• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.812-822
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• 2014

Metagenomic analysis of the human intestinal microbiota has extended our understanding of the role of these bacteria in improving human intestinal health; however, a number of reports have shown that current total fecal DNA extraction methods and 16S rRNA universal primer sets could affect the species coverage and resolution of these analyses. Here, we improved the extraction method for total DNA from human fecal samples by optimization of the lysis buffer, boiling time (10 min), and bead-beating time (0 min). In addition, we developed a new long-range 16S rRNA universal PCR primer set targeting the V6 to V9 regions with a 580 bp DNA product length. This new 16S rRNA primer set was evaluated by comparison with two previously developed 16S rRNA universal primer sets and showed high species coverage and resolution. The optimized total fecal DNA extraction method and newly designed long-range 16S rRNA universal primer set will be useful for the highly accurate metagenomic analysis of adult and infant intestinal microbiota with minimization of any bias.

• The Korean Journal of Ecology
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• v.8 no.2
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• pp.119-126
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• 1985

One nest of the Common Kingfisher Alcedo atthis bengalensis Gmelin breeding in the area of Mt. Bangu, Mugye-ri, Jangye-ri, Jangyu-myon, Gimhae-gun, Gyongnam province, was observed for 43 days from July 18 to August 29, 1984. The nest was found at a height of 71cm in a steep mountain side. It was tunnel-shaped, with a size of 53cm(length), 5.5*6.4cm (the diameter of entrance) and 16.1*10.5cm (the diameter of incubation seat). The cluth size was 5 eggs. The eggs were white in color, the average size of 5 eggs being 20mm (length)*17.9mm(width). The incubation period was 18 days, the parent birds stayed in the nest at on average of 494 minutes and the rate of incubation was 82.20%. The incubation time increased gradually. The nesting period of 3 chicks was 25 days, and the average feeding frequency was 5, 50 times per day with the highest frequency of 10 times on the 14th days after hatching. The time and frequency of attendance gradually decreased as their chicks grew up. Total increase length in body was; body length 45.35longrightarrow147.80mm, wing 6.95longrightarrow63.40mm, bill 1.73longrightarrow29.36mm and body weight 2.93longrightarrow41.00gr. The prey for the 3 chicks of the nest was entirely fish. The main food item was the fry of Gobiobotidae. The home range of the nest was 0.43ha. and the territory size was 0.05ha. Their feeding ground were outside of their territory.

• Journal of Astronomy and Space Sciences
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• v.31 no.2
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• pp.145-148
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• 2014

There was a research on the prolongation of solar cycle 23 by the solar cyclic variation of solar, interplanetary geomagnetic parameters by Oh & Kim (2013). They also suggested that the sunspot number cannot typically explain the variation of total solar irradiance any more. Instead of the sunspot number, a new index is introduced to explain the degree of solar activity. We have analyzed the frequency of sunspot appearance, the length of solar cycle, and the rise time to a solar maximum as the characteristics of solar cycle. Then, we have examined the predictability of solar activity by the characteristics of preceding solar cycle. We have also investigated the hemispheric variation of flare index for the periods that the leading sunspot has the same magnetic polarity. As a result, it was found that there was a good correlation between the length of preceding solar cycle and spotless days. When the length of preceding solar cycle gets longer, the spotless days increase. It is also shown that the shorter rise time to a solar maximum is highly correlated with the increase of sunspots at a solar maximum. Therefore, the appearance frequency of spotless days and the length of solar cycle are more significant than the general sunspot number as an index of declining solar activity. Additionally, the activity of flares leads in the northern hemisphere and is stronger in the hemisphere with leading sunspots in positive polarity than in the hemisphere with leading sunspots in negative polarity. This result suggests that it is necessary to analyze the magnetic polarity's effect on the flares and to interpret the period from the solar maximum to solar maximum as the definition of solar cycle.

• Journal of Forest and Environmental Science
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• v.32 no.2
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• pp.189-195
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• 2016

Logging equipment and method have a major influence on harvesting productivity and cost. This study investigated the productivity and operational costs of tree-length cable yarding system using HAM300, a domestically developed tower yarder. We tested HAM300 for thinning operation in Pinus densiflora stands at Gangreung, Gangwon-do on April, 2014. To assess the productivity we conducted time study for each stage of the operation. When the average time/cycle was examined for each stage of the operation, the longest was for yarding (241 sec), followed by delimbing (237 sec), felling (153 sec), and processing (103 sec). Furthermore, productivity for felling was $8.6m^3/hr$, followed by delimbing ($5.1m^3/hr$), yarding ($3.5m^3/hr$), and processing ($8.1m^3/hr$). The total cost for the tree-length logging system was $58,446won/m^3$, of which the majority was incurred by the yarding cost at $46,217won/m^3$ (79.3%), whereas the lowest cost was for felling at $2,359won/m^3$ (4.1%). We suggest that it is necessary to foster specialized operators and provide training in operating the tower yarder thereby implementing efficient harvesting system resulting from low-cost yarding.

