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Rutin alleviated lipopolysaccharide-induced damage in goat rumen epithelial cells

  • Jinshun Zhan;Zhiyong Gu;Haibo Wang;Yuhang Liu;Yanping Wu;Junhong Huo
    • Animal Bioscience
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    • v.37 no.2
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    • pp.303-314
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    • 2024
  • Objective: Rutin, also called vitamin P, is a flavonoids from plants. Previous studies have indicated that rutin can alleviate the injury of tissues and cells by inhibiting oxidative stress and ameliorating inflammation. There is no report on the protective effects of rutin on goat rumen epithelial cells (GRECs) at present. Hence, we investigated whether rutin can alleviate lipopolysaccharide (LPS)-induced damage in GRECs. Methods: GRECs were cultured in basal medium or basal medium containing 1 ㎍/mL LPS, or 1 ㎍/mL LPS and 20 ㎍/mL rutin. Six replicates were performed for each group. After 3-h culture, the GRECs were harvested to detect the relevant parameters. Results: Rutin significantly enhanced the cell activity (p<0.05) and transepithelial electrical resistance (TEER) (p<0.01) and significantly reduced the apoptosis rate (p<0.05) of LPS-induced GRECs. Rutin significantly increased superoxide dismutase, glutathione peroxidase, and catalase activity (p<0.01) and significantly decreased lactate dehydrogenase activity and reactive oxygen species and malondialdehyde (MDA) levels in LPS-induced GRECs (p<0.01). The mRNA and protein levels of interleukin 6 (IL-6), IL-1β, and C-X-C motif chemokine ligand 8 (CXCL8) and the mRNA level of tumor necrosis factor-α (TNF-α) and chemokine C-C motif ligand 5 (CCL5) were significantly increased in LPS-induced GRECs (p<0.05 or p<0.01), while rutin supplementation significantly decreased the mRNA and protein levels of IL-6, TNF-α, and CXCL8 in LPS-induced GRECs (p<0.05 or p<0.01). The mRNA level of toll-like receptor 2 (TLR2), and the mRNA and protein levels of TLR4 and nuclear factor κB (NF-κB) was significantly improved in LPS-induced GRECs (p<0.05 or p<0.01), whereas rutin supplementation could significantly reduce the mRNA and protein levels of TLR4 (p<0.05 or p<0.01). In addition, rutin had a tendency of decreasing the protein levels of CXCL6, NF-κB, and inhibitor of nuclear factor kappa-B alpha (0.05

The Preparation of Reversed Micelle Containing Water Soluble Collagen Solution and Their Application on Lip Make up Products (콜라겐 수용액을 함유하는 역미셀의 제조 및 립 제품에 응용)

  • Kim, Young-Ho;Jung, Eun-Ji;Lee, Dong-Won;Lee, Sang-Gil;Pyo, Hyeong-Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.39 no.4
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    • pp.271-279
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    • 2013
  • Water-soluble collagen (30 wt%) was entrapped within oil phase of lipstick using reversed micelle method to improve the moisture capacity of the lipstick. Reversed micelles containing collagen were prepared using caprylic/capric triglyceride as external phase and polyoxyethylene (10) octylphenyl ether (Triton X-100) and 1-dodecanol as surfactant and co-surfactant, respectively. The formation of reversed micelle encapsulating collagen was confirmed by measuring electric conductivity and UV-vis spectrum using methylene blue (MB). The stability and moisture capacity of the lipstick containing 20 wt% collagen encapsulated reversed micelles were observed by measuring rheology property, moisture content and amino acid content. The molecular ratio (W, water-pool) of water to surfactant (Triton X-100) in the most stable reversed micelle was ${\leq}$ 10. The hardness of the lipstick had no difference with that of the lipstick without reversed micelle, and the moisture content was increased to 59% and the amino acid content was 92.7%.

Multiscale Virtual Testing Machines of Concrete and Other Composite Materials: A Review (콘크리트 및 복합재료용 멀티스케일 가상 시험기계에 관한 소고)

  • Haile, Bezawit F.;Park, S.M.;Yang, B.J.;Lee, H.K.
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.31 no.4
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    • pp.173-181
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    • 2018
  • Recently composite materials have dominated most engineering fields, owing to their better performance, increased durability and flexibility to be customized and designed for a specific required property. This has given them unprecedented superiority over conventional materials. With the help of the ever increasing computational capabilities of computers, researchers have been trying to develop accurate material models for the complex and integrated properties of these composites. This has led to advances in virtual testing of composite materials as a supplement or a possible replacement of laboratory experiments to predict the properties and responses of composite materials and structures. This paper presents a review on the complex multi-scale modelling framework of the virtual testing machines, which involve computational mechanics at various length-scales starting with nano-mechanics and ending in structure level computational mechanics, with a homogenization technique used to link the different length scales. In addition, the paper presents the features of some of the biggest integrated virtual testing machines developed for study of concrete, including a multiscale modeling scheme for the simulation of the constitutive properties of nanocomposites. Finally, the current challenges and future development potentials for virtual test machines are discussed.

