• Journal of the Korean Society of Tobacco Science
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• v.4 no.1
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• pp.43-49
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• 1982

This experiment was carried out to study the influence of temperature on the growth rate and nutrient uptake of tobacco seedlings grown on neutralized charcoal bed during temporary transplanting. The highest growth rate was obtained at tile temperature of 26-22-$l8^{\circ}C$, probably due to fast establishment of roots in the soil. Seedling growth was better in neutralized charcoal bed than in chaffy charcoal bed at each temperature. Days required for fresh weight to reach 20g/10p1ants were shortened by 9~12days as the temperature increased from 18-14-$l0^{\circ}C$ to 26-22-$l8^{\circ}C$. Compared with chaffy charcoal bed, days required for fresh weight to reach 20g/10 plants with neutralized charcoal bed were shortened by about 5 days, 4 days, and 2days at 18-14-$l0^{\circ}C$, 22-18-$l4^{\circ}C$, and 26-22-$l8^{\circ}C$, respectively. Three macronutrient (N, $P_2O_5,\;K_2O$) contents in seedlings increased with increase of temperature. At each temperature, N and $K_2O$ contents in seedlings of neutralized charcoal bed was higher than those of chaffy charcoal bed, whereas that of $P_2O_5$ was low conversely. Nitrate nitrogen from nitric acid that was used to neutralize chaffy charcoal was maintained to tile end of seedling period, providing the source of nitrate nitrogen for the better seedlings.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.152-160
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• 1993

Using a flue- cured tobacco variety, KF 109, effect of growth regulators(fatty alcohol and C- MH) on the change of protein, DNA, and RNA were investigated. Generally, inhibition of DNA synthesis was observed soon after become notably reduced when checked on 14 days after the treatment. Fatty alcohol treatment appeared to alleviate the inhibition of DNA synthesis caused by the C - MH treatment. It was also observed that in the tips DNA content increased slightly at the early stage after the C - MH treatment but evident reduction of it was resulted from 7th day after the treatment. RNA content in cutters and tips was increased initially but variable transcription inhibitory activities - not so obvious as was observed in DNA synthesis - according to leaf positions were shown thereafter. Ripening of leaves probably due to senescence was advanced by the treatment of the growth regulators. DNA content in root was relatively higher in plants treated with the growth regulators while it was clearly decreased in stalk, However, RNA contents in tissue of stalk and root was not different with that of foliage. Increase of protein content in foliage as well as in stalk was evident 14 days after dual treatment of fatty alcohol and C - MH.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.1
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• pp.84-86
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• 1982

The experiment was conducted with three varieties (Hicks, Burley 21, and Sohyang) and cultivation type (Improved mulching, general mulching, and non mulching) of NC 2326 to model to curve of tabacco growth against time. The basic growth data were obtained by harvest method at intervals of ten days from transplanting at 7-8 times and analyzed by polynomial regression, orthogonal polynomial, and logarithmic transformation. It is shown that the C model of growth curve: T = A +$\sqrt{(1.4 AK + K)}$2K provides an excellent fit.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.1
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• pp.33-41
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• 1992

These studies were conducted to investigate effects of phytohormone and activated carbon on the growth and rooting of teratoma shoots induced from Nicotiana tabacum cv. NC2326 transformed by Aerobacterium tumefaciens C58. GA was effective for shoot elongation and reduction of multiple shoots from teratoma shoot, however, leaves of teratoma shoot cultured on the medium with GA were pointed. ABA was also effective in promoting shoot elongation, but was not for reduction of multiple shoots. Teratoma shoot cultured on the medium with 1 n activated carbon promoted shoot elongation and inhibited the number of shoots differentiated, but was grown as abnormal shoot. Addition of 1% activated carbon and 0.5mg/l BA to culture media was effective for shoot elongation and reduction of multiple shoot and for formation of round leaves as normal leaves. Though these shoots were inoculated on the rooting medium, they could not from roots but formed multiple shoots. Boric acid, myo-inositol and sucrose were also ineffective on the rooting of teratoma shoots.

