• 제목/요약/키워드: the inducible

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Heat Inducible Expression of the CDC70 Gene Under the Control of Heat Shock Element in Saccharomyces Cerevisiae

  • Lee, Seok-Jae;Jahng, Kwang-Yeop;Lee, Young-Hoon;Chae, Keon-Sang
    • Journal of Microbiology
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    • 제33권3호
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    • pp.196-200
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    • 1995

  • In order to express the CDC70 gene of Saccharomyces cerevisiae by heat shock, we have designed heat inducibe hybrid promoters using the Drosophila melanogaster heat shock elements (HSEs). A 220 bp-long upstream fragment of the D. melanogaster hsp70 gene comprised of four HSEs was placed upstream of the putative proximal TATA box of the CDC70 gene. Hybrid promoters containing different fusion joints were tested for their ability to drive the CDC70 gene expression by heat shock. The results showed that the HSEs of D. melanogaster conferred the heat-induced CDC70 gene expression, but the heat inducibility was much lower than that in D. melanogaster.

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Carrageenin으로 흰쥐 발 염증으로 Indomethacin에 의한 유도성 nitric oxide synthase의 발현증가 (Potentiation of Inducible Nitric Oxide Expression by Indomethacin in Carageenin-treated Rat Paw Inflammation)

  • 원혜영;강건욱;김영미;김낙두
    • 약학회지
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    • 제43권2호
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    • pp.214-220
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    • 1999

  • Present study was aimed to examine whether indomethacin affected the production of NO in the rat paw exudate by carrageenin. Paw edema and nitrite/nitrate levels in the paw exudate were maximal after 4 h and remained elevated up to 10 h, whereas indomethacin (10 mg/kg, po) significantly inhibited the carrageenin-induced paw edema and levels of nitrate in the paw exudate. However, paw edema and nitrite/nitrite levels were increased thereafter for 10 h. Indomethacin also enhanced the expression of iNOS mRNA and protein 4 h after carrageenin infection. Indomethacin inhibited the level of $PGE_2$ in the paw exudate in a time-dependent manner. These results suggest the possibility that indomethacin may potentiate expression of iNOS and subsequently increase nitrite/nitrate level in the late phase of carrageenin-induced rat paw inflammation possibly by suppressing cycloxygenase activity.

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