• Title/Summary/Keyword: thermal stabilizers

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Stabilization of Dry Immuno-Diagnostic Kit with Immobilized Antibody and Determination of Its Shelf-Life (항체가 건조상태로 고정된 면역진단키트의 안정화와 Shelf-Life 결정)

  • 이창우;조정환;육순학;권오협;박영남;박세환
    • KSBB Journal
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    • v.13 no.5
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    • pp.502-510
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    • 1998
  • Stabilization of antibody, which is specific to Salmonella typhimurium antigens, present in dry states on membranes was accomplished, and its shelf-life, i.e., duration for maintaining minimum 90% of the initial activity, under optimal conditions was determined. To prepare two major components of an immuno-strip, the antibody was not only immobilized on nitrocellulose membrane surfaces but also placed within the pores of glass fiber membrane after conjugating it with old colloids as signal generator. Among potential stabilizers of the immuno-components, a disaccharide, trehalose, showed a significant protection effect of immunoglobulin structure from thermal energy. Optimal concentrations of trehalose for the respective component were significantly different (8-fold higher for the antibody-gold conjugate than for the immobilized antibody), which probably resulted from distinct densities and configurations of antibody present on the membranes. An additional requirement for the gold conjugate was freeze-drying of this substance such that the conjugate can be readily resolubilized upon contact with aqueous medium. By using the components prepared under optimal conditions, immuno-strips were constructed and exposed to thermal energy. Signals with less than 10% decrease in the intensity were maintained for approximately 21 days at 60$^{\circ}C$. Compared to previous reports, this result represented a 2-year shelf-life at room temperature. it was, however, two times longer if determined from thermal acceleration tests based on the theory of inactivation rate of protein. Such discrepancy between the two estimates could be mainly attributed to errors in accurately controlling temperatures and also to changes in the physical properties of membranes due to a high thermal energy.

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Effect of La and Si Addition on Thermal Stabilization of Alumina (La 및 Si 첨가에 의한 알루미나의 열안정화 효과)

  • Lee, Chae-Hyun;Lim, Dae-Young;Kim, Jong-Ock;Seo, Doo-Won;Han, Moon-Hee
    • The Journal of Engineering Research
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    • v.3 no.1
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    • pp.215-222
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    • 1998
  • The effect of La and Si addition of the thermal stabilization of $\gamma-alumina$ powers have been studied. Reagent grade $La(NO_3)_3{\cdot}6H_2O$ and $Si(OC_2H_5)_4$ were used as starting materials. These additives were introduced by wet impregnation method. Both La and Si additives suppressed the sintering of alumina and were found to be good thermal stabilizers of $\gamma-alumina$. Especially, Si drastically suppressed the phase transition of alumina at high temperatures. The major mechanisms for the thermal stabilization of alumina were seemed to be new phase formation and retardation of surface diffusion by addition of La or Si into alumina matrix.

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Fuzzy Applications in a Multi-Machine Power System Stabilizer

  • Sambariya, D.K.;Gupta, Rajeev
    • Journal of Electrical Engineering and Technology
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    • v.5 no.3
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    • pp.503-510
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    • 2010
  • This paper proposes the use of fuzzy applications to a 4-machine and 10-bus system to check stability in open conditions. Fuzzy controllers and the excitation of a synchronous generator are added. Power system stabilizers (PSSs) are added to the excitation system to enhance damping during low frequency oscillations. A fuzzy logic power system stabilizer (PSS) for stability enhancement of a multi-machine power system is also presented. To attain stability enhancement, speed deviation ($\Delta\omega$) and acceleration ($\Delta\varpi$) of the Kota Thermal synchronous generator rotor are taken as inputs to the fuzzy logic controller. These variables have significant effects on the damping of generator shaft mechanical oscillations. The stabilizing signals are computed using fuzzy membership functions that are dependent on these variables. The performance of the fuzzy logic PSS is compared with the open power system, after which the simulations are tested under different operating conditions and changes in reference voltage. The simulation results are quite encouraging and satisfactory. Similarly, the system is tested for the different defuzzification methods, and based on the results, the centroid method elicits the best possible system response.