An Efficient Coding Technique of Holographic Video Signal using 3D Segment Scanning (분할영역의 3차원 스캐닝을 이용한 홀로그래픽 비디오 신호의 효율적인 부호화 기술)

  • Seo, Young-Ho;Choi, Hyun-Jun;Kim, Dong-Wook
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.2C
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    • pp.132-140
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    • 2007
  • In this paper, we proposed a new technique to encode and decode the digital hologram. Since the digital hologram (or fringe pattern) is generated by interference of light, it has much different property from natural 2D (2 dimensional) images. First, we acquisite optical-sensed or computer-generated hologram by digital type, and then extract a chrominance component. The extracted digital hologram for coding is separated into segments to use multi-view properties. The segmented hologram shows the similar characteristics with picturing an object with 2D cameras in various point of view. Since fringe pattern is visually observed like as noise, we expect that the fringe pattern has poor coding efficiency. To obtain high efficiency, the segment is transformed with DCT (Discrete Cosine Transform) which resembles hologram generation process with high performance. Each transformed segment passes the 3D scanning process according to time and spatial correlation, and is organized into a video stream. Since the segment which correspond to frame of a video stream consists of the transformed coefficients with wide range of value, it is classified and re-normalized. Finally it is compressed with coding tools. The proposed algorithm illustrated that it has better properties for reconstruction of 16 times higher compression rate than the previous researches.

The Effect of Post-Bond Heat Treatment on Tensile Property of Diffusion Bonded Austenitic Alloys (확산 접합된 오스테나이트계 재료의 인장특성에 미치는 후열처리의 영향)

  • Hong, Sunghoon;Kim, Sung Hwan;Jang, Changheui;Sah, Injin
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.39 no.12
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    • pp.1221-1227
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    • 2015
  • Diffusion bonding is the key manufacturing process for the micro-channel type heat exchangers. In this study, austenitic alloys such as Alloy 800HT, Alloy 690, and Alloy 600, were diffusion bonded at various temperatures and the tensile properties were measured up to $650^{\circ}C$. Tensile ductility of diffusion bonded Alloy 800HT was significantly lower than that of base metal at all test temperatures. While, for Alloy 690 and Alloy 600, tensile ductility of diffusion bonded specimens was comparable to that of base metals up to $500^{\circ}C$, above which the ductility became lower. The poor ductility of diffusion bonded specimen could have caused by the incomplete grain boundary migration and precipitates along the bond-line. Application of post-bond heat treatment (PBHT) improved the ductility close to that of base metals up to $550^{\circ}C$. Changes in tensile properties were discussed in view of the microstructure in the diffusion-bonded area.

Change of Fermentation Characteristic of Kimchi by n-Capric Acid or n-Capric Acid Methyl Ester Addition and Storage Temperature (n-Capric Acid 또는 n-Capric Acid Methyl Ester 첨가와 저장온도에 따른 김치의 발효특성)

  • Kim, Byung-Yong;Ahn, Tae-Young;Kim, Myung-Hwan
    • Applied Biological Chemistry
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    • v.42 no.2
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    • pp.156-161
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    • 1999
  • This study was conducted to investigate the effects of n-capric acid(CA) or n-capric acid methyl ester (CE) addition during salting process, and fermentation temperature on chemical and microbial changes of Kimchi. The pHs of control, CA and CE were 3.78, 4.28 and 4.35 after 6 days of storage at $20^{\circ}C$ and were 3.85, 5.14 and 5.10 after 42 days of storage at $4^{\circ}C$, respectively. The effects of CA or CE addition at $4^{\circ}C$ were higher than those at $20^{\circ}C$. The maximum edible acidity, 0.75%, was reached within 3 days at $20^{\circ}C$, 15 days at $12^{\circ}C$, and the acidity of 42 days at $4^{\circ}C$ was 0.62% which was still lower value than the maximum edible acidity. Total bacteria, Leuconostoc, Lactobacillus and yeast counts of the control were higher value than those of the treatments throught the whole fermentation period. The addition of CE at $4^{\circ}C$ had much affected the reduction of yeast count. As microbial counts and reduction rates of control and treated Kimchi increased with increasing the storage temperature.

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Design and Fabrication of the 0.1${\mu}{\textrm}{m}$ Г-Shaped Gate PHEMT`s for Millimeter-Waves

