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Effect of Casing Layer on Growth Promotion of the Edible Mushroom Pleurotus ostreatus

  • Cho, Young-Sub;Weon, Hang-Yeon;Joh, Jung-Ho;Lim, Jong-Hyun;Kim, Kyung-Yun;Son, Eun-Suk;Lee, Chang-Soo;Cho, Bong-Gum
    • Mycobiology
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    • v.36 no.1
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    • pp.40-44
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    • 2008
  • Various bacteria were isolated from the casing layer soil of the culture bed of P. ostreatus and their role in fruiting body induction of the edible mushroom, P. ostreatus, was investigated. Analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. Bordetella was predominant in the bulk soil whereas Flavobacterium was predominant after sterilization of the casing layer soil. Fluorescent Pseudomonas was predominant in the non-sterilized casing layer soil. Total number of the bacterial genera in the casing layer soil was higher than that in the bulk soil. In particular, an increase in the fluorescent Pseudomonas population was observed in the non-sterilized casing layer accompanied by induction of fruiting body and enhanced mushroom production yield. The results suggested that specific bacterial populations in the casing layer play an important role in the formation of primodia and the development of basidiome in P. ostreatus.

Numerical Study on the Wind Flow Over Hilly Terrain (언덕지형을 지나는 유동의 수치해석적 연구)

  • 김현구;이정묵;경남호
    • Journal of Korean Society for Atmospheric Environment
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    • v.13 no.1
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    • pp.65-77
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    • 1997
  • A theoretical and numerical investigation on the boundary-layer flow over a two- or three-dimensional hill is presented. The numerical model is based on the finite volume method with boundary-fitted coordinates. The k-$\varepsilon$ turbulence model with modified wall function and the low-Reynolds-number model are employed. The hypothesis of Reynolds number independency for the atmospheric boundary-layer flow over aerodynamically rough terrain is confirmed by the numerical simulation. Comparisons of the mean velocity profiles and surface pressure distributions between the numerical predictions and the wind-tunnel experiments on the flow over a hill show good agreement. The linear theory provides generally good prediction of speed-up characteristics for the gentle-sloped hills. The flow separation occurs in the hill slope of 0.5 and the measured reattachment points are compared with the numerical prediction. It is found that the k- $\varepsilon$ turbulence model is reasonably accurate in predicting the attached flow, while the low- Reynolds-number model is more suitable to simulate the separated flows.ows.

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Damage detection in structures using modal curvatures gapped smoothing method and deep learning

  • Nguyen, Duong Huong;Bui-Tien, T.;Roeck, Guido De;Wahab, Magd Abdel
    • Structural Engineering and Mechanics
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    • v.77 no.1
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    • pp.47-56
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    • 2021
  • This paper deals with damage detection using a Gapped Smoothing Method (GSM) combined with deep learning. Convolutional Neural Network (CNN) is a model of deep learning. CNN has an input layer, an output layer, and a number of hidden layers that consist of convolutional layers. The input layer is a tensor with shape (number of images) × (image width) × (image height) × (image depth). An activation function is applied each time to this tensor passing through a hidden layer and the last layer is the fully connected layer. After the fully connected layer, the output layer, which is the final layer, is predicted by CNN. In this paper, a complete machine learning system is introduced. The training data was taken from a Finite Element (FE) model. The input images are the contour plots of curvature gapped smooth damage index. A free-free beam is used as a case study. In the first step, the FE model of the beam was used to generate data. The collected data were then divided into two parts, i.e. 70% for training and 30% for validation. In the second step, the proposed CNN was trained using training data and then validated using available data. Furthermore, a vibration experiment on steel damaged beam in free-free support condition was carried out in the laboratory to test the method. A total number of 15 accelerometers were set up to measure the mode shapes and calculate the curvature gapped smooth of the damaged beam. Two scenarios were introduced with different severities of the damage. The results showed that the trained CNN was successful in detecting the location as well as the severity of the damage in the experimental damaged beam.

