• 약학회지
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• 제57권4호
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• pp.265-271
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• 2013

Treatments of vascular disease via modulating the expression of specific proteins by gene transfer have been attempted in various studies over the past few years. Among several methods to deliver genes, adenovirus currently has been used because of a number of positive aspects. In this study, we test adenoviral vector as a potential mediator in the treatment of vascular disease by using freshly isolated vascular tissues not cultured vascular cells. Freshly isolated vascular tissues were directly exposed to adenoviral vector pAd5CMVmcsIRESeGFPpA to check the possibility of GFP expression in different layer of vascular tissues. We found that the GFP expression by using adenoviral vector experiments is mainly focused on the adventitia and failed to detect GFP expression at endothelial layer or vascular smooth muscle layer in vascular tissues. However, we also found that several integrin receptors are robustly expressed in vascular smooth muscle, thus the limited expression of protein in vascular smooth muscle are not likely the lack of integrin receptors. In conclusion, adenovirus could not be a good tool for a specific protein expression in vascular smooth muscle cell. Thus, the application of adenovirus as a tool for gene therapy of vascular smooth muscle cells in clinical therapeutic trial need to be optimized further.

• Parasites, Hosts and Diseases
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• 제59권3호
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• pp.257-263
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• 2021

Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.

• Tuberculosis and Respiratory Diseases
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• 제85권4호
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• pp.302-312
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• 2022

Chronic obstructive pulmonary disease (COPD) is a complex and heterogeneous disease. Not all patients with COPD respond to available drugs. Identifying respondents to therapy is critical to delivering the most appropriate treatment and avoiding unnecessary medication. Recognition of individual patients' dominant characteristics by phenotype is a useful tool to better understand their disease and tailor treatment accordingly. To look for a suitable phenotype, it is important to understand what makes COPD complex and heterogeneous. The pathology of COPD includes small airway disease and/or emphysema. Thus, COPD is not a single disease entity. In addition, there are two types (panlobular and centrilobular) of emphysema in COPD. The coexistence of different pathological subtypes could be the reason for the complexity and heterogeneity of COPD. Thus, it is necessary to look for the phenotype based on the difference in the underlying pathology. Review of the literature has shown that clinical manifestation and therapeutic response to pharmacological therapy are different depending on the presence of computed tomography-defined airway wall thickening in COPD patients. Defining the phenotype of COPD based on the underlying pathology is encouraging as most clinical manifestations can be distinguished by the presence of increased airway wall thickness. Pharmacological therapy has shown significant effect on COPD with airway wall thickening. However, it has limited use in COPD without an airway disease. The phenotype of COPD based on the underlying pathology can be a useful tool to better understand the disease and adjust treatment accordingly.

• Radiation Oncology Journal
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• 제11권2호
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• pp.267-275
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• 1993

