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Investigation of Root Morphological and Architectural Traits in Adzuki Bean (Vigna angularis) Cultivars Using Imagery Data

  • Tripathi, Pooja;Kim, Yoonha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.67 no.1
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    • pp.67-75
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    • 2022
  • Roots play important roles in water and nutrient uptake and in response to various environmental stresses. Investigating diversification of cultivars through root phenotyping is important for crop improvement in adzuki beans. Therefore, we analyzed the morphological and architectural root traits of 22 adzuki bean cultivars using 2-dimensional (2D) root imaging. Plants were grown in plastic tubes [6 cm (diameter) × 40 cm (height)] in a greenhouse from July 25th to August 28th. When the plants reached the 2nd or 3rd trifoliate leaf stage, the roots were removed and washed with tap water to remove soil particles. Clean root samples were scanned, and the scanned images were analyzed using the WinRHIZO Pro software. The cultivars were analyzed based on six root phenotypes [total root length (TRL), surface area (SA), average diameter (AD), and number of tips (NT) were included as root morphological traits (RMT); and link average length (LAL) and link average diameter (LAD) were included as root architectural traits (RAT)]. According to the analysis of variance (ANOVA), a significant difference was observed between the cultivars for all root morphological traits. Distribution analysis demonstrated that all root traits except LAL followed a normally distributed curve. In the correlation test, the most important morphological trait, TRL, showed a strong positive correlation with SA (r = 0.97***) and NT (r = 0.94***). In comparison, between RMT and RAT, TRL showed a significantly negative correlation with LAL (r = -0.50***); however, TRL did not show a correlation with LAD. Based on RMT and RAT, we identified the cultivars that ranked 5% from the top and bottom. In particular, the cultivar "IT 236657" showed the highest TRL, SA, and NT, while the cultivar "IT 236169" showed the lowest values for TRL, SA, and NT. In addition, the coefficient of variance for the six tested root traits ranged from (14.26-40%) which suggested statistical variability in root phenotypes among the 22 adzuki bean varieties. Thus, this study will help to select target root traits for the adzuki bean breeding program in the future, generating climate-resilient adzuki beans, especially for drought stress, and may be useful for developing biotic and abiotic stress-tolerant cultivars based on better root trait attributes.

Parametric Study for Seismic Design of Temporary Retaining Structure in a Deep Excavation by Dynamic Numerical Analysis (동적수치해석을 이용한 대심도 흙막이 가시설 내진설계 변수연구)

  • Yang, Eui-Kyu;Yu, Sang-Hwa;Kim, Dongchan;Kim, Jongkwan;Ha, Ik-Soo;Han, Jin-Tae
    • Journal of the Korean Geotechnical Society
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    • v.38 no.12
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    • pp.45-65
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    • 2022
  • In this paper, a diaphragm wall that supports soils and rock was modeled using FLAC, a finite difference analysis program, to evaluate the seismic behavior of temporary retaining structures in a deep excavation. The appropriateness of the numerical model was verified by comparing its results with those of the centrifuge test performed in a similar condition. The bending moment distribution along the diaphragm wall shows a very similar tendency, and the maximum acceleration obtained at the backfill and top of the wall shows a difference within 5%. Based on the developed model, a parametric study was conducted in various input earthquake, ground, and excavation conditions. The maximum structural forces and bending moment under earthquake loading were compared with the maximum values during excavation, from which the critical condition that requires a seismic design was roughly sorted out. The maximum bending moment of a wall that retains soil layers increased 17%. Particularly, the axial force of struts located in loose soils increased 32% under 100 years return period of an earthquake event, which strongly is estimated to require seismic design for structural safety.

