• 한국작물학회지
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• 제49권2호
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• pp.82-88
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• 2004

The objective of this study was to determine the role of storing years with the variation of total phenol and individual phenolic compounds in soybean (Glycine max L.) seeds. The total phenol content varied from 0.36 to 0.42% over four years, with the highest value (0.42%) found at storage for two and three years. Among the nine soybean varieties examined, Daweonkong had the highest total average phenol content (0.58%). The total content of 11 phenolics varied from 730.0 to 1812.8 $\mu\textrm{g}\;\textrm{g}^{-1}$ over storage for four years, and the highest concentration (1812.8 $\mu\textrm{g}\;\textrm{g}^{-1}$) was found at storage for two years. Myeongjunamulkong (1465.4 $\mu\textrm{g}\;\textrm{g}^{-1}$) had the highest mean content among the nine soybean varieties. The total content of 11 phenolic compounds measured in this study occupied from 20.96 to 47.73% of the total phenol contents. The highest total phenol contents were in seeds with black coats (5279.4 $\mu\textrm{g}\;\textrm{g}^{-1}$), while the highest concentration of individual phenolic compounds were in seeds with green coats (1419.5 $\mu\textrm{g}\;\textrm{g}^{-1}$). Our study suggests that it may be feasible to improve soybean varieties with high functional substances such as phenolic compounds.

• 한국약용작물학회지
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• 제28권5호
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• pp.331-338
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• 2020

Background: The aim of this study was to analyze the total phenol and total flavonoid contents and antioxidant activity of steam-treated leaves and rhizomes of Polygonatum sibiricum Redoute. In addition, we aimed to confirm their potential use as cosmetic materials by investigating their anti-aging and skin-whitening activity. Methods and Results: The leaves and rhizomes of P. sibiricum were treated with steam at different temperatures for different durations, and the antioxidant activity (DPPH and ABTS radical scavenging activity) and total phenol and total flavonoid contents of each sample were tested. The steam temperature and treatment duration siginificantly affected the antioxidant activity and, total phenol and total flavonoid content of the leaves and rhizome of P. sibiricum. Treating the P. sibiricum samples with steam at 120℃ for 12 h, yielded higher total phenol and total flavonoid contents. Comparatively, the samples treated with steam at 120℃ for 12 to 24 h showed significantly higher antioxidant activity. Further, the steamed samples of P. sibiricum demonstrated collagenase and tyrosinase inhibition activity, which indicated their anti-aging and skin-whitening properties. The samples steamed at 120℃ for 12 h, exhibited higher collagenase and tyrosinase inhibition activity. Conclusions: Leaves and rhizomes of P. sibiricum steamed at 120℃ for 12 h, showed highest antioxidant activity and, total phenol and total flavonoid contents than all other samples. Our results indicate the potential of using P. sibiricum as a cosmetic material by confirming its excellent anti-aging and whitening activity.

• 한국환경과학회지
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• 제25권5호
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• pp.695-703
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• 2016

The purpose of this study was to measure the bioactivity and antioxidant activity of Orostachys japonicus A. Berger. Orostachys japonicus A. Berger have been known to contain functional materials such as kaempferol, hydroquinone, methyl gallate, quercetin, gallic acid etc. To identify the main functional materials of Orostachys japonicus A. Berger, the contents of flavonoid and phenol were measured. We extracted Orostachys japonicus A. Berger powder from four solvents such as chloroform:methanol (CM, 2:1, v/v), distilled water (DW), 70% methanol, 70% ethanol. After that, this study determined tannin, total phenol, flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, ferric reducing antioxidant power and reducing power of Orostachys japonicus A. Berger extracts and as results of comparing each extract. respectively. From the above results shows that antioxidant activity and bioactivity of Orostachys japonicus A. Berger extracts was higher in the order of 70% ethanol, 70% methanol, DW and CM (p<0.05). The results showed that antioxidant activity of Orostachys japonicus A. Berger extracts supposed to affect by the total phenol and flavonoid contents.

