• 제목/요약/키워드: three species

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천남성(天南星) 유전자 감별을 위한 DNA 바코드 분석 및 Marker Nucleotide 발굴 (Identification of Marker Nucleotides for the Molecular Authentication of Arisaematis Rhizoma Based on the DNA Barcode Sequences)

  • 김욱진;이영미;지윤의;강영민;최고야;김호경;문병철
    • 대한본초학회지
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    • 제29권6호
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    • pp.35-43
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    • 2014
  • Objectives : Official Arisaematis Rhizoma is described only three species, Arisaema amurnse, Arisaema erubescens, and Arisaema heterophyllum, in national Pharmacopoeia. However, other Arisaema species, Arisaema ringens, Arisaema takesimense and Arisaema serratum, also have been distributed as an inauthentic Arisaematis Rhizoma in the herbal market. To develop a reliable molecular authentication method for Arisaematis Rhizoma in species level, we analyzed DNA barcode regions using six Arisaema species. Methods : Thirty-eight samples of six Arisaema plants species (A. amurense, A. amurense f. serratum, A. heterophyllum, A. takesimense, and A. serratum) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL gene) were analyzed after PCR amplification. The species-specific sequences and phylogenetic relations were estimated using entire sequences of three DNA barcodes based on the analysis of ClastalW and UPGMA, respectively. Results : The comparative analysis of DNA barcode sequences were revealed inter-species specific nucleotides to distinguish the medicinal plant of Arisaema Rhizoma in species levels excluding between A. amurense and its subspecies (A. amurense f. serratum) and A. takesimense and A. serratum, respectively. However, we obtained sequence differences enough to discriminate authentic and inauthentic Arisaematis Rhizoma. Therefore, we suggest that these SNP type molecular genetic markers were an reliable method avaliable to identify official herbal medicines. Conclusions : These marker nucleotides could be useful to identify the official herbal medicines by providing definitive information that can identify original medicinal plant and distinguish from inauthentic adulterants and substitutes.

우리나라 건축물에 사용된 목재 수종의 변천 (Changes in the Species of Woods Used for Korean Ancient and Historic Architectures)

  • 박원규;이광희
    • 건축역사연구
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    • 제16권1호
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    • pp.9-28
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    • 2007
  • We investigated the changes in the species of the woods used for Korean ancient and historic architectures, which include prehistoric excavated relics and existing wooden buildings in South Korea. The species data were collected from various sources such as excavation and repair reports, journal papers, and a few unpublished documents. We divided the building Periods as Paleolithic, Neolitic, Bronze Ages, Iron Age/Three Kingdoms, Koryo, Joseon (early, middle, late) and modem periods. In prehistoric periods, hardwoods were major species. Oak (Quercus spp.) woods dominated (94 percent in average); the others (5%) were Juglans mandshurica, Platycarya strobilacea, Castanea crenata, and few softwoods(1%). During Iron Age and Three Kingdom periods, oaks remained as a major species (57%) and others Platycarya strobilacea(21%), Castanea crenata(13%), and Pinus spp. (6%). The oak woods decreased in Koryo period and they occupied only 1.1%. Instead of oaks, pine (Pinus spp., 71%) and Zelkova serrata (22%) dominated in Koryo. In early and middle Joseon periods, pine woods (73%) remain as a major species and the others were oaks (14%) and Zelkova serrata (9%). As late Joseon came, the pine woods occupied more than 88%. In the late 19th and early 20th centuries, a few boreal species such as larch (Larix spp.) and spruce (Picea spp.), which grow in cold area, were found. We believe they were transported from northern Korea. The existing buildings in Korea are mainly from Joseon period and a few from late Koryo periods. During these periods, pine woods were used for most buildings. For such reason, pine woods were known as 'representative materials for historic buildings'. but earlier times, broad-leaved trees, i.e., oak and Zelkova woods were major materials. The changes in building materials resulted from both climate and human impacts. The dry climate and disturbed forests induce more pines in the mountains. We also compared the wood qualities of the species and found that Zelkova woods were superior ones and deserved more planting for future demands in the repair for historic buildings.

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정읍 신정동 연구단지 및 RFT산업단지 주변 외래식물 현황 (Exotic Plant Species around Jeongeup Research Complex and RFT Industrial Complex)

