• Archives of Plastic Surgery
• /
• v.48 no.4
• /
• pp.448-456
• /
• 2021

Background Locoregional stem cell delivery is very important for increasing the efficiency of cell therapy. Amnisite BA (Amnisite) is a freeze-dried amniotic membrane harvested from bovine placenta. The objective of this study was to investigate the retention of cells of the stromal vascular fraction (SVF) on Amnisite and to determine the effects of cell-loaded Amnisite in a porcine radiation-induced chronic wound model. Methods Initially, experiments were conducted to find the most suitable hydration and incubation conditions for the attachment of SVF cells extracted from pig fat to Amnisite. Before seeding, SVFs were labeled with PKH67. The SVF cell-loaded Amnisite (group S), Amnisite only (group A), and polyurethane foam (group C) were applied to treat radiation-induced chronic wounds in a porcine model. Biopsy was performed at 10, 14, and 21 days post-operation for histological analysis. Results Retaining the SVF on Amnisite required 30 minutes for hydration and 1 hour for incubation. A PKH67 fluorescence study showed that Amnisite successfully delivered the SVF to the wounds. In histological analysis, group S showed increased re-epithelialization and revascularization with decreased inflammation at 10 days post-operation. Conclusions SVFs had acceptable adherence on hydrated Amnisite, with successful cell delivery to a radiation-induced chronic wound model.

• Imaging Science in Dentistry
• /
• v.52 no.3
• /
• pp.327-332
• /
• 2022

Mucocele of the paranasal sinuses is a benign, slow-growing, expansile lesion. Maxillary sinus mucoceles are usually associated with painless bulging of the cheek; however, orbital expansion is rarely observed. Maxillary sinus mucoceles can be classified as primary or secondary according to their etiology. An impediment to sinus ostium ventilation is thought to be the cause of primary mucocele, while sequestering of residual mucosa after surgery in the wound and long-term retention of tissue fluid have been suggested to lead to the formation of secondary mucocele. This report presents 2 cases of primary and secondary mucoceles, with a focus on radiographic features. As primary and superiorly positioned secondary maxillary sinus mucoceles are uncommon and their close proximity to the orbit predisposes the patient to significant morbidity, the authors expect that this report will contribute to a better understanding and diagnosis of maxillary sinus mucocele involving the orbit.

• Journal of Yeungnam Medical Science
• /
• v.23 no.2
• /
• pp.205-212
• /
• 2006

Background: Malignant pulmonary nodules account for about 30 to 40 percent of solitary pulmonary nodules (SPN). Therefore, tissue characterization of SPNs is very important. Recently, PET/CT has been widely used for tissue characterization, and has become of importance. The purpose of this study was to compare and to assess multiple factors in PET/CT comparing benign and malignant nodules. Materials and Method: Nineteen patients with SPN underwent PET/CT and biopsy. The difference of standardized uptake value 1 (SUV1), standardized uptake value 2 (SUV2) and retention index in PET/CT between malignancy and benignancy were compared by Levene's test. Result: There were twelve malignant and seven benign nodules. SUV1 and SUV2 were significantly different between malignant nodule and benign nodule (p=0.006 and 0.022), but retention index was not significantly different between malignant nodule and benign nodule (p=0.526). By receiver-operating-characteristic (ROC) analysis, the sensitivity was 66.7% and the specificity was 71.4% at a cut off value of 5.40 in SUV1. The sensitivity was 75% and the specificity was 71.4% at cut off value of 7.45 in SUV2. Conclusion: There was a statistically significant difference in SUV1 and SUV2 between benign and malignant nodules. However, the cut off value of SUV1 and SUV2 by receiver-operating-characteristic (ROC) analysis was 5.40 and 7.45 which is different from previous studies. Therefore, studies on a larger sample of patients are required for confirmation.

