• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.293-295
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• 2003

Janczewskia (Rhodomelaceae, Rhodophyta), the parasitic red algal genus, was established by Solms-Laubach (1877). This genus is characterized by wart-like thallus with coalescent branches, which form a solid tissue. Some species have conspicuously projected branches, but others have not this kind of branches. In the species, the branches rarely project beyond the surface of the nodule-like body. (omitted)

• Journal of International Society for Simulation Surgery
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• 제3권1호
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• pp.33-35
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• 2016

Fibrous dysplasia(FD) is a disorder in which normal bone is replaced with pathologic tissue. When occurring in craniofacial regions, the zygomaticomaxillary complex is most commonly affected and this pathologic lesion results in facial asymmetry. and By using computer-assisted virtual simulation, precise maxillofacial contouring was achieved for harmonious facial morphology and the surgical procedure was simplified and the surgery brought satisfactory results in terms of both esthetics and functionality.

• Applied Microscopy
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• 제50권
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• pp.10.1-10.6
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• 2020

Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2009년도 추계학술발표대회
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• pp.38.2-38.2
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• 2009

Regeration of natural tissuesor to create biological substitutes for defective or lost tissues and organs through the use of cells. In addition to cells and their porous, drugs are required to promote tissue regeneration. Therefore, the present studies were prepared using simvastatim loaded porous poly(lactide-co-glycolide) (PLGA) by double emulsion solvent evaporation water-in-oil-in-water technique (W/O/W) as drug delivery system strategies for injuring tissue. The resulting microspheres were evaluated for morphology, particle size, encapsulation efficiency, degradation of PLGA microspheres in vitro drug release and in vitro cell viability. Scanning electronic microscopic (SEM) showed that the porosities of the particles was changed by experimental conditions and cultured cells were attached well on porous microspheres surface. The X-ray diffraction (XRD) and differential scanning calometry (DSC) analysis indicate thatsimvastatim was highly dipersed in the microsphere at amorphousstate.

• 대한의생명과학회지
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• 제16권1호
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• pp.33-38
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• 2010

Culinary herbs and spices have received much attention since they contain high concentrations of bioactive ingredients for antioxidant and anti-inflammatory activities. Protection effect of the herb and spice against acute alcohol consumption has been investigated using zebrafish as a vertebrate model. During 30 days bathed in water containing 1% Et-OH and the designated herb or spice, the survival rate of the Et-OH group was decreased sharply (up to 20% at 10 days). The cinnamon-fed group showed the highest and longer survival rate up to 80% up to for 30 days under the presence of Et-OH, while clove-fed group showed 40% survival rate for 25 days. Et-OH group serum exhibited the weakest antioxidant ability from ferric ion removal ability (FRA) assay; FRA ability was increased in the cinnamon-fed group up to 414%, while the clove and laurel group increased 256% and 309%, respectively. Histologic observation and Oil-red O staining showed hepatic tissue damage was severe in the Et-OH group. The cinnamon- or clove-fed group showed much ameliorated hepatic tissue morphology with minimized steatosis. The cinnamon- or clove-fed group showed lower serum GOT and GPT levels than the Et-OH group. Among hepatic tissue extract, the clove-fed group exhibited the lowest level of GOT and GPT. These results suggest that consumption of cinnamon and clove might be beneficial to attenuate progress of acute fatty liver change by alcohol consumption.

• Archives of Pharmacal Research
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• 제29권9호
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• pp.800-807
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• 2006

This study examined the possibility of using a tissue cultured root of wild Panax ginseng (tcwPG) as a fertility agent. The effect of tcwPG on spermatogenesis was studied using male rats. The tcwPG crude powder was administered orally to 7-week-old rats over a 6-week period. The number of sperm in the testes and epididymides was significantly higher than the control. A histological examination did not reveal any morphological changes in the testes from the tcwPG powder treated rats. Moreover, there were no significant differences in the weights of the heart, spleen, liver, kidney, brain, testes and epididymides. Oligospermia was also induced by administering 2,3,7,8-tetrachlorodaibenzo-p-dioxin (TCDD) to the rats in order to estimate the feasibility of using tcwPG as treatment for infertility caused by spermatogenic disorders. After exposing the rats to TCDD, the tcwPG saponin fraction treated rats showed some improvement in the body weight, sperm number and testis morphology. It was estimated that tcwPG had feasibility as a therapeutic agent on spermatogenic disorder.

