• Title/Summary/Keyword: total plate count

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Effects of freezing storage temperature on the storage stability of beef (냉동 저장 온도가 쇠고기의 저장성에 미치는 영향)

  • Park, Jeong-Ah;Joo, So Young;Hwang, Hyun Jung;Na, Ye Seul;Kim, Seo Jin;Choi, Jeong In;Ha, Joo Young;Cho, Mi Sook
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.301-305
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    • 2016
  • This study was conducted to investigate changes in pH, volatile basic nitrogen (VBN), thiobarbituric acid (TBA), $a^*$ (redness), shearing force, and microbial content (total plate count; TPC) in beef during freezing storage at -1, -5, and $-20^{\circ}C$ for 21 days. TPC as an effective quality indicator was used to identify the decay point by regression analysis. The pH, TBA values, and TPC significantly increased with storage at all tested temperatures (p<0.05). VBN significantly increased and shearing force significantly decreased with storage at -1 and $-5^{\circ}C$ (p<0.05). TPC showed significant correlation with temperature at $-1^{\circ}C\;(R^2=0.891)$, $-5^{\circ}C\;(R^2=0.856)$, and $-20^{\circ}C\;(R^2=0.444)$. The decay points at -1, -5, and $-20^{\circ}C$, were 27, 52, and 84 days, respectively. The results suggest that a freezing temperature of $-5^{\circ}C$ is effective for short-term storage of beef.

Microbiological Quality of the Shellfish-growing Waters and Mussels in Changseon, Namhae, Korea (남해 창선해역의 해수 및 진주담치의 위생학적 안전성 평가)

  • Yoo, Hyun-Duk;Ha, Kwang-Soo;Shim, Kil-Bo;Kang, Jin Yeong;Lee, Tae-Seek;Kim, Ji-Hoe
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.4
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    • pp.298-306
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    • 2010
  • A sanitary survey was conducted to evaluate the water quality and mussel (Mytilus edulis) conditions of two administrative shellfish growing waters: those designated as shellfish growing water for export, and adjacent waters on the east coast of Changseon Island, Namhae, Korea. In all, 1,656 seawater and 166 mussel samples were collected at 46 stations for seawater and five stations for the shellfish from January 2007 to December 2009. Both seawater and mussels were examined for total coliforms and fecal coliforms. The standard plate count and most probable number of Escherichia coli were also determined for the shellfish samples. The range of the geometric means and the estimated 90th percentiles of fecal coliform for seawater samples at each station were <1.8-4.1 MPN/100 mL and <1.8-22.3 MPN/100 mL, respectively. The sanitary conditions for both shellfish growing areas, the designated shellfish growing area and adjacent area in Changseon, met the 'Approved area' criteria of the United States National Shellfish Sanitation Program. The range of E. coli detected in mussels in these areas was <20-500 MPN/100 g; and only 3 of 166 samples exceeded 230 MPN/100 g, the European standard for the consumption of raw bivalves. Therefore, the mussel farms in the Changseon area are classified as 'Class A' according to criteria set in European Community regulations. However, the levels of E. coli in mussels in parts of the designated shellfish growing area and adjacent area exceeded the bacterial limits after rainfall exceeding 45 mm. Further studies are needed to fully define the conditions leading to a temporary closure to harvest after a rainfall event.

Effects of Feeding Fermented Colostrum Feed on the Growth to Piglets (발효초유사료 급여가 자돈의 성장에 미치는 영향)

  • Na, Seuk-Han;Choi, Seong-Hyun;Renchinthand, Gereltuya;Bae, Hyoung-Churl;Nam, Myoung-Soo
    • Food Science of Animal Resources
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    • v.28 no.3
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    • pp.355-362
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    • 2008
  • This study was carried out to assess the fermentation properties of lactic acid bacteria (LAB) isolated from bovine colostrum and effects of feeding fermented colostrum feed on the growth to piglet. A total of 427 colonies were isolated from bovine colostrum on the BCP plate count agar. These LAB isolated were subcultured in 10% reconstituted skim milk, and seven strain thereof were selected for their highest acid productions, and two strain thereof were finally selected for their excellent sugar utilization. These strains were identified as Streptococcus thermophilus and Streptococcus macedonicus based on l6S rDNA sequencing data, named S. thermophilus CNB-11 and S. macedonicus CNB-11 respectively. For fermentation profiles, sugar utilization, acid production and viable cell counts were excellent in S. thermophilus CNB-11 as compared with S. macedoniclts CNB-11 after 48 hour. The effect of feeding fermented colostrum feed 0.5% using S. thermophilus CNB-11 was investigated for growth rate, analysis of blood and incidence of diarrhea. 24 heads of piglets were divided into two groups: the experimental and the control of 12 animals each. The average growth rate in the pigs fed fermented colostrum feed was higher 16.73% compared with control diet (p<0.05). There were no differences in the concentrations of blood glucose, cholesterol, albumin and globulin in pigs fed fermented colostrum feed as compared with control piglets. Incidence of diarrhea was no in pigs fed fermented colostrum feed as compared with control piglets.

