• 제목/요약/키워드: tolerance to sulfate

검색결과 19건 처리시간 0.024초

Characterization of Sulfur Oxidation by an Autotrophic Sulfur Oxidizer, Thiobacillus sp. ASWW-2

  • Lee Eun Yaung;Cho Kyung-Suk;Ryu Hee Wook
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제5권1호
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    • pp.48-52
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    • 2000
  • An autotrophic sulfur oxidizer, Thiobacillus sp. ASWW-2, was isolated from activated sludge, and its sulfur oxidation activity was characterized. Thiobacillus sp. ASWW-2 could oxidize elemental sulfur on the broad range from pH 2 to 8. When 5-50 g/L of elemental sulfur was supplemented as a substrate, the growth and sulfur oxidation activity of Thiobacillus sp. ASWW-2 was not inhibited. The specific sulfur oxidation rate of strain ASWW-2 decreased gradually until sulfate was accumulated in medium up to 10 g/L. In the range of sulfate concentration from 10 g/L to 50 g/L, the sulfur oxidation rate could keep over $2.0g-S/g-DCW{\cdot}d$. It indicated that Thiobacillus sp. ASWW-2 has tolerance to high concentration of sulfate.

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주요 복강수술 환자에서 황산 마그네슘의 아편유사제 내성 감소에 대한 효과 (Magnesium Sulfate Attenuate Opioid Tolerance in Patients undergoing Major Abdominal Surgery)

  • 장미순;손용;이철;이주환;박정현;이명종
    • The Korean Journal of Pain
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    • 제22권1호
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    • pp.58-64
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    • 2009
  • Background: Magnesium is a noncompetitive antagonist of the N-methyl-D aspartate (NMDA) receptor. Magnesium is thought to be involved in opioid tolerance by way of inhibiting calcium entry into cells. Methods: The patients were randomly assigned to three groups according to the anesthetic regimens: Group M received magnesium sulfate and Group C received saline intravenously under remifentanil-based anesthesia. Group S received saline intravenously under sevoflurane based anesthesia in place of remifentanil. The patients in the group M received 25% magnesium sulfate 50 mg/kg in 100 ml of saline, and those patients in groups C and S received an equal volume of saline before induction of anesthesia; this was followed by 10 mg/kg/h infusion of either magnesium sulfate (group M) or an equal volume of saline (groups C and S) until the end of surgery. Pain was assessed on a visual analog scale at 1, 6, 12, 24, and 36 hours after the operation. The time to the first postoperative analgesic requirement and the cumulative analgesic consumption were evaluated in the three groups. Results: The visual analog scales for pain and the cumulative analgesic consumption were significantly greater in group C than in other groups. The time to first postoperative analgesic requirement was significantly shorter in group C than that in the other groups. There were no differences between group M and S for side effects. Conclusions: A relatively high dose and continuous remifentanil infusion is associated with clinically relevant evidence of acute opioid tolerance. NMDA-receptor antagonist, magnesium sulfate as an adjuvant analgesic prevents opioid tolerance in patients who are undergoing major abdominal surgery under high dose and continuous remifentanil infusion-based anesthesia.

양식 어류에 있어서 황산동(copper sulfate pentahydrate)의 안전성과 살균효과에 대한 연구 (Antibacterial efficacy and safety of copper sulfate pentahydrate to cultured fish)

  • 허강준
    • 대한수의학회지
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    • 제37권1호
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    • pp.203-212
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    • 1997
  • Antibacterial efficacy and safety of copper sulfate pentahydrate (5% solution) was evaluated in laboratory and field studies for disinfection of some fish bacterial pathogens in cultured fish, flounder(Paralichthys olivaceus), mirror carp(Cyprinus carpio), gold fish(Carrassius auratus) and eel(Anguilla japonica). Dipping of fishes for acute toxicity was performed for a period of 48h, and the TLm value(median tolerance limit) were 4.0ppm in mirror carp and flounder, 4.4ppm in rainbow trout, and 10.0ppm in gold fish. After exposure below than TLm value for 1 month, no side effects and histopathological changes were observed. The test drug were shown high antibacterial activities against most bacterial pathogens by exposure in the laboratory and field trials. However, Aeromonas salmonicida showed a resistance to the dosage. We can prevent outbreaks of bacterial diseases of fish by the dosage of 1ppm for 6hr and the test drug is thought to be used effectively and widely as a disinfectant against most bacterial fish pathogens.

