• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.321-321
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• 2017

Water availability in rainfed lowlands (RFL) is strongly affected by climate change. In RFL, rice plants are exposed to soil moisture fluctuations (SMF) but rarely to simple progressive drought as widely believed. Typical RFL field is characterized by a about 5-cm thick high bulk density hardpan layer underneath the cultivated layer at about 20 cm depth that impedes deep root development. Root system has the ability to develop in response to changes in SMF, known as phenotypic plasticity. We hypothesized that genotypes that can adapt to RFL have root plasticity. The roots can sharply respond to re-wetting after drought period and thus penetrate the hardpan layer when the hardpan is wet and so becomes relatively soft, and thus access water under the hardpan. This study aimed to identify CSSLs derived from a cross between Sasanishiki and Habataki which adapted to such RFL conditions. We used 39 CSSLs together with the parent Sasanishiki, which were grown in hydroponics and pot under transient soil moisture stresses (drought and then rewatering), and compared with continuously well-watered (WW) (control) up to 14 days after sowing (DAS), and 20 DAS, respectively. Based on the results of hydroponics and pot experiments, we selected a few lines, which were grown in the soil-filled rootbox with artificial hardpan layer and without artificial hardpan. For the rootbox without artificial hardpan, plants were grown under WW and transient soil moisture stresses for 49 DAS. While the rootbox with artificial hardpan, the plants were grown under WW (control) and SMF (WW up to 21 DAS, 1st drought (22-36 DAS), rewatering (37-44 DAS), and followed by 2nd drought (45-58 DAS)). Among the 39 CSSLs, only CSSL439 (SL39) consistently showed significantly higher shoot dry weight (SDW) than Sasanishiki under transient soil moisture stress conditions as well as SMF conditions in all the experiments. Furthermore, under WW, SL39 consistently showed no significant differences from Sasanishiki in shoot and root growth in most of traits examined. SL39 showed significantly greater total root length (TRL) than Sasanishiki under transient soil moisture stress, which is considered as phenotypic plasticity in response to rewatering after drought period. Such plastic root development was the key trait that effectively contributed to root elongation and branching during the rewatering period and consequently enhanced the root to penetrate hardpan layer when the soil penetration resistance at hardpan layer reduced. In addition, using the rootbox with artificial hardpan layer ($1.7g\;cm^{-3}$, heavily compacted), SL39 showed greater root system development than Sasanishiki under SMF, which was expressed in its significantly higher TRL, total nodal RL, and total lateral RL at hardpan layer as well as at below the hardpan layer. These results prove that SL39 has plasticity that enables its root systems to penetrate hardpan layer in response to rewatering. Under SMF, such root plasticity contributed to its higher gs and Pn.

• Korean Journal of Microbiology
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• v.52 no.1
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• pp.1-9
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• 2016

A unique Schizosaccharomyces pombe $TrxR^+$ gene encoding thioredoxin reductase (TrxR) was found to be positively regulated by stress-inducing agents through the stress-responsive transcription factor Pap1. In the present study, the protective roles of S. pombe TrxR were evaluated using the TrxR-overexpressing recombinant plasmid pHSM10. In the presence of hydrogen peroxide ($H_2O_2$) and superoxide anion-generating menadione (MD), S. pombe TrxR increased cellular growth and the total glutathione (GSH) level, while it reduced levels of intracellular reactive oxygen species (ROS). The nitric oxide (NO) levels of the TrxR-overexpressing cells, in the presence of $H_2O_2$ and MD, were maintained to be similar to those of the corresponding non-treated cells. Although S. pombe TrxR was able to scavenge NO generated by sodium nitroprusside (SNP), it had no significant modulating effects on cellular growth, ROS levels, or the total GSH level of SNP-exposed yeast cells, compared with the differences in those of the two non-treated cell cultures. TrxR increased the cellular growth and total GSH level, which were diminished by nitrogen starvation. It also scavenged ROS and NO produced during nitrogen starvation. Taken together, the S. pombe TrxR protects against oxidative, nitrosative, and nutritional stresses.

