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Biochemical Activity of Microorganism Stored by the Method of Partial Freezing (부분동결시에 미생물의 생화학적 활성에 관한 연구)

  • CHO Young-Je
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.3
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    • pp.185-190
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    • 1987
  • In order to study the preservation effect of $-3^{\circ}C$ partial freezing method, tile growth and biochemical activity of microorganisms and the changes of K-value in mackerel press juice were investigated at $0^{\circ}C,\;-3^{\circ}C$ supercooling (liquid phase) and $-3^{\circ}C$ freezing (solid phase). The results obtained in this paper were follows : 1) The growth and biochemical activity of microorganisms were reduced at $-3^{\circ}C$ supercooling than $0^{\circ}C$ in spite of the small variation in temperature. 2) There were no growth and biochemical activity of microorganisms at $-3^{\circ}C$ freezing (solid phase). 3) The difference in the K-value between $-3^{\circ}C$ supercooling and $-3^{\circ}C$ freezing was remarkable in spite of the same temperature.

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Development of Profile Technique for Steam Generator Tubes in Nuclear Power Plants Using $8{\times}1$ Multi-Array Eddy Current Probe ($8{\times}1$ 다중코일 와전류탐촉자를 이용한 원전 증기발생기 전열관 단면형상검사 기법 개발)

  • Nam, Min-Woo;Lee, Hee-Jong;Kim, Cheol-Gi
    • Journal of the Korean Society for Nondestructive Testing
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    • v.28 no.2
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    • pp.184-190
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    • 2008
  • Various ECT techniques have been applied basically to assess the integrity of steam generator tithing in nuclear power plant. Among these techniques, the bobbin probe technique is applied generally to examine the volumetric flaws such as a crack-like defect and wear which is generally occurred on steam generator tubing, and additionally MRPC probe is used to examine closely tile top of tubesheet and bending regions due to the high possibility of cracking. Dent and bulge also may be formed on tube during installation process and operation of steam generator, but the dent and bulge indications greater than specific size criteria are recorded on examination report because these indications are not considered as flaw. These indications can be easily detected with bobbin probe and approximately sized with profile bobbin probe, but the size and shape can not be accurately verified. Accordingly, in this study, the $8{\times}1$ multi-array EC probe was designed to increase the measurement accuracy of the sectional profiling EC testing of tube. As a result, we would like to propose the application of $8{\times}1$ multi-array EC probe for the measurement of size and shape of profile change on steam generator tube in OPR-1000 nuclear power plant.

Organic Matter Sources in a Reservoir (Lake Soyang); Primary Production of Phytoplankton and DOC, and External Loading (식물플랑크톤의 세포외배출유기물을 고려한 소양호의 1차생산과 유기물 부하)

  • Nam, Kung-Hyun;Hwang, Gil-Son;Choi, Kwang-Soon;Kim, Chul-Goo;Kim, Bom-Chul
    • Korean Journal of Ecology and Environment
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    • v.34 no.3 s.95
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    • pp.166-174
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    • 2001
  • The autochthonous and allochthonous organic carbon loading were measured in Lake Soyang, to estimate the amount of carbon loading into the lake and the contribution of their sources to tile lake's carbon loading. Autochthonous carbon loading was estimated from phytoplankton primary production with the extracellular organic carbon (EOC). Allochthonous loading was determined by measuring dissolved organic carbon (DOC) and particulate organic carbon (POC) concentration in the main inflowing Soyang River. Both autochthonous and allochthonous organic carbon loading were high during the svmmer, from July to September, and accounted for 43.2% and 71.7% of the annual loading, respectively. Primary productivity was elevated up to $1,000\;mgC\;m^{-2}\;d^{-1}$ during summer and lowest in winter. EOC production from phytoplankton was also large in summer, resulting in a high DOC concentration in the lake water. Primary production of phytoplankton and allochthonous organic matter loading from the watershed contributed to 53.6% and 46.4% of total loading, respectively. The EOC production accounted far $4.4{\sim}21.2%$ of POC primary production, implying that EOC production of phytolankton must be considered in estimation of primary production.

