• World Technopolis Review
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• v.8 no.2
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• pp.92-103
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• 2019

The first generation of Japan's smart city policies began around 2010. However, the latest trends in smart city policies and the impacts of the first generation on the latter one were not fully covered in either official documents or academic literature. In such circumstances, the purposes of this study were firstly to identify outcomes derived from the smart city projects in the first generation, and then, to reveal the present situation of the latest smart city policies, including the influence of the first generation on these state of the art policies. The present study was also intended to evaluate the validity of a conceptual framework presented by Fernandez-Anez et al. (2018) for smart city policies. As a result, it was revealed that (1) policy outputs and outcomes derived from the smart city policies in the first generation were highly regarded, (2) the conceptual framework of smart city policies was evaluated as valid, and (3) the second generation of smart city policies after Society 5.0 was characterized by the establishment of smart city platforms.

• Journal of Embryo Transfer
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• v.10 no.1
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• pp.73-82
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• 1995

large scale production of cloned embryos requires the technology of multiple generation nuclear transplantation(NT) using NT embryos as the subsequent donor nuclei. The purposes of this study were producing the second generation cloned rabbit embryos, and also to determine the electrofusion rate and in vitro developmental potential comparatively in the cloned embryos of the first and second NT generation. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) containing 10% fetal calf serum(FCS) at 47 hours after hCG injection In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gi /S transition of 32-cell stage. The first generation NT embryos which were developed to 8-cell were synchronized in Gi /S transition phase of the following 16-cell stage and used as donor nuclei for second generation Synchronization of the cell cycle of blastomeres was induced, first, using an inhibitor of microtuble polymerization, colcemid for 10 hours to arrest blastomeres in M phase, and secondly, using a DNA synthesis inhibitor, aphidicolin for 1.5 to 2 hours to arrest them in Gi /S transition boundary. The recipient cytoplasms were obtained by removing the nucleus and the first polar body from the oocytes collected at 14 hours after hCG injection. The separated donor blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were electrofused by electrical stimulation of three pulses for 60 $\mu$sec at 1.25 kV /cm in 0.28 M rnannitol solution The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. Following in vitro culture of the first and second generation cloned embryos to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The results obtained were summarized as follows: 1. The electrofusion rate was found to be similar as 79.4 and 91.5% in the first and second generation NT rabbit embryos, respectively. 2. The in vitro developmental potential to blastocyst stage of the second generation NT embryos (23.3%) was found significantly(p<0.05) lower, compared with that of the first generation NT embryos (56.8%). 3. The mean blastomeres counts of embryos developed to blastosyst stage following in vitro culture for 120 hours and also their daily cell cycles during the culture period were decreased significantly (p<0.05) to 104.3 cells and 1.33 cylces in the second NT generation, compoared with 210.4 cells and 1.54 cycles in the first NT generation, respectively.

• Korean Journal of Child Studies
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• v.32 no.4
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• pp.63-81
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• 2011

The purpose of this study was to examine the first generation Korean-American mothers' attachment representation as well as the attachment representation of their second generation Korean-American college students. The subjects consisted of 25 first generation Korean-American mothers and 27 second generation college students residing in Los Angeles. The Adult Attachment Interview (George, Kaplan, & Main, 1985) was used for assessing attachment representation patterns. The results of this study were as follows. First, 36% of the subject mothers were classified as secure, 64% were insecurely attached and there was no unresolved/disorganized pattern in the insecure group. Second, 33% of the subject students were classified as secure. 67% were insecure on the AAI, and there was 4% unresolved/disorganized patterns in the insecure group. Third, a difference of 76% was found between the correspondence between the first generation Korean-American mothers' attachment representation pattern and the attachment representation of the second generation Korean-American college students.

• Journal of Veterinary Clinics
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• v.12 no.1
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• pp.877-886
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• 1995

The recycling nuclear transplantation(NT) technique has the powerful potential of producing a large number of genetically identical embryos and offsprings from one embryo. Multiple generational cloning by this technique utilizes the NT embryo itself as the donor for the next generation of cloning. In this experiment, we have produced the third generational cloned embryos by recycling NT. Further we examined comparatively the electrofusion rate and in vitro developmental potential in the cloned embryos of the first second and third generations. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulberco's phosphate buffered saline containing 10 % fetal calf serum(FCS) at 47 hours after hCG injection. In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gl/S transition of 32-cell stage. The first and second generation NT embryos developed to 16-cell were used as donor nuclei for second and third generation. The recipient cytoplasms were utilized the oocytes collected at 14 hours after hCG injection, following revoming the nucleus and the first polar body by micromanipulation. The separated blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were fused by electrical stimulation. The electrofusion rate was seen to be 78.0, 88.0 and 90.3 % in the first second and third generation NT rabbit embryos, respectively. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10 % FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The in vitro developmental potential to blastocyst stage was significantly(P<0.05) decreased in the third(7.2 %) generation NT embryos compared to the first(53.1 %) and second(16.1 %) generation NT embryos. Following in vitro development to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The mean blastomere numbers and cell cycle numbers of NT embryos during the culture period were significantly(p<0.05) decreased in the second(93.9 cells and 6.55 cylces) and third(81.5 cells and 1.35 cylces) generation, compared to the first(189.9 cells and 7.55 cylces) generation.

