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Cyanobacterial Diversity Shifts Induced by Butachlor in Selected Indian Rice Fields in Eastern Uttar Pradesh and Western Bihar Analyzed with PCR and DGGE

  • Kumari, Nidhi;Narayan, Om Prakash;Rai, Lal Chand
    • Journal of Microbiology and Biotechnology
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    • v.22 no.1
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    • pp.1-12
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    • 2012
  • The present study examines the effects of 30 mg/kg butachlor on the cyanobacterial diversity of rice fields in Eastern Uttar Pradesh and Western Bihar in India. A total of 40 samples were grouped into three classes [(i) acidic, (ii) neutral, and (iii) alkaline soils], based on physicochemical and principle component analyses. Acidic soils mainly harbored Westillopsis, Trichormus, Anabaenopsis, and unicellular cyanobacteria; whereas Nostoc, Anabaena, Calothrix, Tolypothrix, and Aulosira were found in neutral and alkaline soils. Molecular characterization using 16S rRNA PCR and DGGE revealed the presence of 13 different phylotypes of cyanobacteria in these samples. Butachlor treatment of the soil samples led to the disappearance of 5 and the emergence of 2 additional phylotypes. A total of 40 DGGE bands showed significant reproducible changes upon treatment with butachlor. Phylogenetic analyses divided the phylotypes into five major clusters exhibiting interesting links with soil pH. Aulosira, Anabaena, Trichormus, and Anabaenopsis were sensitive to butachlor treatment, whereas uncultured cyanobacteria, a chroococcalean member, Westillopsis, Nostoc, Calothrix, Tolypothrix, Rivularia, Gloeotrichia, Fischerella, Leptolyngbya, and Cylindrospermum, appeared to be tolerant against butachlor at their native soil pH. Butachlor-induced inhibition of nitrogen fixation was found to be 65% (maximum) and 33% (minimum) in the soil samples of pH 9.23 and 5.20, respectively. In conclusion, low butachlor doses may prove beneficial in paddy fields having a neutral to alkaline soil pH.

PCA-Based Feature Reduction for Depth Estimation (깊이 추정을 위한 PCA기반의 특징 축소)

  • Shin, Sung-Sik;Gwun, Ou-Bong
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.47 no.3
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    • pp.29-35
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    • 2010
  • This paper discusses a method that can enhance the exactness of depth estimation of an image by PCA(Principle Component Analysis) based on feature reduction through learning algorithm. In estimation of the depth of an image, hyphen such as energy of pixels and gradient of them are found, those selves and their relationship are used for depth estimation. In such a case, many features are obtained by various filter operations. If all of the obtained features are equally used without considering their contribution for depth estimation, The efficiency of depth estimation goes down. This paper proposes a method that can enhance the exactness of depth estimation of an image and its processing speed is considered as the contribution factor through PCA. The experiment shows that the proposed method(30% of an feature vector) is more exact(average 0.4%, maximum 2.5%) than using all of an image data in depth estimation.

Research Trend of Bio-oil Production from Biomass by using Fast Pyrolysis (바이오매스로부터 급속 열분해를 통한 바이오오일의 생산기술 연구동향)

  • Kim, Jae-Kon;Park, Jo Yong;Yim, Eui Soon;Ha, Jong Han
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.3
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    • pp.453-465
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    • 2014
  • The paper provides a review on bio-oil production technology from biomass by using fast pyrolysis to use heating fuel, power fuel and transport fuel. One of the most promising methods for a small scale conversion of biomass into liquid fuels is fast pyrolysis. In fast pyrolysis, bio-oil is produced by rapidly heating biomass to intermediate temperature ($450{\sim}600^{\circ}C$) in the absence of any external oxygen followed by rapid quenching of the resulting vapor. Bio-oil can be produced in weight yield maximum 75 wt% of the original dry biomass and bio-oils typically contain 60-75% of the initial energy of the biomass. In this study, it is described focusing on the characterization of feedstock, production principle of bio-oil, bio-oil's property and it's application sector.

