• 한국식품영양과학회지
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• 제35권4호
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• pp.410-415
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• 2006

청국장 분말이 고지방식이를 섭취한 흰쥐의 지질대사에 미치는 영향을 조사하고자, 성숙한(생후 20주령) 숫쥐를 4주간 고지방식이(0.5% 콜레스테롤, 10% 지방)로 고지혈증을 유도한 후 대조군(고지방식이), 청국장군(고지방식이에 단백질원으로 청국장 분말을 첨가한 식이) 및 상황버섯 청국장군(고지방식이에 단백질원으로 상황버섯 청국장 분말을 첨가한 식이 ) 등 3군으로 나누어 5주간 사육한 결과는 다음과 같다. 실험동물의 체중은 대조군이 41.8 g증가함에 비하여 청국장군 및 상황버섯 청국장군은 각 -3.7 g 및 -0.8 g으로 유의하게 감소되었고, 식이섭취량 및 식이효율은 대조군에 비하여 청국장군들이 유의하게 감소되었다. 간과 부고환지방의 무게 및 간조직의 콜레스테롤과 중성지질 농도는 대조군에 비하여 청국장군 및 상황버섯 청국장군이 유의하게 감소시키는 효과가 나타났다. 혈청의 중성지질, 총 콜레스테롤, LDL-콜레스테롤, VLDL-콜레스테롤 농도 및 동맥경화지수는 대조군에 비해 청국장군 및 상황버섯 청국장군이 유의하게 감소되었다. 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율은 대조군에 비해 청국장군들이 유의하게 증가되었다. 변의 무게, 총 지질, 중성지질 및 콜레스테롤 배설량은 청국장군들이 대조군보다 유의하게 증가된 것으로 나타났다. 이상의 결과로 보아 고지방식이에 청국장 및 상황버섯청국장 분말을 첨가 섭취시 혈청과 간조직의 중성지질, 총콜레스테롤과 LDL-콜레스테롤 농도 및 동맥 경화지수를 낮추고, 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율을 증가시키는 지질대사의 개선 효과가 나타났다.

• 대한한방소아과학회지
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• 제20권1호
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• pp.49-67
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• 2006

Objectives : The purpose of this study is to find out the effect of antioxidation related to aging of Cheongeumyeonsudan which is written on Dongui-bogam experimentally. Method : 14 weeks aged SD albino rats were separated into uncontrolled group, controlled group and CGY group. As controlled and CGY groups were induced aging by subcutaneous injection of D-galactose, at the same time we administered the extract of Cheongeumyeonsudan to CGY group for 6 weeks. After then we drew blood from each group, and took measurements; the activity of SOD, GSH-px, catalase in erythrocytes, TBARS value, concentration of total lipid, tryglycende, total cholesterol, HDL-cholesterol in blood plasma. Results : The activities of SOD, GSH-px in erythrocytes were significantly increased in the CGY group compared with control group. The activity of catalase showed a tendency to increase, but it was nor remarkable. The concentration of total lipid, the values of TBARS and total cholesterol was significantly decreased in the CGY group compared with control group, and the concentration of plasma HDL-cholesterol was not remarkable. The concentration of tryglycende in plasma showed a tendency to decreased. Conclusions : it is suggested that Cheongeumyeonsudan decreased the activities of free radical, the concentration of lipid in plasma and generate enzyme which form lipid peroxide.

• Journal of Nutrition and Health
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• 제23권7호
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• pp.467-476
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• 1990

This study was performed to investigate the effects of different fat diets on the serum lipid composition and on the morphology of liver tissue of the spontaneously hypertensive rats(SHR), Rats were fed for 17 weeks one of four fat diets, control(corn oil : beef tallow=1:1), beef tallow, grapeseed oil and soybean oil. Blood was withdrawn by heart puncture from each SHR, and used to determine the serum levels of total cholesterol, HDL-cholesterol, trigly ceride and phospholipid. No significant difference in body weight was observed in all diet groups. the total serum cholesterol was significantly lower in SHR fed grapeseed oil than that of other groups. The cholesterol lowering effect of grapeseed oil seemed to results from its high contents of phytosterol and linoleic acid. Also, serum HDL-cholesterol, triglyceride and phospholipid contents were low in all the groups. photochemical microscopic observations of the liver tissue revealed the congestion of sinosoid which is regarded as the characteristics of SHR, but no significant difference was observed among the groups.

