• 대한수의학회지
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• 제45권3호
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• pp.351-357
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• 2005

Here I describe a multiplex allele specific PCR-based approach for the rapid detection between Hanwoo and Holstein meat associated with Melanocortin 1 receptor (MC1R) gene. Specific and universal oligonucleotide primers were used in combination to detect the presence of a single nucleotide polymorphism within the bovine MC1R DNA sequence. The presence of the bovine MC1R gene is indicated by the production of a single control PCR product, whilst positive samples generate an alternative smaller specific product over the same region. The mutations in MC1R104 codon revealed depending on the presence or absence of an indicative fragment amplified from the wild-type allele of this codon. As little as 0.39 ng and 1.56 ng of genomic DNA of Hanwoo and Holstein could be detected by MAS-PCR assay, respectively. This technique, which is widely used in human genetic screening, provides a reliable and sensitive result that has not been documented for the identification of bovine coat color. The MAS-PCR assay approach was proven to be useful in complementing routine beef DNA analysis for differentiation of these MC1R variants and it would facilitate the screening of deceiving sales of Holstein meat in the butcher shop.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 하계학술대회 논문집 B
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• pp.1151-1154
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• 2000

Phase angle control ac drives system gains a high popularity due to their simple implementation despite the disadvantage of their poor input power factor especially for large values of phase delay angle. Furthermore it generates subharmononic current at specific phase angle. As input current of do drive systems are sinusoidal, the power factor and subharmonic current characteristics are improve. This paper presents the application of a PWM control technique of do chopper system to reduce the subharmonic current and its characteristics using single-phase dc chopper drive system of universal motor.

• Clinical Psychopharmacology and Neuroscience
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• 제16권4호
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• pp.489-493
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• 2018

Objective: This study was to identify whether working memory (WM) can be clearly subdivided according to auditory and visual modality. To do this, we administered the most recent and universal clinical WM measures in a mixed psychiatric sample. Methods: A total of 115 patients were diagnosed on the basis of DSM-IV diagnostic criteria and with MINI-Plus 5.0, a structured diagnostic interview. WM subtests of Korean version of Wechsler Adult Intelligence Scale-IV and Korean version of Wechsler Memory Scale-IV were administered to assess WM. Confirmatory factor analysis (CFA) was used to observe whether WM measures fit better to a one-factor or two-factor model. Results: CFA results demonstrated that a two factor model fits the data better than one-factor model as expected. Conclusion: Our study supports a modality model of WM, or the existence of modality-specific WM systems, and thus poses a clinical significance of assessing both auditory and visual WM tests.

• Journal of Microbiology and Biotechnology
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• 제16권5호
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• pp.740-747
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• 2006

Amplification by universal consensus sequences in pathogenic bacterial DNA would allow rapid identification of pathogenic bacteria, and amplification of genus-specific and species-specific sequences of pathogenic bacterial DNA might be used for genotyping at the genus and species levels. For design of probes for molecular diagnostics, several tools are available as stand-alone programs or as Web application. However, since most programs can design only a few probe sets at one time, they are not suitable for large-scale and automatic probes design. Therefore, for high-throughput design of specific probes in diagnostic array development, an automated design tool is necessary. Thus, we developed a Web-based automatic system for design of genus-specific and species-specific probes for pathogen detection. The system is available at http://www.miprobe.com.

• 한국산학기술학회논문지
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• 제17권8호
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• pp.423-432
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• 2016

서울 지하철 노선망의 확충은 환승역이 증가하고 복합화 지상건물과의 연계로 지하공간은 복잡해졌고 이용자의 길찾기 동선(Wayfinding)을 유도하는 사인시스템은 여러 종류의 기능이 필요에 따라 중첩, 적용해옴에 따라 피로도가 증가하였다. 본 연구의 목적은 서울지하철 역사공간을 대상으로 유니버설 디자인(UD) 원칙에 따른 사인시스템을 조사 분석하여 개선방안을 제시하는데 있다. 유니버설사인시스템을 분석하여 접근성, 안전성, 공평성, 인지성, 심미성 등을 도출하였다. 현장조사결과 천장형 방향유도 표지판류, 벽체형 보조안내판류, 종합안내도류가 정보위계가 높은 사인으로 분석되었다. 설문응답자의 유니버설 디자인개념에 대한 인식정도는 매우 낮았으나 공공시설 설계시 그 원리적용은 매우 중요시한다고 나타났다. IPA 분석결과, 접근성 측면에서 사인의 간격 및 일관된 배치, 위치 등에 대한 사항이 중점개선사항으로 도출되었고 사인의 통일성 확보 및 주변 환경과의 조화 등 심미성이 추가로 개선요망사항으로 제시되었다. 종합안내도의 경우 외부시설물 위치에 대한 적절한 안내 및 사인의 영역화 사항이 가장 시급한 개선사항으로 분석되었다. 결과적으로 모든 역에 해당하는 공통사항과 각 역에 따른 특성을 함께 반영한 유니버설 디자인개념을 적용한 지침수립이 필요함을 암시한다.