1985년 3월부터 1990년 9월까지 구강암으로 진단받고 본원 치료방사선과에서 방사선치료를 시행하였던 85명의 환자에 대한 치료결과를 후향적으로 분석하였다. 이중 방사선 단독치료가 37예였고 수술후 방사선치료가 48예였다. 방사선치료 방법으로는 70예에서 $^{60}Co$와 전자선에 의한 외부방사선조사로만 시행하였고 조직내삽입 복합요법이 7예, oral cone추가사용이 8예였다. 전체환자중 설암이 40예로 가장 많았고 구강저부암이 17예, 구개암이 12예, 후구치삼각부암을 포함한 치육암이 10예, 구협암이 5예, 구순암이 1예였다. 병리소견상 편평상피세포암이 77예로 가장 많았으며, AJC TNM 병기에 의한 병기 I+II기는 28예였고 병기 III+IV기 환자의 3년생존율은 각각 $60.9\%$와 $23.1\%$였다. 예후인자에 대한 분석상 원발병소의 크기가 유의하였다(P<0.01). 기타 나이, 원발병소의 위치, 림프절 전이여부, 치료방법(수술여부), 조사선량, 종양세포의 조직학적 등급 등에 의한 생존율과 국소제어율의 차이는 통계학적으로 유의한 결과를 얻을 수 없었다. 이중 치료방법에 따르면, 원발병소의 크기가 클수록, 혹은 림프절 전이여부를 막론하고 수술과 방사선 병용치료군에서 방사선 단독치료군보다 유의하지는 않았지만 더 높은 생존율을 보였다(p<0.1). 결론적으로 조기구강암에서는 방사선 단독치료가 수술과 방사선 병용치료에 비해 비슷한 치료성적을 보이면서도 특히 해부학적, 기능적 장애를 야기하는 수술에 비해 더 효과적이며 진행성구강암에서는 수술과 방사선 병용치료가 적절하다고 사료된다.반응율은 높으나 화학요법 및 방사선치료가 국소관해율 및 생존율의 향상으로는 연결되지는 않았다. 결론적으로 진행된 비인강암에서의 화학요법은 좀더많은 비교대조군 연구(controlled clinical trial)를 통해서만 역 할을 이야기할 수 있을 것으로 사료된다. performance status(KPS), 침습부위, 수술적 제거여부 및 제거정도, 방사선치료선량, 방사선조사야, 화학요법 병행 여부에 따라 생존률을 분석한 결과 연령 (p=0.0121), KPS(p=0.0002), 조직학적 등급(P=0.0001), 수술적 제거 (p=0.0240)가 유의한 예후인자로 분석되었으며, 통계학적으로 유의하지는 않았지만 천막하병소가 천막상부 병소에 비해, 부분조사가 전뇌조사에 비해 높은 생존률을 보이는 매개변수로 분석되었다.련된 생존율에 영향을 주었던 인자로는 나이 (p<0.0291), 병기(p<0.0001), 전신상태(p<0.0041), 초기 혈색소 수치 (p<0.0001), 강내 조사(p<0.0004)였고, 조직학적 소견(p<0.29), 유도 화학요법과의 병행치료(p<0.87)는 통계학적으로 유의하지 않았다..0093{\pm}0.0047)\;D^2+(13.31{\pm}7.309$) 였었다. 감마선에 대한 중성자선의 상대적 생물학적 효과비 (RBE)는 y=aD+$bD^2$+c를 다음과 같은 식으로 변형시켜 계산하였다. $$\frac{[-a{pm}\sqrt{a^2-4b\;(c-y}}]}{2{\times}6}$$ 미세핵 발생빈도가 세포당 0.05와 0.8사이에서의 중성자선의 상대적 생물학적 효과비는 $2.37{\pm}0.

• 대한정형도수물리치료학회지
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• 제17권1호
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• pp.35-44
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• 2011

Background: This study was to suggest the process of making strategy for effective intervention and evaluation on functional problems of the frozen shoulder patient applied International Classification of Functioning Disability and Health (ICF) Tools. Methods: The patient was 48years old woman with right shoulder adhesive capsulitis and calcific tendinitis. In first phase, therapists could list the information relating to functional problems used by the ICF Core set and then could confirm the interaction among the problems using the ICF assessment sheet. In second phase, therapist is needed to make the hypothesis and hypothesis testing and then set a primary functional goals and therapeutic goals in detail after prioritizing the problems to be managed based on the problem list. Lastly, after setting the confirmed problems as the purpose of intervention through the hypothesis testing, therapist could do some intervention after making a plan to solve these problems. Results: This report illustrates how to apply the process based on ICF concept into physical therapy practice. The decided hypothesis and goal that are to solve the problems the client faced were remarkably meaningful. Conclusions: Clinical decision making for the most effective intervention requires that therapists use the clinical reasoning process based on ICF concept.