• 한국응용과학기술학회지
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• 제3권2호
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• pp.9-16
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• 1986

The purpose of this study was to find the effect of dietary polyunsaturated fat on of vitamin levels E, total cholesterol and HDL-cholesterol in serum of Spraque-Dawley male rats. Experimental rats were fed for 4 weeks with corn oil, perilla oil, lard at a level of 10% and 20% respectively. The results obtained are as follows; 1. Body weight gains were similar to all groups and food intake was significantly lower in all experimental diet groups than control diet group, especially 20% corn oil diet group was the lowest among the experimental diet groups. 2. Vitamin E levels in serum were significantly lower in 20% corn oil and 10%, 20% perilla oil diets groups than control diet group. 3. Serum total cholesterol levels were significantly higher in 20% perilla oil diet group which was significantly lower in vitamin E level than control diet group, and serum total cholesterol levels of 10%, 20% lard diets groups were significantly higher than control diet group. 4. Serum HDL-cholesterol levels were significantly lower in 20% perilla oil diet group which was significantly lower in vitamin E level than control diet group, and serum HDL-cholesterol levels of 10%, 20% lard diets groups also were significantly lower than control diet group.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.883-889
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• 2008

Lipid peroxidation (LPO) has been identified as an important component of atherosclerosis. In this study, the effects of supplementation with cholesterol (0.5%), olive oil (5%) and vitamin E (0.05%) on erythrocyte glutathione (GSH), plasma malondialdehyde (MDA), total cholesterol, HDL-LDL cholesterol and triacylglycerol, brain and liver MDA and GSH concentrations of rats were investigated. A total of 50 Sprague-Dawley male rats aged 6 months, and of equal body weight were used and fed a standard ration ad libitum. Animals were housed in the University of Selcuk, Veterinary Faculty Experimental Animals Unit. The experiment lasted 60 days and there were five experimental groups as follows: 1. Control, 2. Cholesterol (0.5%), 3. Olive oil (5%), 4. Cholesterol plus vitamin E (0.05%), 5. Olive oil plus vitamin E (0.05%). At the end of the experiment, blood samples were taken by cardiac puncture and erythrocyte GSH, plasma MDA, cholesterol, HDL-LDL cholesterol, triacylglycerol and also GSH and MDA concentrations in brain and liver tissue of rats were spectrophotometrically determined. Supplementation of olive oil and cholesterol into rat diets (groups 2 and 3) caused significant differences in lipid parameters; HDL cholesterol concentrations were increased in the olive oil group and LDL cholesterol was lower than in the cholesterol fed group. Moreover, these decreases in LDL and triacylglycerol concentrations were more significant with vitamin E supplementation. The high plasma MDA concentrations showed that lipid peroxidation occurred in the olive oil group and the highest brain MDA concentrations were determined also in the olive oil group. These findings suggest that vitamin E addition may decrease the sensitivities of several oils to oxidation and that monounsaturated fatty acids in olive oil may decrease the incidence of atherosclerosis by regulating blood lipid profiles.

• 한국식품영양학회지
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• 제21권3호
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• pp.336-343
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• 2008

This study was conducted to investigate the effects of caffeine on lipid and mineral content in the serum of rats given a caffeine free diet(FC), a low caffeine diet(LC), a medium caffeine diet(MC), a high caffeine diet(HC) or a super-high caffeine diet(SHC) for 5 weeks. Thirty male Sprague-Dawley rats(body weight, $110{\pm}0.3$ g) were blocked into 5 groups and fed diets with or without pure caffeine. Caffeine intake models showed a lower mean-weight gain, food intake and food efficiency in the high caffeine diet groups(MC, HC and SHC groups) than the groups receiving a caffeine free or low caffeine diet(FC and LC groups). Serum total lipid, total cholesterol and LDL-cholesterol levels decreased, but the serum HDL-cholesterol level increased according to the increase in caffeine intake. Serum total lipid, HDL-cholesterol and serum triglyceride were significantly lower in the HC and SHC groups than the FC group. All of the serum minerals decreased as caffeine intake increased. Serum iron, calcium, magnesium and phosphorus significantly decreased in the HC and SHC groups compared to the FC group. Caffeine intake was associated with less weight gain and reduced serum total cholesterol, triglyceride and total lipid. The results suggest that rats fed high amounts of caffeine may be susceptible to osteoporosis due to their low levels of calcium, magnesium and phosphorus.