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Studies on Eriophyes kuko KISHIDA and its Galls -On the Activity of Enzymes and the Chemical Constituents in Growing Galls- (구기자혹응애(Eriophyes ruro KISHIDA) 및 그 혹(Gall)에 관한 연구 II. 혹의 성장에 따르는 효소활성 및 화학성분에 대하여)

  • Kim Chang Hyo;Ki Woo Kyung;Sung Nack Kie;Park Woo Churl
    • Korean journal of applied entomology
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    • v.10 no.1
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    • pp.23-30
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    • 1971
  • This work was carried out to study the chemical properties, the activity of several enzymes, chemical components and the respiratory intensity of mite galls on the leaves of Lycium chinense MILL. caused by Eriophyes kuke KISHIDA. The activities of catalase and peroxidase were higher in the gall tissue, when compared to the healthy tissue, and were increased as tile gall developed. The activity of phosphorylase of the healthy tissue seemed to be higher than that of the large gall l tissue, considering the competitive inhibition of phosphomonoeoterase and $\beta-amylase$, The activities of invertase and $\beta-amylase$ were about two times higher in the large sail tissue than those of the healthy tissue. The content of the crude protein was $20\%$ higher in the small gall tissue than that of the healthy leave tissue, and decreased as the gall matured. On the other hand, the reducing sugar level was less in the small gall tissue than that of the healthy leaves, but as the gall grew, the content of reducing sugar of the gall tissue was increased. The contents of phosphorus and tannin were increased gradually as the gall matured, and their content showed about two times higher than those of the healthy. The matured gall tissue showed higher $QO_2$ than the healthy tissue. On the other hand, the matured gall tissue presented lower $QCO_2$ than the healthy tissue, and the RQ of the healthy tissue was higher than that of the gall tissue.

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A Survey of Potato Virus Diseases and Insect Vectors at Seed Potato Production Area in Jeon Bug(II). Serological Test on Virus Diseases of Seed Potatoes (전북지방의 씨감자 생산을 위한 적지선정 및 감자 바이러스병에 관한 연구(II). 씨감자의 바이러스병 검정)

  • Yoon Soon Kie;So In Yung;Choi Seong Shick
    • Korean journal of applied entomology
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    • v.14 no.2 s.23
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    • pp.71-76
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    • 1975
  • The study has been carried to find a proper site for seed potato production in Jeon Bug Province, Korea. Two fields, one at the alpine area (700-800 m sea level) in Muju county and the other at the plain area in Jeoniu, were chose for this study. Seed potatoes for the experiment were obtained from Alpine Experiment Station and from the traditional sources in Muju area. During the growing season the virus infection (Potato virus X,S,Y, and M) was detected by serolgical method. The average percent of virus infection on the original seed potatoes, which have been used as a seed source in Korea, was $50.5\%$, and the ratio of the infection for each of potato virus, Y, M and X was 34..87, 40.33, 41.00 and $87.10\%$, respectively. Infection percentage of virus at first year in Muju area was $11.7\%$ and those in Jeonju area was $18.95\%$. In case of potato virus Y, the most prevalent virus in Korea, lower Infection percentage $(6.45\%)$ was found in Muju area than in tile Jeonju area $(26.0\%)$ when the seed from Alpine Experiment Station was planted in both areas. The percentage of infected seed potato, Alpine Experiment Station$(41.75\%)$ was almost the same as those from the traditional sources in Muju $(42.17\%)$. The production of seed potato, however, was much better by the seeds from Alpine Experiment Station.

