. Escherichia coli and Staphylococcus aureus were not detected (ND: <1 log CFU/g) in both domestic and imported jeotgal. All samples of jeotgal were negative for Bacillus cereus and Clostridium perfringens. The microbial contamination levels determined in the current study may be utilized as primary data to execute microbial risk assessments on domestic and (or) imported jeotgal.

• 한국미생물·생명공학회지
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• 제50권3호
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• pp.387-394
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• 2022

Verotoxin-1 (VT-1) or Shiga-like toxin 1 (Stx-1) is produced by enterohemorrhagic Escherichia coli (EHEC) and is an AB5 holotoxin with a strong inhibitor of protein synthesis. VT-1 is a type 2 ribosome-inactivating protein (RIP) that has been shown to have cytotoxic and anticancer potential by inducing necrosis, apoptosis, and cell cycle arrest, making it a promising antitumor candidate. Here, we tested the cytotoxicity of VT-1 on CaCo2 and NCM425 cell lines and the results showed that VT-1 was more potent on CaCo2. Morphological changes were also evaluated on the cellular level and the results showed that VT-1 caused a decrease in viable cell count, altered cell membrane permeability, and an increase in total nuclear intensity. On the other hand, VT-1 displayed a lesser impact on mitochondrial membrane potential (MMP) and cytochrome c release. On the expression of caspases 3 and 9, VT-1 exhibited an insignificant effect on both which alongside the mitochondrial membrane potential (MMP) and cytochrome c results, might indicate that CaCo2 suffered from the necrosis process as a mechanism of cell death after exposure to VT-1.

• 한국환경과학회지
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• 제10권5호
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• pp.365-371
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• 2001

The optimum dosage of quicklime in producing organic fertilizer using livestock wastes vith a greater than 80% water content was analysed. After one day had elapsed to allow for the organic fentilizer to dry, the quicklime dosage and the composition of the organic fertilizer were analysed. Any from done to the organic fertilizer was also assessed. The amount of the quicklime required to stabilize livestock wastes was determined by water content of livestock wastes. For J farm(slurry style) of which livestock wastes have 94.6% of water concentration, less than 3% of total amount of livestock wastes, for H farm (scraper style) of which livestock wastes have 85% of water concentration, less then 4% of total livestock wastes and Y farm(traditional style) of which livestock wastes have 80% of water concentration, less then 5% of total livestock wastes. Generally, in order to pack the organic fertilizer, water containing quicklime0stabilized livestock wastes should be less than 35%. It takes 9 days to keep this water content for the wastes from H and Y farms(less than 85% in water content), and 12 days for the wastes from J (94.6% in water content). According to the classification standard for compost constitution by Higgins, the crude fertilizers from all 3 farms had high grade $K_2O$ and CaO, the middle grade T-N and middle or low grade $P_2O_5$. Stabilization by quicklime is known to inhibit bacterial decomposition of organic matter and the activity of pathogenic organisms. In this study, more then 99.99% of coliform group, fecal group and viable cell count were reduced. Our results indicate that livestock wastes of greater 80% water content could be used to produce organic fertilizer without the addition of a material for moisture control.

• Nuclear Engineering and Technology
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• 제1권1호
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• pp.17-22
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• 1969

English sole(가재미종) 어편을 Polymylar bag에 밀봉하여 500 Krad의 감마선에 조사한 후 36일간의 0-2$^{\circ}C$ 저장기간중 조사한 것과 조사하지 않은 어편내에 축적되는 부패표시 물질과 총 균수를 측정하였다. 단백질 분해균을 직접 계수하기 위하여 Casein agar Plate 법을 만들어 저장기간중의 총균수비 대한 단백질분해균의 비율을 측정하는데 사용하였다. 500 Krad 선량 조사결과 어편의 총 균수는 10분의 1로 감축되었고 저장중 조사하지 않은 어편의 총균수 보다 훨씬 적어 1000분의 1선까지 내려갔다. 이에 따라 TVB와 TMA 축적도 억제되어 부패선을 넘지 못했으며 단백질 분해균 역시 총균수의 1% 이하선으로 감축되었으며 저장 기간중 이 선을 넘지 못했다. 반면에 조사하지 않은 어편엔 총 균수에 대한 이들의 비율은 저장기간중 점차 상승하여 22일째까지 총균수의 85.5%를 차지하였다. 방사선 조사로 인한 이 단백질 분해균의 선택적인 제거는 그 어편의 단백질 분해 부진 현상을 초래했다.

