• Parasites, Hosts and Diseases
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• v.56 no.2
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• pp.105-112
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• 2018

Blastocystis is an enteric Straminopile in tropical, subtropical and developing countries. Metronidazole has been a chemotheraputic for blastocystosis. Failures in its regimens were reported and necessitate new studies searching for alternative therapeutic agents. Aim of current study is to investigate potential effects of Atorvastatin (AVA) compared to the conventional chemotherapeutic MTZ in experimentally Blastocystis-infected mice. Anti-Blastocystis efficacy of AVA was evaluated parasitologically, histopathologically and by transmission electron microscopy using MTZ (10 mg/kg) as a control. Therapeutic efficacy of AVA were apparently dose-dependent. Regimens of AVA (20 and 40 mg/kg) proved effective against Blastocystis infections with highreduction in Blastocystis shedding (93.4-97.9%) compared to MTZ (79.3%). The highest reductions (98.1% and 99.4%)were recorded in groups of combination treatments AVA 20-40 mg/kg and MTZ 10 mg/kg. Blastocystis was nearly eradicated by the 20th day post infection. Genotype analysis revealed that genotype I was most susceptible, genotype III was less. Histopathologic and ultrastructural studies revealed apoptotic changes in Blastocystis and significant improvement of intestinal histopathological changes more remarkable in combinational therapy groups. Thus, the present study offers AVA as a potential candidate for Blastocystis therapy combined with MTZ.

• Journal of Veterinary Clinics
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• v.11 no.1
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• pp.401-405
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• 1994

This research was carried out to determine the effect of various therapies on the hyperthermic symptom of the Holstein cattle, The 50 cases were calassified as 3 groups according to the therapies aplied, needle Acupuncture therapy, antipyretic administration therapy, combination of prior 2 therapies. The results are as follows. 1. On the 23 cases applied needle acupuncture therapy only, the body temperature was decreased from 39.9$\pm$0.7$^{\circ}C$ to 38.6$\pm$0.3$^{\circ}C$ after 4 hours from application. It was in the arrange of the normal body temperature. 2. On the 10 cases administered antipyretic drug, the body temperature was decreased from. 39.8$\pm$0.3$^{\circ}C$ to 38.7$\pm$0.1$^{\circ}C$ after 4 hours from application. the therapeutic efficacy of these cases found somewhat inferiopr to the cases acupunctured. 3. On the 9 cases comined both acupuncture and antipyretic administration therapy, the body temperature was decreased from 40.1$\pm$0.4$^{\circ}C$ to 38.5$\pm$0.1$^{\circ}C$ after 4 hours from application. The therapeutic efficacy found the best among the 3 groups.

• Journal of Veterinary Clinics
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• v.19 no.2
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• pp.115-120
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• 2002

To clarify the therapeutic effect of aquapuncture therapy on bovine foot rot, the experimental cattle were divided into control (conventional treatment), aquapuncture and combined treatment groups. Each group was composed of 6 Holstein cows with bovine foot rot. In the present study, the change in grade of lameness, total leukocyte counts, N/L. ratio, mean recovery rates (days) and histopathological changes of interdigital tissue before and after treatment were evaluated. The grade of lameness was decreased by treatment in each group, with the decreasing in order of combined > aquapuncture > control group. In change of total peripheral blood leukocyte counts, the tendency of decrease was found. Significances were detected on 9th day (P < 0.05) in control, on 6th day (P < 0.01) in aquapuncture group, and on 3rd (P < 0.05) and 6th day (P < 0.05) in combined group, respectively. In addition, significances were detected on 3rd and 6th day in aquapuncture, and on 3rd day in combined group by comparison with that of control, respectively. N/L ratio was decreased by therapy in each group with the decreasing in order of combined > aquapuncture ) control group. Significances were detected on 6th day (P < 0.01) between aquapuncture and combined groups. The mean recovery rates(days) were rapid with the decreasing in order of combined > acuapuncture > control group. In histopathological findings of interdigital tissue, the findings of inflammation and hemorrhage were not observed after treatment in each group. In conclusion, it was thought that aquapuncture and combined therapy was very effective on treatment of bovine foot rot.