• Journal of Ecology and Environment
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• v.30 no.2
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• pp.187-193
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• 2007

Higher plants can be categorized as C3, C4 or CAM according to their photosynthetic pathways, and some succulent plants are known to shift their patterns of photosynthesis from C3 to CAM in response to environmental stresses such as salt treatment or water deficiency. To investigate fundamental photosynthetic patterns and the induction of pattern shifts (C3, CAM, C3-CAM etc.) as a result of environmental stresses, we measured the water content, diurnal changes in pH, net $CO_2$ exchange, transpiration rate, total ionic contents, and osmolality of Kalancoe daigremontiana, Sedum kamschaticum and Sedum sarmentosum which belong to Crassulaceae known as representative CAM plant, after 10 days of drought treatment. S. kamschaticum and S. sarmentosum did not show a significant difference in diurnal pH variation in the treatment and control conditions. However, the pH of drought-treated Kalancoe was low at night and high in the daytime, with a pH value between 4 and 5. Typical CAM plants display a net $CO_2$ exchange that increases at night and decreases in the daytime. Kalancoe displayed the predicted pattern. However, S. kamschaticum and S. sarmentosum showed a photosynthetic pattern more typical of C3 plants, and did not show changes in photosynthetic pattern under drought stress. Kalancoe also showed a transpiration rate typical for CAM pho-tosynthesis, whereas the transpiration rates of S. kamschaticum and S. sarmentosum were in the typical range for C3 photosynthesis. Kalancoe had high total ionic contents during the night, which decreased somewhat during the daytime, whereas S. kamschaticum and S. sarmentosum displayed the opposite pattern. This result is similar to the diurnal patterns of changes in pH in the three plant species, which suggests a relationship between pH and ionic contents. S. sarmentosum showed lower osmolality under drought stress than in the control condition, whereas the osmolality of Kalancoe and S. kamschaticum did not differ between conditions. S. sarmentosum may have maintained internal water content by lowering its osmolality and raising its total ionic contents. In conclusion, Kalancoe displayed the characteristic responses of a typical CAM plant, whereas S. kamschaticum and S. sarmentosum displayed aspects of the C3 photosynthetic pattern under drought conditions. These results suggest that S. kamschaticum and S. sarmentosum (Crassulacea) in Korea overcome drought stress by increasing solute and ionic contents internally rather than changing their photosynthetic pattern from C3 to CAM under drought stress.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.03a
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• pp.193-200
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• 1999

An experimental study is described in which a 305-mm-diameter instrumented drilled shaft was installed in a moderately expansive clay soil during the dry season and monitored over a period of about 18 months. The purpose of the study was In investigate the effects of seasonal moisture changes in the soil on the shear stresses imposed on the sides of the drilled shaft and movements of the shaft head. The soil in the vicinity of the test shaft was instrumented to measure suction and ground surface movement and the relation between suction, total stress and shear strength of the soil at the test site was determined through laboratory triaxial compression testing. Daily rainfall and temperatures were also monitored at the test site, the National Geotechnical Experimentation Site at the University of Houston, where control on surface grading and vegetation existed. Over the course of the study induced unit side shear values of up to 54 kPa were measured in the test shaft. A simple computational model was developed that related observed suction changes to unit side shear induced by the expansion of the soil through the use of the laboratory suction-total stress-shear strength relation.

• Korean Journal of Computational Design and Engineering
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• v.13 no.1
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• pp.58-66
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• 2008

The artificial discs have recently used to preserve the motion of the treated segment in lumbar spine surgery. However, there have been lack of biomechanical information of the artificial discs to explain current clinical controversies such as long-term results of implant wear and excessive facet contact forces. In this study, we investigated the biomechanical effects of three artificial implants on the lumbar spinal segments by finite element analysis. The finite element model of intact lumbar spine(L1-S) was developed and the three implants were inserted in L4-L5 segment of the spine model. 5 Nm of flexion and extension moments were applied on the superior plate of L1 with 400 N of compressive load. Excessive motions and high facet contact forces at the surgical level were generated in the all three implanted models. In the flexion, the peak von-Mises stresses in the semi-constrained type implant was higher than those in the un-constrained type implant which would cause wear on the polyethylene core. The results of the study would provide a biomechanical guideline for selecting optimal surgical approach or evaluating the current design of the implants, or developing a new implant.