  • 김진규;차민경;류태호;이윤종;김진홍
    • 방사선산업학회지
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    • 제9권3호
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    • pp.119-126
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    • 2015
  • In Shinjeong-dong of Jeongeup, there are three government-supported research institutes and an RFT industrial complex which is currently being established. Increased human activities can affect flora and fauna as a man-made pressure onto the region. As a baseline study, status of exotic plants was investigated prior to a full operation of the RFT industrial complex. A total of 54 species and 1 variety of naturalized or introduced plants were found in the study area. Among them, three species (Ambrosia artemisifolia var. elatior, Rumex acetocella and Aster pilosus) belong to 'nuisance species', and four species(Phytolacca americana, Iopomoea hederacea, Ereechtites hieracifolia and Rudbeckia laciniata) to 'monitor species' designated by the ministry of Environment. Some of naturalized trees and plants were intentionally introduced in this area, while others naturally immigrated. Physalis angulata seemes to immigrate in the study area in the form of mixture with animal feeds as its distribution coincided with the transportation route of the animal feeds. Liquidambar styraciflua is amenable to the ecological investigation on the possible expansion of the species to the nearby Naejang National Park as its leave shape and autumn color are very similar to those of maple trees. The number of naturalized plants around the RFT industrial complex will increase with an increase in floating population, in human activities in association with constructions of factories and operations of the complex. The result of this study provides baseline data for assessing the ecological change of the region according to the operation of the RFT industrial complex.

Taxonomic Study of the Genus Pholiota (Strophariaceae, Basidiomycota) in Korea

  • Lee, Jun Won;Park, Myung Soo;Park, Ji-Hyun;Cho, Yoonhee;Kim, Changmu;Kim, Chang Sun;Jo, Jong Won;Lim, Young Woon
    • Mycobiology
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    • 제48권6호
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    • pp.476-483
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    • 2020
  • The genus Pholiota (Strophariaceae, Basidiomycota) is made up of wood-rotting saprotrophic mushrooms characterized by a yellow or brown pileus with scales and/or slimy, and by a brownish smooth spore with a germ pore. However, these features are not enough to distinguish its species, or separate the genus Pholiota from other brown-spored wood-rotting genera such as Hypholoma and Stropharia. Although internal transcribed spacer (ITS) sequencebased identification has improved identification accuracy for species of Pholiota, most Pholiota species in Korea are reported based on morphological features. To evaluate the taxonomy of Pholiota species, we investigated 62 specimens collected from 1999 to 2019 in Korea using ITS sequence analysis and morphological observation. Twelve of the 16 recorded Pholiota species in Korea were identified. While eight species were clearly separated, the ITS analysis did not distinguish three in the Pholiota adiposa complex. Therefore, further investigation is required to distinguish these three species. ITS sequences deposited in GenBank confirm that P. highlandensis exists in Korea. The presence of the other four Pholiota species could not be confirmed through specimens or sequence information in GenBank. A taxonomic key and the ITS sequence data for Korean Pholiota species are included and can be good baselines for further research on Pholiota taxonomy and diversity.

Diversity, Interspecific Interaction and Abundance of Undergrowth in Monocultures and Integrated Systems of Natural Rubber Plantation in Danzhou, Southern China

  • Chima, Uzoma Darlington;Qi, Dongling;Wu, Zhixiang;Lan, Guoyu;Chen, Li
    • Journal of Forest and Environmental Science
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    • 제38권2호
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    • pp.75-89
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    • 2022
  • The negative impact of monoculture rubber plantations on biodiversity and associated ecological processes/ecosystem services has led to suggestions on the use of integrated land use systems for rubber cultivation and production in order to ensure environmental sustainability. However, there is paucity of information on the effect of such integrated land use systems on the diversity and abundance of the rubber plantation undergrowth. We evaluated and compared undergrowth plant species composition, richness, abundance, diversity and interaction, in three integrated systems (Rubber-Strelitzia reginae Integrated System - RSrIS, Rubber-Podocarpus nagi Integrated System - RPnIS & Naturally Managed Rubber Plantation - NMRP) with three Rubber Monoculture Plantations (RMP1, RMP2 & RMP3) adjacent to the integrated systems, respectively, at the Investigation and Experiment Station of Tropical Crops, Danzhou, Hainan, China. Undergrowth species density was higher in the rubber monocultures than in the integrated systems except in RSrIS. Species richness and diversity were also higher in the monocultures except in NMRP. Species similarity/interaction between the monocultures and the integrated systems was highest between RMP3 and NMRP. The NRMP proved to be the best model of natural rubber integrated system for the conservation of undergrowth species richness, diversity and interspecific interaction. However, the conservation of undergrowth species in other forms of integrated natural systems can be enhanced by considering the ecology of species to be integrated in terms of their growth characteristics, competitive nature, and ability to grow in association with other species.

