• 한국작물학회지
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• 제42권5호
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• pp.632-639
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• 1997

본 실험은 담배의 최대생장 후반기에 수분제한처리를 하여, 한발이 엽위에 따라 생장과 발달에 미치는 영향을 분석하고, 엽영에 따른 엽내 수분상태가 기공컨덕턴스, 잎수분포텐셜과 토양수분관계에 영향을 미치는 생리적 반응을 구명하고져 실시하였다. 담배의 최대생장기 한발영향은 지상부의 제형질을 감소시켰고, 엽위에 따른 생장반응은 중ㆍ하위엽에서 작았고 상위엽에서 컸다. 처리 5일째에 잎의 상대수분함량은 토양수분함량이 4.3%로 감소될 때 상위엽 74%, 중위엽 64%, 하위엽 59%로 담배잎의 위주점은 상대수분함량이 약 75%이었다. 한발에 따른 잎수분포텐셜은 대조구의 -0.58 MPa에서 처리구의 처리5일째에 -1.20 MPa로 떨어졌고, 대조구와 비교하여 약 20%의 수분포텐셜차이가 위주점이 되었다. 기공컨덕턴스는 중ㆍ상위엽에서 12 mol /$\textrm{m}^2 sec^{-1}$에서 0.8 mol /$\textrm{m}^2 sec^{-1}$로 떨어져, 한발처리에 따른 담배의 최대생장후반기의 엽위에 따른 생장반응은 중ㆍ상위엽까지 영향을 미쳤다.

• 한국연초학회지
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• 제29권2호
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• pp.59-65
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• 2007

The study was carried out to investigate water activity, physicochemical properties and color degree of burley tobacco stored in shelf with different moisture content for 56 days. The study was consist of two experimental designs, that is, moisture content in burley tobacco was kept to 13 to 15 %, 17 to 19 %, and 21 to 23 % under ambient temperature of $20^{\circ}C$ with relative humidity of 24 %, and another group of in burley tobacco was only kept in ambient temperature of $3^{\circ}C$ with relative humidity of 50 % for 56 days with same water content of former treatment. Normal leaves were kept for 56 days under 21 to 23% of moisture content with increased water activity and TSNA(tobacco-specific nitrosamine content. It was considered that favorable leaves conditions are due to favorable microbial growth resulting in increased TSNA content. The total nitrogen content has not changed in two experimental groups for 56 days, otherwise the nitrate form nitrogen and total alkaloid contents were only increased by 21 to 23 % of water content. Degree of pH change was slightly decreased in both storage and water treatment. It was more decreased in 21 to 23% of moisture content than that of other moisture content. In stored leaf tobacco, higher moisture content caused lower brightness, yellow degree, and carbonization quotient.

• 식물조직배양학회지
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• 제25권1호
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• pp.57-61
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• 1998

본 실험은 질소원처리가 식물의 생육 및 질소대사에 미치는 영향을 규명하고자 조직배양을 실시하였으며 공시재료로는 담배 'NC 2326'와 대두 '단엽콩'을 사용하였다. 배지내 질소원의 종류와 그 비율을 달리했을 때 callus의 생육, 전 질소, 단백질 함량 그리고 nitrate reductase와 glutamine synthase의 활성 변화를 조사하였다. 단엽콩의 조직배양시 callus증식은 2:1처리구와 암모늄태 질소만 공급한 배지에서 높았던 반면 질산태만 공급한 경우 쉐는 callus증식이 저조하였다. 한편 담배는 질산태와 암모늄태 질소를 2:1로 혼용한 구가 callus증식이 좋았고, 암모늄태 질소단용구에서 저조하였으며, 질산태 질소단용구의 callus생육이 양호하였다. Callus내 전질소함량은 담배의 경우 배지내 질산태 질소가 많을수록 증가되어 질산태 질소단용구에서 가장 많았으며 암모늄태 질소단용구에서 가장 적었다. 한편 단엽콩은 이와 반대의 경향으로 암모늄태 질소단용구에서 가장 많았으며 질산태 질소단용구에서 가장 적었다. Callus의 nitrate reductase의 활성을 보면 담배는 질산태 질소단용구에서 활성이 높았으며 암모늄태 질소단용구에서 활성이 낮은 반면 단엽콩은 2:1 처리구에서 활성이 높았고 생육이 부진했던 질산태 질소단용구에서 현저하게 낮았다. Glutamine synthetase의 활성은 단엽콩, 담배 모두 질산태 질소단용구에서 활성이 높았으며 암모늄태 질소단용구에서 활성이 낮았다.