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Screening and Characterization of Pro biotic Lactic Acid Bacteria Isolated from Korean Fermented Foods

  • Lim, Sung-Mee;Im, Dong-Soon
    • Journal of Microbiology and Biotechnology
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    • 제19권2호
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    • pp.178-186
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    • 2009
  • To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

Mercury Resistance and Removal Mechanisms of Pseudomonas sp. Isolated Mercury-contaminated Site in Taiwan

  • Luo, Kai-Hong;Chen, Ssu-Ching;Liao, Hung-Yu
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제21권5호
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    • pp.16-24
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    • 2016
  • A new strain of Pseudomonas sp. was isolated from mercury (Hg)-contaminated sites in Taiwan. This bacterium removed more than 80% of Hg present in the culture medium at 12 h incubation and was chosen for further analysis of the molecular mechanisms of Hg tolerance/removal abilities in this Pseudomonas sp. We used RNA-seq, one of the next-generation sequencing methods, to investigate the transcriptomic responses of the Pseudomonas sp. exposed to 60 mg/L of Hg2+. We de novo assembled 4,963 contigs, of which 10,533 up-regulated genes and 5,451 down-regulated genes were found to be regulated by Hg. The 40 genes most altered in expression levels were associated with tolerance to Hg stress and metabolism. Functional analysis showed that some Hg-tolerant genes were related to the mer operon, sulfate uptake and assimilation, the enzymatic antioxidant system, the HSP gene family, chaperones, and metal transporters. The transcriptome were analyzed further with Gene Ontology (GO) and Cluster of Orthologous Groups (COGs) of proteins and showed diverse biological functions and metabolic pathways under Hg stress.

Glutathione Reductase from Oryza sativa Increases Acquired Tolerance to Abiotic Stresses in a Genetically Modified Saccharomyces cerevisiae Strain

  • Kim, Il-Sup;Kim, Young-Saeng;Yoon, Ho-Sung
    • Journal of Microbiology and Biotechnology
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    • 제22권11호
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    • pp.1557-1567
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    • 2012
  • Glutathione reductase (GR, E.C. 1.6.4.2) is an important enzyme that reduces glutathione disulfide (GSSG) to a sulfydryl form (GSH) in the presence of an NADPH-dependent system. This is a critical antioxidant mechanism. Owing to the significance of GR, this enzyme has been examined in a number of animals, plants, and microbes. We performed a study to evaluate the molecular properties of GR (OsGR) from rice (Oryza sativa). To determine whether heterologous expression of OsGR can reduce the deleterious effects of unfavorable abiotic conditions, we constructed a transgenic Saccharomyces cerevisiae strain expressing the GR gene cloned into the yeast expression vector p426GPD. OsGR expression was confirmed by a semiquantitative reverse transcriptase polymerase chain reaction (semiquantitative RT-PCR) assay, Western-blotting, and a test for enzyme activity. OsGR expression increased the ability of the yeast cells to adapt and recover from $H_2O_2$-induced oxidative stress and various stimuli including heat shock and exposure to menadione, heavy metals (iron, zinc, copper, and cadmium), sodium dodecyl sulfate (SDS), ethanol, and sulfuric acid. However, augmented OsGR expression did not affect the yeast fermentation capacity owing to reduction of OsGR by multiple factors produced during the fermentation process. These results suggest that ectopic OsGR expression conferred acquired tolerance by improving cellular homeostasis and resistance against different stresses in the genetically modified yeast strain, but did not affect fermentation ability.

