• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.222-229
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• 2022

Apples are the most grown fruit crops in the fruit industry of Korea. However, virus or viroid infection such as apple mosaic virus (ApMV), apple stem grooving capillovirus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV), apple scar skin viroid (ASSVd) causes fruit yield reduction and poor fruit quality. Therefore, in this study, we examined to established an efficient virus-free system to eliminate the most infected ASGV virus in domestic apple orchard. We investigated that the shoot growth rate and the virus removal rate in ASGV infected potted apples that were treated with heat treatment in a growth chamber (constant temperature/humidity device) maintained at 36℃, 38℃ and 40℃ for 4 weeks. Here we found that the shoot growth rate was the highest in the heat treatment group (36℃) and the virus was removed in the middle and top of the shoot but not in the bottom. The virus was did not removed in the 38℃ and 40℃ heat treatment group in all section of shoots, and the heat treatment group (40℃) died after 4 weeks of heat treatment without growth of shoots. We performed in vivo shoot-tip grafting using the shoot-tip of potted apple heat-treated at 36 ℃, and we also investigated the viability and virus removal rate, which showed 94% viability and 20% virus removal rate. Collectively, our results suggest that it would be possible to produce the virus-free apple plants through heat treatment and shoot-tip grafting.

• Korean Journal of Agricultural Science
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• v.43 no.1
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• pp.21-27
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• 2016

Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) have been known to induce top working disease causing economical damage in apple. Occurrences of these three viruses in pome fruit trees, including apple, have been reported around the world. The transmission of the three viruses was reported by grafting, and there was no report of transmission through mechanical contact, insect vector, or seed except some herbaceous hosts of ASGV. As RNA extraction methods for fruit trees, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and multiplex RT-PCR techniques have been improved for reliability and stability, and low titer viruses that could not be detected in the past have become detectable. We studied the seed transmission ability of three apple viruses through apple seedling diagnosis using RT-PCR. Nineteen seeds obtained from commercially grown apple were germinated and two of the resulting plants were ASGV positive. Seven clones of the amplified ASGV coat protein (CP) genes of these isolates were sequenced. Overall sequence identities were 99.84% (nucleotide) and 99.76% (amino acid). Presence of a previously unreported single nucleotide and amino acid variation conserved in all of these clones suggests a possible association with seed transmission of these 'S' isolates. A phylogenetic tree constructed using ASGV CP nucleotide sequences showed that isolate S sequences were grouped with Korean, Chinese, Indian isolates from apple and Indian isolates from kiwi.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.637-643
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• 2010

For enhancement of apple productivity, the calcium fertilizers to increase the calcium content of fruit, and reduce the incidence of bitter pit in apples are applied. Crushed oyster shell contains a plenty of calcium carbonate, and a very small amount of boron, ferrous, and manganese. A field study was conducted to determine the optimum level of crushed oyster shell for soil nutrient management, and its effect on the induction of bitter pit in cultivar 'Gamhong' apple. The application of crushed oyster shell increased organic matter, available phosphate, and exchangeable cation concentration in soil. We found a significant positive correlation between soil pH, and application level of crushed oyster shell in both top, and subsoils. The incidence of bitter pit in apple fruit at the harvest stage was significantly higher in non treatment and calciummagnesium carbonate treatment than on over applications 4 Mg $ha^{-1}$ for crushed oyster shell. However, the optimum level of crushed oyster shell was 2 Mg $ha^{-1}$, especially considering the soil Ca content, soil pH, fruits weight, and yield. Our results show that the crushed oyster shell can be effective in restoring the soil nutrient balance, and inducing the bitter pit in apple fruit.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.366-370
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• 2013