• The Korean Journal of Emergency Medical Services
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• v.1 no.1
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• pp.42-53
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• 1997

To offer basic data about the influential factors on patient's Satisfaction level for emergency medical services the authors were performed this study in 60 patients visited to emergency room of third stage general hospital in Taejeon city. Data were collected through interview with patients by using a 15 items' questionaire according to care subscale, teaching subscale of Likert's five stage quantitative scale and the tools developed by Barbara Davis. The data were analyzed by using the SPSS/PC computerized program for mean, standard deviation, percentage, ANOVA, t-test, and pearson correlation. The results are as followings ; 1. Satisfaction of emergency medical service were showed in care area, but not showed in teaching and total area 2. Satisfaction of sociodemographic characteristics were a statistically significant difference only marriage, that were the higher in marriaged than single(P<.0.05). 3. In the emergency situation characteristics the satisfaction accordings to the visit cause were lower in accident than disease group, the reason of hospital selection were in order trust, introduction group, distance, traffic, kinds, and score of satisfaction were showed each of 43.47, 51.27(P<.001), the transportation vehicle was the 119, hospital ambulance group. but were not a satistically significant difference. 4. The negative correlation was observed between satisfaction and the length of stay at ER and the longer length of stay at ER was showed the lower satisfaction. But the positive correlation was observed between satisfacton and the arrival time, patients who arrived ER from 6:00P.M. to midnight were more satisfied than patients who arrived other time. 5. The length of stay at ER was significantly different according to the reason of hospital selection, the reason of ER selection, the visitant cause and hopital decisioner. Especially the length of stay was much longer in accident group and unconscious group.

• Advances in pediatric surgery
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• v.13 no.1
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• pp.52-60
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• 2007

Pediatric laparoscopic appendectomy is controversial particularly in complicated appendicitis. We evaluated the outcomes of laparoscopic appendectomy (LA) and open appendectomy (OA) in simple appendicitis and complicated appendicitis respectively. Since June 2004, initial LA has been our policy in all appendicitis including complicated ones. A total of 160 patients were included in this study, consisting of 80 OA (August 2001 - August 2003) and 80 LA (June 2004 - June 2006). We compared the operating time, the length of hospital stay, the length of antibiotics use, and the postoperative complications between LA and OA. In simple appendicitis (73), there were no differences between LA and OA. However in the 87 patients with complicated appendicitis, the operating time was longer in LA (64.8 min vs. 50.2 min) but the length of hospital stay was shorter in LA than OA (8.5 days vs. 9.6 days). There was one complication in simple appendicitis group and six in complicated appendicitis group (3 cases in LA, 3 cases in OA). There was no difference in the results of LA versus OA in simple appendicitis. Therefore for simple appendicitis, LA is recommended in consideration of the cosmetic effect (fewer scar). In complicated appendicitis, early discharge was an advantage and there were no differences in complications in LA despite a longer operative time. So we conclude LA can be considered as the first choice of treatment for all pediatric appendicitis including complicated appendicitis. To confirm our impressions, more well controlled randomized prospective studied need to be done.

• Korean Journal of Applied Biomechanics
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• v.16 no.2
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• pp.153-163
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• 2006

The purpose of this research was to analyze kinematic variables that appear during the instep shooting motion of female high school soccer players according to the angle of approach to find effective shooting motions. For this experiment, 5 female high school soccer players from the K city were participated in this study as the subject group, and as a through comparison and analysis of the resulting numbers of the variables, we came to the following conclusions. 1) Stride length and stride length/lower extremity length increased as the angle of approach increased. 2) As for C.O.G movement displacement, it was highest at an approach angle of $90^{\circ}$ during Right Foot Contact, at $135^{\circ}$ during Left Foot Contact, at $0^{\circ}$ during Rigth Toe Top, at $45^{\circ}$ during Impact, and at $0^{\circ}$ during Follow through. 3) The time required for each phase was longest at APP and shortest at BSP. The time required increased a little as the angle of approach increased, and the total time required also increased as the angle of approach increased. 4) The angle of the ankle joint was largest at an approach angle of $45^{\circ}$ for all events except Right Foot Contact. 5) The angle of the knee joint was largest at an approach angle of $135^{\circ}$ during Right Foot Contact, at $0^{\circ}$ during Left Foot Contact, at $45^{\circ}$ during Right Toe Top, at $135^{\circ}$ during Impact, and at $90^{\circ}$ during Follow through. 6) The angle of the hip joint was largest at an approach angle of $90^{\circ}$ during Right Foot Contact, at$0^{\circ}$ during Left Foot Contact, at $0^{\circ}$ during Right Toe Top, at $90^{\circ}$ during Impact, and at $0^{\circ}$ during Follow through.

