• Proceedings of the Korean Society of Precision Engineering Conference
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• 1991.04a
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• pp.150-157
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• 1991

As the system monitoring technology become required in order to imporve the system performance and the productivity, We've studied to the detection for the tool breakage using AE sensor that is able to detection of generated high frequency strss pulse at cutting. The method of spectrum analysis are used for analysis of AE signals in detection system. The experiments are carred out in a CNC lathe.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.10a
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• pp.159-159
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• 2003

Comet assay is a potential monitoring tool because DNA strand breakage may be produced by a wide range of agents. The comet assay, also called the single-cell gell electrophoresis (SCGE) assay, is rapid and sensitive method for the detection of DNA damage in cells. This study was performed for the identification of DNA damage in the cells from flounders and clams exposed to PAHs. As a control experiments, flounder and clam cells were exposed to $H_2O$$_2$. The cells exposed to $H_2O$$_2$ were displayed a typical nuclei movement DNA damage of cells were significantly increased when the isolated cells from the blood of flounders and the tissue of clams were in vitro exposed to the different concentrations (5, 10, 50, 100 ppb) of five kinds of PAHs (benzo[a]pyrene, pyrene, fluoranthene, anthrancene, and phenanthrene). For the in vivo test, flounders and clams were exposed to the different concentrations of BaP for 4 days. The results showed that DNA strand breakage was effected by the concentration of BaP and the duration of exposure. In high concentration of BaP, the mean tail lengths of nuclei was longer than it In low concentration, while the mean size of head DNA decreased. In this research, both in vitro and in vivo genotoxicity of PAHs could be biomonitored by the comet assay. Especially, clams and flounders seem to be useful as materials for monitoring genotoxic damage by comet assay.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.16 no.6
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• pp.41-46
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• 2017

Productivity improvement and cost reduction in the aircraft industry have become major industrial objectives, and improving productivity by reducing machining time has become a key focus. When numerical cutting code is created by CAM software, such as CATIA or UG-NX, it is impossible to control machining feed speed using cutting force changes depending on the machining tool path. However, machining an aircraft engine part from difficult material, such as Inconel 718, takes a long time, and tool chipping or breakage often occurs from forcing the machining path too quickly. This study investigated and verified the reliability of the AdvantEdge production module (PM)using cutting power tests. In particular, diffuser and diffuser case parts were considered, comparing cutting power and machining time using AdvantEdge PM and CATIA.

• Ocean and Polar Research
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• v.33 no.2
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• pp.127-133
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• 2011

The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide ($H_2O_2$); strong correlation between $H_2O_2$ concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1, 10, 50, 100 ${\mu}g/L$) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.