규산 및 칼리 시비에 따른 벤트그래스 생육 및 내답압성 반응 (Growth and Wear Tolerance of Creeping Bentgrass as Influenced by Silica and Potassium Fertilization)

  • 김용선;김기선
    • 아시안잔디학회지
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    • 제26권2호
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    • pp.116-122
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    • 2012
  • 본 연구는 규산과 칼리 시용이 골프장 그린용 크리핑 벤트그래스 잔디의 생육과 품질 그리고 내답압성에 미치는 영향을 알아보기 위해 실시되었다. 먼저 규산과 칼리 시용에 따른 잔디반응을 살펴보기 위해서 필드조건에서 잔디품질, 뿌리길이, 잔디밀도와 건물중을 평가하였다. 다음으로 답압빈도에 따른 크리핑 벤트그래스의 잔디생육(뿌리길이, 잔디밀도, 건물중)과 잔디품질 및 토양경도를 조사하였다. 마지막으로 30일간의 답압처리조건에서 규산과 칼리의 내답압성 시비효과를 평가하였다. 규산 및 칼리 처리에 따른 잔디뿌리생육에 있어서 규산을 증가함에 따라 뿌리길이가 증가하였다. 크리핑 벤트그래스의 답압빈도에 따른 잔디품질은 1일 2회 처리구가 가장 우수하였으며, 7회 처리구는 무답압 처리구보다 우수하였다. 그러나 15회 이상 답압 처리구는 잔디품질이 월등히 저하하였다. 30일간의 답압처리시 규산 및 황산칼리의 시비효과는 잔디품질 향상 및 뿌리생육 증진효과가 있었다. 규산과 칼리를 처리하였을 때 잔디품질이 대조구보다 각각 6.38%, 10.25% 상승하였으며 규산과 칼리를 혼용 살포하였을 때 가장 잔디품질이 좋았다. 잔디 뿌리길이는 규산을 살포하였을 때 가장 길었으며 대조구보다 11.4%가 길었다. 따라서 답압이 예상되는 골프장 그린용 벤트그래스에서 잔디의 내답압성 증진을 위해서는 규산과 칼리의 시비가 효과적이었다.

저서성 해산 요각류 harpacticoid Tigriopus japonicus 유생을 이용한 해양생태독성평가 (Marine Ecotoxicological Assessment Using the Nauplius of Marine Harpacticoid Copepod Tigriopus japonicus)

  • 윤성진;박경수;오정환;박승윤
    • 한국해양환경ㆍ에너지학회지
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    • 제9권3호
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    • pp.160-167
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    • 2006
  • 저서성 요각류 Tigriopus japonicus 는 한국연안에 널리 분포하며, 영양단계에서 1차 소비자로서 중요한 역할을 담당한다. 본 연구에서는 해양생태독성평가를 위한 표준 실험생물로서 가능성을 구명하기 위하여 T. japonicus 유생($100{\sim}200{\mu}m$)을 이용하여 염분 및 수소이온농도(pH) 내성실험과 2종의 표준독성물질(황산구리, 카드뮴) 및 3종의 산업폐수오니(피혁, 염색, 염료공장폐수오니)에 대한 48시간 사망률 실험을 실시하였다. T. japonicus 유생은 $5.0{\sim}35.0psu$ 염분과 pH $6.3{\sim}8.2$ 범위에서 90% 이상 생존하여 염분 및 pH에 대한 강한 내성을 보였다. 또한 표준독성물질에 대한 48시간 $LC_{50}$은 황산구리의 경우 $3.6{\pm}0.7ppm$, 카드뮴은 $1.7{\pm}0.8ppm$ 이었으며, 각각의 반복구간의 사망률 편차는 10% 이하였다. 황산구리에 대한 T. japonicus 유생의 독성반응은 요각류를 포함한 소형 갑각류에 비해 낮은 것으로 나타났다. 그러나 카드뮴에 대한 T. japonicus 유생의 독성반응은 요각류 T. japonicus, Paracalanus parvus 및 윤충류 Brachinonus plicatilis 성체 보다 훨씬 민감하였다. 산업폐수오니의 경우, 염료오니 추출액을 제외한 피혁과 염색오니 추출액은 농도-반응 사이의 선형관계가 뚜렷하였다. 산업폐수오니 추출물에 대한 T. japonicus의 48시간 $LC_{50}$은 피혁 추출물의 경우, $31.1{\pm}1.1%$ 이었으며, 염색 추출물은 $54.4{\pm}15.1%$로 산출되었다. 상기의 실험결과들에 의하면, 요각류 T. japonicus 유생을 이용한 생물검정법은 산업폐수나 화학물질에 대한 해양생태독성평가 시 유용한 평가 수단인 것으로 판단된다. 광범위한 염분 및 pH에 대한 내성, 농도-반응(사망률) 사이의 뚜렷한 선형관계는 본 종이 표준시험종으로써 충분한 가치가 있음을 입증하였다.