• Nutrition Research and Practice
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• 제9권3호
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• pp.268-277
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• 2015

BACKGROUND/OBJECTIVES: Although it is crucial to identify those children likely to be treated in an appropriate nutrition rehabilitation programme and discharge them at the appropriate time, there is no golden standard for such identification. The current study examined the appropriateness of using Mid-Upper Arm Circumference for the identification, follow-up and discharge of malnourished children. We also assessed its discrepancy with the Weight-for-Height based diagnosis, the rate of recovery, and the discharge criteria of the children during nutrition rehabilitation. SUBJECTS/METHODS: The study present findings from 156 children (aged 6-59 months) attending a supplementary feeding programme at Makadara and Jericho Health Centres, Eastern District of Nairobi, Kenya. Records of age, weight, height and mid-upper arm circumference were selected at three stages of nutrition rehabilitation: admission, follow-up and discharge. The values obtained were then used to calculate z-scores as defined by WHO Anthro while estimating different diagnostic indices. RESULTS: Mid-upper arm circumference single cut-off (< 12.5 cm) was found to exhibit high values of sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, negative likelihood ratio at both admission and discharge. Besides, children recorded higher rate of recovery at 86 days, an average increment of 0.98 cm at the rate of 0.14mm/day, and a weight gain of 13.49gm/day, albeit higher in female than their male counterparts. Nevertheless, children admitted on basis of low MUAC had a significantly higher MUAC gain than WH at 0.19mm/day and 0.13mm/day respectively. CONCLUSIONS: Mid-upper arm circumference can be an appropriate tool for identifying malnourished children for admission to nutrition rehabilitation programs. Our results confirm the appropriateness of this tool for monitoring recovery trends and discharging the children thereafter. In principle the tool has potential to minimize nutrition rehabilitation costs, particularly in community therapeutic centres in developing countries.

• 대한물리치료과학회지
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• 제18권1호
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• pp.33-49
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• 2011

The process of physical therapy uses a problem-solving approach to enhance a patients's functioning status. The International Classification of Functioning, Disability and Health(ICF) is the common concept for the functioning in the world. Physical therapists require the ability to identify problems, formulate hypothesis, and plan intervention strategies through clinical reasoning. In the clinical process, physical therapists need to use standard and common languages in speech and in documentation. The purpose of this study was to suggest the process of making strategy for efficient intervention, examining and evaluating the functional problem of the person with stroke using ICF tools. For the first step in this process model, therapists could list the information relating to functional problems used by the ICF Core set and then could identify the interaction among the problems using the ICF assessment sheet. For the next step, therapist is needed to make the hypothesis and hypothesis testing, and then set a primary functional goals and therapeutic goals in detail after prioritizing the problems to be managed based on the problem list. Finally, after setting the identified problems as the purpose of intervention through the hypothesis testing, therapist could do some intervention after making a plan to solve these problems, and find out the outcomes using the ICF evaluation display. This report illustrates how to apply the process based on ICF concept into physical therapy practice. Making a decision for the most efficient intervention requires that therapists use the clinical reasoning process based on ICF concept.

• Physical Therapy Rehabilitation Science
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• 제8권2호
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• pp.99-110
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• 2019

Objective: The aim of the present study was to develop a valid and reliable scale that measures the healthy life styles among young adults. Design: A methodological study design was employed to develop and validate the Healthy Lifestyle Screening Tool (HLST). Methods: The validity and reliability of the HLST were established in accordance with DeVellis' 8 steps guideline for tool development. The question items were generated based on literature reviews and interviews, which were then classified into 12 categories. The HLST was administered to 272 students attending a Korean university. The reliability was tested using Cronbach's alpha. The validity of the scale was examined with the mean inter-item correlations (MIIC) and factor analysis, and was also examined for content validity by experts. Results: The reliability of the HLST was found to be acceptable, as indicated by a Cronbach's alpha of 0.71. In the validity test, items with less than 80% "agreement" ratings on the content validity index by experts were revised. The MIIC values were greater than 0.25. A factor analysis of 36 items extracted 9 factors (i.e., four items per factor), which together explained 50.4% of the variance. The HLST consists of 36 items that measure 9 factors based on a 4-point Likert rating scale, with 4 items per factor, as follows: sunlight, water, air, rest, exercise, nutrition, temperance, trust, and general physical condition. High scores on the HLST are indicative of a healthy lifestyle (HL). Conclusions: The HLST is a valid and reliable scale that can be used to measure HL among young adults. Identification of HL by using the HLST can provide guidance to integrated therapeutic approaches along with conventional physical therapy.