• The Korean Journal of Pain
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• v.35 no.1
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• pp.97-105
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• 2022

Background: Percutaneous transforaminal endoscopic discectomy (PTED) has been widely used in the treatment of lumbar degenerative diseases. Epidural injection of steroids can reduce the incidence and duration of postoperative pain in a short period of time. Although steroids are widely believed to reduce the effect of surgical trauma, the observation indicators are not uniform, especially the long-term effects, so the problem remains controversial. Therefore, the purpose of this paper was to evaluate the efficacy of epidural steroids following PTED. Methods: We searched PubMed, Embase, and the Cochrane Database from 1980 to June 2021 to identify randomized and non-randomized controlled trials comparing epidural steroids and saline alone following PTED. The primary outcomes included postoperative pain at least 6 months as assessed using a visual analogue scale (VAS) and the Oswestry Disability Index (ODI). The secondary outcomes included length of hospital stay and the time of return to work. Results: A total of 451 patients were included in three randomized and two non-randomized controlled trials. The primary outcomes, including VAS and ODI scores, did not differ significantly between epidural steroids following PTED and saline alone. There were no significant intergroup differences in length of hospital stay. Epidural steroids were shown to be superior in terms of the time to return to work (P < 0.001). Conclusions: Intraoperative epidural steroids did not provide significant benefits, leg pain control, improvement in ODI scores, and length of stay in the hospital, but it can enable the patient to return to work faster.

• Korean Journal of Ichthyology
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• v.12 no.2
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• pp.137-145
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• 2000

The embryonic and larval development of Chelon lauvergnii (Eydoux & Souleyet) was surveyed by incubating artificially inseminated eggs with parent fishes obtained at Kang-wha island in the mid-western coastal area of Korea on June, 1997. The fertilized eggs were transparent, spherical in shape, measuring 0.95~1.08 mm in diameter, having a large oil globule, and their perivitelline space narrow, and began to hatch at 40 hrs. in water temperature $22{\pm}1^{\circ}C$. The newly hatched larvae were 2.35~2.68 mm in total length with 23 myomeres, anus opened, mouth closed, preanal length 58.7~61.6% of total length, oil globule located in posterior end of yolk sac. Melanophores, branch in shape, were distributed mainly along the ventro-lateral region of trunk part and a few on the anterior end of caudal part and surface of oil globule. The larvae measuring 3.08~3.36 mm in total length absorbed yolk material completely in 3 days after hatching, in which air bladder began to appear and mouth opened. In 8 days after hatching, the larva was measured 5.09 mm in total length, its posterior end of notochord began to flex upward and the caudal fin rays differentiated as 7, finfold of the second dorsal and anal fins appeared. In this time, melanophores, branch in shape, were concentrated in the anterior half region of the caudal part and a few also distributed on the top of head, snout region, ventral margin of lower jaw and isthmus region. In 12 days after hatching, the larva measuring 8.48 mm in total length completed all the fins (D. IV-9; P1. 16; P2. I, 5; A. II, 9) and reached to the juvenile stage. Melanophores, in this time, were distributed on the mid-lateral region of the caudal part in enlargment than before and a few also found in the dorso-lateral region of the trunk part, and in the cheek region.

• Journal of Welding and Joining
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• v.14 no.6
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• pp.48-57
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• 1996

The weldability of high purity aluminum-lithium binary alloys has been investigated using the Varestraint test. Autogenous GTAW (gas-tungsten-arc-welds) were run along specimens of different lithium concentration using three sets of welding parameters. Welding voltage was held constant at 10 volts. Welding current (70∼100 amps) and travel speed (23∼33 cm/min) were the parameter varied. Hot-tearing susceptibility varied with lithium content and exhibited a steep peak at 2.6 weight percent lithium. Depth of penetration increased with increasing heat input and lithium concentration. The susceptibility is influenced by the wettability of dendrites by the interdendritic eutectic liquid as well as the time available for back-Siting by eutectic liquid. The welding condition of welding current 70A and travel speed 23 cm/min was showed good resistance to cracking in aluminum-lithium alloys. Suggestions for improving weld cracking resistance are also provided.