• Journal of the Korean Society of Tobacco Science
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• v.30 no.2
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• pp.85-93
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• 2008

This report describes a set of seven informative single-nucleotide polymorphisms (SNPs) and one insertion-deletion (INDEL) distributed over 24 cultivars that can be used for tobacco (Nicotiana tabacum L.) cultivar identification. We analyzed 163,000 genomic DNA sequences downloaded from Tobacco Genome Initiative database and assembled 31,370 contigs and 60,000 singletons. Using relatively long contigs, we designed primer sets for PCR amplification. We amplified 61 loci from 24 cultivars and sequenced the PCR products. We found seven significant SNPs and one INDEL among the sequences and we classified the 24 cultivars into 10 groups. SNP frequency of tobacco, 1/8,380 bp, was very low in comparison with those of other plant species, between 1/46 bp and 1/336 bp. For exact identification of tobacco cultivars, many more SNP markers should be developed. This study is the first attempt to identify tobacco cultivars using SNP markers.

• Journal of the Korean Society of Tobacco Science
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• v.30 no.1
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• pp.1-7
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• 2008

Bacterial wilt caused by Ralstonia solanacearum has become a severe problem on tobacco in Korea. No effective single control measure is available at present time. One of the most potential way for controlling the bacterial wilt on tobacco is growing tobacco cultivars resistant to the bacterial wilt. In this study, optimal conditions for screening tobacco cultivars resistant to the bacterial wilt were examined to provide reproducible and efficient methods in growth chamber testing and field experiments for evaluating plant disease resistance. For this, already-known inoculation methods, inoculum densities, and incubation temperature, and plant growth stages at the time of inoculation were compared using tobacco cultivars resistant (Nicotiana tabacum cv, NC95), moderately resistant (N. tabacum cv. SPG70), and susceptible (N. tabacum BY4) to the bacterial disease. It was determined that root-dipping of tobacco seedlings at six true leaf stage into the bacterial suspension with inoculum level of $10^8$ colony-forming units (CFU)/ml for 20 min before transplanting was simple and most efficient in testing for resistance to the bacterial wilt of tobacco caused by R. solanacearum, for which disease incidences and severities were examined at 2 weeks of plant growth after inoculation at $20{\sim}25^{\circ}C$ in a growth chamber. These experimental conditions could discriminate one tobacco cultivar from the others by disease severity better than any other experimental conditions. In field testing, the optimum time for examining the disease occurrence was late June through early July. These results can be applied to establishing a technical manual for the screening of resistant tobacco cultivars against the bacterial wilt caused by R. solanacearum.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.1
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• pp.43-52
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• 1994

To investigate the factors of the improvement of cultivation methods according to the plant type, three Burley tobacco cultivars were done. Experiments on the variability of plant type and factors representing the productivity and quality of the cultivar were subsequently carried out in relation to different fertilizer levels. Results are summarized as below. Use of more fertilizer resulted in higher plant height and longer leaf length but smaller mean leaf inclination(MLI). The horizontal leaf area distribution became greater in parts distal to the stem and the vertical leaf area distribution became greater in lower half than in upper half as the amount of fertilizer applied was increased. Br. 49 was the largest but Br. 21 and NTN 77 were similar with respect to both CGR and NAR. The yield was greater in larger MLI cultivars. These three characteristics became greater when the tobacco were heavier in the cultivars of larger MLI and increased with higher amount of fertilizer. Harvest index became greater as the amount of fertilizer decreased but not significantly differ among the cultivars. Total nitrogen content and nitrate - form nitrogen content were greater, more amount of fertilizer, and larger MLI cultivar plots. Total nitrogen content was higher in upper leaves. The filling power of tobacco leaves decreased but combustibility of leaves showed not significant trend as fertilizer application level increased.

• Journal of the Korean Society of Tobacco Science
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• v.26 no.2 s.52
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• pp.73-78
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• 2004

This study was conducted to find out the relationship among agronomic characteristics including midrib ratio in Burley tobacco(N.tabacum L.) Four cultrivars, Burley 21, KB108, KB111 and KB301, were grown at Suwon Experimental Station in 2001. Several agronomic characteristics of these cultivars were measured for the analysis of correlations among agronomic characteristics. Midrib ratio of KB301 was somewhat higher than those of the other cultivars, but there is no significant differences among cultivars at $5\%$ probability level. Among four stalk positions, midrib ratio of cutters was the highest. Midrib ratio was correlated positively with length of leaf at $1\%$ level and negatively with weight of lamina at $5\%$ level, respectively.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.2
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• pp.94-99
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• 2006