• Mycobiology
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• v.29 no.1
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• pp.48-53
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• 2001

Plants have the ability to acquire an enhanced level of resistance to pathogen attack after being exposed to specific biotic stimuli. To obtain plant growth-promoting rhizobacteria inducing resistance against cucumber anthracnose by Colletotrichum orbiculare, more than 800 strains of rhizobacteria were screened in the greenhouse. Among these strains, Bacillus amyloliquefaciens solate EXTN-1 showed significant disease control efficacy on the plants. Induction of pathogenesis-related(PR-la) gene expression by EXTN-1 was assessed using tobacco plants transformed with PR-1a::$\beta$-glucuronidase(GUS) construct. GUS activities of tobacco treated with EXTN-1 and salicylic acid-treated transgenic tobacco were significantly higher than those of tobacco plants with other treatments. Gene expression analyses indicated that EXTN-1 induces the accumulation of defense-related genes of tobacco. The results showed that some defense genes are expressed by the treatment with EXTN-1 suggesting the similar resistance mechanism by salicylic acid.

• Korean Journal of Health Education and Promotion
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• v.7 no.2
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• pp.71-77
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• 1990

Streptococcus mutans 10449 was cultured in sucrose-containing BHI broth with ethyl alcohol of different concentration from 1% to 18%, The pH of culture media was from pH 7.00 to pH 5.00. Tobacco smoke and tobacco extract were also used. Ethyl acohol began to inhibit sucrose fermentation by S. mutans at 2% and completely inhibited it between 9% and 18%. The lower the pH of media was, the stronger the inhibition of ethyl alcohol became. 9% Ethyl alcohol completely inhibited sucrose fermentation by S. mutans below pH 5.50, Inhibition by tobacco extract was obvious, but it did not inhibit the growth of S. mutans also. Therefore, the increase of caries activity in drinkers and smokers could be the result of indirect effect of alcohol and tobacco by oral ecology, behavior, or systematic course, rather than the result of direct effect of alcohol and tobacco to plaque bacteria and their metabolism.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.2
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• pp.3-7
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• 1983

Three levels of nitrogen, phosphate and potassium were applied to tobacco for the establishment of the optimum rate of the fertilizers for yield and quality. 1 . The rate increment of nitrogen and potassium increased tobacco growth, but phosphate were non - significant between rate. 2. The contents of total-nitrogen and total-alkaloid in the leaf were increased, with the increase of nitrogen rate while the effect of phosphate and potassium were negligible. 3. The yield and quality of the tobacco were increased with the increasing rate of nitrogen and potassium fertilizer but phosphate should little difference.

• Journal of the Korean Society of Tobacco Science
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• v.21 no.2
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• pp.152-159
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• 1999

A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

• Journal of the Korean Society of Tobacco Science
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• v.7 no.1
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• pp.3-6
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• 1985

The typical dark brown stripe symptom of bacterial wilt disease was observed in the cuttings of tobacco stem treated with the culture filtrate of virulent Pseudomonas solanaceamm. And the tobacco callus create.4 with that culture filtrate showed deterioration of the callus 2 days after the treatment. On the contrary, the cuttings and the callus treated with the culture filtrate of the avirulent bacteria expressed no typical symptom and vigorous growth respectively. Therefore it was suggested that certain toxin which might be produced by the virulent bacteria could break down tobacco cells.

• Journal of the Korean Society of Tobacco Science
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• v.8 no.1
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• pp.57-67
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• 1986

This study was conducted to find out the effects of chlorine on the yield, chemical constituents and physical properties of Burley leaf tobacco. The results obtained are as follows. 1. There was no significant differences in growth and yield among chlorine application levels. 2. When chlorine being applied 4kg/10a or more, the cured leaf showed higher chlorine content and lower leaf quality remarkably. It was estimated that the critical application of chlorine was 3kg/10a. 3. Total nitrogen and alkaloid contents of cured leaf were increased by chlorine application, while the phosphorus, calcium and magnesium were not affected. 4. The reflectance color of red and yellow were decreased by cvhlorine application in cured leaf of field grown tobacco.