• Biomedical Science Letters
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• v.28 no.4
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• pp.327-333
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• 2022

The DNA profiling of short tandem repeat (STR) markers is a powerful tool for forensic identification and forensic paternity testing. However, STR loci are susceptible to mutation that cause mismatches between parents and children when paternity is tested. Herein, we examined paternity disputes with 23 autosomal STR loci using two commercial human identification kits and revealed successive mismatches at the D13S317 locus across three generations of a Korean family. Additionally, we investigated 12 X-chromosomal STRs and discovered an inconsistency at the DXS10148 locus between the father and daughter of the same Korean family. Furthermore, we confirmed STR genotypes at the D13S317 and DXS10148 loci of the family using sequencing analysis. Consequently, we identified a successive single-step mutation at the D13S317 locus and one single-step mutation at the DXS10148 locus in three generations of the Korean family. Therefore, this case study may be useful for interpreting and understanding forensic paternity tests.

• Annals of Clinical Neurophysiology
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• v.8 no.1
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• pp.23-28
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• 2006

Background: central core disease is one of the non-progressive benign congenital myopathies characterized by the presence of cores in muscle fibers, which was originally described by Shy and Magee (1956). We describe clinical charcteristics of central core disease in a Korean family manifested by autosomal dominant pattern through three generations. Methods: Clinical, serologic, and electrophysiologic profiles were evaluated in eleven members among 22 family members through three generations. Results: Six family members were symptomatic and five were non-symptomatic. Instead of proximal muscle weakness, musculoskeletal manifestations including non-specific joint pain and stiff sense were the most frequent symptoms. Muscle biopsy performed in two symptomatic patients revealed that type I fiber showed central halo, which is charactreristics of central core disease. No remarkable findings were present in serologic study including CPK level and electromyographic findings suggesting myopathic pattern were only present in two patients among 11 symptomatic group. Conclusions: In evaluating non-specific musculoskeletal complaints from the familial members showing genetic trait, central core disease should be considered to one of the possible diagnosis.

• Journal of Community Nutrition
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• v.5 no.2
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• pp.93-104
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• 2003

Although a generation-related difference in nutrient and food-group intake has been broadly recognized in the Japanese population, few studies have examined the difference and correlation of intakes with consideration of a family line and co-habitation status. We conducted a dietary survey using female college students in dietetic course in Aichi, Japan, and their mothers and grandmothers. A validated self-administered diet history questionnaire was used for assessment of intakes. Data from 110 families were included in the analysis. The means of 13 nutrients and 10 food groups (of the 15 and 14, respectively) showed significant differences among the three generations. A significant difference was observed in the carbohydrate, protein, saturated fatty acid, cholesterol, and fish intakes between the students living with their mothers and those apart. In the correlation analyses between generations, moderate correlation was observed for most nutrients and food groups between the students and their mothers living together (correlation range = 0.30-0.61 for nutrients, and = 0.21 -0.56 for food groups). A wide variation with no consistency was observed for the correlation between the students and their grandmothers (-0.18 -0.59 and -0.33 -0.65, respectively), No meaningful correlation was observed between any two generations living apart. Among the food groups examined, pulses, fish, and vegetables showed relatively large differences for the correlation between two groups with different living conditions. When living together, the correlation coefficients for nutrients and food groups between the students and their mothers decreased according to the increase in frequency of eating out by the students. These results suggest that living together and eating together were an important factor for the resemblance in dietary habits between generations in the population with a marked generation-related difference in intakes. (J Community Nutrition 5(2) : 93-104, 2003)

• Journal of Families and Better Life
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• v.27 no.1
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• pp.197-208
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• 2009

The purpose of this study is to examine the intergenerational transmission of mother-daughter attachment across three generations, and to determine whether the mother-daughter attachment of three generations influences the adult daughter's ego-resilience. The subjects of this study were 310 unmarried adult women aged 20${\sim}$29, residing in the Busan area, and their 310 middle-aged mothers. The mothers responded to two sets of questionnaire investigating their attachment to their mothers (G1-G2) in the past and the present attachment to their adult daughters (G2-G3). Meanwhile, the adult daughters were given questionnaires regarding their attachment to their mothers and their ego-resiliency. The measurements used for this study were the Inventory of Parent and Peer Attachment (IPPA) and the Ego-Resiliency Scale (ER). Among the distributed questionnaires, 265 sets were collected and 252 sets were actually analyzed using SPSS 12.0 after 13 sets had been excluded due to incomplete data. Basic statistics were used such as frequency analysis, Pearson's correlation and hierarchical regression analysis. The study results were as follows. First, the adult daughter-mother (G2-G3) attachment was explained mostly by mother-grandmother (G1-G2) attachment, daughter's age, and economic status of the family. The strongest factor was the mother-grandmother attachment which implies the transmission of attachment through generations. Second, among the factors that influenced the ego-resilience of an unmarried adult daughter, attachment to one's mother perceived by the daughter turned out to be the most significant. Especially, the more positive the adult daughter's emotion toward her mother and the higher the daughter's education, the stronger the ego-resilience of the adult single daughter was. It was concluded that the mother-daughter attachment remained consistent throughout three generations, which influenced the social-psychological adjustment of the adult unmarried daughter.

