• 한국식품영양과학회지
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• 제43권11호
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• pp.1731-1736
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• 2014

산야초와 설탕의 비율을 1:1(w/w)로 혼합하는 전통적인 방법으로 달맞이꽃, 오행초, 옻의 발효액을 제조하여 상온에서 6개월간 발효 및 숙성 중 품질 특성, 효소 활성, 항산화 활성의 변화를 조사하였다. 모든 실험구에서 발효기간 중 당도는 감소하였으며, 점도는 발효 3개월까지 증가하다가 숙성기간 중 감소하였다. 3개월의 발효기간 중 pH는 낮아졌고 적정산도는 높아졌으나, 3개월의 숙성기간 중에는 뚜렷한 변화를 나타내지 않았다. 미생물의 변화는 발효 1개월 이후 6개월동안 점차 감소하는 경향을 나타내었으며, 유산균의 경우 전 기간 동안 검출되지 않았다. 전통적인 방법으로 제조한 산야초 발효액의 invertase, amylase, cellulase 활성은 발효 및 숙성 전 기간 동안 매우 낮은 활성을 나타내었고, 시중에 판매되고 있는 5종의 산야초 발효액 모두 매우 낮은 효소활성(1 unit 이하)을 나타내었다. 총 폴리페놀 함량은 발효기간 중 증가하다가 숙성기간 동안 일정하게 유지되었으며, 그중 달맞이꽃 발효액의 폴리페놀 함량(6.40 mg/mL)과 DPPH radical 소거능(40.52%)이 가장 높았다.

• 한국식품과학회지
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• 제42권5호
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• pp.571-577
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• 2010

본 연구에서는 예로부터 전해 내려오는 당뇨처방전에 사용되고 있는 문헌고찰을 토대로 6종의 약용식물(당귀, 백복령, 상백피, 상엽, 총목피 및 홍삼)의 항산화 및 항당뇨 활성을 비교 검토하고 이들의 상관관계를 검토하여 가장 효과적인 추출방법을 조사하였다. 당귀의 열수와 에탄올 추출물에서 각각 39.43와 37.75%로 다른 시료에 비하여 추출수율이 높았다. 폴리페놀 함량은 상백피 에탄올추출물에서 가장 높았고, 홍삼 초음파추출물에서 가장 낮았다. 플라보노이드 함량과 DPPH $RC_{50}$ 값은 상엽에탄올 추출물에서 가장 우수하였다. ${\alpha}$-Glucosidase저해활성은 상엽과 상백피 초음파추출물에서 높았고 추출방법 따른 저해율은 차이를 보였으나 세가지 추출물 모두 acarbose보다 높은 활성을 보였다. ${\alpha}$-Amylase저해활성은 상엽 에탄올추출물에서 가장 높은 저해활성을 보였고, 상백피와 백복령 에탄올추출물에서도 acarbose와 비슷한 결과를 보였다. 항산화 활성과 항당뇨 저해활성의 상관성은 에탄올 추출물과 초음파 추출물에서 유의적인 상관관계를 보였다. 에탄올 추출물에서 ${\alpha}$-glucosidase저해활성에 대한 폴리페놀 함량 또는 플라보노이드 함량과는 양의 상관관계($r^2$=0.792, 0.588)를 보였고, ${\alpha}$-amylase저해활성에 대한 플라보노이드 함량 또는 DPPH 소거능의 $RC_{50}$ 값에서도 음의 상관관계($r^2$=0.918, -0.594)를 나타내었다. 또한 ${\alpha}$-amylase 저해활성과 ${\alpha}$-glucosidase저해활성에서는 양의 상관관계($r^2$=0.746)를 보였다. 초음파 추출물에서는 ${\alpha}$-glucosidase저해활성에 대해 폴리페놀 함량과 플라보노이드 함량에서만 양의 상관관계($r^2$=0.763, 0.618)를 보였다. 항산화능에서의 상관관계를 살펴 본 결과 열수, 에탄올 및 초음파추출물에서 모두 폴리페놀 함량과 플라보노이드 함량, DPPH $RC_{50}$ 값과 유의적으로 높은 양의 상관관계가 있는 것으로 나타났다. 결론적으로 약용식물이 추출방법에 따른 항산화, 항당뇨 활성과 상관성에 차이를 보이고 있음을 알 수 있었다.