Field Evaluation of Traffic Wandering Effect on Asphalt Pavement Responses (차량의 횡방향 주행이격에 의한 아스팔트 콘크리트 포장의 응답특성 분석)

  • Seo, Youngguk;Kwon, Soon-Min;Lee, Jae-Hoon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.3D
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    • pp.453-459
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    • 2006
  • This paper presents an experimental evaluation of wandering effect on asphalt concrete pavement responses. A laser-based wandering system has been developed and its performance is verified under various field conditions. The portable wandering system composed of two laser sensors with Position Sensitive Devices can allow one to measure the distance between laser sensors and tire edges of moving vehicle. Therefore, lateral position of each wheel on the pavement can be determined in a real time manner. Pavement responses due to different loading paths are investigated using a roll over test which is carried out on one of asphalt surfaced pavements in the Korea Highway Corporation test road. The pavement section (A5) consists of 5 cm thick surface course; 7 cm intermediate course; and 18 mm base course, and is heavily instrumented with strain gauges, vertical soil pressure cells and thermo-couples. From the center of wheel paths, seven equally-spaced lateral loading paths are carefully selected over an 140 cm wandering zone. Test results show that lateral horizontal strains in both surface and intermediate courses are mostly compressive right under the loading path and tensile strains start to develop as the loading offset becomes 40 cm from the wheel path. The development of the vertical stresses in the top layers of subbase and anti-frost is found to be minimal once the loading offset becomes 50 cm.

Nitrogen Recovery and Application Method in a Satsuma Mandarins Orchard (온주밀감 과원 토양에서 질소에 대한 시비방법과 시비수준에 따른 회수율)

  • Kang, Young-Kil;U, Zang-Kual;Kang, Bong-Kyoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.2
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    • pp.143-150
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    • 1998
  • In order to evaluate the nitrogen (N) balance, from the different application methods and levels of $^{15}N$ applied to a satsuma mandarin orchard soils in spring, we surface-applied N as urea at the rates of 50 (water-dissolved), 100 (solid and water-dissolved) and 150% (solid) of the recommended rate ($180kg\;ha^{-1}$) in spring (lebeled N), summer (nonlebeled N) with application ratio of 5:2:3. Fruit yield and quality were not significantly affected by any treatment. Nitrogen contents of spring flush leaves in late August were 3.0% regardless of the treatments. The N recovery by parts of tree itself was in the order of leaves, fruits, roots, stems, and the highest recovery per tree was 22.3% in the 50% recommended water-dissolved surface broadcast while there were not much differences for N recovery (11.9 to 13.6%) among the other three treatments. Total N content in top 30cm of soils was 0.47% regardless of the treatments, but N proportion and total residual N from the fertilizer applied increased with increasing N rate while the N recovery in soils decreased. For the recommended N rate, N proportion and the residual N from the fertilizer applied were greater in the water-dissolved surface broadcast than those in soils surface broadcast. The highest total (tree + soils) N recovery was 70.9% in the 50% recommended water-dissolved surface broadcast, but tended to decrease to 52.2, 46.6, and 43.2% for the recommended water-dissolved surface broadcast, 100 and 150% of the recommended solid surface broadcast, respectively.

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A Study of Sulfur Nutrition on the Flavour Components of Garlic (Allium sativum L.) (유황성분(硫黃成分)이 마늘의 향기성분(香氣成分)에 미치는 영향(影響))

  • Chang, Ki Woon;Hwang, Joon Young;Woo, In Shik
    • Korean Journal of Soil Science and Fertilizer
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    • v.21 no.2
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    • pp.183-193
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    • 1988
  • The relationship between the sulfur fertilization, and the quality and yield of garlic was investigated. The garlic was grown in pot and field at the five fertilization levels of sulfur. The growth characteristics, yield and flavour components of the garlic were studied. The results were summarized as follows; 1. The sulfur applications contributed to a weight and yield of the bulb in SP-10 (10kg/10a) and SF-20 (20kg/10a) significantly, respectively. 2. From the volatile oil obtained by steam distillation of garlic, fourteen organie sulfur components were separated, identified and quantified by GC and GC/MASS and the SP-10 and SF-20 were higher in the concentration of the flavour components in the volatile oil than other treatments. 3. It is considered that the organic sulfur components were synthesized in the top and transported to the bulb of the garlic. 4. In conclusion, SF-20, 20kg/sulfur per 10a, could be recommended to get maximum yield and a good quality of garlic in view of evaluation through the contents of organic sulfur components in the volatile oil and the yield of the bulb.