• 한국환경과학회지
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• 제26권7호
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• pp.875-879
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• 2017

We conducted two experiments to evaluate effects of feeding various types of red ginseng marc and Houttuynia cordata (H.cordata) on blood profiles of poultry in a completely randomized design. In experiment 1 (28 d), a total of 240 broilers (Arbor acres) were used. Four dietary treatments (15 broilers per pen with four replicate pens per treatment) were included: (1) control, (2) 2% red ginseng marc, (3) 1% fermented red ginseng marc with red koji, and (4) 2% liquid red ginseng. There were no significant differences in HDL and LDL-cholestrol among treatments (P > 0.05), but total cholesterol and triglyceride decreased in diets supplemented with red ginseng marc and 1% fermented red ginseng marc with red koji compared to that in the control treatment (P < 0.05). In experiment 2 (38 d), a total of 240 Pekin ducks were randomly divided into 4 groups by dietary treatments (control, 1% fermented H. cordata powder with red koji, 1% fermented H. cordata pelleted with red koji, and 1% fermented H. cordata coated with red koji) with 4 replicates of 15 ducks in each group. Total cholesterol and VLDL-cholesterol were not affected by diet with various types of H. cordata. However, increase in HDL-cholesterol and decrease in LDL and VLDL-cholesterol were greater in treatments with different types of H. cordata than in the control treatments (P < 0.05). In conclusion, using various types of red ginseng marc and H. cordata in poultry diets was effective for increasing HDL-cholesterol and decreasing total, LDL, and VLDL-cholestrol or triglyceride.

• 동아시아식생활학회지
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• 제6권1호
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• pp.41-49
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• 1996

This research was performed to Investigate the effects of green tea on the lipid composition of serum and liver and the specific activities of antioxidative enzymes. Male Sprague Dawley rats were fed 10% fat diet with lard and fish oil. Powdered green tea was added to the lard and fish oil diet at the level of 0.1% and 1%. After 6 weeks of feeding, serum and liver were obtained from experimental rats. Then we measured the concentration of total cholesterol, HDL-cholesterol and triglyceride. From liver cytosolic fraction, we analized the specific activities of superoxide dismutase, glutathione peroxidase and glutathione S-transferase. The level of total cholesterol and triglyceride were decreased and the ratio of HDL-cholesterol to total cholesterol was increased by the fish oil in the serum. But in the liver, the level of total cholesterol was increased by the fish oil and green tea than the lard. The specific activities of glutathione S-transferase were more increased in the fish oil than the lard. There was not effect of the green tea of daily dose on the lipid composition of serum and liver and the specific activities of antioxidative enzymes in rats.

• 대한약침학회지
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• 제6권1호
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• pp.61-66
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• 2003

In order to study the effects of Moschus Herbal-acupuncture on hyperlipidemia, we divided the rats into three groups(Normal group, Control group & Sample group) and performed the experimental research. Hyperlipidemia rats were induced by Triton WR-1339(150mg/kg) for 3days. Sample group was injected Moschus Herbal-acupuncture 0.1 ml/200g for 3 days and control group was injected equal dose of saline. And then we measured the amount of serum total cholesterol, triglyceride, phospholipid and HDL-cholesterol. The results were as follows : 1.Moschus Herbal-acupuncture showed decreasing effects on total cholesterol, triglyceride and phospholipid levels in serum significantly(p<0.05). 2.Moschus Herbal-acupuncture showed increasing effects on HDL-cholesterol level in serum significantly(p<0.05). According to the above results, Moschus Herbal-acupuncture showed significant decreasing effects on hyperlipidemia and it is considered that it is appropriate to apply for hyperlipidemia.

• BMB Reports
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• 제38권2호
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• pp.238-242
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• 2005

5-HT2A is one of major serotonin receptor that is involved in the action of serotonin-targeting drugs. Previous clinical studies have shown an unexpected association between lower cholesterol level and psychiatric diseases, in which T102C polymorphism of HTR2A, gene of 5-HT2A serotonin receptor, might be involved. Therefore, we hypothesized a potential association between lower cholesterol level and T102C polymorphism. The effect of the T102C polymorphism on the serum lipid profiles of 646 subjects without specific psychiatric disease was investigated. Genotype was determined by polymerase chain reaction and restriction fragment length polymorphism analysis. There were significantly lower levels of total cholesterol ($193.6{\pm}35.0$ versus $202.1{\pm}45.5\;mg/dl$, p = 0.016) and HDL-cholesterol ($42.7{\pm}11.6$ versus $46.3{\pm}12.7\;mg/dl$, p = 0.004) in CC genotype than non-CC genotypes. Moreover, multivariate analysis showed that the CC genotype is a strong predictor of a lower HDL-cholesterol level (p < 0.001). In conclusion, this study shows that the CC genotype of the HTR2A gene is related to lower HDL-cholesterol level in Koreans. This is the first demonstration showing the potential genetic relationship between the serotonin receptor gene polymorphism and the HDL-cholesterol level.