  • Lee, Seong-Dae;Kim, Sung-Chan;Lee, Bok-Hyoung;Sul, Woo-Suk;Lim, Byeong-Ok;Dan-An;Yoon, yong-soon;kim, Sam-Dong;Shin, Dong-Hoon;Rhee, Jin-koo
    • Journal of electromagnetic engineering and science
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    • v.1 no.1
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    • pp.73-77
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    • 2001
  • We studied the fabrication of GaAs-based pseudomorphic high electron mobility transistors(PHEMT`s) for the purpose of millimeter- wave applications. To fabricate the high performance GaAs-based PHEMT`s, we performed the simulation to analyze the designed epitaxial-structures. Each unit processes, such as 0.1 m$\mu$$\Gamma$-gate lithography, silicon nitride passivation and air-bridge process were developed to achieve high performance device characteristics. The DC characteristics of the PHEMT`s were measured at a 70 $\mu$m unit gate width of 2 gate fingers, and showed a good pinch-off property ($V_p$= -1.75 V) and a drain-source saturation current density ($I_{dss}$) of 450 mA/mm. Maximum extrinsic transconductance $(g_m)$ was 363.6 mS/mm at $V_{gs}$ = -0.7 V, $V_{ds}$ = 1.5 V, and $I_{ds}$ =0.5 $I_{dss}$. The RF measurements were performed in the frequency range of 1.0~50 GHz. For this measurement, the drain and gate voltage were 1.5 V and -0.7 V, respectively. At 50 GHz, 9.2 dB of maximum stable gain (MSG) and 3.2 dB of $S_{21}$ gain were obtained, respectively. A current gain cut-off frequency $(f_T)$ of 106 GHz and a maximum frequency of oscillation $(f_{max})$ of 160 GHz were achieved from the fabricated PHEMT\\`s of 0.1 m$\mu$ gate length.h.

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Physicochemical and Sensory Characteristics of Dongchimi Prepared with Turnip of Chinese, European and Korean Origin (한국.중국.유럽산 순무로 담근 동치미의 이화학적.관능적 특성 비교)

  • 오상희;김미리
    • Journal of the East Asian Society of Dietary Life
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    • v.13 no.2
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    • pp.111-121
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    • 2003
  • Physicochemical and sensory characteristics of turnip Donchimi prepared with Chinese(DC), Korean(DK) and European origin(DE) were analyzed during fermentation at $0^{\circ}C$. Salt concentrations of Dongchimi liquid were around 1.3% during fermentation. The pH, the total acidity, Lactobacilli number and turbidity were observed during fermentation of Dongchmi. DC was fermented the most rapidly among three based on the acidity, turbidity and lactic acid bacteria number. DC maintained higher hardness in textural properties during fermentation compared to DE, although it was evaluated lower scores of appearance in sensory test, and less Hunter color a value and anthocyanin content, compared to DK or DE. DK showed the highest in anthocyanin content, Hunter color a value, and hardness. Sensory evaluation showed that the scores of color, hardness, and overall preference of Donchimi were high in the order of DK>DC>DE. Score of overall preference of turnip Dongchimi was the highest with 6.7(10 cm line scale) in DK at the 40th day of fermentation, the optimum ripening time, but the lowest with 3.5 in DE.

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Correlation between the Factors of Soil Physical Property in Upland Soil (밭작물 토양물리성 지표관련 인자의 상관분석)

  • Kim, Chan-Yong;Seo, Young-Jin;Kwon, Tae-Young;Park, Jun-Hong;Heo, Min-Soon;Ha, Sang-Keun
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.793-797
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    • 2010
  • The investigations were conducted to improve the physical properties by analyzing physico-chemical properties on the different soil families of textures at 20 upland fields located in the parts of Gyengsangbuk-do area. Soil physico-chemical properties were analyzed for bulk density, hardness, porosity, moisture, pH, EC and organic mater by soil depth on the different soil families of textures. Bulk density distributions were higher than 1.2 Mg $m^{-3}$ in the optimum range. Hardness distributions were lower than 20 mm in the optimum range. Therefore, the physical properties of upland soil was deteriorated. Correlation coefficient of bulk density with hardness and organic mater were higher significantly, that was positive and negative, respectively. The soil hardness had the greatest distribution degree to the crop yield and bulk density and organic matter followed. Conclusively, To improve the physical properties of upland soil was more effective to fertilizing organic matter than other ways.

Effects of Activator on Rubber Characteristics for Gasket to Lithium Ion Battery (리튬 이온 전지용 개스킷 고무 물성에 미치는 가교조제의 영향)

  • Kang, Dong-gug;Kim, Hye-young;Kang, Young-im;Hur, Byung-ki;Seo, Kwan-ho
    • Applied Chemistry for Engineering
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    • v.22 no.4
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    • pp.395-399
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    • 2011
  • Material of the gasket for lithium ion battery requires the chemical resistance, the electrical insulting property, the compression set, the anti-contamination level and the low temperature resistance. We compounded ethylene propylene diene monomer (EPDM), which showed widely different solubility parameter index, with adjusting the amount of metal oxide as an activator. We did long-term test and compression set against an electrolyte with consideration for operating conditions in lithium-ion battery. In these tests, we checked the physical, chemical characteristics and the effect to lithium ion battery with different kinds of activators. In case of rubber with ZnO as an activator, through 1000 h depositing test in propylene carbonate which is one of representative solvents, we could get the satisfying characteristics and result. However, $Zn^{2+}$ had eluted in the ion elution test. So, ZnO should be limited in EPDM compound for the gasket material in lithium-ion battery.