• Mycobiology
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• 제46권2호
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• pp.159-167
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• 2018

This study evaluated the in vitro and in vivo hypolipidemic effects of the medicinal mushroom Phellinus pini. The methanol extract (ME) of the fruiting body of Ph. pini was active against pancreatic lipase and cholesterol esterase with 99.14% and 67.23% inhibited activity at 1.0 mg/mL, respectively. It also inhibited 81.81% and 55.33% of ${\alpha}$-glucosidase and ${\alpha}$-amylase activities, respectively, at 2.0 mg/mL. Hyperlipidemia as induced by feeding rats with a high fat and cholesterol diet (HFC). HFC supplemented with a 5% fruiting body powder of Ph. pini (HFC + PhP) significantly reduced plasma total cholesterol, low-density lipoprotein cholesterol, and triglycerides in rats compared with HFC. The reduced levels were comparable to rats fed the normal control diet (NC). The atherogenic index of HFC + PhP rats was significantly lower than that of the HFC rats. The excretion of fecal total lipid and cholesterol in the HFC + PhP rats was significantly higher than those in the NC and HFC rats. Histopathological examinations demonstrated scant deposition of lipids in the liver of rats fed HFC + PhP. The dietary supplementation with the fruiting body powder provided natural plasma lipid and glucose lowering effects in experimental rats without adverse effects on the plasma biochemical parameters and liver function related enzyme activities. Therefore, the hypolipidemic effects of Ph. pini may be due to the inhibitory effects on pancreatic lipase, cholesterol esterase, ${\alpha}$-glucosidase, and ${\alpha}$-amylase, and excretion of excess lipids and cholesterol in the feces.

• 동아시아식생활학회지
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• 제19권1호
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• pp.96-104
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• 2009

Three kinds of grape yakju (Kyoho, Campbell and Moru) and grape-free control were fermented with rice using koji of Aspergillus kawachii as a fermenting agent, and their fermentation characteristics were investigated. For the three yakju, reducing sugar content decreased slowly over 2 days fermentation after an initial rapid decrease. In case of Brix, the reducing sugar content increased gradually after increasing rapidly during the first 2 days of fermentation. The Kyoho and Moru groups displayed a gradual increase in reducing sugar content after an initial 2-day decrease, and the sugar content of the Campbell group increased gradually during fermentation. Uniformly, pH increased gradually after an initial 2-day decrease, however total acid increased continuously throughout fermentation. Alcohol content of yakju showed almost no change after increasing until day 6 (Kyoho) of day 4 (others), with the control displaying the highest content. Control $\alpha$-amylase activity decreased gradually after an initial 2-day increase, while that of the three yakju decreased throughout fermentation. Activities of $\beta$-amylase activities paralleled the results of reducing sugar. Concerning total anthocyanin, the Moru and Campbell groups displayed a rapid increase until day 2, a decrease until day 12, and a gradual increase thereafter. While the Kyoho and control groups displayed virtually no change after an initial 2-day increase until day 12, after which a gradual increase also occurred. In concerning sensory evaluation, Moru yakju scored highest for violetness, flavor of grape, and sweet taste, and also displayed a low alcohol content. These attributed Moru yakju the preferred choice.

• 한국식품영양과학회지
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• 제13권1호
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• pp.64-70
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• 1984

감압 저장 중 tomato과실에서 일어나는 당전환의 일부를 조사 하고자 저장기간을 통하여 탄수화물의 성분과 그들에 관련된 효소활성 변화를 조사한 결과는 다음과 같다. tomato 과실에 함유되어 있는 soluble sugar의 대부분은 fructose glucose이며 소량의 sucrose와 maltose가 존재하였다. 이들의 저장 중 변화를 살펴보면 fructose는 저장전반에 걸쳐 증가하는 경향이었고, glucose는 저장초기에 증가하다가 저장말기에 약간 감소하였으며 sucrose는 저장시일이 경과함에 따라 급격히 감소하는 경향이었다. 당전환에 중요한 역할을 하는 ${\alpha}-amylase$와 in-vertase의 활성은 climacteric onset 시기에 급격히 증가하였으며 이로 인하여 이 시기에 total soluble sugar는 급격히 증가하는 반면 기질인 starch와 sucrose는 현저히 감소하는 경향이었다. 이러한 경향은 압력에 의한 영향보다 온도에 따른 영향이 더 크게 나타났다.

• Applied Biological Chemistry
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• 제40권5호
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• pp.433-437
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• 1997

대추 첨가량을 달리하여 대추술을 제조하고 경시적인 발효 특성을 분석하였다. 품온은 모든 처리구가 담금 2일에 $24{\sim}26^{\circ}C$로 가장 높았고, 총산은 발효 경과기간과 대추의 첨가량이 증가함에 따라 서서히 증가하였다. 환원당과 전당은 모두 2단 담금에서 대추액을 첨가한 후에 각각 $13{\sim}53%$ 및 $43{\sim}51%$로 최대가 되었다가 계속 감소하였고 대추의 첨가량이 많을 수록 높았다. 알코올은 발효가 진행됨에 따라 증가하였는데, 특히 발효 1-3일째에 급격한 증가를 보였고 대체로 대추첨가량이 많을수록 증가율이 높게 나타났다. Amylase활성은 2-2.5일에 최대 활성을 보인 후 계속 감소하였다. 대추술의 전체적인 품질은 2그룹으로 구분되었으며 E가 가장 높게 나타났고 A와 F가 가장 낮았다.