• 한국연초학회지
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• 제10권1호
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• pp.11-19
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• 1988

This study was conducted to relate among growth characteristics, quality, and chemical components for flue-cored tobacco. The results are summarized as follows : 1. Starch content in harvested green leaf was correlated negatively with leaf length, leaf width, leaf area, and leaf weight of harvested green leaf, respectively, while positively with total sugar content in cured leaf, and the ratio of leaf length to leaf width of harvested green leaf. 2. Organic matter and total nitrogen contents in the soil were correlated positively with nicotine content in cured leaf, respectively, and total nitrogen in the soil negatively with total sugar content in cured leaf. 3, Amount of fertilizer, application date of MH, priod of harvesting, and yield were correlated positively with nicotine content in cured leaf, respectively , while negatively with total sugar content. Application amount of MH was correlated positively with total sugar content, but negatively with nicotine content in cured leaf. Also amount of compost was correlated negatively with nicotine content in cured leaf. 4. The ratio of total sugar to nicotine (TS/N) per plant was correlated positively with price (Won/kg) and specific leaf area, but negatively with leaf length. leaf width, leaf area, harvested leaves, and leaf weight in cured leaves, respectively. Yield was correlated negatively with TS/N per plant. 5. TS/N of the best quality tobacco per plant was 12.0. Those of the best quality tobacco in each stalk position were 42.1 for first~third leaf, 28.4 for 4th~6th leaf , 23.7 for 7th~9th leaf, 7.7 for l0th~12th leaf , and 7.8 for over 13th leaf from bottom, respectively. 6, When TS/N was 12.0, optimum values of growth characteristics per plant were 100.5 $\pm$ 10.3g for leaf dry weight, 755.1$\pm$53.2cm for leaf length, 294.4$\pm$25.1cm for leaf width, 8, 892$\pm$111cm2 for leaf area, 16.0$\pm$0.6 leaves for harvested leaves, and 7.32$\pm$0.44mg/cm2 for specific leaf area, respectively. 7. When TS/N was 12.0, optimum values of chemical components per plant were 1.92$\pm$0.28% for nicotine content, and 20.4$\pm$ 1.4 % for total sugar content, and that of yield was 238.3$\pm$ 9.8kg/10a.

• 한국연초학회지
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• 제20권2호
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• pp.153-159
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• 1998

The vein necrotic strain of Potato Virus Y (PVY - VN) and black shank (Phyto-phthora parasitica var. nicotianae) are the two major diseases causing severe damages especially in burley tobacco (N. tabacum L.) area in Korea. A new tobacco variety, KB 111, resistant to PVY and black shank disease, was developed by Korea Ginseng & Tobacco Research Institute in 1997. It is a male sterile(MS) F$_1$ hybrid of the cross between MS TC 613 and KB 108. KB 111 was compared to Burley 21 on the agronomic characteristics and disease resistances in performance tests: It possessed upright growth habit and flowered two days later than Burley 21. It was resistant to both PVY and black shank and yielded about 3% more cured leaf than Burley 21, but other characteristics are very simiar to those of Burley 21. The chemical composition and physical properties of the cured leaf of KB 111 were as much acceptable as those of Burley 21 while it produced average yield of good quality leaf and appeared to resistant to PVY and black shank disease on regional farm test in 1998.

• 한국연초학회지
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• 제20권2호
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• pp.198-204
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• 1998

This study was conducted to evaluate the effect of far IR radiation for the elimination of sidestream smoke components in a closed room. The measurements covered particle sizes of 13.8-542.5 nm, particle concentration, TSP, UVPM, FPM, solanesol, and the following gases and vapor components of smoke: carbon dioxide, carbon monoxide, nicotine, and 3-ethenyl-pyridine. Also, the changes of smoke odor strength by far IR radiation were tested by using the electronic nose system. There was no difference between control and far IR radiation in changes of the concentration of $CO_2$ and CO. The concentrations of TSP, UVPM, FPM, solanesol, nicotine, and 3-ethenylpyridine were reduced by far IR radiation. The growth and diminishing rate of RSP diameter was accelerated by far IR radiation compared with control. There was a little difference of smoke odor change with far IR radiation by electronic nose system analysis. Our results indicated that the use of far IR radiation had a little effect on changes of solid, vapor, and odor of smoke, but it had no effect on gaseous components.