• Steel and Composite Structures
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• v.15 no.5
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• pp.481-505
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• 2013

This paper focuses on thermal post-buckling analysis of functionally graded beams with temperature dependent physical properties by using the total Lagrangian Timoshenko beam element approximation. Material properties of the beam change in the thickness direction according to a power-law function. The beam is clamped at both ends. In the case of beams with immovable ends, temperature rise causes compressible forces and therefore buckling and post-buckling phenomena occurs. It is known that post-buckling problems are geometrically nonlinear problems. Also, the material properties (Young's modulus, coefficient of thermal expansion, yield stress) are temperature dependent: That is the coefficients of the governing equations are not constant in this study. This situation suggests the physical nonlinearity of the problem. Hence, the considered problem is both geometrically and physically nonlinear. The considered highly non-linear problem is solved considering full geometric non-linearity by using incremental displacement-based finite element method in conjunction with Newton-Raphson iteration method. In this study, the differences between temperature dependent and independent physical properties are investigated for functionally graded beams in detail in post-buckling case. With the effects of material gradient property and thermal load, the relationships between deflections, critical buckling temperature and maximum stresses of the beams are illustrated in detail in post-buckling case.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.4
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• pp.45-51
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• 2020

In this study, a response spectrum analysis of an electric distributing board (EDB) was conducted to investigate seismic integrity in the design stage. For the seismic analysis, the required response spectra of a safe shutdown earthquake with 2% damping (RRS/SSE-2%) specified in GR-63-CORE Zone 4 was used as the ground spectral acceleration input. A finite element method modal analysis of the EDB was also performed to examine the occurrence of resonance within the frequency range of the earthquake response spectrum. Furthermore, static stress caused by deadweight was analyzed. The resultant total maximum stress of the EDB structure was calculated by adding the maximum stresses from both seismic and static loads using the square root of the sum of the squares (SRSS) method. Finally, the structural safety of the EDB was investigated by comparing the resultant total maximum stress with the allowable stress.

• Structural Engineering and Mechanics
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• v.44 no.1
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• pp.109-125
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• 2012

Post-buckling behavior of Timoshenko beams subjected to uniform temperature rising with temperature dependent physical properties are studied in this paper by using the total Lagrangian Timoshenko beam element approximation. The beam is clamped at both ends. In the case of beams with immovable ends, temperature rise causes compressible forces end therefore buckling and post-buckling phenomena occurs. It is known that post-buckling problems are geometrically nonlinear problems. Also, the material properties (Young's modulus, coefficient of thermal expansion, yield stress) are temperature dependent: That is the coefficients of the governing equations are not constant in this study. This situation suggests the physical nonlinearity of the problem. Hence, the considered problem is both geometrically and physically nonlinear. The considered highly non-linear problem is solved considering full geometric non-linearity by using incremental displacement-based finite element method in conjunction with Newton-Raphson iteration method. The beams considered in numerical examples are made of Austenitic Stainless Steel (316). The convergence studies are made. In this study, the difference between temperature dependent and independent physical properties are investigated in detail in post-buckling case. The relationships between deflections, thermal post-buckling configuration, critical buckling temperature, maximum stresses of the beams and temperature rising are illustrated in detail in post-buckling case.

• Computers and Concrete
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• v.5 no.3
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• pp.217-241
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• 2008

In this paper, a total strain-based hysteretic material model based on MCFT is proposed for non-linear finite element analysis of reinforced concrete structures. Although many concrete models have been proposed for simulating behavior of structures under cyclic loading conditions, accurate simulations remain challenging due to uncertainties in materials, pitfalls of crude assumptions of existing models, and limited understanding of failure mechanisms. The proposed model is equipped with a fully generalized hysteresis rule and is formulated for 2D plane stress non-linear finite element analysis. The proposed model has been formulated in a tangent stiffness-based finite element scheme so that it can be used for most general finite element analysis packages. Moreover, it eliminates the need to check that tensile stresses can be transmitted across a crack. The tension stiffening model is a function of the bar orientation and any orientation can be accommodated. The proposed model has been verified with a series of experimental results of 2D RC planar panels. This study also demonstrates how parameters of the proposed model associated with cyclic damage modeling influences the pinched cyclic shear behavior.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.1
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• pp.21-26
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• 2001

To develop transgenic orchardgrass resistant to reactive oxygen species produced from environmental stresses, a vector with the cytosolic glutathione reductase cDNA (BcGRl) from Chinese cabbage was constructed under the control of the cauliflower mosaic virus 35S promoter and was introduced into orchardgrass using Agrobacterium tumefaciens EHA101. Transgenic plants from hygromycin-selected calli of orchardgrass did not show any morphological difference from wild-type plants. The results of PCR amplification and genomic Southern blot analysis confirmed the integration of foreign gene into the chromosome of transgenic orchardgrass. Northern blot analysis with total RNA from leaves also confirmed the constitutive expression of BcGR1 in transgenic orchardgrass.