• Proceedings of KHEA Conference
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• 1997.07a
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• pp.327-337
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• 1997

• Plant Resources
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• v.3 no.3
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• pp.184-193
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• 2000

Soybean [Glycine max (L.) Merr.] seed weight is a important trait in cultivar development. Objective of this study was to identify and confirm quantitative trait loci (QTLs) for seed weight variation in the F2 and F2:3 generations. QTLs for seed weight were identified in F2 and F2:3 generations using interval mapping (MapMaker/QTL) and single-factor analysis of variance (ANOVA). In the F2 plant generation (i.e., F3 seed), three markers, OPL9a, OPM7a, and OPAC12 were significantly (P<0.01) associated with seed weight QTLs. In the F2:3 plant row generation (i.e., F4 seed), five markers, OPA9a, OPG19, OPL9b, OPP11, and Sat_085 were significantly (P<0.01) associated with seed weight QTLs. Two markers, OPL9a and OPL9b were significantly (P<0.05) associated with seed weight QTLs in both generations. Two QTLs on USDA soybean linkage group C1 and R were identified in both F2 and F2:3 generations using interval mapping. The linkage group C1 QTL explained 16% of the variation in seed weight in both generations, and the linkage group R QTL explained 39% and 41% of the variation for F2 and F2:3 generation, respectively. The linkage group C2 QTL identified in F2:3 generation explained 14.9% of variation. Linkage groups C1, C2 and R had previously been identified as harbouring seed size QTLs. The consistency of QTLs across generations and populations indicates that marker-assisted selection is possible in a soybean breeding program.

• Journal of Life Science
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• v.7 no.4
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• pp.358-360
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• 1997

Soybean [Glycine max (L.) Merr.] seed size is a important yield component and is a primary consideration in the development of cultivars for specialty markets. Our objective was to examine the consistency of QTLs for seed size across generations. A 68-plant F$_{2} segregation population derived from a mating between Marcury (small seed) and PI 467.468 (large seed) was evaluated with RAPD markers. In the F$_{2} plant generation (i.e. F$_{3} seed), three markers, OPL09a, OPM)7a, and OPAC12 were significantly (P<0.01) associated with seed size QTLs. In the F$_{2} ; F$_{3} generation (i.e., F$_{4} seed), four markers, OPA092, OPG19, OPL09b, and OPP11 were significantly (P<0.01) associated with seed size QTLs. Just two markers, OPL09a, and OPL09b were significantly (P<0.05) associated with seed size QTLs in both generations. The consistency of QTLs across generations indicates that marker-assisted selection for seed size is possible in a soybean breeding program.

• Journal of the Korean housing association
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• v.13 no.3
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• pp.85-91
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• 2002

The purpose of this study is to find out the effective planning guidelines of elderly housing fur the aged people living with their sons or daughters. To investigate the actual conditions and the problems of housing environments, two hundred and six old people in Gwangiu city, aged over sixty and living with their sons and daughters, were interviewed individually and they provided the useful answers to the questions. The houses for three generations need to have the proper degree of privacy fur each family member, by arranging rooms for their own independent living space while securing the sufficient common space. Therefore, independent and safe living environments are to be made for the elderly and the choice of various types be made according to their own demands. It is also necessary to guarantee the continuation of inhabitation by establishing health care systems and life support services.

• Journal of the Korean housing association
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• v.12 no.4
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• pp.93-104
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• 2001

This study has been performed to analyze size and deficit problem of residential space in later life. In this study, 216 Koreans aged 50 to 79 were surveyed for their current residential space ad family related items such as age, marital status, living arrangement, birth order, and housing status. The results are drawn as follows: 1. The space size significantly fluctuates by marital status, number of generations living together, tenure, and structure type; For example, use of indoor area per person ranges from 7.01 pyoung for three-generation households to 19.53 for one-generation households. 2. s for space deficit, very old widowed females, 60's living in rented or multi-family housing units, and those living in three generations are high risk groups of the space deficit below 7 young per person. In this study, further researches are recommended as follows; Regarding the highest risk group of space deficit, life styles causing space-related conflicts should be studied, and accordingly proper welfare services for the causal life styles be offered. Housing maintenance & utility costs be investigated and guidelines should be proposed for any surplus groups among the 60's, so that their space surplus can be reduced by suitable adjustment process.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.2
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• pp.89-94
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• 1998

Granule bound starch synthase (GBSS), also known as the '"waxy protein'", is responsible for the synthesis of amylose in the amyloplasts of cereal crops. In hexaploid wheat (Triticum aestivum L.), GBSS is involved in amylose synthesis and rolls as an important factor to determine flour quality and end-use quality in food products. Genes on three Wx loci have been found to encode GBSS in common wheats. We developed techniques for the purification and separation of GBSS in wheat. Three major GBSS isoforms, which were encoded by the genes on three loci, Wx-A1, Wx-B1, and Wx-D1 migrating differently by one dimensional SDS-po-lyacrylamide gel electrophoresis (1D SDS-PAGE), were identified. GBSS from 66 Korean hard and soft winter wheats were purified and determined for their Wx loci and four of them were identified possessing a null allele either at the Wx-A1 and Wx-B1 loci. With help of identification of three GBSS isoforms using 1D SDS-PAGE system, we are able to identify and monitor Wx gene expressions in breeding materials for developing waxy or partial waxy wheats without experiencing consecutive selecting generations.cting generations.