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Phytoplankton in the Waters of the Ieodo Ocean Research Station Determined by Microscopy, Flow Cytometry, HPLC Pigment Data and Remote Sensing (현미경, Flow Cytometer, HPLC 색소자료 및 원격탐사를 이용한 이어도 관측기지 주변수의 식물플랑크톤 연구)

  • Noh, Jae-Hoon;Yoo, Sin-Jae;Lee, Jung-Ah;Kim, Hyun-Chul;Lee, Jae-Hak
    • Ocean and Polar Research
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    • v.27 no.4
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    • pp.397-417
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    • 2005
  • Phytoplankton community structure and distribution pattern in the surface water around the Ieodo Ocean Research Station were investigated during seven cruises carried out from July, 2003 to October, 2004. Samples were analyzed using various tools including a microscope, flow cytometer, and HPLC. Satellite images were used to analyze spatio-temporal phytoplankton biomass distribution. SeaWiFS chlorophyll a (chl a) images showed that spring blooms occurred in April-May near the Ieodo Station, and these waters were under the influence of Changjiang Dilute Water during July-October. Also, during the July-October period, HPLC pigments data showed increasing zeaxanthin concentrations, a marker pigment of cyanobacteria whereas increasing concentrations of various other pigments such as fucoxanthin, peridinin, prasinoxanthia alloxanthin, 19'-hexanoyloxyfucoxanthin and chlorophyll b were noted during spring blooms. Such pigment marker data were consistent with picoplankton data analyzed by flow cytometer and nano-microplankton analyzed by microscope. The pigment-CHEMTAX method was used to drive the phytoplankton group apportioned chi a. Diatoms, chlorophytes, dinoflagellates, and cryptophytes comprised 25.8, 20.7, 15.9, and 14.1%, respectively, of the total chl a in May. Average cyanobacteria concentrations in July-October contributed 25.4% of the total concentration. This was the highest percent contribution and was followed by chlorophytes, diatoms, and prymnesiophytes. This study discusses results from various methods, similarities and differences in the results among those methods, and the application range of the results from different analytical methods. Also, the study reveals a detailed phytolpankton community structure in the waters around the Ieodo Station, and suggests future monitoring considerations in relation to cell morphology, ecology and diversity factors according to taxonomic groups.

Effects of a Slow-Release Fertilizer on Onion (Allium cepa L.) Cultivation in Southwestern Area (서남부 지역 양파 재배 시 완효성 비료 시용효과)

  • Lee, Eul Tai;Cho, Sang Kyun;Song, Yeon Sang;Jang, Young Suk;Choi, In Hu;Oh, Yong Bee
    • Horticultural Science & Technology
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    • v.19 no.4
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    • pp.476-482
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    • 2001
  • This experiment was conducted to evaluate the effect of slow-release fertilizer application on quality elevation of onion (Allium cepa L.) in southwestern sea. The slow-release fertilizers used were NIAST (National Institute of Agricultural Science and Technology) I, II, CDU (Crotonylidene Diurea), UF (Urea-Formaldehydes), and IBDU (Isobutylidene Diurea) 30, 50. Growth of the onion plant treated with slow-release fertilizer showed better than the control. However, a rate of infected plant to downy mildew was low as compared with control. Chlorophyll and total nitrogen contents were high in control, whereas firmness, soluble solid content, number and thickness of scaly leaves were high in slow-release fertilizer plot. In early maturing onion, total marketable bulb yield was higher in slow-release fertilizer plot than all other fertilizer treatments. Total marketable bulb yield in late maturing onion treated with slow-release fertilizer was slightly decreased. Decaying loss and the rate of sprouting during storage period decreased in slow-release fertilizer application. In summary, application of slow-release fertilizer increased marketable yield, storability after harvest and also reduced labor needs for top dressing.

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Notes on the Benthic Macrofauna During September 1997 Namdaecheon Estuary, Gangneung, Korea (강릉 남대천 하구역의 1997년 9월중 대형저서동물의 분포패턴)

  • 홍재상;서인수;윤건탁;황인서;김창수
    • Korean Journal of Environmental Biology
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    • v.22 no.2
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    • pp.341-350
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    • 2004
  • We examined estuarine macrobenthos in Namdaecheon estuary, Gangneung, Korea, September 22, 1997. A total of 56 species were found together with an abundance of 378 individuals $m^{-2}$/ and a biomass of 20.79 gWWt $m^{-2}$. The major dominant species were unidentified oligochaete followed by different species of polychaetes Hediste japonica, Rhynchospio glutaea, Poecilochaetus trilobatus, Scoloplos armiger, Spiophanes bombyx and a talitrid amphipod Platorchestia crassicornis. The study area was divided into two different groups of stations and species, based on the q -mode and r-mode clustering analysis. In case of q-mode, there are two groups: one is a marine station group and the other is estuarine group. The r-mode clustering analysis showed the two main communities as follows; 1) Marine species group occurred only at station 7, 8 and 9 and 2) Estuarine species group composed of the species present at station 1 to 6 and 10 to 15. In the number of species, the estuarine station group includes 13 species, whereas 43 species were presented in the marine station group. Total macrofaunal abundance and total biomass were higher in the marine station group than those in the estuarine group. The species diversity index was also high in the marine group (> 2), whereas it was less than ca. 1 in the estuarine group.