Fuzzy Logic PID controller based on FPGA

  • Tipsuwanporn, V.;Runghimmawan, T.;Krongratana, V.;Suesut, T.;Jitnaknan, P.
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.1066-1070
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    • 2003
  • Recently technologies have created new principle and theory but the PID control system remains its popularity as the PID controller contains simple structure, including maintenance and parameter adjustment being so simple. Thus, this paper proposes auto tune PID by fuzzy logic controller based on FPGA which to achieve real time and small size circuit board. The digital PID controller design to consist of analog to digital converter which use chip TDA8763AM/3 (10 bit high-speed low power ADC), digital to analog converter which use two chip DAC08 (8 bit digital to analog converters) and fuzzy logic tune digital PID processor embedded on chip FPGA XC2S50-5tq-144. The digital PID processor was designed by fundamental PID equation which architectures including multiplier, adder, subtracter and some other logic gate. The fuzzy logic tune digital PID was designed by look up table (LUT) method which data storage into ROM refer from trial and error process. The digital PID processor verified behavior by the application program ModelSimXE. The result of simulation when input is units step and vary controller gain ($K_p$, $K_i$ and $K_d$) are similarity with theory of PID and maximum execution time is 150 ns/action at frequency are 30 MHz. The fuzzy logic tune digital PID controller based on FPGA was verified by control model of level control system which can control level into model are correctly and rapidly. Finally, this design use small size circuit board and very faster than computer and microcontroller.

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Development of an Electric Pulp Tester with Constant Current Source (정 전류원을 이용한 치수 검사기의 개발)

  • 김재성;남기창;김수찬;이승종;김덕원
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.41 no.2
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    • pp.61-68
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    • 2004
  • Electric stimulation of teeth has been used for assessing pulp vitality. The principle is based upon the assumption that a subject feels the pain produced by electrical current stimulation of intradental nerve. Because of very high and wide range of impedance of the enamel, it is very difficult to determine stimulation levels regardless of teeth status. Most pulp testers adopt voltage stimulation method and thus, their stimulating threshold levels significantly depend on each individual. Therefore, a constant current stimulator is necessary to minimize the effect of wide variation due to different enamel thickness. And it is also necessary to test teeth vitality with a wide current range regardless of tooth impedance. In this study, we constructed a burst-wave type pulp tester to reduce the pain using a current stabilizing circuit with the maximum current of 150 uA.

Development of Prototype Biosensor for The Detection of Organophosporus Compounds (유기인화합물 측정용 광바이오센서 개발)

  • 최정우;김종민;이원홍;김영기
    • KSBB Journal
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    • v.17 no.2
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    • pp.158-161
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    • 2002
  • In this study, a prototype fiber-optic biosensor was fabricated using the inhibition of enzyme reaction by organophosphorus compounds to detect organophosphorus compounds, which is nervous toxic material an? is used as chemical weapon and pesticide. Enzyme, substrate, and inhibitor for enzyme reaction were acetylcholinesterase (key enzyme in nervous cell), acetylthiocholine iodide, and paraoxon (a kind of organophosphorus compounds), respectively. The detection principle of sensor is the detection of enzyme reaction inhibited by organophosphorus compounds by the quantitative measurement of acetic acid, which was achieved by absorbance measurement using litmus solution that maximum absorbance band is changed by pH. To fabricate prototype fiber-optic biosensor, high bright LED and photodiode was used as light source and light intensity detector, respectively. From the experimental results using a prototype biosensor, the linear change of sensor signal was obtained in a range of 0-2 ppm inhibitor concentrations. From these results, it was verified that the quantitative measurement of organophosphorus compounds could be achieved fast (within 2 minutes) and accurately by a prototype fiber-optic biosensor.

Response of Mulberry Brown Leaf Spot Fungus Myrothecium roridum to Different Plant Extracts

  • Chattopadhyay, S.;Institute, Traning;Majil, M.D.;Pratheesshkumar;Das, K.K.;Saratchandra, B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.2
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    • pp.183-188
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    • 2002
  • Anti-fungal potential of 5 plant extracts viz., Eucalyptus citriodora, Allium sativum, Cassia sophera, Chromolaena odorata and Datura metel on the growth of mulberry brown leaf spot pathogen Myrothecium roridum were examined. Except fur the aqueous extract of Allium bulb, ethanolic leaf extract of all other plants more efficiently reduced the colony growth of the fungus on potato-dextrose-agar, Of which, Allium and Eucalyptus extracts were more effective. Initiation of radial growth of M. roridum on solid media was deferred maximum 6 days by ethanolic Eucalyptus extract and 4 days by aqueous Allium extract at $0.4 mg.ml^{-1}$. In the liquid media amended with Eucalyptus extract ($0.4 mg.ml^{-1}$) complete inhibition of sporulation was noticed upto 8 days, and initial inhibition of mycelial bio-mass generation was considerably diminished with time and reduction was 1.3 fold 14 days after application. While, complete inhibition of mycelial growth for 6-14 days was recorded with $\geq$0.1 mg.ml$^{-1}$ commercial eucalyptus oil. However, rejuvenation of growth appeared when fungus was re-inoculated in fresh media. Post-inoculate application of different doses Of Eucalyptus and Allium extracts significantly (p < 0.05) reduced the disease severity in pot-ted mulberry. However, persistence of the effect up to 28 days was apparent at $\geq$ 1.0 mg.ml$^{-1}$ and effectively was on par with carbendazim (1 mg.ml$^{-1}$ ). Almost equal control ability of 1.0 mg.ml$^{-1}$ Eucalyptus extracts can be achieved by ca. 10 times lowered dose of commercial eucalyptus oil. It seems, the toxic principle of E. citrodora to M. roridum is fungistatic in nature and may have essential oil based origin.

