• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.396-397
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• 2005

The polyene antibiotics are a family of most promising antifungal polyketide compounds, typically produced by actinomycetes species. Using the polyene CYP-specific PCR screening with served actinomycetes genomic DNAs, Pseudonocardia autotrophica strain was identified to contain a unique polyene-specific CYP gene. The genomic DNA library screening using the polyene-specific CYP gene probe revealed the positive cosmid clone containing an approximately 34.5 kb DNA fragment revealed a total of seven complete and two incomplete open reading frame (ORFs), which are highly homologous but unique to previously-known polyene biosynthetic genes. These results suggest that the polyene-specific screening approach should be an efficient way of isolating potectially-valuable cryptic polyene biosynthetic gene cluster from various rare actinomycetes.

• Structural Engineering and Mechanics
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• v.13 no.6
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• pp.713-730
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• 2002

This paper presents a finite element approach for determining the natural frequencies for planar inclined arches of various shapes vibrating in three-dimensional space. The profile of inclined arches, represented by undeformed centriodal axis of cross-section, is defined by the equation of plane curves expressed in the rectangular coordinates which are : circular, parabolic, sine, elliptic, and catenary shapes. In free vibration state, the arch is slightly displaced from its undeformed position. The linear relationship between curvature-torsion and axial strain is expressed in terms of the displacements in three-dimensional space. The finite element discretization along the span length is used rather than the total are length. Numerical results for arches of various shapes are given and they are in good agreement with those reported in literature. The natural frequency parameters and mode shapes are reported as functions of two nondimensional parameters: the span to cord length ratio (e) and the rise to cord length ratio (f).

• Computers and Concrete
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• v.25 no.5
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• pp.461-470
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• 2020

This paper presents a numerical study on structural behavior of hammer head pier cap beams, extended on verges and reinforced with carbon fiber reinforced polymer (CFRP) plates. A 3-D finite element (FE) model along with a simplified analytical model are presented. Concrete damage plasticity (CDP) was adapted in the FE model and an analytical approach predicting the CFRP anchor strength was adapted in both FE and analytical model. Total five quarter-scaled pier cap beams with various CFRP reinforcing schemes were experimentally tested and analyzed with numerical approaches. Comparison between experimental results, FE results, analytical results and current ACI guideline predictions was presented. The FE results showed good agreement with experimental results in terms of failure mode, ultimate capacity, load-displacement response and strain distribution. In addition, the proposed strut-and-tie based analytical model provides the most accurate prediction of ultimate strength of extended cap beams among the three numerical approaches.

• Steel and Composite Structures
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• v.17 no.6
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• pp.867-890
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• 2014

This paper deals with the geometric nonlinear bending analysis of laminated composite stiffened plates subjected to uniform transverse loading. The eight-noded degenerated shell element and three-noded degenerated curved beam element with five degrees of freedom per node are adopted in the present analysis to model the plate and stiffeners respectively. The Green-Lagrange strain displacement relationship is adopted and the total Lagrangian approach is taken in the formulation. The convergence study of the present formulation is carried out first and the results are compared with the results published in the literature. The stiffener element is reformulated taking the torsional rigidity in an efficient manner. The effects of lamination angle, depth of stiffener and number of layers, on the bending response of the composite stiffened plates are considered and the results are discussed.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 1995.05a
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• pp.141-153
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• 1995

An incremental Total Lagrangian Formulation is implemented for the finite element analysis of laminated composite pressure vessel with consideration of the material and geometric nonlinearities. For large displacements/large rotations due to geometric nonlinearities, the incremental equations are derived using a quadratic approximation for the increment of the reference vectors in terms of the nodal rotation increments. This approach leads to a complete tangent stiffness matrix. For material nonlinearity, the analysis is performed by using the piecewise linear method, taking account of the nonlinear shear stress-strain relation. The results of numerical tests include the large deflection behavior of the selected composite shell problem. When compared with the previous analysis, tile results are in good agreement with them. As a practical example, filament wound pressure vessel is analyzed with consideration of the geometrically and materially nonlinearity. The numerical results agree fairly well with the existing experimental results.