• 한국동물위생학회지
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• 제40권1호
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• pp.47-52
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• 2017

The main goal of this survey was to assess the current sanitation status and safety standards of meat processed products purchased at instant meat sales and processing operators. Analyses were carried out from April to September in 2016 in Gwangju area, Republic of Korea. A total number of 150 samples including seasoned meats, ground meat products, meat extract products, heated seasoned meats, sausages and hams from 35 butcher shops was collected. The number of inappropriate cases was revealed eleven cases (7.3%) in total viable count of bacteria (TVC), total coliform counts (TCC) and Listeria monocytogenes. The reported data indicate that more systemic and technical guidance is needed to monitor instant meat sales and processing operators in order to guarantee safety of meat processed products.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.67-72
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• 1993

Three sheep fitted with cannulas in the rumen and the caecum were used in a $3{\times}3$ Latin square design to study the changes in ruminal and caecal microbial populations and their metabolite composition with ammoniated rice straw feeding. The 3 diets contained either 80% untreated rice straw (UTS) or ammoniated rice straw (ATS) and 20% formula feed. These were a control diet (C), a urea supplemented diet (U) containing urea at 1.1% and an ammoniated rice straw diet (AT). Data were analyzed by analysis of variance and means separated by the Student Neumann Kuel's multiple comparison. AT feeding increased ruminal bacterial counts, in particular cellulolytic bacterial counts (p < 0.05) which were 1.8, 2.4 and 7.0 (${\times}10^6/ml$ ruminal fluid) for C, U and AT, respectively. There was an increasing tendency (p < 0.10) in ruminal fungal population with U; values were 2.0, 5.2, 3.1 (${\times}10^3/ml$ ruminal fluid) for C, U and AT, respectively. Ruminal protozoa counts were not significantly (p > 0.05) altered with diets. Caecal total viable bacterial count with AT was about thrice the value with C. Total VFA concentration in the rumen was significantly increased (p < 0.025) (7.7 mmol/dl for C and 8.2 mmol/dl for AT) and correspondingly, pH lowered when AT was fed. Sheep on AT tended to produce less acetate and more butyrate in the rumen without significance (p > 0.05). Similar to the rumen, total VFA concentrations of 4.4, 3.8 and 5.2 mmol/dl were detected, respectively, for C, U and AT. Caecal ammonia-nitrogen concentrations were about six-fold of that in the rumen, though there were no differences (p > 0.05) among treatments.

• 한국식품영양학회지
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• 제16권4호
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• pp.353-358
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• 2003

우리나라의 전통적인 젓갈류는 염도가 지나치게 높고 제조 방법이 단순하고 비위생적으로 생산되는 경우가 많아 고품질 젓갈 제품의 생산을 위해서는 새로운 공정 개발이 필요한 실정이다. 따라서 본 연구는 명란을 액 염지법으로 염도를 8%로 줄이고 진공 숙성기법으로 맛을 강화시켜 새로운 명란 젓갈을 제조하였다. 또한 저염 젓갈의 저장성을 높이기 위하여 진공 발효시켜 저장하면서 숙성 형태별 숙성 기간에 따른 이화학적 성분의 변화를 조사한 결과 명란 젓갈의 수분, pH 및 염도 변화는 숙성 기간이 경과함에 따라 큰 차이 없이 감소하는 경향이었고 수분은 무진공(상압) 숙성시에 약간 더 감소하는 것으로 나타났으며 휘발성 염기 질소(VBN) 및 구성 아미노산의 함량은 숙성형태에 상관없이 숙성이 진행됨에 따라 약간씩 증가하였고 VBN의 함량이 상압숙성에서 높은 경향이었고 총 구성아미노산은 진공 숙성시에 더 많게 나타났다. 총균수 역시 어느 정도 증가했다가 다소 감소하는 경향은 비슷하나 진공 숙성에 비해 상압 숙성시에 높았다. 이상의 결과로 볼 때, 명란젓갈의 숙성시 진공처리는 전반적인 젓갈의 숙성을 지연시켜주며 젓갈의 품질 유지 기간에도 효과적으로 나타났다.