• Polymer(Korea)
• /
• v.34 no.3
• /
• pp.282-288
• /
• 2010

The porcine small intestinal submucosa (SIS) has been widely used as a biomaterial without immuno rejection responses and hyalunonic acid (HA) can be used as biocompatible materials to regenerate tissue. We developed the SIS sponge and HA loaded SIS sponges (SIS/HA) for the possibility of the application of the tissue engineering using annulus fibrosus (AF). SEM observation shows that SIS and SIS/HA sponges have interconnected and open pores. We demonstrated the presence of HA in SIS/HA sponge from C-O functional group observed by the FTIR analysis. In conclusion, we suggest that SIS/HA sponge may be useful to tissue engineering using AF cell. This may be due to the enhanced biocompatibility and higher water retention capacity of HA.

• The korean journal of orthodontics
• /
• v.28 no.3 s.68
• /
• pp.419-429
• /
• 1998

This study was carried out in order to study early histologic changes and repair reaction appling to extrusive force for 3rd premolar of adult dogs. After 1 week of extrusive force with elastic chain, one of dogs was sacrified and after 3 weeks retention period, another dog was sacrified. The paraffin sections of samples were stained with Hematoxylin - Eosin and Masson's Trichrome and were examed by light microscopy . The obtained results as follows 1. In Hematoxylin - Eosin and Masson Trichrome stain of control group , the periodontal ligament width was constant from apical third to cervical third of the root and periodontal fiber arrangement was horizontal or oblique in cervical third. oblique in middle third, oblique in apical third of root. in alveolar bone, smooth appearance was shown 2. In Group 1, all periodontal fiber arrangement was oblique toward tooth, and the periodontal ligament width increased Partially PDL was ruptured in apex. In MT stain, immature bone formation was seen at alveolar crest area. Active bone formation was observed along the one side of alveolus, and apical portion of pulp was involved with blood vessel rupture , vacuolization of pulp tissue and hyperemia 3. In Group 2, most periodontal ligament arrangement and PDL width was repaired and fiber density increased. In MT stain, mineralization of immature bone on the alveolar crest was progressed. In pulp, vacuole and hyperemia was diminished and fibrotic change was diminished 4. After 3 week periodontal ligament has more repair ability than pulp tissue. pulp was involved with vacuolization and fibrosis, so it takes more time for repair.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.41 no.4
• /
• pp.328-334
• /
• 2014

Gingival fibromatosis is a rare oral condition that is characterized by proliferative fibrous overgrowth of the attached gingiva, the marginal gingiva, and the interdental papilla, typically presenting in the growth period. A case of a 27-month-old girl with a generalized severe gingival overgrowth is described herein. The patient had no known systemic disease, but enlarged gingival tissue had gradually covered her teeth. The excess gingival tissue was removed by conventional gingivectomy, which involved extraction of the retentive primary teeth under general anesthesia when she was 5 years old. Post surgical follow-up at 18 months after the surgery demonstrated no recurrence. Resectional surgery of the enlarged gingival tissue is the treatment choice for gingival fibromatosis, although there is a high risk of recurrence. More frequent professional follow-ups and oral hygiene instruction might be required. A delay in the surgical treatment may have significant consequences for the patient, such as primary dentition retention and consequent delay in the eruption of the permanent teeth, difficulties in mastication and phonation, malpositioning of the teeth, and psychological problems. Early surgical treatment should be performed according to the severity of enlargement.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.33 no.1
• /
• pp.139-145
• /
• 2006

Ranula is a mucocele which occurs at the mouth floor and is mostly related to sublingual gland. In other words ranula is definded as a pseudocyst which occurs as the secretion duct of sublingual gland is destructed there as the saliva from the secretion duct flows out and retention in the soft tissue. The cause of ranula is destruction or obstruction of the duct. The clinical findings of ranula is a painless, unilateral bluish transparent swelling around the frenum and shows fluctuation when palpated. Histological finding represent a formation of cavity inside the connective tissue, but a pseudo-cyst can be seen which the wall of the cyst is composed of granulation tissue rather than epithelial cells. The first treatment of ranula can be considered as marsupialization. which induces the inner wall of the Ranula to be a part of oral mucosa. This case report shows a treatment of marsupialization with gauze packing in a young patient representing a clinical finding of characteristic ranula.