• 한국응용과학기술학회지
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• 제4권2호
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• pp.57-62
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• 1987

To investigate the effect of feeding rats medium-chain triglyceride(MCT), triglyceride containing primarily $C_8$ and $C_10$ fatty acids, it were compared to the effects of feeding triglycerides composed of long-chain triglyceride (LCT), corn oil and lard, on the serum enzyme activity. For 4 weeks rats fed a diet containing 20％ MCT or LCT ${\cdot}$ MCT, as compared with LCT, had the following effects: 1) The total lactic dehydrogenase(LDH) activities in the serum of all experimental group were significantly decreased then those of control group. 2) The activities of glutamic oxaloacetic transaminase (GOT) in the serum of all experimental group were decreased than those of control group. 3) The activities of glutamic pyruvic transaminase (GPT) in the serum of all experimental group were decreased, MCT and LCT group were singinficantly decreased than of control group. 4) The activities of ${\alpha}-amylase$ in the serum of all groups were significantly increased than those of control group. 5) According to electrophoresis, LDH of LDH isoezyme activities in MCT and Lard group were increased with those of $LDH_5$ in corn oil group were increased than those of control gourp. It is suggested that the MCT and LCT fed to rats influence on the activity of various serum enzymes.

• Asian-Australasian Journal of Animal Sciences
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• 제13권11호
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• pp.1508-1513
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• 2000

The aim of this trial was to study the response of laboratory animals including omnivores (rats) and herbivores (rabbits, guinea pigs and hamsters) to the same level of dietary fiber on their digestive enzymes and hindgut fermentation. Ten weanling animals of each of four species, rabbits, guinea-pigs, rats and Syrian hamster, were fed a basal diet of 18% crude protein and 10% crude fiber for six weeks. The digesta and tissue of each intestinal segment were collected to measure the activity of digestive enzymes. Rabbits contained the highest secreted pepsin activity in the stomach, whereas rats contained the highest protease and ${\alpha}-amylase$ activity in the small intestine, and lower fibrous hydrolases in the hindgut than rabbits, guinea pigs and hamsters. The total VFA productions in the caecum and colon were highest in rats, followed by hamsters and rabbits, while the guinea pigs contained the lowest VFA and a different pattern of VFA molar ratio from the other laboratory animals. The degree of hindgut fermentation in these laboratory animals was in reverse to the trend for their fiber digestion.

• 한국응용과학기술학회지
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• 제1권1호
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• pp.23-31
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• 1984

In order to investigate the acute toxicity of autoxidized methyl linoleate(AOML) on the activity of serum enzymes in the mouse, we administered once 0.45ml of AOML to ICR strain mouse by using stomach tube. The following results were obtained: The total lactic dehydrogenase(LDH) activities in the serum of AOML group were generally increased than those of normal group. According to electrophoresis, the activities of LDH, were increased while those of LDH, were decreased. The activities of glutamic oxaloacetic transaminase(GOT), glutamic-pyruvic transaminase(GPT) and ${\alpha}-amylase$ in the serum of AOML group were increased more than those of normal group. The activities of alkaline phosphatase in the serum of AOML group were increased but those of isozyme were not confirmed in the normal and AOML group. In the serum protein of AOML group, albumin was increased, on the other hand ${\gamma}-globulin$ was decreased. At the peripheral blood slide smear, lymphocytes were significantly decreased but neutrophils were increased and the morphological change of erythrocytes was observed. From these results we conclude that the AOML fed to mouse influences on the activity of various serum enzymes and blood cells in the mouse.

• 동아시아식생활학회지
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• 제14권4호
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• pp.363-369
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• 2004

Three different doengjang(fermented soybean paste) were prepared by using B. subtilis SS103 with high angiotensin converting enzyme(ACE) inhibitory activity, Asp. oryzae and mixed culture of two organisms, respectively. Quality characteristics and the inhibitory activity were compared. $\beta$-Amylase activity rapidly increased during 30 days of fermentation, but lower activity was found in doenjang prepared with B. subtilis. However, $\alpha$-Galactosidase activity decreased for 15 days of fermentation and little activity was observed after that fermentation period. Protease production tended to increase during the fermentation. High protease activity was found only in doengjang prepared with Asp. oryzae, but the doengjang prepared with B. subtilis SS103 showed very low activity. Total isoflavone content at 90 day fermentation was the highest in doengjang prepared with mixture of Asp. oryzae and B. subtilis. ACE inhibitory activity increased during the fermentation period. The highest activity was found in doengjang made with mixture of Asp. oryzae and B. subtilis. Sensory evaluation on doengjang itself and its soup indicated that doengjang made with mixture of Asp. oryzae and B. subtilis showed higher acceptability on taste, flavor and color than those prepared with only Asp. oryzae or B. subtilis, respectively.