• Structural Engineering and Mechanics
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• v.35 no.3
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• pp.349-364
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• 2010

The impact behaviour and the impact-induced damage in laminated composite cylindrical shell subjected to transverse impact by a foreign object are studied using three-dimensional non-linear transient dynamic finite element formulation. A layered version of 20 noded hexahedral element incorporating geometrical non-linearity is developed based on total Langragian approach. Non-linear system of equations resulting from non-linear strain displacement relation and non-linear contact loading are solved using Newton-Raphson incremental-iterative method. Some example problems of graphite/epoxy cylindrical shell panels are considered with variation of impactor and laminate parameters and influence of geometrical non-linear effect on the impact response and the resulting damage is investigated.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.11 no.1
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• pp.79-87
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• 1991

An efficient numerical procedure for material and geometric nonlinear analysis of reinforced concrete shells under monotonically increasing loads through their elastic, inelastic and ultimate load ranges is developed by using the finite element method. The 8-node Serendipity isoparametric element developed by the degeneration approach including the transverse shear deformation is used. A layered approach is used to represent the steel reinforcement and to discretize the concrete behavior through the thickness. The total Lagrangian formulation based upon the simplified Von Karman strain expressions is used to take into account the geometric nonlinearity of the structure. The material nonlinearities are taken into account by comprising the tension, compression, and shear models of cracked concrete and a model for reinforcement in the concrete; and also a so-called smeared crack model is incorporated. The steel reinforcement is assumed to be in a uniaxial stress state and is modelled as a smeared layer of equivalent thickness. This method will be verified a useful tool to account for geometric and material nonlinearities in detailed analysis of reinforced concrete concrete shells of general form through numerical examples of the sequential paper( ).

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.1A
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• pp.45-54
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• 2006

To obtain realistic stress-strain relation in concrete, it is necessary to improve the constitutive model for creep and shrinkage of concrete. This study is made up with predicting model of creep using rheological approach and mathematical development which is solution for phenomenon of concrete creep. Long-term deformation components are combined based on traditional 4-parameters model. Creep deformation is obtained adequately using 4-parameters determined by considering aging effect and microprestress among gels. And coefficient of effective viscosity is able to represent both basic creep and total creep included drying creep. This study attempt to establish mathematical model considering effects of aging, hydration, and variations of pore humidity. It can predict both basic creep and total creep. Values of result between prediction and experiment have greater than correlation factor 99%. Additionally experimental results report bad consentaneity with highway design specification adopting FIB MC 90. Rather than those are similar to FIB MC 90 rev.99.

• Smart Structures and Systems
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• v.21 no.4
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• pp.479-491
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• 2018

Shape memory alloys (SMA) exhibit superelasticity which refers to the capability of entirely recovering large deformation upon removal of applied forces and dissipating input energy during the cyclic loading reversals when the environment is above the austenite finish temperature. This property is increasingly favored by the earthquake engineering community, which is currently developing resilient structures with prompt recovery and affordable repair cost after earthquakes. Compared with the other SMAs, NiTi SMAs are widely deemed as the most promising candidate in earthquake engineering. This paper contributes to evaluate the seismic performance of properly designed concentrically braced frames (CBFs) equipped with NiTi SMA braces under earthquake ground motions corresponding to frequently-occurred, design-basis and maximum-considered earthquakes. An ad hoc seismic design approach that was previously developed for structures with idealized SMAs was introduced to size the building members, by explicitly considering the strain hardening characteristics of NiTi SMA particularly. The design procedure was conducted to compliant with a suite of ground motions associated with the hazard level of design-basis earthquake. A total of four six-story CBFs were designed by setting different ductility demands for SMA braces while designating with a same interstory drift target for the structural systems. The analytical results show that all the designed frames successfully met the prescribed seismic performance objectives, including targeted maximum interstory drift, uniform deformation demand over building height, eliminated residual deformation, controlled floor acceleration, and slight damage in the main frame. In addition, this study indicates that the strain hardening behavior does not necessarily impose undesirable impact on the global seismic performance of CBFs with SMA braces.

• Journal of Life Science
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• v.34 no.2
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• pp.79-85
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• 2024

SlyA is known as a transcriptional regulator that regulates the expression of hemolysin (HlyE) in E. coli, a member of the Enterobacteriaceae family such as Salmonella. However, Salmonella has the slyA gene but lacks the hlyE gene. Then, because we were curious about the role of SlyA in Salmonella, we constructed and explored a mutant strain with a deletion of the slyA gene. S. Typhimurium CK295 (ΔslyA) was constructed using an allelic exchange approach. In a comparative analysis between the wild-type and the CK295 strain, no significant differences were observed in growth characteristics, motility, total protein analyses, and secreted protein analyses. However, the CK295 strain exhibited slightly reduced biofilm formation compared to the wild-type. Interestingly, as a result of comparing the survival ability in macrophages, the mutant strain showed a 60% decrease in survival ability compared to the wild-type. To evaluate toxicity in mice, mortality was measured after oral administration to 6-week-old BALB/c mice. As a result, the LD₅₀ value of the CK295 (ΔslyA) was more than 100 times higher than that of wild-type S. Typhimurium 𝜒3339 in BALB/c. In conclusion, SlyA is presumed to regulate the expression of genes encoding virulence factors involved in the in vivo survival of Salmonella.