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On Feasibility of Ambulatory KDRGs for the Classification of Health Insurance Claims (KDRG를 이용한 건강보험 외래 진료비 분류 타당성)

  • 박하영;박기동;신영수
    • Health Policy and Management
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    • v.13 no.1
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    • pp.98-115
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    • 2003
  • Concerns about growing health insurance expenditures became a national Issue in 2001 when the National Health Insurance went into a deficit. Increases in spending for ambulatory care shared the largest portion of the problem. Methods and systems to control the spending should be developed and a system to measure case mix of providers is one of core components of the control system. The objectives of this article is to examine the feasibility of applying Korean Diagnosis Related Groups (KDRGs) to classify health insurance claims for ambulatory care and to identify problem areas of the classification. A database of 11,586,270 claims for ambulatory care delivered during January 2002 was obtained for the study, and the final number of claims analyzed was 8,319,494 after KDRG numbers were assigned to the data and records with an error KDRG were excluded from the study. The unit of analysis was a claim and resource use was measured by the sum of charges incurred during a month at a department of a hospital of at a clinic. Within group variance was assessed by th coefficient of variation (CV), and the classification accuracy was evaluated by the variance reduction achieved by the KDRG classification. The analyses were performed on both all and non-outlier data, and on a subset of the database to examine the validity of study results. Data were assigned to 787 KDRGs among 1,244 KDRGs defined in the classification system. For non-outlier data, 77.4% of KDRGs had a CV of charges from tertiary care hospitals less than 100% and 95.43% of KDRGs for data from clinics. The variance reduction achieved by the KDRG classification was 40.80% for non-outlier claims from tertiary care hospitals, 51.98% for general hospitals, 40.89% for hospitals, and 54.99% for clinics. Similar results were obtained from the analyses performed on a subset of the study database. The study results indicated that KDRGs developed for a classification of inpatient care could be used for ambulatory care, although there were areas where the classification should be refined. Its power to predict tile resource utilization showed a potential for its application to measure case mix of providers for monitoring and managing delivery of ambulatory care. The issue concerning the quality of diagnostic information contained in insurance claims remains to be improved, and significance of future studies for other classification systems based on visits or episodes is guaranteed.

Influences of Clearcutting of Timber Harvesting on Stream Water Quality (개벌에 의한 수확벌채가 계류수질에 미치는 영향)

  • Park, Jae-Hyeon
    • Korean Journal of Ecology and Environment
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    • v.35 no.1 s.97
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    • pp.45-51
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    • 2002
  • This study was investigated to tile influences of clearcutting of timber harvesting on stream water quality at a natural deciduous forest catchment within the Seoul National University Research Forests in Mt. Baegun, Chunnam province during the periods of 1993 to 2001. Soil chemical ingredients and stream water qualities were monitored at 13 ha clearcutting site, non-treatment site and included in these catchment nearby. After the harvesting first and End years, the levels of total- N, and exchangeable ions ($K^+,\;Na^+,\;Ca^{2+},\;Mg^{2+}$) were decreased the values of before harvesting, and after the harvesting 111th years and eighth years, respectively. But the chemical characteristics of soil were not changed at all. pH of water in the harvesting area was 6.5 in stream water. Among the nutrient, Cd, Pb, Cu, and phosphate were not found, and the level of BOD reached at the level of the domestic use for drinking. Turbidity, odor, taste, $NH_4\;^+-N,\;NO_3\;^--N$, standard plate count, and coliform were also low enough to be used as the domestic use for drinking in the near villagers. Total amount of cation, total amount of anion and total amount of ion in clearcutting site, non- treatment site and included in these catchment nearby were higher than clearcutting site and non- treatment site. But, there was no great difference.

A Study of School program in the Case of Library and Information Science in Korea (한국문헌정보학의 학부제 도입에 관한 연구)