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Physiological Respone of Rice Plant Enviromental Stress II Effect of low temperature on the contents of chlorophyll, nitrogen and potassium m leading local and IR667 (환경장애(環境障碍)에 대(對)한 수도(水稻)의 생리반응(生理反應) II IR667계통(系統)과 장려품종(奬勵品種)의 엽녹소질소(葉綠素窒素) 및 가리함량(加里含量)에 대(對)한 저온(低溫)의 영향(影響))

  • Park, Hoon;Kim, Young Woo;Kim, Yung Sup
    • Korean Journal of Soil Science and Fertilizer
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    • v.6 no.4
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    • pp.231-237
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    • 1973
  • Eeffect of temperature (3-day or 7-day treatment under $15^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$) on chlorophyll, nitrogen and potassium content in the second leaf blade from top of IR667 line (Suwon 213 and 214) and leading local varieties (Jinheung and Paldal) was investigated at the end of nursery, maximum tillering and flowering stage using phytotron. 1. Chlorophyll content was higher in IR667 line than in the local at $25^{\circ}C$ but reversed at $20^{\circ}C$ or $15^{\circ}C$ and chlorophyll a/b value was always high in the local. 2. Chlorophyll content per fresh weight decreased with growth and low temperature effect was greater at the end of nursery and on chlorophyll a than on chlorophyll b. 3. Chlorophyll a/b value increases with the increase of chlorophyll content and the increasing rate of chl. a/b value per chlorophyll increment tends to decrease under unfavorable condition. This decrease is greater in low temperature sensitive IR667 than local varieties. 4. According to chlorophyll retention value IR667 line was weaker at low temperature. 5. The content of total nitrogen or soluble nitrogen (methanol soluble) was decreased by low temperature. 6. Chl. (a+b)/S-N value decreased with growth and seemed not to be greatly affected by temperature and always higher in the local. 7. Potassium content (total or methanol soluble) tends to decrease at low temperature and soluble K increased with chlorophyll content. 8. High yielding ability of IR667 seems to be attributable to its high chlorophyll content at high temperature and easy leaf discoloration by low temperature or by nitrogen depression of IR667 seems to be attributable to the low Chl./S-N value.

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Effects of Simulated Acid Rain on the Growth of Pinus rigida × taeda Seedlings Inoculated with Ectomycorrhizal Fungi, Pisolithus tinctorius and Suillus luteus (인공산성우(人工酸性雨)가 모래밭버섯과 비단그물버섯 균근균(菌根菌)으로 접종(接種)한 리기테다소나무 묘목(苗木)의 생장(生長)에 미치는 효과(效果))

  • Ko, Min Gyoo;Lee, Kyung Joon
    • Journal of Korean Society of Forest Science
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    • v.77 no.4
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    • pp.453-459
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    • 1988
  • The purposes of this study were to evaluate the effects of acid rain on tree growth and on the mycorrhizal formation and the effects of mycorrhizae on the host tolerance to acid rain. Simulated acid rain was applied for five months to Pinus $rigida{\times}taeda$ seedlings in pots inoculated with Pisolithus tinctorius (Pt) and Suillus luteus (Sl). Mycelial inocula of Pt and Sl were either mixed with entire pot soil (Mix) or casted as a band (Band) after soil sterilization. Three pH levels of acid rain (pH 3.0, 4.5 and 6.4 adjusted by 3 : 1 mixture of sulfuric and nitric acids) were tested. Pt-Mix was most effective in growth stimulation and resulted in 45-90% increase in tree height in sandy loam. Pt-Band was less effective than Pt-Mix in growth stimulation and mycorrhizal formation. Simulated acid rain at pH 4.5 stimulated height growth by 10-55%, while acid rain at pH 3.0 did not significantly affect the height growth. The top/root ratio was increased by pH 4.5 treatment, while pH 3.0 treatment reduced it. Mycorrhizal infection rate was not affected by acid rain. Pt inoculation reduced acid-induced leaf injury by 28-58% in both pH 3.0 and 4.5 compared with un-inoculated plants. Sl was also effective in growth enhancement, but was less effective than Pt in both mycorrhizal infection and reducing leaf injury.