• Fibers and Polymers
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• 제2권2호
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• pp.64-70
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• 2001

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

• 대한치과교정학회지
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• 제15권2호
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• pp.175-195
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• 1985

Vertical and horizontal growth occur in the craniofacial complex which ensues continuous changes in facial morphology, until the end of active growth period. Longitudinal study for individual is essential, in the research on growth and development, however, the difficulties in obtaining long term subjects in Korea, the research has been limited. The author analyzed the cephalometric roentgenogrems of 43 boys and 47 girls taken from the ages 6 to 10. The subjects were divided into 3 groups according to SN-MP angle and 2 groups according to gonial angle. In this longitudinal study, 21 variables were measure 4. The obtained results were as follows: 1. SN-MP angle and genial angle had no significant changes in each group with age. 2. With age, facial convexity of hard tissue decreased in all groups, facial angle of hard tissue increased in low SN-MP angle group, but facial convexity of soft tissue had no significant changes in all groups with age. 3. In comparison of high SN-MP angle group and low SN-MP angle group, the former had greater facial convexity and smaller facial angle than the latter. 4. SN-MP angle and the ratio of posterior dental height to anterior dental height had reverse correlation in all groups. 5. High genial angle group revealed larger SN-MP angle, anterior dental height facial convexity, but smaller mandibular length, and the ratio of posterior dental height to anterior dental height compared with low genial angle group.

• 융합신호처리학회논문지
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• 제2권4호
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• pp.22-30
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• 2001

본 논문은 유방질환 중에서 유관(duct )에 발생하는 유방종양을 Benign, DCIS(ductal carcinoma in situ) NOS (invasive ductal carcinoma)로 분류하기 위해 3가지 분류기 (classifier) 를 생성한 후, 비교 분석하였다. 분류기 생성에서 가장 중요한 단계인 특징 추출 단계에서 세포핵의 기하학적 특징을 형태학적 특징을 추출하여 분류기를 생성하고 염색질 패턴의 내부적 변화를 나타내는 질감 특징을 추출하여 2가지 배율(100/400배)에서 2개의 분류기를 생성하였다. 400배 배율의 유방질환 영상에서 세포핵을 추출하여 핵의 형태학적 특징값인 핵의 면적, 둘레. 가로, 세로(장. 단축) 의 길이, 원형성의 비율을 구한 후 이 특징값들을 조합하여 판별분석에 의해 분류기를 생생하고, 분류 정확도를 검증하였다. 100배 배율과 400배의 배율의 유방질환 영상에서 1, 2, 3, 4 단계(level)의 wavelet 변환를 적용한 후, 분할된 서브밴드에서 GLCM(Gray Level Co-occurrence Matrix)을 이용하여 질감 특징(entropy Energy, Contrast, Homogeneity)를 추출하고, 이 특징값들을 조합하여 판변 분석에 의해 분류기를 생성한 후 분류 정확도를 검증하였다. 이 세 분류기를 비교 분석 하였을때 현민경 100배 배율의 영상을 3단계 wavelet 변환을 적용하고 질감 특징을 추출하여 생성한 분류기가 다른 두 분류기보다 유방 질환 Benign, DCIS; NOS를 분류하는데 더 나은 결과를 보였다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권11호
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• pp.1532-1536
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• 2015

To verified the target genes of miR-34c, bioinformatics software was used to predict the targets of miR-34c. Three possible target genes of miR-34c related to spermatogenesis and male reproductive development: zinc finger protein 148 (ZNF148), kruppel-like factor 4 (KLF4), and platelet-derived growth factor receptor alpha (PDGFRA) were predicted. Then, the expression of miR-34c and its target genes were detected in swine testicular tissue at different developmental stages by quantitative polymerase chain reaction. The results suggested that the expression of PDGFRA has the highest negative correlation with miR-34c. Then immunohistochemical staining was done to observe the morphology of swine testicular tissue at 2-days and 3, 4, 5-months of age, which indicated that PDGFRA was mainly expressed in the support cells near the basement membrane during the early development stages of testicular tissue, but that the expression of PDGFRA was gradually reduced in later stages. Therefore, western blot analyzed that the highest expression of PDGFRA was generated in 2-days old testicular tissues and the expression levels reduced at 3 and 4-months old, which correlated with the results of immunohistochemical staining. In conclusion, PDGFRA is a target gene of miR-34c.