• 한국잡초학회지
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• 제18권3호
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• pp.237-245
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• 1998

Bacillus subtilis protoporphyrinogen oxidase 유전자를 Agrobacterium을 매개체로 이용하여 전이 시킨 후 cauliflower mosaic virus 35S promoter하에서 발현케 한 형질전환 담배를 유기하였다. 이러한 형질전환 담배의 diphenyl ether계 제초제 oxfluorfen에 대한 생리적 반응과 여러 환경 조건에서의 생장 반응을 재배종 담배와 비교하였다. Oxyfluorfen의 처리에 의해 나타나는 세포내 구성물질의 누출과 지질과산화작용은 재배종 담배에서보다 형질전환 담배에서 더 작게 이루어졌다. 형질전환 담배의 생장을 여러 온도, 광도 및 수분 조건에서 조사한 결과, 저광도와 포화수분 조건에서의 생장이 재배종 담배에 비해 다소 저하되는 현상이 나타났을 뿐 기타 조건에서의 생장은 재배종 담배의 생장과 거의 동일하였다. 따라서 B. subtilis protoporphyrinogen oxidase 유전자를 전이시켜 cauliflower mosaic virus 35S promoter하에서 발현케 한 형질전환 담배는 oxyfluorfen에 대해 비교적 높은 저항성을 나타내지만 형질전환에 따른 생장 변화는 크게 일어나지 않음을 알 수 있었다. 한편 이러한 형질전환 담배의 oxyfluorfen에 대한 저항성 기작에 대해서도 논의하였다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.101.2-102
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• 2003

The ability of P. chlororaphis O6 to induce resistance to Erwinia carotovora subsp. carotovara SCCI and to promote growth in tobacco was demonstrated in microtiter assays on plants pre-inoculated at the root level with the bacteria before challenge with the leaf pathogen. To identify th bacterial determinants involved in induced systemic resistance and plant growth promotion, cell culture of O6 grown in King's medium B was fractionated with organic solvents and purified using various columns. in vivo and in vitro assays with samples from successive fractionation steps of the O6 supernatant led to the conclusion that antibacterial compounds were observed in aqueous layer, and to the isolation of fractions containing metabolites that retained most of the resistance-inducing activity (70：30, methanol：water) and the plant growth promotion (80：20 and 90：10, methanol：water) after ODS column chromatography. Although these molecules remain to be purified further and structurally characterized, its isolation is an addition to the range of determinants from plant growth-promoting rhizobacteria known to stimulate plant defence.

• Journal of Ginseng Research
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• 제6권1호
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• pp.11-16
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• 1982

New shoot growth of Panax ginseng root was investigated comparing with burley and soybean from l0$^{\circ}C$ to 30$^{\circ}C$ under dark. Shoot growth ceased by 12days at 30$^{\circ}C$ and optimum temperature appeared to be 15$^{\circ}C$/20$^{\circ}C$ (15hrs/9 hrs) , and 15$^{\circ}C$/15$^{\circ}C$ for ginseng. Shoot growth seems to be Poor below l0$^{\circ}C$. Temperature for maximum growth 20$^{\circ}C$/20$^{\circ}C$ for barley and 20$^{\circ}C$ /25$^{\circ}C$ for soybean. Barley did not germinate above 25$^{\circ}C$/25$^{\circ}C$, but grow better than soybean below 15$^{\circ}C$/25$^{\circ}C$. Fresh weight of 2 weeks suggesting cessation of water uptake at higher temporal use. Ginseng showed greater root ply s shoot of ginseng was linearly increased at 15$^{\circ}C$ but did not increased at 25$^{\circ}C$ after occurence of die-back of new shoot or root rot above 25$^{\circ}C$.

• Journal of Ginseng Research
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• 제8권1호
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• pp.65-74
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• 1984

This experiment was carried out to study the effects of light intensity and soil water regimes on the growth of ginseng seedling. The results were as follows: 1. The maximum light intensity and optimum temperature in 1,le photosynthesis of ginseng seedling were 10,000 lux and 23 $^{\circ}C$. Respiration rate was increased at high temperature. 2. Air and soil temperature under the shading were increased as the increase of light intensity but soil water contents were decreased as the increase of light intensity, whereas air and soil temperature were decreased as the increase of precipitation under the shade b5: soil water contents were increased as the increase of precipitation under the shade. 3. The higher the transmittance of the shade, the greater the specific leaf weight (S.L.W.) and stomatal density. In contrast, however, the contents of total chlorophyll, chlorophyll a and b, and stomatal length was decreased. There was no any significant difference light intensity of the a/b ratio of chlorophyll. 4. The highest photosynthesis was occurred in ginseng leaves grown under the shade 5% L.T.R. and net photosynthesis rates increased with increasing soil water contents. 5. Optimum condition for usable seedling yield were 5% L.T.R. and 3.3% precipitation under the shade. Useless seedling increased with increasing precipitation under the shade.