The effects of the early tree shapes with renewal by top-working on Y-shaped peach tree, and the influence on the maintaining fruit quantity with gradual renewal of interstock cultivar were determined. In the comparison of the places of top-working tree, top working tree on the inside of the main branch of interstock cultivar had higher graft union rate and branch growth than those of top working tree on the outside. Tree width, basal diameter of shoot, and number of bearing shoots were smaller in top working tree than in replanted tree. Although labor time was not different to control top working tree and replanted tree, labor time was much required to manage top working tree with interstock cultivar. Accumulated fruit production was 2,384 kg/10a in top working tree and 2,025 kg/10a in replanted tree for three years. However, top working tree had no loss of fruits because interstock cultivar of top-working tree had 3,727 kg/10a of fruits. No variation on fruit quality was observed between top working tree and replanting tree. In terms of economic value of top-working tree, labor to manage interstock cultivar, fertilizer price, fruit bagging, and grading and packing price increased. However, fruit production increased, and price of seedling, rental equipment, pulling-out trees, and repairing supporting system decreased. Therefore, gradual renewal of topworking tree has effects on the maintaining fruit quantity, supplementation on fruit loss, and renewal cultivar.

• The Korean Journal of Pesticide Science
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• v.5 no.4
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• pp.40-44
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• 2001

Pesticide actual usage in fruit crop cultivation was surveyed, and usage trends of individual pesticides were evaluated to provide data for the development of indicators of environmental impact. The amount of pesticide used for fruit crops indicated the order of fungicide>insecticide>herbicide unlike the case of paddy rice. The fungicide rate of total usage was 72% in apple cultivation. Top ranking fungicides used on fruits were Mancozeb, Propineb, Thiophanate-methyl and the main insecticides were Mancozeb, Propineb, Thiophanate-methyl. The usuage by formulation types showed the order of WP>EC>SL>SC>SP>WG. Pesticide usage (a.i.) per hectare by different fruits was citrus 48.6kg, apple 27.1kg, pear 18.6kg, persimmon 17.5kg, peach 11.3kg and grape 9.2kg. Comparison of pesticide usage (a.i.,kg/ha) in some fruit crops between Korea and USA indicated that more pesticides were used for citrus, pear and peach in USA than Korea while more pesticides were used in Korea than USA for apple.

• Food Science and Preservation
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• v.16 no.6
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• pp.817-823
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• 2009

We investigated the effects of storage temperature and packaging treatment on the freshness of high-quality 'Fuji' apples to improve consumer confidence in the maintenance of high fruit quality during distribution. A 0.35 mm-deep PET tray form-sealed with a 0.05 mm LDPE film lid was developed and tested with the aim of optimizing gas composition within the package headspace to utilize potential modified atmosphere (MA) storage to maintain the freshness of apples. Weight loss, color difference, firmness, respiration rate, gas concentrations in packages, acidity, solid soluble content, and fruit decay rate were measured during storage at $5^{\circ}C$ and $25^{\circ}C$. The results showed that respiration rate, weight loss, color difference, and firmness were lower and overall quality better during storage at $5^{\circ}C$ compared with $25^{\circ}C$. Also, the fruits packed in the functional tray form showed a low level of quality changes compared with the control (no packaging). The accumulated gasconcentrations in the headspace of the packages decreased from 21% to 12% $O_2$ and increased from 0% to 5% $CO_2$ (v/v) on day 7, and after remained at those levels thereafter. Soluble solid contents and total acidities of the packaged fruits were in the range of $11\;-\;14^{\circ}Brix$ and 0.2 0.3% during storage. Decay rates in the control and packaged fruits were more than 20% and less than 10% at 3 weeks, respectively. Based on the standard acceptable level (less than 10%) of total weight loss, it could be estimated that the shelf life of top-quality fruits packed in functional trays was 3 weeks at $5^{\circ}C$ and 16 weeks at $25^{\circ}C$, whereas the shelf life of the control fruit was 1 week and 12 weeks, respectively. For the top-quality fruit "Fuji" apples, the best results were obtained with a functional tray form for packaging treatment and a storage temperature of $5^{\circ}C$.

• Current Research on Agriculture and Life Sciences
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• v.6
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• pp.163-169
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• 1988

This study was conducted to predict the density changes according to concentration and temperature changes under freezing point. This information is needed for the design of freezing equipment and for the efficient utilization of refrigerating system. Orange juice, Apple juice, Grape juice and Sucrose solution were used for the measurement of density in this study at the temperature range from $-5^{\circ}C$ to $-40^{\circ}C$ and at the concentration range from 10 to 40%. The unfrozen water fraction of samples was determined by Heldman's method. The density values were determined by measuring the weight of a frozen solution at each temperature with a known volume. Solutions were placed in the thick-walled aluminum tubes. When the solution was frozen the excess ice was removed with a razor until the surface of the ice was flush with the top of the aluminum tube. The tube and ice were weighted immediately. Knowing the volume, tare weight, and final weight, the density could be determined. With this procedure, the data of density and unfrozen water fraction for fruit juices and sucrose solution were collected. The density prediction models of fruit juices and sucrose solution under freezing point were established by the optimization computer program with measured experimental data.