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Research on the Effect of the Control Methods of Irrigation Water on the Growth and Yield of Paddy Rice. (한발기에 있어서 용수관리 방법이 수도생육과 그 수량에 미치는 영향에 관한 연구)

  • 김시원
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.13 no.1
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    • pp.2177-2190
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    • 1971
  • This experiment was made to determienthe effect of various soil moisture contents in simulated drought conditions on different stages of rice growth. The drought conditions were developed at such three rice-growing stages as transplanting, immediately after transplanting and young ear forming. Three different lengths of drought periods, which are ten days, twenty days and thirty days, were applied for each growing stage of rice. The rice variety used this experiment is Nong-rim 29. This experiment was conducted at the university farm of the Kon-Kuk University during the period of $1968{\sim}1970$. Three reprications for each of 12 treatments and split plot design were employed in this study. Bottomless wood square boxes, $1^m{\times}1^m{\times}1^m$, were burried in the test plot and box top was covered with poloyethylene sheets to avoid natural rainfall drops. Standard plots were irrigated continuously with a water depth of 40mm/day and those of drought treatments were irrigated continuously up to the beginning of the planned drought period, and they were irrigated again with a depth of 40mm/day up to the maturing stage of rice. Other methods for rice raising followed those methods developed by the Field Crops Experiment Station of the Office of Rural Development. During this experiments, climatic conditions in regard to rainfalls, sunshine hours, and temperatures were observed. According to this observation, those values measured deviate slightly from the annual means. However the growing condition of rice plants were normal. The pH value of irritation water is nearly neutral, and soils in the test plots are relatively fertile, being similar to ordinary paddy soils. Analysis of variances for number of stalks, plan-height, ear sprouting date, length of stalks, ear length, number of ears per plant, fertility, grain weitght, weight of plant, and yield were carried out. The variances for plant height, ear sprouting date, length of stalk ear length, and yield has statistical significance under drought treatments applied at three different growing stages. The variance showing the effect of lengths of drought period is highly significant for all the treatments studied except that of grain weight. The interaction between drought periods and drought treatments at different growing stages is significant for plant height, stalk length, ear length, number of ears, fertility and yield, these results indicated that droughts at different growing stages have influence on plant height, ear length, yield, and length of drought period also has strong influence on all factors studied except grain weight. The combination of drought treatments at different rice growing stages and lengths of drought periods has different effects on various agronomic characteristics, including yield. Plant height under drought treatment practiced at transplanting stage is the lowest, and drought treatment applied immediately after transplanting resulted in the least number of stalks. The effect of different lengths of drought periods on plant height and number of stalks depends signis ficantly on increasing days of drought. Ear sprouting date tends to be delayed for one or two days undedrought treatments at transplanting period and with increasing days of drought. Better yield is secured in drought treatment applied immediately after transplanting. Adverse effect war observed when drought treatment was applied at ear forming period. These effects may be attributed to the alternation of irrigation and drought causing vigorous root activity. In general, yield linearly decreases as the length of the drought period increases. The results obtained in this study demonstrate that, in order to mimimize damage due to drought, and, to save irrigation water, paddy fields, immediately after transplanting, may be not irrigated, since there is sufficient moisture in the soil, and that sufficient irrigation water should be applied again in the ear forming stage of rice plant.