  • Koo Bon-young
    • Journal of the Korean Society for Library and Information Science
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    • v.30 no.2
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    • pp.3-50
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    • 1996
  • At present, Korean universities and colleges are confronted with a strong challenge, within and without, demanding a drastic change or innovation of university education. Discussion on the propulsion of the School Program is now under way as one of the means to reform the university education With such a background in mind, we have considered what should be the desirable course of Library and Information Science in Korea. We have considered the characteristic features and roles of library and information professionals to cope with the now approaching information society and the changing conditons of educational environment We have collected the pros and cons among professors of tile School Program, and investigated the curricula of the three universities which are at present practicing the School Program. Of the 139 professors to whom we have sent the questionnaries, $92(66\%)$ responded, of which the pros were 42 and the cons were 45. The model plan for the School Program is 'departmental school program' $(44\%)$, which purports to adopt 'departmental school program', with the curricula and material resources being utilized in common, or to adopt joint-appointment system with the common utilization of teaching materials and the introduction of a limited dual major system. The argument against introduction the School Program was that because of the peculiar nature of the discipline itself, Library and Information Science can not be merged with any other discipline.

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Effect of 2,4-Dichorophenoxyacetic Acid on Adventitious Root Formation from Callus Bupleurum falcatum L. and Its Histological Observation (시호(Bupleurum falcatum L.)의 캘러스로부터 부정근 분화에 미치는 2,4-Dichlorophenoxyacetic Acid의 영향과 분화의 해부학적 고찰)

  • 배형화;조덕이;김성길;소웅영;성낙선
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.1
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    • pp.41-46
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    • 1994
  • Calli were induced from leaf explants of B.falcatum, and selected cell clumps of the calli (900-1, 000${\mu}{\textrm}{m}$) were cultured on MS medium supplemented with 0.1, 0.5, 1.0 or 2.0 mg/L 2, 4-D for 7 days, respectively: The clumps were subsequently transferred onto MS basal medium and subcultured for four weeks. In order to investigate the effect of 2, 4-D pretreatment, the selected clumps were cultured on MS medium supplemented with 0.1 mg/L 2, 4-D for 24, 48, 72, 96, 120 or 144 hours and then transferred to liquid MS basal medium, wherein they were cultured for 4 weeks. Histological observation showed that root initial cells were developed from cells on the surface of clumps or from cells in the inner region. Clumps on the basal medium produced mot within 5 days of culture. The rate of prutruding time was inversely proportional to the concentration of 2, 4-D. The number of adventitious roots per clump preheated with 0.1 mg/L 2, 4-D was an average of 5.2, which was the highest level. On MS medium as control, the clumps formed 3.3 adventitious roots each. As tile concentration of 2, 4-D increased, the number of adventitious roots were declined accordingly: The number of adventitious roots as the period of pretreatment increased upto 120 h.

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Complement-dependent Cytotoxic Crossmatch for Prevention of Hyperacute Rejection in Canine Renal Allografts (개의 신장 동종 이식에서 초급성 거부반응의 예방을 위한 보체의존성 세포독성 DLA 플래스 I 교차 반응)

  • Ghil Heh Myung;Woo Heung-Myung
    • Journal of Veterinary Clinics
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    • v.22 no.4
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    • pp.322-327
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    • 2005
  • DLA class I complement-dependent cytotoxicity (CDC) cross-match method was established to control hyperacute rejections in organ transplantation. The aim of the present study is to investigate if DLA class 1 CDC corssmatch method is effective to prevent hyperacute rejections in canine renal allografts. Seven mongrel dogs of similar age and weight were used. Erythrocyte crossmatch was first performed and only the negatives were used. Among the same blood types, CDC cross-match was performed. Anti-dog serum, Hank's balanced salt solution(HBSS), and tile self-serum was used as a positive-, a negative-, and all auto-control respectively. After the reaction with class I complement, it was stained with eosin and scored by international cytotoxicity scoring system under inverted phase microscope. According to these results, kidneys oi CDC negatives among same blood types were cross-transplanted to observe the incidence of hyperacute rejections. One of four 1.2 B blood type dogs had autoantibodies. here were negative CDC results among each blood type, and also there were negative results between different blood types. Two pairs with the same blood types and negative CDC results underwent allo-transplantation each other. There were no hyperacute rejections. DLA cross-match method studied in this experiment for canine renal allograft can be effective to prevent hyperacute rejections. it may be applicable for the future studies of histocompatibility testing in canine renal allografts.