• 재활치료과학
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• 제9권3호
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• pp.53-61
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• 2020

목적 : 본 연구는 운전능력 평가도구를 이용하여 운전과 관련된 반응속도와 분리집중력을 평가하여, 고령자의 연령대에 따른 평균점수의 차이 분석으로 추후 고령자의 운전위험여부 진단을 위한 기초 자료를 제공하고자 한다. 연구방법 : 본 연구의 대상은 2019년 8월부터 12월까지 강원도 W시에 거주하고 있는 65세 이상 복지관 이용 노인 70명을 대상으로 운전 평가 프로그램을 실시하였다. 1차적으로 운전관련 설문지를 작성한 후, 운전평가도구에 대한 설명을 듣고 반응속도와 분리집중력과제에 대한 평가를 진행하였다. 본 연구의 수집된 자료는 통계프로그램 SPSS 25.0을 이용하여 분석하였고, 유의수준은 0.1로 설정하였다. 결과 : 전기노인의 반응속도 과제에 대한 반응시간 평균은 0.717초, 후기노인은 0.843초로 유의수준 0.1에서 유의미한 차이가 나타났다(p=.084). 분리집중력 과제에 대한 반응시간은 0.669, 후기노인은 0.695로 유의미한 차이가 없었다(p=.901). 결론 : 운전능력 평가도구를 이용하여 고령운전자의 운전 시 반응속도와 분리집중력을 평가할 수 있었고, 향후 운전 평가도구 개발의 기초 자료로 활용될 수 있을 것이다.

• 한국산학기술학회논문지
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• 제21권4호
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• pp.173-180
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• 2020

본 연구는 기본간호학실습 교육에서 핵심기본간호술 수행 시 의사소통능력 평가에 사용할 수 있는 평가도구를 구성하고 적용 결과를 기술한 사례연구이다. 문헌고찰과 전문가 검토를 통해 의사소통능력 평가도구를 구성하였고, 2019년 10월부터 11월까지 일 대학 기본간호학실습 교과목을 수강 중인 94명을 대상으로 적용 후 결과를 분석하였다. 연구결과, 핵심기본간호술 수행 시 평가되어야 할 의사소통능력 항목으로 5가지 요인(자기 소개, 눈맞춤, 정서적 지지, 정보제공, 치료적 접촉)이 도출되었으며 활력징후를 측정하는 기본간호술 수행 시 평가된 의사소통능력은 5점 만점에 평균 3.96점으로 나타났다. 루브릭에 따른 학습성과 달성도는 전체 학생의 95.8%가 '중' 이상에 도달하여 목표 성취수준에 도달한 것으로 나타났다. 본 연구는 간호대학생의 졸업 시 역량으로 핵심기본간호술과 의사소통능력이 요구되는 현실에서, 기본간호학실습 교육과정에서 사용 가능한 의사소통능력 평가도구를 구성하고 적용하여 학습성과 달성도를 확인함으로써 학습성과 평가과정을 개선하고 체계적인 평가 체계를 구성하는데 중요한 근거를 제공하는 의의가 있다. 향후 본 연구에서 도출된 의사소통능력 평가도구의 타당성과 신뢰도 확보를 위한 연구, 다양한 평가도구와의 비교 연구를 제언하는 바이다.