• Applied Biological Chemistry
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• 제32권3호
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• pp.286-294
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• 1989

가열처리 및 건조방법이 새우의 품질에 미치는 영향을 밝히고자 하였다. 생새우의 콜레스테롤 함량은 건물 100g당 81.4mg이었으며, 동결 건조한 것은 열풍건조한 것보다 그의 함량이 $5{\sim}28%$가 적었다. 새우의 신선도와 관계가 있는 volatile basic nitrogen과 trimethylamine의 함량은 열풍건조한 것이 동결건조한 것보다 이들의 함량이 높았고 세균수는 동결건조한 것이 열풍건조한 것보다 낮았다. 생새우를 가열하거나 건조하면 L값이 크게 상승하여 새우의 색깔이 밝아졌는데 동결건조한 것은 열풍건조한 것보다 그 폭이 더 컸고 색차는 L값과 반대현상을 나타냈다. 생새우의 무기질 중에는 칼슘 함량이 가장 많았고 그 다음은 칼륨, 나트륨이었으며, 이들 무기질 성분은 전체 무기질 성분의 96%를 차지하였으며 가열 및 건조방법에 따라 차이가 없었다.

• 한국식품과학회지
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• 제28권1호
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• pp.137-145
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• 1996

기업적으로 생산하는 공장의 김치를 시료로 하여 5, 15, $25^{\circ}C$에서 발효하면서 화학적 성분과 함께 젖산균과 효모의 변화를 관찰하였다. 발효온도에 따른 숙성김치의 적정 pH인 4.2에 도달하는 시간은 $25^{\circ}C$의 경우 2일, $15^{\circ}C$의 경우 3일, $5^{\circ}C$의 경우 23일 소요되었다. 젖산량으로 산출한 최적 산도는 pH와 정확하게 일치하지는 않았다. 총균수는 발효온도 $25^{\circ}C$의 경우 2일, $15^{\circ}C$는 6일, $5^{\circ}C$는 12일에 최고 수준에 달하였다. 김치 발효동안 동정된 Lactobacilli 종은 L. brevis, L. plantarum, L. acidophilus와 3종의 미확인 균종이었다. L. brevis, L. plantarum은 $15^{\circ}C$, $25^{\circ}C$ 발효 온도에서 발효 시작에서부터 종결까지 일정수준을 유지하면서 나타났다. Leuconostoc종은 L. mesenteroides subsp. mesenteroides로 동정되었다. L. mesenteroides subsp. mesenteroides는 발효 초기에는 균수가 증가하였다가 모든 발효온도에서 발효 후기에는 급격하게 감소하였다. Pediococcus종은 P. pentosaceus와 1종의 미확인종으로 밝혀졌으며 발효 초기에는 증가하였다가 후에 감소하였다. 발효 중기에 나타난 Streptococcus faecium subsp. casseliflavus와 Aerococcus viridans 또한 김치 발효중에 동정된 균이다. Crytococcus laurenti는 모든 발효온도에서 동정되었고 발효초기에 나타난 후 사라졌다가 $25^{\circ}C$ 발효온도에서만 10일 후에 다시 나타났다.