• Journal of the Ergonomics Society of Korea
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• v.35 no.5
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• pp.383-389
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• 2016

Objective: In this paper, work time for the representative fruit crops are collected and analyzed using time-diary method, and the collected data were compared with the directly observed work time using GPS device. Background: There is no consistent conclusion which measurement method for work time is accurate. Most of the research results were collected from the environment other than rural area. Therefore, there is need to review reliability of the measurement methods for the agricultural work which are carried out for a long duration. Method: Top five crops (apple, pear, grape, sweet persimmon, and peach) were selected according to the number of farms and cultivation areas. We let fruit growers fill a work diary during one cropping period. A difference between the work-time that was recorded by the farmers and the direct observed worktime using GPS device were analyzed. Results: Average direct work time for the five fruit crops found to be 7.1% of the total work time. Conclusions and Applications: Reliability was secured by recording the daily work diary at each work stage while aware that direct observation is carried on. Data which are being collected by government institutions are obtained by the memories of the respondents. Therefore, it is needed to try the method adopted by this study in order to collect more accurate data.

• Journal of the Korean Society of Food Culture
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• v.34 no.6
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• pp.748-760
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• 2019

This study examined the difference of dietary intake behavior between groups of adults aged >20 years with or without dyslipidemia, and comparing the various types of dyslipidemia, based on the Seventh (2016) KNHANES. Men showed higher rate for triglyceridemia and LDL-cholesterolemia than women and subjects in the age group 50-59 years exhibited the highest incidence of triglyceridemia, total cholesterolemia and HDL-cholesterolemia. The ratio of obese people was higher in most types of dyslipidemias. The top five foods in each group of cereal & grain, meat, vegetable, and fruit were selected for analysis, based on the food frequency. The dyslipidemia group showed higher intake frequencies for mixed grains, cabbage kimchi and leafy vegetable than the no-dyslipidemia group, and the group with high blood total cholesterol for mixed grains, apple, and cutlassfish-croaker. Group with high blood triglyceride showed higher intake frequencies of rice and mackerel-mackerel pike than the other groups of high blood triglyceride. No food showed significant difference in the frequencies between groups of LDL-cholesterol. Group with high blood HDL-cholesterol showed higher intake frequency of bibim-fried rice, deep fried chicken, stir fried chicken, apple, tangerine, banana and butter-margarine. These results indicate that people with high levels of blood total cholesterol are more concerned with food having health benefits than those with abnormal levels of other blood lipid. More information on dietary benefits need to be provided to patients having high levels of triglyceride, LDL-cholesterol and HDL-cholesterol.

• Mycobiology
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• v.49 no.3
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• pp.249-257
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• 2021

Plants pollination are conducted through various pollinators such as wind, animals, and insects. Recently, the necessity for artificial pollination is drawing attention as the proportion of natural pollinators involved is decreasing over the years. Likewise, the trade in pollen for artificial pollination is also increasing worldwide. Through these imported pollens, many unknown microorganisms can flow from foreign countries. Among them, spores of various fungi present in the particles of pollen can be dispersed throughout the orchard. Therefore, in this study, the composition of fungal communities in imported pollen was revealed, and potential ecological characteristics of the fungi were investigated in four types of imported pollen. Top 10 operational taxonomic unit (OTU) of fungi were ranked among the following groups: Alternaria sp., Cladosporium sp., and Didymella glomerata which belong to many pathogenic species. Through FUNGuild analysis, the proportion of OTUs, which is assumed to be potentially plant pathogens, was higher than 50%, except for apple pollen in 2018. Based on this study of fungal structure, this information can suggest the direction of the pollen quarantine process and contribute to fungal biology in pollen