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Physicochemical Changes of Food Waste Slurry Co-fermented with Pig Manure Slurry (음식물쓰레기와 돈분 액상물의 혼합부숙시 이화학적 특성 변화)

  • So, Kyu-Ho;Seong, Ki-Seog;Hong, Seung-Gil
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.4
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    • pp.242-248
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    • 2007
  • To find a feasibility of utilization of food waste slurry (FWS) generated during composting, FWS was combined with pig manure slurry (PMS) in various ratios and the change of nutrient contents and offensive odor of the combined slurries before and after fermentation were studied. The initial pH was 7.67 for PMS and 8.45 for FWS. However, during the fermentation, pH increased in the combined slurries with the higher FWS rate among the treatments while decreased in thosewith higher PMS rate. EC of each slurry sample showed that the difference among combined slurry samples has been reduced during fermentation and became stabilized in $21{\sim}23dS\;m^{-1}$ after 180 days. After 180 days fermentation, total nitrogen (T-N) decreased. T-N of mixture with a half and more FWS decreased up to 0.1%, less than the critical level (0.3%). The contents of O.M., T-N, phosphorus, calcium and magnesium decreased with fermentation while those of potash and salinity increased. From initial fermentation until 30 days, a lot of $NH_3$, as an offensive odor, was produced. However, it decreased steadily, except in higher PMS rate. In terms of producing $50{\mu}g\;ml^{-1}$ of $NH_3$, the top layer took 30 days after fertilization with FWS only, 45 days for utilized treatment with F75 (25 % of PMS), 75 days for utilized with F50 (50%) and F25 (75%) and 90 days for PMS only, respectively. $RNH_2$ also had similar trend with $NH_3$ but it was produced continuously as long fermentation proceeded. In terms of $RNH_2$, the decrease in concentration up to $50{\mu}g\;ml^{-1}$ were; 45 days for FWS only(F100), 105 days for F75 utilization, 120 daysfor F50, 165 days for F25, respectively. ethyl mercaptan was produced in PMS until 180 days after fertilization but it was not produced in FWS. Sensory tests as an integrated test of offensive odor were also done. FWS showed lower than 1 after 30 days from initial fermentation, while PMS had still offensive odor even up to 180 days from initial fermentation. It is probably affected by the continuous production of ethyl mercaptan and amines. However, considering in decrease T-N content caused by volatilization while offensive odor intensity according to official standard of fertilizer is lower than 2. Further study on controlling offensive odor needs to be done.

Changing Aspects of the Wall Types of Hahoe Village (하회마을 담장 형태의 변화양상)

  • Kim, Dong-Hyun;Lee, Won-Ho
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.5
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    • pp.87-96
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    • 2017
  • This study focuses on the Andong Hahoe Village and seeks to identify the shape of the walls since the 1970s. The change of walls can be divided into four periods based on characteristics of materials, shape and distribution. The following is a summary of the results: First, In the 1970s, when Andong Hahoe Village was not designated as a cultural heritage, roof tiles hung on the earthen walls in the middle of the village were major forms. On the outside of the village, rice straw and pine needles were put on the earthen walls or bush clover walls were put in place around if walls were not built. Second, after being designated as a cultural heritage in the 1980s, readjustments for cultural heritages were carried out at the primary stage. However, the distribution of cultural heritages and major changes were not determined at this time since readjustments were mainly focused on the renovation of derelict houses or maintenance of infrastructures. Third, in the past the use of stone bricks for the Hahoe Village site had been difficult, but in the 1990s, replacements with soil-stone walls were identified and the usage of roof tiles increased. The portion of earthen walls, which used to be the major form in the prior era, decreased and this seems to have continued until the 2000s. Fourth, via a field survey, it was found that most of Hahoe village walls consisted of soil cement bricks mixed with cement, steel, lime, gravel. etc. Also, the scope of straw-stricken walls and bush clover walls were reduced to a section of area outside of the village. Fifth, from the 1970s to the present, there were changes to the walls in Hahoe Village including an increase in usages of new materials and an expansion of houses with tiled roofs on top in accordance with the replacement of walls of existing houses. Relevant reasons for this have been identified, such as the fading value of Fungsui(風水) and lack of original records, insufficient awareness and expertise in non-building areas, and the relationship between residents on repairing the wall.