• Journal of Animal Science and Technology
• /
• v.54 no.4
• /
• pp.307-313
• /
• 2012

This study was conducted to investigate the effects of glucomannan (GMN), carrageenan (CAR), carboxymethyl cellulose (CMC), and transglutaminase-B (TGB) on the quality and storage properties of pork patties manufactured with pork skin connective tissue during 21 d of storage at $4^{\circ}C$. Results showed that CIE color values like lightness, redness and yellowness did not differ significantly among the pork patties. Sensory attributes also did not differ between the treatments (P>0.05). However, cooking loss was significantly lower in the group with added GMN, CAR, CMC, and TGB compared to the control at 21 d of storage. The shear force value of GMN and TGB were lower than the control at 21 d of storage (P<0.05). The pork patties added with GMN and TGB had lower thiobarbituric acid reactive substances (TBARS) values than the control at 1 or 21 d of storage (P<0.05). Volatile basic nitrogen (VBN) values of all treatment samples was lower than the control at 21 d of storage (P<0.05). Therefore, result of cooking loss suggested that the decrease in shear force in GMN and TGB were due to higher moisture retention. Also, the pork skin connective tissue with added GMN and TGB decreased lipid oxidation of pork patties.

• Horticultural Science & Technology
• /
• v.33 no.2
• /
• pp.219-226
• /
• 2015

The objective of this research was to investigate the influence of various root media on the growth of mother and daughter plants during propagation and early yield after transplanting of 'Seolhyang' strawberry. To achieve this, daughter plants were fixed to connected small pots that contained expanded rice-hull (ERH), a strawberry-specialized commercial medium (SSCM), soil mother materials (SMM), or loamy sand (LS). Then, growth of daughter plants in above- and below-ground tissue as well as early yield after transplanting to plastic house soil were investigated. The growth of daughter plants in terms of plant height, leaf area and fresh weight were the highest in the SSCM treatment. Root growth in terms of the amount of primary roots and root dry weight were the highest in the treatments of ERH and SMM and the lowest in that of SSCM, among treatments tested. The ERH treatment also showed the highest values among treatments in root length, surface area and volume when roots with 0 to 0.4 mm in diameter were investigated. The flower bud differentiation of daughter plants began on Sept. 3 in the ERH treatments, earlier than the SMM (Sept. 5) and in SSCM (Sept. 7) treatments. The tissue N contents of daughter plants were in the range of 1.41 to 1.55% in all treatments, and no significant differences were observed among treatments. This indicates that the low moisture retention capacity of ERH and water stress, rather than tissue N contents, promote the flower differentiation of daughter plants. In the evaluation of early yield after transplant, the ERH treatment of showed the highest yield in the period from November to December, reaching 667 g fruit weight per 10 plants. The yields per 10 plants in the other treatments were 581 g in SMM, 475 g in SSCM and 295 g in LS. Above results imply that the various root media have different effects on the growth of daughter plants as well as flower bud differentiation. Therefore, improvement in early yield after transplant can be achieved through selection of proper root medium for daughter plant propagation.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.36 no.1
• /
• pp.150-156
• /
• 2009

Objectives : The conventional removable appliance, composed of wires and acrylic resin, had unaesthetic results and poor retention. The flexible denture, as an alternative, presents improved aesthetics with the thin and strong resin retentive area. In addition, it also enhances patients' sensory function as a result of decreased volume of denture base. The flexibility of the flexible denture reduces the possibility of fracture and distributes the masticatory forces transmitted to the abutments and residual bone tissue. This report describes a 10-year-old girl and a 6-year-old boy with oligodontia treated with the flexible dentures as an alternative to conventional removable appliances. Methods : Impression was taken using alginate material and sent to a laboratory with the bite for fabrication of the flexible denture. Prior to try-in, the flexible denture was immersed in water at $90^{\circ}C$ for one minute and cooled. Impinging area of the denture was checked by $Fit-Checker^{(R)}$ and removed and the denture was delivered to the patient. Results : Both patients were satisfied with the flexible dentures, which presented improved retention and aesthetics. Conclusion : For patients with oligodontia, flexible dentures can be considered as a treatment of choice, which may replace the conventional denture.