The diet of three commercial fishes based on stomach contents in the Yellow Sea

  • Heeyong Kim;Wongyu Park;Jung Hwa Choi
    • Fisheries and Aquatic Sciences
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    • 제26권10호
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    • pp.628-636
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    • 2023
  • Stomach contents of three commercially important species, anchovy (Engraulis japonica), small yellow croaker (Larimichthys polyactis) and yellow goosefish (Lophius litulon) were analyzed to investigate difference of prey between Spring and Fall. Trawl surveys for target fishes were conducted at 12 stations by the RV Tamgu-8 in the Yellow Sea-Korean side in Spring and Fall 2008 as a part of the United Nations Development Programme/Global Environment Facility (UNDP/GEF), Yellow Sea Large Marine Ecosystem (YSLME) survey. Stomach contents of 50 individuals of each species were analyzed to species level of prey, if the number of specimens was more than 50 for each species. Fullness and digestion condition of stomach contents were determined by five and six levels, respectively. In anchovy stomachs, 23 species in Spring and 15 species in Fall were identified, respectively. Stomach contents were mostly occupied by copepods and euphausiids, mostly Euphausia spp., calyptopis in Spring while by copepods and amphipods in Fall. In small yellow croaker stomachs, 23 species in Spring and 11 species in Fall were identified. Stomach contents were mostly occupied by copepods and euphausiids in Spring, but by only euphausiids in Fall. Total 368 yellow goosefish (151 in Spring and 217 in Fall) were captured, but stomach contents only in Fall were analyzed. Most of stomach contents were anchovy with small proportion of Hakodate sand shrimp, Tanaka's snailfish, Pacific cod, and miscellaneous things. The present research unveiled that main food items of plankton feeder were distinctly different by species and seasons in the Yellow Sea-Korean side, as coincided with previous reports.

오륙도 주변해역 저서 갑각류 (Crustacea)의 종조성과 군집구조 (Species composition and community structure of benthic crustacean assemblage around Oryukdo in southeastern waters off Korea)

  • 허유심;최정화;김정윤;이동우
    • 수산해양기술연구
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    • 제50권4호
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    • pp.604-613
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    • 2014
  • Community structures and species composition in benthic crustacean around Oryukdo of Busan was investigated using a SCUBA diving in 2011. A total 115 species, 51 families, and 4 taxa were collected during the sampling periods. Species were included 52 species in amphipoda, 49 in decapoda, 8 in isopoda and 6 in thoracica. On cluster analysis, the benthic crustacea community was classified into three groups based on the species composition: group A occurred in spring, group B in autumn and winter and group C in summer. Species composition in benthic crustacean corresponded with the water temperature.

A taxonomic review of the genus Acteniceromorphus Kishii, 1955 (Coleoptera; Elateridae) in Korea

  • Han, Taeman;Park, In Gyun;Park, Haechul
    • International Journal of Industrial Entomology and Biomaterials
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    • 제31권2호
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    • pp.40-47
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    • 2015
  • The genus Acteniceromorphus is taxonomically reviewed for the first time in Korea. From the previously recorded three species, two species, A. selectus (Candèze, 1894) and A. fulvipennis (Lewis, 1894) are confirmed as misidientification of species which are endemic to Japan. Another species, A. chlamydatus (Lewis, 1894) is unavailable any Korean specimens. Additionally, we found A. ferrugineipennis (Candèze, 1879) as new to Korea. We also provide a comparison of DNA barcoding for two species previously misidentified and the newly recorded species, except A. chlamydatus. DNA barcoding result shows that each species is clearly delimited at species-level from each other by large interspecific genetic distance over 7.2%.

Taxonomic study on bryozoans - new additions to the Korean fauna and new species of Petraliella from Seogwipo waters of Jeju Island

  • Chae, Hyun Sook;Kil, Hyun Jong;Seo, Ji Eun
    • Journal of Species Research
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    • 제5권3호
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    • pp.551-565
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    • 2016
  • This study aims to clarify the diversity of bryozoans which were collected from Seogwipo waters in Jeju Island, Korea. A total of 78 species has been reported from Seogwipo waters so far. In the present study, 63 species were found from nine sites of Seogwipo waters from 2008 to 2012, including six newly added species to the Korean bryozoan fauna and twelve species new to Seogwipo waters. As a result of this study, the bryozoans from Seogwipo waters were turned out to be 96 species, 64 genera, 42 families, three orders and two classes.

금병산 초본군집의 지하부 구조 : 영양생장식물의 우점도에 관하여 (Radicoid Structure of Herbaceous communities in Mt. Kumbyung : Special Emphasis on the Dominance of Clonal Plants)

  • 정연숙;이규송
    • The Korean Journal of Ecology
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    • 제21권3호
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    • pp.243-249
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    • 1998
  • Radicoid structure of herbaceous communities in Mt. Kumbyung, Chunchon, Kangwon-Do was clarified emphasizing on the dominance of clonal plants in relation to vegetation development. In the total vegetation, 46% species identified were rhizomatous, 26% were species with tap roots, and 13% were stoloniferous species. Thus, all clonal species, including plants with bulb, corm and tuber, covered 62.8% of the total species. in the understory of Quercus, Pinus and Larix forests, 68.2% were clonal, which means 5% more compare to total vegetaion. Dominance of clonal plants became more conspicuous as their importance value was calculated. That is, they covered average 86% for the following three communities: 85% in Quercus, 95% in Pinus, and 76% in Larix, Especially, the rhizomatous plants were remarkable. Overall, clonal species dominated over the other radicoid types in terms of not only the number of species, but also the importance value. Dominance of these species was more peculiar in understory of forests, indicating the occupancy of clonal plants was related to the vegetation development.

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