• Journal of Pharmacopuncture
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• v.26 no.4
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• pp.357-365
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• 2023

Objectives: Since stroke is a serious health issue, novel therapeutic strategies are required. In a mouse model of ischemic stroke, this study analyzed the potential of electroacupuncture (EA) and tenuigenin (TE) to improve the efficacy of human mesenchymal stem cell (hMSC) transplantation. Methods: Middle cerebral artery occlusion (MCAO) with reperfusion was used to generate ischemic stroke. Forty-eight male C57BL/6 mice were randomly divided into five groups: control, MCAO-operated, MCAO-EA, MCAO-TE, or MCAO + EA + TE. Subsequently, hMSCs were transplanted into the ischemic region and EA, TE, or the combination was administered. Behavior assessments and immunohistochemistry were conducted to evaluate motor and cognitive recovery and hMSCs survival, migration, and differentiation. Results: The combined treatment of EA and TE exhibited enhanced hMSCs survival, migration and differentiation into neural cell lineages while suppressing astrocyte formation. Immunohistochemistry demonstrated increased neurogenesis through hMSCs transplantation in the ischemic brain. Immediate behavioral improvements were not significantly different between groups, but there was a gradual recovery in motor and cognitive function over time. Conclusion: These findings highlight the potential of EA and TE co-treatment as a therapeutic strategy for ischemic stroke, opening avenues for further research to optimize treatment protocols and elucidate underlying mechanisms.

• Journal of Veterinary Clinics
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• v.30 no.1
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• pp.17-21
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• 2013

The objectives in the present study were to evaluate the therapeutic effect of a sodium salts mixture against Salmonella gallinarum infection in broiler. Of this study, sixty broilers at two weeks of age (body weight, $450{\pm}35$ g) were used to estimate the efficacy of a sodium salts mixture (3.25 mg sodium azide, 2.45 mg sodium cyanide, 0.8 g sodium chlorate) against Salmonella gallinarum infection in broiler with drinking water. Broilers challenged with S. gallinarum were administered with ${\times}$ 1(group I) and ${\times}$ 2(group II) sodium salt mixture for seven days, and cecal content samples were collected at the gate of treatment and on 1st, 3rd, 5th, and 7th day after administration. Changes in body weight and cecal shedding of S. gallinarum were monitored during the experimental period. All groups treated with the sodium salt mixture slightly increased body weight compared to control group but there is no significant difference. At 7th day after administration, the number of S. gallinarum in group I and II was significantly decreased compared to control group (p < 0.001). In the hematological and blood biochemical analysis, values of parameters were not significantly different between the treated groups and control group. From results of the present study, the sodium salt mixture had therapeutic effect on S. gallinarum infection in broilers.

• Journal of Veterinary Clinics
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• v.25 no.4
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• pp.245-248
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• 2008

Herbal medicines are widely used to treat a variety of human diseases. However, therapeutic effect of herbal medicine on the canine facial nerve paralysis (FNP) has not been investigated. This study examined therapeutic effects of Oyaksungisan on the canine FNP. Ten dogs with the induced canine FNP were equally divided into the control and an herbal medicine-treated groups (5 dogs, Oyaksungisan-treated group), respectively. No treatment was given to the control group. In the Oyaksungisan-treated group, Oyaksungisan was administered orally, twice per day for 2 weeks (50 mg/kg) after inducing FNP. Significant differences in the clinical scores were detected between the Oyaksungisan-treated and control groups on day 14 (p < 0.05). The serum creatine kinase (CK) activities in the Oyaksungisan-treated group showed a tendency to decrease but there was no significant difference compared with those of the control group. In conclusion, Oyaksungisan was effective for the induced canine FNP.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.647-650
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• 2014

Objective: To explore the efficiency of single application of lobaplatin in tran-scatheter arterial chemoembolization (TACE) for patients with a primary hepatic carcinoma who were unable or unwilling to undergo surgery. Methods: 173 patients with primary hepatic carcinoma diagnosed by imaging or pathology were randomly divided into experimental and control groups and respectively treated with lobaplatin and pirarubicin hydrochloride as chemotherapeutic drugs for TACE. The amount of iodipin was regulated according to the tumor number and size, and then gelatin sponge or polyvinyl alcohol particles were applied for embolisms. The efficiency of treatment in the two groups was compared with reference to survival time and therapeutic response. Results: The experimental group (single lobaplatin as chemotherapy drug) was superior to control group (single pirarubicin hydrochloride as chemotherapy drug) in the aspects of survival time and therapeutic response, with statistical significance. Conclusions: Single lobaplatin can be as a chemotherapy drug in TACE and has better efficiency in the aspects of mean survival time and therapeutic response, deserving to be popularized in the clinic.