Anthocyanin-Contents and Pigment Stability of Black Soybean by Different Extract Condition and Stabilizer (추출조건과 첨가물에 따른 검정콩의 안토시아닌 함량과 색소 안정성)

  • Lee, Hye-Jeong;Choi, Eun-Young;Sim, Young-Ja;Kim, Ok-Sun;Yoo, Ho-Jung;Do, Wan-Nyeo;Kim, Yong-Ho
    • The Korean Journal of Food And Nutrition
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    • v.22 no.1
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    • pp.150-157
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    • 2009
  • The purpose of this study was to analyze the anthocyanin contents of black soybean crude extracts derived using a countercurrent system and to compare the effects of stabilizers(${\beta}-cyclodextrin$, maltodextrin) and sugars(sucrose, maltose) on the color deterioration of the anthocyanin. When the extraction process was kept at 100$^{\circ}C$ for 120$\sim$180 min, only C3G (cyanidin-3-glucoside) was detected in the water extract. The C3G contents in the water extracts acquired at 8$^{\circ}C$, 60$^{\circ}C$, and 80$^{\circ}C$ were 2.38 ppm, 1.73 ppm, and 1.73 ppm, respectively. Sucrose and maltose retarded color deterioration of the crude pigment extract by the countercurrent method with methanol. Finally, the additions of maltodextrin or ${\beta}-cyclodextrin$ did not retard thermal color deterioration of the black soybean crude pigment extract.

Effect of Phenolic Antioxidants System on Yellowing of Amorphous Poly-α-olefin (페놀계 산화방지제에 의한 비결정성 올레핀 수지의 황변 거동)

  • Kim, Si-Yong;Kim, Ho-Gyum;Park, Sang-Cheol;Min, Kyung-Eun
    • Polymer(Korea)
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    • v.37 no.2
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    • pp.156-161
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    • 2013
  • Phenolic antioxidants are effective stabilizers that provide excellent long-term heat stability by preventing thermo-oxidative degradation during processing and service life. However, under a selected set of circumstances, certain types of phenolics have been susceptible to discoloration due to prolonged storage in an environment containing oxides of nitrogen. It is investigated that the effect of addition of secondary antioxidant and chemical structure of primary antioxidant on discoloration of amorphous poly-${\alpha}$-olefin (APAO), which is especially prone to be decomposed in high processing temperature. From the result, it is concluded that a higher level of steric hindrance of phenolic antioxidant provided by long alkyl chain allows a more enhanced synergic effect with secondary antioxidant.

Fabrication of Thin Film Transistors based on Sol-Gel Derived Oxide Semiconductor Layers by Ink-Jet Printing Technology

  • Mun, Ju-Ho;Kim, Dong-Jo;Song, Geun-Gyu;Jeong, Yeong-Min;Gu, Chang-Yeong
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.05a
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    • pp.16.1-16.1
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    • 2009
  • We have fabricated solution processed oxide semiconductor active layer for thin film transistors (TFTs). The oxide semiconductor layers were prepared by ink-jet printing the sol-gel precursor solution based on doped-ZnO. Inorganic ZnO-based thin films have drawn significant attention as an active channel layer for TFTs applications alternative to conventional Si-based materials and organic semiconducting materials, due to their wide energy band gap, optical transparency, high mobility, and better stability. However, in spite of such excellent device performances, the fabrication methods of ZnO related oxide active layer involve high cost vacuum processes such as sputtering and pulsed laser deposition. Herein we introduced the ink-jet printing technology to prepare the active layers of oxide semiconductor. Stable sol-gel precursor solutions were obtained by controlling the composition of precursor as well as solvents and stabilizers, and their influences on electrical performance of the transistors were demonstrated by measuring electrical parameters such as off-current, on-current, mobility, and threshold voltage. Microstructure and thermal behavior of the doped ZnO films were investigated by SEM, XRD, and TG/DTA. Furthermore, we studied the influence of the ink-jet printing conditions such as substrate temperature and surface treatment on the microstructure of the ink-jet printed active layers and electrical performance. The mobility value of the device with optimized condition was about 0.1-1.0 $cm^2/Vs$ and the on/off current ratio was about $10^6$. Our investigations demonstrate the feasibility of the ink-jet printed oxide TFTs toward successful application to cost-effective and mass-producible displays.

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