Effects of Feeding TMR Feed with Dietary Citrus Byproducts on Chemical Properties of Raw Milk from Holstein Cow's Raw Milk (원유의 이화학적 특성에 미치는 감귤 부산물 TMR 사료 급여의 영향)

  • Moon, Yoon-Hee;Yang, Seung-Ju;Jung, In-Chul
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.5
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    • pp.723-728
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    • 2009
  • In this study, the effects of feeding providing Holstein cows with with TMR feed including amended with undried citrus byproducts on the properties of their raw milk were investigated. Two samples were used for the experiment: T0 (raw milk produced by dairy cows fed with TMR feed not including citrus byproducts) and T1 (raw milk produced by cows fed with TMR feed including citrus byproducts). The All experiments were conducted with Holstein cows at a dairy farm in the on Jeju island, and were repeated three times, in each, after of which raw milk obtained from 7 cows of each samplein each group was analyzed[ED highlight - please ensure this is correct]. The daily milk yield and somatic cell numbers of T0 and T1 were 24.16 kg and 25.97 kg, and 660,000 thousands and 445,000 thousands, respectively, which means that feeding citrus byproducts to cows increases daily milk yield and reduces somatic cell numbers. There was were no significant differences between T0 and T1 in terms of the raw milk's total plate count, specific gravity, titration acidity, and or the amount of milk protein, lactose, nonfat solids, free amino acids and volatile compounds, while raw milk in T1 showed significantly lower levels of milk fat in raw milk. The antioxidant activity of raw milk was improved by citrus byproducts TMR feed containing citrus byproducts.

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Effect of Microwave Treatment on the Physicochemical and Microbiological Characteristics of Beef Loin during Storage at 4℃ (마이크로파 처리가 4℃ 저장 중 우육의 이화학적 및 미생물학적 특성에 미치는 영향)

  • Kang, Ho Jin;Lee, Hyun-Yu;Park, Jong-Dae;Kum, Jun-Seok
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.161-166
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    • 2013
  • This study was carried out to investigate the physicochemical and microbiological characteristics of beef loin following microwave treatment at $4^{\circ}C$. Two types of microwave treatment were applied. i.e., continued microwave treatment (CW) and holding microwave treatment (HW). The L value increased, while a and b values were not significantly different among the samples as the storage time and microwave dose increased. The initial pH and after 3 days ranged from 5.51-5.74 and 6.32-6.51, respectively (p<0.05). The thiobarbituric acid value of all beef loins increased with increasing storage period and decreased with increasing microwave dose. The volatile basic nitrogen (VBN) content of the control was higher than that of the other samples and the VBN content decreased with increasing microwave dose. The total plate count of beef loins decreased with increasing microwave dose. This result indicated that T2 (100 W, HW) is most effective treatment with regard to the safety of beef loins without decreasing the physicochemical and microbiological characteristics.

Quality and Shelf-life of Chilled Smoked Pork Belly Depending on Packaging Methods (포장방법에 따른 냉장 훈제삼겹살의 품질과 저장성)

  • Lim, Ji-Hoon;Cheong, Sung-Hee;Lee, Sung-Ki;Lee, Keun-Taik
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.16 no.2_3
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    • pp.43-51
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    • 2010
  • The changes in quality and shelf life of smoked pork belly according to packaging methods were investigated during storage at $5{\pm}1^{\circ}C$ up to 12 days. Three packaging treatments including air-containing packaging (AC), vacuum packaging (VP), and packaging incorporated with oxygen scavenger (OS) were applied in this experiment. In all treatments, the initial total aerobic plate count (TPC) was 2.4 log cfu/g which was increased with storage period. The rapidest increase of TPC was observed in the AC samples, followed by OS and VP. The VP samples showed inhibiting effects on the growth of Pseudomonas spp. and coliforms over the storage. The TBA values were increased in the order of AC-OS-VP. The VBN values of the VP and OS samples tended to be increased slower than the AC samples during the storage. According to the sensory evaluations, the point of losing marketability in the raw AC, OS, and VP samples was determined to be the day 8, 10, and 12, respectively. However, the evaluations for the cooked samples showed the AC and OS samples preserved the marketing value until the day 10 and 12, while the VP samples until the day 12 except in the parameter of texture. In conclusion, it was found that the most appropriate packaging method for preserving the quality and extending the shelf life of chilled smoked pork belly is vacuum packaging.

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The Effects of Timber Harvesting on Soil Chemical Ingredients and Stream Water Quality (성숙임목수확벌채가 토양의 화학성분과 계류수질에 미치는 영향)

  • 박재현;우보명;김우룡;안현철;김재수
    • The Korean Journal of Ecology
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    • v.23 no.1
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    • pp.9-15
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    • 2000
  • The effects of clearcutting on soil chemical ingredients and stream water quality have been investigated at a natural deciduous forest catchment within the Seoul National University Research Forest in Mt. Paekun, Chunnam province during the periods of 1993 to 1998. Soil chemical ingredients and stream water qualities were monitored at a 13 ha clearcutting site and a non-treatment site nearby. During the first and second years after harvesting, the levels of total-N, and exchangeable ions (K/sup +/, Na/sup +/, Ca/sup 2+/, Mg/sup 2+/) decreased compared to the values of before harvesting. During the fifth years after harvesting, these levels were significantly higher than those during the first and second years after harvesting. But the chemical characteristics of soil were not changed at all. pH of water in the harvesting area was 6.5 in stream water. Among the nutrients, Cd, Pb, Cu, and phosphate were not found, and the level of BOD reached at the level of the domestic use suitable for drinking. Turbidity, odor, taste, NH/sub 4//sup +/ -N, NO/sub 3//sup -/-N, standard plate count, and coliform were also low enough to be used as the domestic use for drinking by the near villagers. During the first and second years after harvesting, BOD increased to about 1 ppm. For that reason, the harvesting planning should be built in the harvesting area in consideration of the control of water quality in the stream.