AFLP(Amplified Fragment Length Polymorphism) analysis was conducted to cultivars of tobacco, Nicotiana tabacum in order to select the cultivar-specific markers. AFLP results using 12 primer sets revealed genetic diversity among 12 field grown tobacco cultivars. Polymorphic fragments amplified by PCR was purified and cloned to identify their nucleotide sequences. From the sequences of them, 40 primer sets were designed to select cultivar-specific markers. When genomic DNA isolated from tobacco were used as PCR template, a set of primers, BrSF/BrSR showed Burley-specific band patterns. The results indicate that AFLP technique used in this experiments is useful for identifying tobacco cultivars in a rapid manner.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.2
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• pp.258-261
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• 1983

This experiment was conducted to evaluate the effect of early seed harvest on germination of tobacco(Nicotiana tabacum L.) seeds. Seeds of seven burley tobacco were harvested every two days from 8 to 30 days after pollination and tested for germination. The results are; 1. Seeds harvested 12days after pollination germinated but germination rates were low and varied across cultivars. These seeds will provide viable seeds adequate for breeding program. 2. Germination rates of seeds harvested 24-28 days after pollination were high and showed none significant differences among cultivars. 4. Over-ripen seeds showed low germination rates cused by after-harvest or temporary dormancy.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.2
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• pp.109-113
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• 1995

Nicotiana repanda has resistance to many important tobacco diseases but no tobacco cultivars are currently available that carry risistance genes derived from this species. Numerous attempts to hybridize N, repanda with commercial tobacco cultivars have been largely unsuccessful because of cross incompatibility or the uncovering of lethal genes. In vitro pollination of placenta attached ovules was useful in by passing prezygotic barriers for interspecific hybrid combination between N.tabacum cv. NC82 and N. repanda. Six days after in vitro pollination of N. tabacum cv. NC82×N. repanda, enlarged ovules on plancenta were removed and transferred into ovule culture medium of kitsch and kitsch (1969). Within 15 days of ovule culture, germination occurred. Most of the hybrid seedlings obtained had poor root system and finally died, while few of them had good root system and grew well.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.1
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• pp.34-42
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• 1994

Experiments on the variability of plant type and factors representing the productivity and quality of the cultivars were subsequently carried out in relation to different plant density. Results are summarized as below. The higher was the plant density, the greater was the plant height and mean leaf inclination(MLI). As the plant density increased, the horizontal leaf area distribution became greater in proximal to the stem in NTN 77 and Br.49 but evenly in distal part in Br.21. Meanwhile, in terms of vertical leaf area distribution, it was decreased much more in middle and lower leaves than in upper in NTN 77 and Br.49 although it steadily decreased in any part of the plant in Br.21. Br.49 was the largest but Br.21 and NTN 77 were similar with respect to both CGR and NAR. The yield was greater in larger MLI cultivars(NTN 77>Br.49>Br.21). These three characteristics became greater when the tobacco were planted more densely in the field. Dry leaf weight and dry matter weight per plant were heavier in the larger MLI and increased with lower plant density. Total nitrogen content was greater in lower plant density and larger MLI cultivar. The plant density increases filling power seems to be enhanced regardless to the plant type. There was no discernible tendency on combustibility according to the plant density or plant type.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.371-375
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• 1998

Tobacco (Nicotiana tabacum) cultivars including NC 82 and KF 114, and Datura stramonium, Physalis floridana, Gomphrena globosa, and Chenopodium spp. were added to the previous host plants tested for the further examination on the biological characteristics of tobacco mosaic virus (TMV) strains isolated from tobacco (TMV-Common), tomato (TMV-Tomato), and pepper (TMV-Pepper), In TMV-Tomato and TMV-Pepper, different symptoms were noted in P. floridana (no symptom development), and NC 82 (local lesion production on the inoculated leaves) from TMV-Common with which systemic mosaic symptoms were developed. Local lesions were developed in KF 114, D. stramonium, G. globosa, and Chenopodium spp by TMV-Common and TVM-Tomato, while no symptom was observed in KF114 and G. globosa. Also the number and size of local lesions were smaller in KF 114 than Xanthi-nc tobacco (local lesion host) infected with TMV-Tomato. Systemic necrosis was induced in Xanthi-nc and KF 114 when infected with TMV-C at high temperature, but not with the other strains.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.2
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• pp.152-162
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• 1994

This study was designed to establish an analytical method for the properties of leaf tobacco smoke. Lyophilized TPM from leaf tobacco smoke was extracted with MeOH, dried under reduced pressure, and trimethyl-silylated(TMS). Gas chromatography of the material using SPB-5 column showed 120 quantifiable peaks. Among those, 26 compounds including a hydrocarbons, Neophytadiene. and Levulinic acid could be identified through GC-MS. Smoke properties of 5 manufacturing grades and 2 oriental cultivars of domestic and imported leaf tobacco including AB3O-1 were analyzed. For flue-cured tobacco, content of the compounds in the smoke was generally higher in American leaf tobacco except for glycerol compounds. For burley tobacco, domestic leaves were found to have much higher amount of smoke compound than imported leaves. Among oriental tobacco, Izmir contained slightly higher amount of smoke compounds than Basma. Key words : GC - profile. TPM. TMS. Leaf tobacco.