Spatial Characteristics of Meiobenthic Community of Kongfjorden Sediment in the Svalbard Island, the Arctic Sea (북극해 스발바드 군도 Kongsfjorden 퇴적물에 서식하는 중형저서동물 군집의 공간 특성)

  • Kim, Dong-Sung;Shin, Jae-Chul;Kang, Sung-Ho;Chung, Ho-Sung
    • Ocean and Polar Research
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    • v.27 no.3
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    • pp.299-309
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    • 2005
  • The community structure of meiobenthos was studied in the sediment of Kongfjorden, Spitsbergen of Svalbard Island in the Arctic Sea. Samples of meiobenthos were collected in August, 2003. Meiobenthic organisms were collected by SCUBA and van veen grab or acryl sub-corers 34mm in internal diameter, and were taken from upper sediment to a depth of 3cm at each station. A total of 26 meiofaunal groups were found in the sediment of Spitsbergen in Svalbard Island. Nematodes were the most dominant faunal group. Sarcomastigophorans, benthic harpacticoids, and nauplius larvae of crustaceans, were also important components of the meiobenthic community of Kongsfjorden. All of these low faunal groups were comprised of more than 90% of total meiobenthos at every station. The total density of meiobenthos at each station was highest at station MeG 6 $(3,583{\pm}1,137inds./10cm^2)$, and lowest at station $MeG9(28{\pm}1inds./10cm^2)$. Meiobenthos in general showed the highest density in the upper 1cm layer. This may be associated with food and oxygen supply to subsurface. Harpacticoids showed extreme preference at the surface and little presence in layers deeper than 2cm. These animals may be less resistant to oxygen deficiency, and nauplius also showed the same trend. However, in St. MeG 8 and 9, meiobenthos were dense at depths of more than 0-1cm, at especially at depths of 2-3m because of relatively easy penetration of oxygen. Based on the results of cluster analysis, three meiobenthos assemblages were distinguished: one was in the outer and two were in the inner fjord. Station SCU 5 was grouped with the meiobenthos assemblage located in the outer fjord. The outer ford community was characterised by : 1) a relatively low mean number of meiobenthos taxa, 2) a relatively high density of harpacticods and nauplius. One of the inner ford communities (a group of four nation: MeG 2, 3, 8, 9) was in the proximity of the glaciers. Specifically, it was characterised by : 1) a low mean number of meiobenthos taxa, 2) a low density. The other inner ford community was characterised by both a high density and great mean number of meiofaunal taxa.

A Study on Interconnectioned LAN of Token Ring Network (토큰링 네트워크가 상호 연결된 근거리 통신망에 관한 연구)

  • 이상훈;김경식;강준길
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.17 no.11
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    • pp.1206-1218
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    • 1992
  • Token ring systems which control to switch the data stream of networks by passing the token have been widely used to medium access controls in many ring or bus topology LANs. The system could be modeled for analysis as single-server-multi-queue system of the cyclic service method. These concepts could be expanded to multi-token ring systems interconnected with single ring consisting of bridges implemented simply to be stored and transmitted. In the proposal for the performance analysis of the interconnected token ring system, in has been assumed M/G/1 queueing model that frame arrivals are the Poisson process at each station queue and frame sizes are independently and identically distributed. And the average time delays were analyzed mathematically for arbitrary frame transferred from source station to destination area. The time delay of the frame transmission could be explained as the sum of the average time which the token passed from arbitrary position to source station, such as the waiting time in the source station transferring the previous arrival frames, and the propagation time from source station to interdestinated point. These delays were given as the sum of the duration from inner and outer bridge queues, the time delays from inner and outer bridge queues, and the time from outer bridge queue to destination station. These results were investigated by varing parameters effected to total time delays. In the results, those factors to be effected to dominant the total time delays were increased were in the cases of the high arrival rates and the high ration of destination of the other outerring. The system were shown the time delays increased exponentially in spite of the priority service policy. In order to decreasing the number of outerrings and increasing the number of nodes in backbone relatively, so the systems could be decreased the total delay in the interconnected token ring system.

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Status of Observation Data at Ieodo Ocean Research Station for Sea Level Study

  • Han, MyeongHee
    • Journal of the Korean earth science society
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    • v.41 no.4
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    • pp.323-343
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    • 2020
  • Observation data measured at Ieodo Ocean Research Station (IORS) have been utilized in oceanographic and atmospheric studies since 2003. Sea level data observed at the IORS have not been paid attention as compared with many other variables such as aerosol, radiation, turbulent flux, wind, wave, fog, temperature, and salinity. Total sea level rises at the IORS (5.6 mm yr-1) from both satellite and tide-gauge observations were higher than those in the northeast Asian marginal seas (5.4 mm yr-1) and the world (4.6 mm yr-1) from satellite observation from 2009 to 2018. The rates of thermosteric, halosteric, and steric sea level rises were 2.7-4.8, -0.7-2.6, 2.3-7.4 mm yr-1 from four different calculating methods using observations. The rising rate of the steric sea level was higher than that of the total sea level in the case with additional data quality control. Calculating the non-steric sea level was not found to yield meaningful results, despite the ability to calculate non-steric sea level by simply subtracting the steric sea level from total sea level. This uncertainty did not arise from the data analysis but from a lack of good data, even though tide, temperature, and salinity data were quality controlled two times by Korea Hydrographic and Oceanography Agency. The status of the IORS data suggests that the maintenance management of observation systems, equipment, and data quality control should be improved to facilitate data use from the IORS.