• Preventive Nutrition and Food Science
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• 제15권3호
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• pp.244-247
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• 2010

This study was performed to evaluate the effect of drying methods on the polyphenol contents and antioxidant activities of Hizikia fusiformis. The seaweed was dried using four different conditions: vacuum drying (VD) at $20^{\circ}C$ and $30^{\circ}C$ and hot-air drying (HD) at $40^{\circ}C$ and $60^{\circ}C$. After drying, the total phenol content, DPPH and nitrite scavenging activities of the water extracts were determined. The total phenol contents were significantly (p<0.05) higher in the vacuum dried samples than the hot-air dried samples. DPPH radical was scavenged 82.67% and 83.45% by VD-20 and VD-30, and 70.44% and 71.23% by HD-40 and HD-60, respectively. At pH 1.2, Hizikia fusiformis scavenged 87.43%, 88.45%, 72.14% and 73.45% of nitrite by the treatment VD-20, VD-30, HD-40 and HD-60, respectively. Interestingly, the nitrite scavenging activity was much more reduced at pH 3.0 than at pH 1.2. Through these experiments, we were able to show that the drying conditions affect the total phenol contents and antioxidant capacities of Hizikia fusiformis.

• 한국연초학회지
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• 제23권2호
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• pp.162-167
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• 2001

This study was carried out to quantitatively determine phenol compounds in smoke total particulate matter(TPM) to evaluate the qualititive characteristics of flue-cured tobacco varieties(NC82, KFl14 and KFl18). After collecting The TPM by using smoking machine, the concentration of phenol compounds were analyzed by gas chromatography as their trimethylsilyl derivative on a fused capillary column bonded with a 5％ PE ME siloxane stationary phase. In all the flue-cured tobacco varieties, the major phenol components were monohydroxy compounds. The order of the highest concentration of total phenol compounds in TPM was NC82, KF114 and KFl18 but the contents of dihydroxy compound in the KFl18 was higher than those of NC82 and KF114.

• 한국식품영양학회지
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• 제24권4호
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• pp.702-707
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• 2011

The purpose of this study was to analyze the bioactive compounds of soybean snacks and to carry out a sensory evaluation of those variations which had various seasoning mixing ratio. Five soybean snacks were developed for these experiments. Proximate composition, the contents of total phenol compounds and isoflavone, and sensory characteristics of the soybean snacks were measured. There were significant differences in amounts of isoflavones and total phenolic compounds of soybean snacks($p$ <0.05). The seasoned soybean snacks had a higher level of daidzein and genistein contents than did control group. The total phenol compound contents of the control group was 210.3 mg gallic acid equivalents/100 g, whereas those for individual soybean snacks(A~E) were 152.3, 160.2, 162.4, 158.4 and 164.6 mg gallic acid equivalents/100 g, respectively. In sensory evaluation, there were significant differences($p$ <0.05) in taste, texture, and overall acceptability of the soybean snacks.

• 동아시아식생활학회지
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• 제24권3호
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• pp.392-399
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• 2014

산사에 함유되어 있는 생리활성 물질의 특성을 알아보고 70% methanol, 70% ethanol, chloroform:methanol(CM, 2:1, v/v), n-butanol 및 ethyl acetate(EA)의 5가지 용매를 사용하여 추출한 추출물의 용매별 항산화 활성 측정을 통하여 산사의 항산화 활성 및 천연 기능성 식품 재료로서의 가치를 검토하고자 본 실험을 수행하였다. 산사 과육의 total phenol 함량은 70% methanol, 70% ethanol, CM, EA, n-butanol 추출물 순으로 높게 나타났으며, 특히 70% methanol 및 70% ethanol 추출물은 각각 71.46 mg/g, 70.41 mg/g의 높은 함량을 보였다. Total flavonoid 함량은 CM(1.63 mg/g), n-butanol(1.59 mg/g), 70% methanol(1.26 mg/g), 70% ethanol(1.26 mg/g) 및 EA(1.12 mg/g) 순으로 높게 측정되었다. $NO_2$ radical 소거 활성은 total phenol 함량과 동일한 경향으로 나타났으며, ${\beta}$-carotene에서의 항산화 활성은 70% methanol, 70% ethanol, n-butanol, CM, EA 추출물, superoxide dismutase(SOD) 유사 활성은 70% methanol, 70% ethanol, CM, n-butanol, EA 추출물 순으로 높은 것으로 나타났다. 대부분 70% methanol 및 70% ehtnaol 추출물에서 활성이 높게 측정되었으며, 이는 산사 속에 함유되어 있는 total phenol 및 flavonoid와 같은 항산화 성분 때문인 것으로 추정되며, 특히 total phenol에 의한 것으로 생각된다. 따라서 산사는 항산화제 및 기능성 식품 소재로 활용 가능성이 높을 것으로 사료된다.