Using Neural Networks to Predict the Sense of Touch of Polyurethane Coated Fabrics (신경망이론은 이용한 폴리우레탄 코팅포 촉감의 예측)

  • 이정순;신혜원
    • Journal of the Korean Society of Clothing and Textiles
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    • v.26 no.1
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    • pp.152-159
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    • 2002
  • Neural networks are used to predict the sense of touch of polyurethane coated fabrics. In this study, we used the multi layer perceptron (MLP) neural networks in Neural Connection. The learning algorithm for neural networks is back-propagation algorithm. We used 29 polyurethane coated fabrics to train the neural networks and 4 samples to test the neural networks. Input variables are 17 mechanical properties measured with KES-FB system, and output variable is the sense of touch of polyurethane coated fabrics. The influence of MLF function, the number of hidden layers, and the number of hidden nodes on the prediction accuracy is investigated. The results were as follows: MLP function, the number of hidden layer and the number of hidden nodes have some influence on the prediction accuracy. In this work, tangent function, the architecture of the double hidden layers and the 24-12-hidden nodes has the best prediction accuracy with the lowest RMS error. Using the neural networks to predict the sense of touch of polyurethane coated fabrics has hotter prediction accuracy than regression approach used in our previous study.

Studies on the Microbial Population and the Amylase Activity of the Forest Soil (삼림토양의 미생물군집과 아밀라아제 활성에 관한 연구)

  • Lee, Hee-Sun;Shim, Jae-Kuk
    • The Korean Journal of Ecology
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    • v.17 no.2
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    • pp.171-183
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    • 1994
  • Soil condition, total number of bacteria, soil amylase activity and microbial biomass $(CO_2-C)$ were measured at soil of different forest types. And the difference of the allelopathic effect was determined between fresh leaf extract of Quercus acutissima and Pinus rigida to the bacteria isolated from soil of different forest types. 1. Total number of bacteria in Carpinus laxiflora forest soil was 4~7 times larger than that in pinus desiflora forest soil. 2. Soil amylase activity was positively correlated with total number of soil bacteria and soil organic matter content. The amylase activity at F layer was 4~5 times larger than that at H layer, and that at H layer was 2~4 times larger than that at A layer. 3. Seasonal changes of microbial biomass showed a peak in summer, and vertical distribution of microbial biomass decreased with increasing soil depth. The microbial biomass in Pinus densiflora forest soil was larger than that in Quercus serrata forest soil. 4. Fresh leaf extract of Pinus rigida and Quercus acutissima showed an acceleration or inhibition effect on the growth of soil bacteria, and that of !. acutissima inhibited larger number of soil bacterial strains than that of P. rigida. 4.2% and 25% of soil bacterial strains isolated from soil of P. rigida and Q. acutissima forests were inhibited by fresh leaf extract of P. rigida and Q. acutissima, respectively.

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Characteristics of the plume formed by the buoyant discharges from the river

  • Kim, Ki-Cheol;Kim, Sung-Bo
    • Journal of Advanced Marine Engineering and Technology
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    • v.38 no.8
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    • pp.981-994
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    • 2014
  • Density currents formed by buoyancy discharges from rivers are numerically studied using non-dimensional two layer model including Coriolis acceleration, bottom stress, interfacial friction. Some typical numbers such as Froude number, densimetric Froude number and Kelvin number are obtained and some characteristic scales are defined as a result of non-dimensionalization of the governing equations. Besides the Coriolis effect, the configurations of bottom topography, bottom friction coefficient and interfacial friction are found to significantly affect the propagation of the warm water plume. Frontal position can fastly propagate in the case of large density difference between the two layers and small interfacial friction. Left side boundary current is easily formed under the small interfacial friction. With large Kelvin number, both right and left side boundary currents are formed. Wave-like disturbances and eddies are easily formed under the high Froude number.