• Journal of Microbiology and Biotechnology
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• 제8권2호
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• pp.182-184
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• 1998

PCR was used for quantitative counting of Bifidobacterium spp. in a sample mixed with Lactobacillus acidophilus using two primer sets; one set for universal priming and the other set for Bifidobacterium specific priming. DNA products from two independent PCRs with DNA extracted from the mixed sample were found to be easily distinguishable from each other by agarose gel electrophoresis. The concentrations of PCR products correlated with the total number of bacteria and with the number of Bifidobacterium spp. present in the sample.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3469-3476
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• 2016

Background: The cancer research literature suggests that women, especially premenopausal women, have lower cancer mortality rates than men. However, it is unclear if that is true for populations at all age levels in all countries and what factors affect such sex differences. This paper attempts to fill that gap. Materials and Methods: Sex- and country-specific cancer mortality data were statistically analyzed with particular attention to geographic, social, and economic factors that may affect the sex differences. Results: The sex differences were age and country specific, rather than universal. Premenopausal women actually tend to have a disadvantage compared to men or postmenopausal women. Male cancer mortality appears to be the affecting factor in explaining variations in sex differences. Latitude of residence and literacy rate are the affecting factors in cancer mortality and sex differences. African and Latin American countries tend to have a female disadvantage, while East Asian and Eastern European countries are more likely to have a female advantage. Conclusions: The findings challenge the cancer mortality literature and indicate that the sex differences and their possible causes are more complicated than the current literature suggests. They also highlight the urgency of adapting age- and country- specific health systems and policies to better meet the needs of younger women.

• Clinical and Experimental Pediatrics
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• 제65권3호
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• pp.108-114
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• 2022

The Japanese encephalitis (JE) virus is the leading cause of vaccine-preventable encephalitis in Asia. Since the introduction of a universal JE vaccination program and urbanization of Korea, the incidence of JE has dramatically decreased in Korea. However, recent JE cases have occurred, predominantly among unvaccinated adults and with a shift in age distribution. Here we aimed to review the changes in age-specific JE seroprevalence over time and discuss the implications of JE vaccination programs in Korea. Following the last epidemic in 1982-1983, mandatory vaccination for all children aged 3-15 years was conducted annually until 1994. However, JE has reemerged, predominantly affecting unvaccinated adults aged 40 years or older and demonstrating a shift in age distribution toward older populations. The age-specific seroprevalence of the JE virus in Korea has changed noticeably over time. Seropositivity in children and adolescents increased from 10%-59% in the 1970s to 90%-92% in the 1980s after the implementation of the JE vaccination program and increased further to 98% in 2012. No age-specific difference in the seroprevalence of JE was found, and appropriate levels of immunity to JE were maintained for all age groups. Continuous surveillance of the seroprevalence of JE is essential to establish a proper immunization policy in Korea.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1170-1177
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• 2005

Terminal-restriction fragment length polymorphism (T-RFLP) analysis was used to monitor inoculated oil-degrading microorganisms during bioremedial treatability tests. A pair of universal primers, fluorescently labeled 521F and 1392R, was employed to amplify small subunit rDNA in order to simultaneously detect two bacterial strains, Corynebacterium sp. IC10 and Sphingomonas sp. KH3-2, and a yeast strain, Yarrowia lipolytica 180. Digestion of the 5'-end fluorescence/labeled PCR products with HhaI produced specific terminal-restriction fragments (T-RFs) of 185 and 442 bases, corresponding to Corynebacterium sp. IC10 and Y. lipolytica 180, respectively. The enzyme NruI produced a specific T-RF of 338 bases for Sphingomonas sp. KH3-2. The detection limit for oildegrading microorganisms that were inoculated into natural environments was determined to be $0.01\%$ of the total microbial count, regardless of the background environment. When three oil-degrading microorganisms were released into oil-contaminated sand microenvironments, strains IC10 and 180 survived for 35 days after inoculation, whereas strain KH3-2 was detected at 8 days, but not at 35 days. This result implies that T-RFLP could be a useful tool for monitoring the survival and relative abundance of specific microbial strains inoculated into contaminated environments.

• 한국언어정보학회지:언어와정보
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• 제2권1호
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• pp.214-255
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• 1998

Close examination of previous constraints on bound anaphora that are designed to directly constrain the distribution of referentially dependent(RD) items shows that no universal structural relation may exist that relates RD items to their antecedents. As an alternative to these constraints, this paper proposes an axiom of semantic interpretation, called the Principle of Referential Autonomy, which dispenses with any pretheoretical notion of grammatical functions or configurational notion like c-command. Together with certain English-specific facts, this principle enables us to infer the ungrammaticality of core examples of strong and weak crossover.