• 한국식품저장유통학회지
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• 제11권4호
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• pp.455-460
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• 2004

본 연구는 추출용매 및 주정에 의한 탈지 및 비탈지 포도종자 추출물의 수율, 항산화활성 및 총페놀함량을 분석하였으며, 각종 pH에서 처리된 추출물의 저장시 항산화력 변화를 살펴보았다. 추출수율은 탈지 및 비탈지 처리한 포도종자 분말 모두 $70\%$ 에탄올로 $70^{\circ}C$에서 6시간 추출하였을 때 각각 $8.9\%,\;9.16\%$로 다른 유기용매에 비하여 가장 높은 것으로 나타났으며, methanol, acetone-acetic acid 및 acetone 순으로 나타났으며 $70\%$ ethanol 탈지추출물의 항산화활성이 $41.52\;{\mu}g$으로 나타나 다른 용매의 추출물보다 훨씬 높았다. 포도종자 추출물은 탈지 및 비탈지추출물 모두 주정농도는 $90\%$, 추출온도는 $70^{\circ}C$, 추출시간은 6시간에서 가장 항산화활성이 좋았으며 $70^{\circ}C$에서 추출시간이 길어질수록 열에 의한 변성으로 인하여 항산화활성이 오히려 감소하였다. 이러한 결과는 같은 조건하에서 각 추출물의 total phenol 함량을 측정한 결과와 유사한 것으로 나타났다. 탈지 및 비탈지 추출물의 경우, ethylacetate 분획층이 $RC_{50}$값이 14.78, $12.35{\mu}g/mL$로 항산화활성이 가장 컸으며, buthanol, water, chloroform, hexane 분획층로 나타났다. 한편, 탈지 및 비탈지 포도종자 추출물을 pH 2, 7, 10의 표준 buffer 용액에 녹인 후 $50^{\circ}C$에서 1달간 저장하여 항산화력을 측정한 결과, 산성 조건보다는 알칼리성조건에서 더욱 항산화활성이 감소하였으며 건조상태로 저장시 고온에 매우 안정한 것으로 나타났다.

• Preventive Nutrition and Food Science
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• 제11권1호
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• pp.84-87
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• 2006

Antioxidant activity of Akebia quinata Decne was evaluated. Water extract (0.5 g/50 mL) of flowers and leaves of A. quinata were prepared and total phenol contents and radical scavenging activity of the extracts was determined for antioxidant activity. The total phenol contents of extracts from A. quinata flowers (FAQ) and leaves (LAQ) were $30.05{\mu}M\;and\;20.23{\mu}M$, while the radical scavenging activity of FAQ and LAQ were 60.51 % and 52.97%, respectively. In addition, the effect of FAQ and LAQ extract on DNA damage induced by $H_2O_2$ in human lymphocytes was evaluated by comet assay. The FAQ and LAQ showed strong inhibitory effect against DNA damage induced by $200{\mu}M$ of $H_2O_2$. These results suggest that water extracts of A. quinata Decne flowers and leaves showed significant (p<0.05) antioxidant activity and protective effect against oxidative DNA damage.

• 한국식품영양학회지
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• 제31권5호
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• pp.689-695
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• 2018

This study was carried out to select apple cultivars suited for the fresh cutting or minimal processing as investigating correlation between total phenol and ascorbic acid as browning factors and the enzymatic browning degree. In soluble solid-acid ratio, 'Gamhong' and 'Arisoo' were highest value of 71.69 and 71.52 compared with other cultivars. And the change of lightness (${\Delta}L$) and color (${\Delta}E$) in 'Gamhong' and 'Arisoo' showed lower than other cultivars while 'Arkansas black' was highest change of lightness and color as ${\Delta}L$ 8.27, ${\Delta}E$ 13.86 respectively. Total phenol and ascorbic acid contents in 'Arkansas black' were highest such as 334.3 mg GAE/100 g and 9.22 mg/100 g, respectively. And polyphenol oxidase (PPO) activity also was the highest such as 14.43unit in 'Arkansas black'. The correlation test showed browning of apple was significantly dependent on ${\Delta}L$, ${\Delta}E$, total phenol, ascorbic acid contents and PPO activity (p<0.01). On the contrary, no significant correlation had been determined between the browning and ascorbic acid oxidase (AAO) activity. Thus, it seemed that the browning of apple was not only due to oxidation of ascorbic acid but also total phenol. It was suggested that 'Gamhong' and 'Arisoo' were suitable for fresh cutting or minimal processing.