• Korean Journal of Head & Neck Oncology
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• v.4 no.1
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• pp.41-51
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• 1988

To evaluate the result of radiation therapy for twenty years experience, a total of 115 cases of pathologically proven glottic carcinoma had been analyzed according tot survival respectively. All the patients had been treated with radiation therapy in curative intent using Co-60 teletherapy machine. The results are as follows: 1) Accoridng to sex, 5YSR & 10YSR were 58.7% and 50.4% in male patients, and 80.0% & 72.0% in female. 2) According to T staging, 5YSR & 10YSR were 83.4% & 83.4% in T1, 69.2% & 60.0% in T2, 34.3% & 21.8% in T3, 32.0% & 0% in T4. 3) According to N staging, 5YSR & 10YSR were 63.8% & 59.2% in node-negative groups, and 30.7% & 15.4% in node-positive groups. 4) According to the histologic grade, 5YSR & 10YSR were 66.8% & 57.6% in G1, 61.3% 54.3% in G2, and 35.0% 35.0% in G3. 5) According to AJC staging, 5YSR & 10YSR were 83.4%% 83.4% in stage I, 72.0% & 62.7% in stage II, 36.8% & 28.3% in stage III, and 14.3% & 7.1% in stage IV. 6) In summary, 5YSR & 10YSR wre 60.4% & 52.8% in glottic carcinoma.

• The Korean Journal of Pain
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• v.27 no.3
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• pp.219-228
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• 2014

Background: A lipo-prostaglandin E1 agonist is effective for the treatment of neurological symptoms of spinal stenosis when administered by an oral or intravenous route. we would like to reveal the therapeutic effect of an epidural injection of lipo-prostaglandin E1 on hyperalgesia in foraminal stenosis. Methods: A total of 40 male Sprague-Dawley rats were included. A small stainless steel rod was inserted into the L5/L6 intervertebral foramen to produce intervertebral foraminal stenosis and chronic compression of the dorsal root ganglia (DRG). The rats were divided into three groups: epidural PGE1 (EP) (n = 15), saline (n = 15), and control (n = 10). In the EP group, $0.15{\mu}g{\cdot}kg-1$ of a lipo-PGE1 agonist was injected daily via an epidural catheter for 10 days from postoperative day 3. In the saline group, saline was injected. Behavioral tests for mechanical hyperalgesia were performed for 3 weeks. Then, the target DRG was analyzed for the degree of chromatolysis, chronic inflammation, and fibrosis in light microscopic images. Results: From the fifth day after lipo-PGE1 agonist injection, the EP group showed significant recovery from mechanical hyperalgesia, which was maintained for 3 weeks (P < 0.05). Microscopic analysis showed much less chromatolysis in the EP group than in the saline or control groups. Conclusions: An epidurally administered lipo-PGE1 agonist relieved neuropathic pain, such as mechanical hyperalgesia, in a rat foraminal stenosis model, with decreasing chromatolysis in target DRG. We suggest that epidurally administered lipo-PGE1 may be a useful therapeutic candidate for patients with spinal stenosis.

• Physical Therapy Korea
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• v.4 no.1
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• pp.11-19
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• 1997

The use of therapeutic ultrasound(US) in humans with malignant neoplasms has been contraindicated in physical therapy practice. Some studies have shown that results after application of US differ according to tumor type and penetration depth. The purposes of this study were to determine the effects of US on melanoma in mice and to determine treatment dosage. Twenty-four female C57BL/6 mice, age 8 weeks. The right flank of all mice was shaved, and a 0.1 ml suspension of cells was injected subcutaneously into the animals' right flank. In this study, 24 subjects were randomly divided into three groups: experimental group 1(n=8), experimental group 2(n=8), control group(n=8). In the experimental group 1, animals received continuous 3 MHZ US treatment, administered at $2.0W/cm^2$ for five minutes. In experimental group 2, animals received continuous 3 MHz US treatment, administered at $1.0W/cm^2$ for 5 minutes. The control group received the same handling as other experimental groups, including rodent chow, water, US gel application but US head pressure without the power turned on. After 10 days treatment, all mice were killed with a potassium solution. Tumors were excised and weighed on an electrical balance and fixed in a 10% neutral buffered formalin solution. Tumor weights were smaller in experimental group 2(0.3838 g) than in the control group(0.6275 g). Tumor weights of the experimental group 1(0.015 g) were smaller than those of experimental group 2. Continuous therapeutic US decreased the weight of subcutaneous melanoma tumors in mice. The treatment dosage($2.0W/cm^2$) we suggest was more effective than earlier studies on decreasing tumor size with ultrasound.