An Air Cleaning Efficiencies of Wet Air Cleaner in the Swine Finishing Winch Curtain Stall (윈치커튼식 비육돈사에서 습식공기정화기의 공기정화 효율 분석)

  • Oh, I.H.;Kim, W.G.;Lee, H.S.
    • Journal of Animal Environmental Science
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    • v.14 no.2
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    • pp.75-80
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    • 2008
  • High concentration of $NH_3$, $CO_2$, and lots of dust are found in modern densely raising stall system, as results, they provide a negative influence on animal and lamer health, and production ability. Therefore, it is necessary to keep clean the inside of stall air to increase the productivity. A wet type air cleaner has been developed to clean the stall air. The work principle is that the inside air are sucked through the fan, and the rotating discs make a water into a fineness spray and blows into the stall. The spray can take the dust, $NH_3$, and odor from the stall inside air and give back to the circulating water, which can be refreshed in 2 hours interval. In the Present study, we measured the $NH_3$, dust, odor, temperature and humidity in a swine stall that were installed two wet air cleaners with 700 fattening swine with on-mode and off-mode of wet air cleaners. In fall, the concentrations of $NH_3$ in off-mode stall were maximum 24 ppm and minimum 16 prm, and the average was 18.2 ppm. However in on-mode stall the $NH_3$ concentrations were maximum 7ppm and minimum 1ppm, and the average was 2.7ppm. The concentration of $NH_3$ in on-mode was 74% lower than off-mode stall. Odor was measured by olfactometer. In the off-mode stall, the odor unit was 3,800 OU/$m^3$, but in the on-mode stall the odor unit was 2,100 OU/$m^3$ Odor removal efficiency was about 45% in on-mode stall. The dust measure was divided into 3 categories, $PM_{10}$, $PM_{2.5}$ and $PM_{1.0}$. Whereas the $PM_{10}$ showed no significant differences between the tests, $PM_{2.5}$ and $PM_{1.0}$ in the fine particle range reduced remarkably in the on-mode.

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Performance Testing of an Integrated Hybrid Actuator (집적형 하이브리드 구동장치의 성능시험)

  • Xuan, Zhefeng;Jin, Tailie;Goo, Nam Seo;Bae, Byung-Woon;Kim, Tae-Heun;Ko, Han Seo;Yoon, Ki-Won
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.41 no.1
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    • pp.25-30
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    • 2013
  • The piezoelectric-based hydraulic actuator is a hybrid device consisting of a hydraulic pump driven by piezoelectric stacks that is coupled to a conventional hydraulic cylinder via a set of fast-acting valves. Nowadays, such hybrid actuators are being researched and developed actively in many developed countries by requirement of high performance and compact flight system. In this research, operation principle and performance testing of the hybrid actuator were introduced. Output velocities have been measured in both loaded case and not loaded case and the blocking force also has been measured in external loaded case. The maximum velocity of the actuator is 53.3 mm/s, blocking force is 240.7 N and corresponding power output is 3.2 W.

Design consideration and explosion safety of underground ammunition storage facilities (지하탄약고의 설계요소 및 폭발안전 연구)

  • Kim, Oon-Young;Lee, Myung-Jae;Kim, Min-Seok;Kim, Joon-Youp;Joo, Hyo-Joon
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.5 no.1
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    • pp.55-70
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    • 2003
  • Ammunition magazine, which is installed on the ground, has difficulty in protecting from the external attack, and accidental explosion should cause great damage to the life and property. For these reasons, it is needed to develop underground magazine that it has the advantages of safety, security and maintenance. This paper introduce the design case for blasting facilities, which should resist blasting pressure, as well as layout of underground magazine, which takes a safety for explosion and a working space of loading/unloading machine into consideration. On the layout, in case of ${\bigcirc}{\bigcirc}$ underground magazine, put three storage chambers in position almost parallel with principle stress direction, where less effected on discontinuity and hard rock area. Also, secured safe distance according to safety criteria of the Defense Ministry, and verified suitable layout by trace simulation for loading/unloading machine on working stage. Blasting design was performed on evaluation of maximum blast pressure between donar and acceptor chambers, and design condition for blast door, valve, etc. Diminution facilities against explosion, such as thrust block or debris trap, determined its size after plan in accordance with blasting criteria and calculation by structural analysis.

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