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Effects of freezing-storage temperature on the shelf life of mackerel fish (냉동 저장 온도가 고등어의 저장성에 미치는 영향)

  • Joo, So Young;Park, Jeong Ah;Hwang, Hyun Jung;Kim, Seo Jin;Choi, Jeong In;Ha, Joo Young;Cho, Mi Sook
    • Korean Journal of Food Science and Technology
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    • v.48 no.6
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    • pp.536-541
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    • 2016
  • The purpose of this study was to evaluate changes in the quality characteristics of mackerel fish fillet under freezing storage conditions. The changes in product quality were determined by measuring pH, volatile basic nitrogen (VBN); thiobarbituric acid (TBA), lightness ($L^*$), redness ($a^*$), yellowness ($b^*$) values, shearing force, and microbiological evaluation (Total Plate Count, TPC) during 21 days of storage at -20, -5, and $-1^{\circ}C$, and their shelf-lives were established. TPC as an effective quality indicator was used to estimate the shelf-life via linear regression analysis. The TPC increased steadily with increasing storage periods at every temperature (p<0.05) and showed a significant relationship with temperature at $-1^{\circ}C$ (r=0.949), $-5^{\circ}C$ (r=0.919), and $-20^{\circ}C$ (r=0.905). Therefore, based on TPC, the shelf life of the mackerel fillet was noted to be 32 days when stored at $-1^{\circ}C$, more than 51 days when stored at $-5^{\circ}C$, and about 2 months when stored at $-20^{\circ}C$.

Effect of $CO_{2}$ Gas Packaging on the Shelf-life of Refrigerated Pork Cuts (탄산가스 포장에 의한 신선돈육의 저장성향상에 관한 연구)

  • Kim, Yong-Su;Kim, Yun-Ji;Yoo, Ick-Jong
    • Korean Journal of Food Science and Technology
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    • v.26 no.3
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    • pp.246-249
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    • 1994
  • Shelf-life of pork packaged under $CO_{2}$ was observed and the optimal volume of $CO_{2}$ gas was determined. High gas barrier film, EVOH($CO_{2}$ permiability; 1 $cc/cm^{2}{\cdot}24\;h{\cdot}atm$) was used as packaging material and the volume ratios of sample to $CO_{2}$ of 1 : 1, 1 : 3 and 1 : 5 were tried using the control of vacuum packaged pork. To ev.3luate quality of pork cuts, total plate counts, TBA, color and sensory score were monitored during the storage at $4{\pm}1^{\circ}C$ for 31 days. TPC of vacuum packaged pork increased up to $4.6{\times}10^{6}\;CFU/cm^{2}$ in 24 days while that of $CO_{2}$ gas packaged pork $4.5{\times}10^{5}\;CFU/cm^{2}$ at the same storage period, and it took 7 days more for gas packaged pork to reach the same TPC count. There was no significant difference in TBA value between vacuum packaged and gas packaged pork. In the color L and a values were not affected by treatment but vacuum packaged pork cuts showed higher b value than gas packaged pork. According to sensory evaluation, gas packaged pork showed less off flavor, better flavor and freshness than vacuum packaged one (P<0.05).

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Changes in Quality Characteristics of Vacuum-Shrink Packaged Bierschinken after Stuffing in Fibrous Casing during Chilled Storage (화이브러스 케이싱에 충전 후 진공 수축 포장한 비어슁켄의 냉장 중 품질 특성 변화)

  • Lee Jung-Pyo;Lee Keun-Taik
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.121-126
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    • 2005
  • The changes in quality characteristics and shelf-life of vacuum-shrink packaged Bierschinken after stuffing in fibrous casing and stored at 5 and $10^{\circ}C$ were investigated The total aerobic plate count (APC) of Bierschinken was initially <2.00 $log_{10}CFU/cm^2$, however increased gradually over storage period The APCs of Bierschinken maintained at the level of 4.86 and 5.13 $log_{10}CFU/cm^2$ after 35 days at 5 and $10^{\circ}C$, respectively. pH tended to decrease with the extension of storage period The values of TBA and VBN tended to increase with storage time, of which trend was pronounced at $10^{\circ}C$ rather than at $5^{\circ}C$. According to the sensory evaluation, Bierschinken had the marketing value till 28 days irrespective of storage temperature. The shelf-life of Bierschinken tested might be preferentially restricted by the occurrence of off-odor and off-flavor at the end of storage period.