• Journal of Microbiology and Biotechnology
• /
• 제30권8호
• /
• pp.1261-1271
• /
• 2020

Cytochrome c_L (Cytc_L) is an essential protein in the process of methanol oxidation in methylotrophs. It receives an electron from the pyrroloquinoline quinone (PQQ) cofactor of methanol dehydrogenase (MDH) to produce formaldehyde. The direct electron transfer mechanism between Cytc_L and MDH remains unknown due to the lack of structural information. To help gain a better understanding of the mechanism, we determined the first crystal structure of heme c containing Cytc_L from the aquatic methylotrophic bacterium Methylophaga aminisulfidivorans MP^T at 2.13 Å resolution. The crystal structure of Ma-Cytc_L revealed its unique features compared to those of the terrestrial homologues. Apart from Fe in heme, three additional metal ion binding sites for Na⁺, Ca⁺, and Fe²⁺ were found, wherein the ions mostly formed coordination bonds with the amino acid residues on the loop (G93-Y111) that interacts with heme. Therefore, these ions seemed to enhance the stability of heme insertion by increasing the loop's steadiness. The basic N-terminal end, together with helix α4 and loop (G126 to Y136), contributed positive charge to the region. In contrast, the acidic C-terminal end provided a negatively charged surface, yielding several electrostatic contact points with partner proteins for electron transfer. These exceptional features of Ma-Cytc_L, along with the structural information of MDH, led us to hypothesize the need for an adapter protein bridging MDH to Cytc_L within appropriate proximity for electron transfer. With this knowledge in mind, the methanol oxidation complex reconstitution in vitro could be utilized to produce metabolic intermediates at the industry level.

• Journal of Mechanical Science and Technology
• /
• 제17권4호
• /
• pp.501-510
• /
• 2003

The spot welded sections of automobiles such as the hat and double hat section members, absorb the most of the energy during the front-end collision. The purpose of this study was to analyze the collapse characteristics of spot welded section members with respect ttl the pitch or spot welds on flanges. through impact experiments and computation for para-closed sections and perfectly closed sections. The hat shaped section members were tested at the impact collapse velocities of 4.72 m/sec, 6.54 m/sec and 7.19 m/sec and double hat shaped section members were tested at the impact collapse velocities of 6.54 m/sec, 7.19 m/sec and 7.27 m/sec. A commercial LS-DYNA3D was used to simulate the collapse behavior of the hat and double hat shaped section members. The validity of the simulation was to be proved by comparing the simulation results and the experimental results.

• 통합자연과학논문집
• /
• 제15권2호
• /
• pp.89-97
• /
• 2022

This paper presents the comprehensive feasibility study of solar mini-grid project located in Bajhang District, Sudur Paschim Province, Nepal. The study has been conducted with the aim of developing a suitable size solar mini-grid system to meet electricity demand of proposed settlements of the village people. The study forecasts that the estimated average daily peak power consumption of load is about 20kW and average daily energy demand of load is about 100-150kWh/day in the base year 2022. The shared ratio of productive end uses is about 25% of the total power consumption and about 27% of the total energy demand, which will be used for small business/income generation activities and required 45kWp size solar power generation mini-grid system. The estimated project cost for the proposed 45kW solar mini-grid system technology, including 3 years of operation & maintenance, as well as power distribution network up to end user's premises is about 0.24 million USD. It is concluded that 45kWp photovoltaic mini-grid is feasible for the location.

• Nuclear Engineering and Technology
• /
• 제39권4호
• /
• pp.337-348
• /
• 2007

A fuzzy neural network model is presented to predict residual stress for dissimilar metal welding under various welding conditions. The fuzzy neural network model, which consists of a fuzzy inference system and a neuronal training system, is optimized by a hybrid learning method that combines a genetic algorithm to optimize the membership function parameters and a least squares method to solve the consequent parameters. The data of finite element analysis are divided into four data groups, which are split according to two end-section constraints and two prediction paths. Four fuzzy neural network models were therefore applied to the numerical data obtained from the finite element analysis for the two end-section constraints and the two prediction paths. The fuzzy neural network models were trained with the aid of a data set prepared for training (training data), optimized by means of an optimization data set and verified by means of a test data set that was different (independent) from the training data and the optimization data. The accuracy of fuzzy neural network models is known to be sufficiently accurate for use in an integrity evaluation by predicting the residual stress of dissimilar metal welding zones.