Chemical Components of Black Soybean Seeds Collected in Korea (수집 재래 검정콩의 화학적 성분)

  • Kim, Seok-Dong;Kim, Yong-Ho;Hong, Eun-Hi;Lee, Hong-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.1
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    • pp.1-7
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    • 1993
  • The 1,081 soybean genotypes including 972 black soybeans and 109 other colored soybeans were collected in 197 locations from January to April, 1991. Seed chemical components of soybean collections in relation to growth characters were evaluated to survey germplasms for black soybean breeding for seed quality. Seeds of 1,081 genotypes collected were analyzed for crude protein, crude lipid, and total sugar contents. The crude protein content was averaged to 39.8% and ranged from 34.1% to 48.0%. The average crude lipid and total sugar contents were 20.1% and 10.1%, and the ranges of those were 14.1% to 23.8% and 8.3% to 12.1%, respectively. Higher crude protein content was shown in early soybean maturity group, whereas higher total sugar content was in late group. Black soybean collections with green seed embryo tended to be higher in total sugar content than those with yellow one. Black soybean oil is generally composed of about 22% oleic, 55% linoleic, 7% linolenic acid, and 16% the others.

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Respiration Rates of Individual Bovine In Vivo-Produced Embryos Measured with a Novel, Scanning Electrochemical Microscopy (Scanning Electrochemical Microscopy를 이용한 한우 체내 수정란의 호흡률 조사)

  • Kim, Hyun;Bok, Nan-Hee;Kim, Sung-Woo;Do, Yoon-Jung;Kim, Min-Kyu;Cho, Sang-Rae;Seong, Hwan-Hoo;Kim, Dong Hun;Ko, Yeoung-Gyu
    • Journal of Embryo Transfer
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    • v.29 no.1
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    • pp.91-99
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    • 2014
  • Oxygen consumption is a useful parameter for evaluating mammalian embryo quality, since individual bovine embryos was noninvasively quantified by scanning electrochemical microscopy (SECM). Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we measured to investigate the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of pluripotent gene and anti-oxidant enzyme was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). Oxygen consumption of blastocyst was measured using a SECM and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts ($10.2{\times}10^{15}/mols^{-1}$ versus $6.4{\times}10^{15}/mols^{-1}$, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7 and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0 and over $12.0{\sim}10^{15}/mols^{-1}$, respectively. Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0 and over $12.0{\times}10^{15}/mols^{-1}$, respectively. GPX1 and SOD1 were significantly increased in over -10.0 group than below 10.0 groups but in catalase gene, there was no significant difference. On the other hand, In OCT-4 and Sox2, pluripotent gene, there was a significant difference (p<0.05) between the below-10.0 ($0.98{\pm}0.1$) and over 10.0 ($1.79{\pm}0.2$). In conclusion, these results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.

Studies on Bovine Mastitis in Seongwhan Area 1. Epidemiological Investigation (성환지역(成歡地域)의 유우유방염(乳牛乳房炎)에 관한 연구(硏究) 1. 유방염(乳房炎)의 발생실태(發生實態)와 그 원인균(原因菌) 조사(調査))

  • Seok, Ho Bong;Lee, Kwang Won;Oh, Seong Yong
    • Korean Journal of Veterinary Research
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    • v.21 no.2
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    • pp.161-165
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    • 1981
  • A total of 210 dairy cows of Seongwhan area were epidemiologically investigated for mastitis by Milk Quality Test(MQT) method. Milk samples from cows infected with mastitis were also bacteriologically studied. Seventy four (42.5%) of 174 dairy cows were positive for mastitis and fifty nine (79.7%) among them were infected with subclinical mastitis and fifteen (20.3%) were clinical mastitis. High infective rate were shown on August (48.7%) and July (45.7%) and it was lowered on December (35.8%). The incidence rate of subclinical mastitis for herd shown tendency of increase at the barn in which located on lower place (30.7~43.9%), whereas, the barn in which was on higher place and with bucket type milking system shown tendency of decrease causative organisms. A total of 230 strains of etiological agents were isolated from 578 infected duarters which shown MQT score (+) or more. The relevant causative organisms isolated in this study were Staphylococcus aureus (18.3%), Staphylococcus epidermidis (16.9%), fungi (16.5%), Pseudomonas aeruginosa (15.2%), Escherichia coli (7.8%) and Serratia marcescens (2.27), respectively.

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