Reduction of Normal Shock-Wave Oscillations by Turbulent Boundary Layer Flow Suction (경계층 유동의 흡입에 의한 수직충격파 진동저감)

  • Kim, Heuy Dong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.22 no.9
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    • pp.1229-1237
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    • 1998
  • Experiments of shock-wave/turbulent boundary layer interaction were conducted by using a supersonic wind tunnel. Nominal Mach number was varied in the range of 1.6 to 3.0 by means of different nozzles. The objective of the present study is to investigate the effects of boundary layer suction on normal shock-wave oscillations caused by shock wave/boundary layer interaction in a straight duct. Two-dimensional slits were installed on the top and bottom walls of the duct to bleed turbulent boundary layer flows. The bleed flows were measured by an orifice. The ratio of the bleed mass flow to main mass flow was controlled below the range of 11 per cent. Time-mean and fluctuating wall pressures were measured, and Schlieren optical observations were made to investigate time-mean flow field. Time variations in the shock wave displacement were obtained by a high-speed camera system. The results show that boundary layer suction by slits considerably reduce shock-wave oscillations. For the design Mach number of 2.3, the maximum amplitude of the oscillating shock-wave reduces by about 75% compared with the case of no slit for boundary layer suction.

한국과 일본의 산란계 산업 비교

  • Jang, Gyeong-Man;Yun, Byeong-Seon
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2005.11a
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    • pp.82-83
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    • 2005
  • Recently, layer industry shows the decrease in the number of farm and increase in rearing scale in both Korea and Japan. Seventy percent of layer farms in Japan feed more than 50,000 birds per each farm but in Korea, only 19.3% of layer farms are feeding more than 30,000 birds which covers 59% of total chicken number. This tendeney will be more accellerated from now on. Brand egg Production in Japan is developed with the involvement of farmers, sailers feed companies and also the marketing structure of brand egg is well organised in between farmers and consumers. However in Korea, the marketing structure of brand egg is not well settled for this reason the egg price is often decided lower much price than regrnlar price by the intermediate dealers. Under this kind of tough situation, Korean layer farmers are stressed to develop new brand products such as functional egg, settle the sales and distribution network with the good cooperation of farmers, and distributers.

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Keratinocyte Proliferation in Aged Rat Skin by High Voltage Pulsed Current Stimulation

  • Lee Jae-Hyoung;Lee Jong-Sook;Kil Eyn-Young
    • Biomedical Science Letters
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    • v.10 no.4
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    • pp.361-366
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    • 2004
  • The purpose of this study was to determine the effect of high voltage pulsed current (HVPC) stimulation on proliferative activities of basal keratinocytes by measured nucleolar organizer region (NOR) expression and thickness of spinous layer in aged rat skin. Fifty-one weeks old twelve male Sprague-Dawley rats (300∼350g) were divided into control and HVPC stimulation groups. Each animal's hair on the back were removed. The HVPC stimulation group received an negative monophasic twin peak pulsed current stimulation with 50 V, while the control group was given the same treatment without electricity. The rats were sacrificed after 3 weeks. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with hematoxyline-eosin and silver nitrate. The thickness of basal to granular layer of the epidennis were measured using a light microscope and computerized image analysis system. The number of argyrophilic nucleolar organizer region (AgNOR) were counted using a light microscope and computerized image analysis system and calculated as the mean number of AgNOR per nucleus in the basal keratinocyte. By using a Student's t-test, an increase in the thickness of basal-spinous layer (P<0.001) of epidermis can be observed in HVPC stimulation rats as compared with the control rats, whereas the thickness of the granular layer is not affected. A Student's t-test showed a significantly higher mean NOR number per nucleus of the basal keratinocyte in the HVPC stimulation rats than control rats (P<0.001). There was significantly positive correlation between the NOR number and the thickness of basal-spinous layer (r=0.80, P<0.05). These results suggest that the HVPC stimulation may increase the thickness of spinous layer in the epidennis due to increased proliferative activities of basal keratinocytes in epidennis in aged rat skin.

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Influence of Upstream State on the Interacting Turbulent Boundary Layer (相互作용하는 亂流 境界層에 대한 上流狀態의 影響)

  • 이덕봉
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.10 no.3
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    • pp.277-284
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    • 1986
  • A numerical procedure (integral method) for calculating the interacting turbulent boundary layer is set up. With this method, some free interactions with various upstream conditions are simulated in order to investigate the influence of upstream state on the interacting turbulent boundary layer. The results obtained by this numerical simulation can be summarized as follows; Free interaction of upstream unstabilized (or separated) turbulent boundary layer is subcritical regardless of its external Mach number, while free interaction of upstream stabilized turbulent boundary layer has two different characteristics (subcritical, supercritical) according to the external Mach number.