• 대한기계학회논문집A
• /
• 제25권1호
• /
• pp.98-105
• /
• 2001

The fundamental and widely used spot welded sections of automobiles (hat and double hat-shaped section members) absorb most of the energy in a front-end collision. The sections were tested on axial static(10mm/min) and quasi-static(1000mm/min) loads. Based on these test results, specimens with various thickness, shape and spot weld pitch on the flange have been tested with impact velocity(7.19m/sec) the same as a real life car clash. Characteristics of collapse have been reviewed and a structure of optimal energy absorbing capacity is suggested.

• 한국음향학회:학술대회논문집
• /
• 한국음향학회 1998년도 학술발표대회 논문집 제17권 1호
• /
• pp.351.1-355
• /
• 1998

Network-based real-time multimedia applications require guaranteed performances of communication service. Also according to increased bandwidth and processing performance in the future high speed networks, it is possible to make multimedia real-time applications such as distributed real-time applications including video conference, medical imaging, and video education. These applications have characteristics of stringent performance requirements in terms of delay, delay jitter and maximum loss rate. This paper proposes a new delay control for Broadband networks with FCFS queueing. The delay control provides an alternative solution to a current problem in real-time communication; traffic distortion, which makes it difficult to meet such real-time requirements. In simulations, it shows that the proposed delay control can guarantee local and end-to-end delay.

• Structural Engineering and Mechanics
• /
• 제23권5호
• /
• pp.563-577
• /
• 2006

This study involves a series of experiments on the buckling strength of eccentrically compressed cold-formed stainless steel square hollow-section columns. The principal parameters in this study are slenderness ratios ($L_k/r$ = 30, 50, 70) and magnitude of eccentricity e (0, 25, 50, 75, 100 mm) on the symmetrical end-moment. The objectives of this paper are to obtain the buckling loads by conducting a series of experiments and to compare the behavior of the eccentrically compressed cold-formed stainless steel square hollow-section columns with the results of the analysis. The ultimate buckling strength of the square-section members were determined with the use of a numerical method in accordance with the bending moment-axial force (M-P) interaction curves. The behavior of each specimen was displayed in the form of a moment-radian (M-${\theta}$) relationship. The numerically obtained ultimate-buckling interaction curves of the beam columns coincided with the results of the experiments.

• 대한가정학회지
• /
• 제36권3호
• /
• pp.77-86
• /
• 1998

The aims of this study show the purpose of writing[Ka-ryae] and its transmission and operation from the end of Koryo to Chosun dynasty. And Studying of a traditional marriage ceremony as shown in [Ka-ryae], We intend to find a mental meaning and value in it. The major findings this study are as follows. 1. Though [Ka-ryae] was written by Chu-Ja who lived in the period of South-Song of China, it had greatly influenced the thought, system and life of Korean society from the end of Koryo to Chosun dynasty. 2. Studying the content of [Ka-ryae], we learned the respect, discretion and reghtness as the mental meaning and value of a marriage ceremony.

• 한국수학사학회지
• /
• 제11권1호
• /
• pp.1-9
• /
• 1998

This article explores what is the genuine Koreanness in Korean arithmetic by examining what kind of influence the Chinese arithmetic had on the Korean arithmetic and how the Korean arithmetic scholars had accepted and utilized it. Because the main stream of Korean culture before the end of Chosun dynasty was located under the umbrella of the Chinese philosophy, technique, and culture, it is necessary to make researches on the historical documents and materials in order to establish the milestone in the Korean arithmetic history for the Korean arithmetic scholars. For this research, the arithmetic books published in between the sixteenth century and the end of Chosun dynasty are mainly consulted and discussed, dealing with the bibliographical introduction in the arithmetic Part in Re Outline History of the Korean Science & Technology written by Prof. Yong-Woon Kim.

• 한국정밀공학회지
• /
• 제27권8호
• /
• pp.54-61
• /
• 2010

In this research KP-4, one of the plastic mold steels, was coated with the AlTiN from one layer to four layers by the PVD method in the $\Phi$ cemented carbide ball end mill. Coated KP-4 was processed with various conditions. For example, slope of $15^{\circ}$, $30^{\circ}$ and $45^{\circ}$ the spindle rotation speed was changed from 10,000rpm to 16,000rpm, the tool feeding speed was changed from 1,300mm/min to 1,700mm/min, the depth of cut was also changed from 0.3mm to 0.9mm, and etc. Cutting component force according to the coating layer number, surface roughness, and the wear of tool were studied.