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매립지 악취가 주변 대기질에 미치는 영향 예측 - 청주청원 광역매립지 사례연구 - (Prediction of Malodorous Landfill Substances Effect on Ambient Air Quality - A Case Study on Cheongju·Cheongwon Metropolitan Landfill -)

  • 이상우
    • 환경영향평가
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    • 제21권5호
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    • pp.695-705
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    • 2012
  • The purpose of this study is to investigate concentration level and characteristics of malodour substances generated from landfill site in C city. Also, it is tried to predict distribution of concentration level using ISCST3 model around landfill site. From the results, it can be confirmed that twelfth-class malodour substances such as ammonia, methyl mercaptan, hydrogen sulfide, dimethyl sulfate, dimethyl disulfate, toluene, acetaldehyde, styrene, propionaldehyde, butylaldehyde, n-Valeraldehyde, xylene were generated from landfill site. The levels of the malodour substances were lower than that of permeable concentration regulated by odor control law in Korea. However, the concentration of malodour substances including methyl mercaptan, hydrogen sulfide, acetaldehyde, and propionaldehyde exceeded threshold limit value(TLV). It was seemed that these substances caused the problem of offensive odor around circumstance of landfill. The concentration of malodour substances was higher in slant than in upper part of landfill. The concentrations of malodour substances measured at night time were shown higher level than those at night time because atmospheric condition was stable at night time. It showed that the concentration of malodour substances were higher in spring. The results of atmospheric diffusion model predicted that tolerance limit level of hydrogen sulfide and methyl mercaptan was detected within nearly 5km from the boundary of landfill.

Expression and Biochemical Characterization of Cold-Adapted Lipases from Antarctic Bacillus pumilus Strains

  • Litantra, Ribka;Lobionda, Stefani;Yim, Joung Han;Kim, Hyung Kwoun
    • Journal of Microbiology and Biotechnology
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    • 제23권9호
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    • pp.1221-1228
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    • 2013
  • Two lipase genes (bpl1 and bpl3) from Antarctic Bacillus pumilus strains were expressed in Bacillus subtilis. Both recombinant lipases BPL1 and BPL2 were secreted to the culture medium and their activities reached 3.5 U/ml and 5.0 U/ml, respectively. Their molecular masses apparent using SDS-PAGE were 23 kDa for BPL1 and 19 kDa for BPL3. Both lipases were purified to homogeneity using ammonium sulfate precipitation and HiTrap SP FF column and Superose 12 column chromatographies. The final specific activities were estimated to be 328 U/mg for BPL1 and 310 U/mg for BPL3. Both lipases displayed an optimum temperature of $35^{\circ}C$, similar to other mesophilic enzymes. However, they maintained as much as 70% and 80% of the maximum activities at $10^{\circ}C$. Accordingly, their calculated activation energy at a temperature range of $10-35^{\circ}C$ was 5.32 kcal/mol for BPL1 and 4.26 kcal/mol for BPL3, typical of cold-adapted enzymes. The optimum pH of BPL1 and BPL3 was 8.5 and 8.0, respectively, and they were quite stable at pH 7.0-11.0, showing their strong alkaline tolerance. Both lipases had a preference toward medium chain length ($C_6-C_{10}$) fatty acid substrates. These results indicate the potential for the two Antarctic B. pumilus lipases as catalysts in bioorganic synthesis, food, and detergent industries.