• Journal of Fashion Business
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• v.21 no.1
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• pp.12-31
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• 2017

Korean commercial-organized girl groups were remarkable in the late 1990's. However, by the late 2000's, girl groups had an even more profound effect on Korean popular music compare to past influences. This study aimed to analyze the social-cultural meaning of the changing appearance of girl group between the first and second-generations. For this purpose, this study analyzed media image and text, based on a social-cultural context, about 13 girl groups. The results are as follows. First, while the first -generation girl group tended to maintain girlish/sexy images trying to the male desire, the second -generation girl group strategically showed various sexual identities such as femininity, masculinity, masculinity and androgyny along with contextual sexual images. The reason why girl groups increased the number of strategic images featuring various sexual identities was in order to appeal to a wide, diverse audience. Second, the second generation girl groups had - slim bodies with great athleticism, basically due to trainee system. Because of this, their semiotic body images have been commercially used to promote the consumption. Third, the second generation girl groups - were the bigger stars than first generation girl groups - because the members worked in many different fields. Therefore, the group members' images were successful consumed directly and then reproduced symbolically. Fourth, each member of the second -generation girl groups characterized by appearing in diverse, yet familiar images, through various media sources. Although the intention of this was to have recognition and popularity, it became difficult for them to change their image once one particular image was deemed popular.

• Journal of the Korea Safety Management & Science
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• v.10 no.2
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• pp.105-111
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• 2008

Six sigma has been evolved into three generations. The first generation focused on eliminating or reducing defects as Motorola originally developed and applied. The second generation focused on reducing costs and improving process efficiency as GE extended the first generation. The next generation of six sigma such as D2MAIC(Discovery, Define, Measure, Analyze, Improve, Control) and ICRA(Innovate, Configure, Realize, Attenuate) has been discussed since the beginning of the 21st century. Although the third generation of six sigma emphasizes value creation, but there are few specific tools for its implementation. In this thesis, some tools for finding opportunities for value creation are suggested. It is explained and discussed with examples how the tools can be applied.

• American Studies
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• v.42 no.1
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• pp.99-132
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• 2019

Like many other Asian American writers, Jhumpa Lahiri writes stories that capture the experiences of immigrant families in America. What sets The Namesake apart is that Lahiri cleverly uses the names of her characters to shape their individual lives and futures not just as a first or second generation immigrant, but as people who are more than what that labelling connotes. Although the struggle faced by Ashoke and Ashima to hold on and adapt as first generation immigrants is contrasted with the search for identity among second generation immigrants seen primarily through the experiences of their children Gogol and Sonia, Lahiri uses their struggles as an immigrant family to serve as a starting point for each member of the Ganguli family to find their own identities and understandings of who they are as individuals apart from their race, history or cultural heritage so that they may truly be "without borders."

• Korean Journal of Adult Nursing
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• v.21 no.6
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• pp.603-616
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• 2009

Purpose: The purpose of this study was to examine the subjective opinions of first-generation Korean-Americans about survival. Methods: The Q-methodology which provides a method of analyzing the subjectivity of each item was used. The 34 selected Q-statements from each of 38 subjects were classified into a shape of normal distribution using a 9 point scale. The collected data was analyzed using a QUANL/WIN program. Results: Four types of survival attitudes from 38 first-generation Korean-Americans were identified. Type I was a satisfaction type based on belief, Type II was an effort type based on purpose, Type III was an isolation type based on ethnicity, and Type IV was a compromise type based on adaptation. Results of this study indicate that approaches to a minority's survival strategies need to be differentiated taking this typology and various subject characteristics into account. Conclusion: This information may provide a basic understanding for healthcare providers who work with first-generation Korean-Americans.

• Human Ecology Research
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• v.60 no.3
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• pp.377-390
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• 2022

This study identified and compared family values within and across different generations concerning gender roles, attitudes toward diverse families, children, and parental roles. Data were obtained from the 2020 Seoul Family Report Survey. There were 1,949 study subjects, including first- and second-generation baby boomers and individuals in generations X, Y, and Z in Korea. Latent profile analyses were conducted using M-plus. Perceptions and views concerning family values were classified into different types. The main findings were as follows. Two types were identified among first baby boomers, and three were identified among second baby boomers. Most of the first and second baby boomers belonged to the "moderate" type. Two types were detected among those in Generation X. Regarding gender roles, most views of Generation X were slightly progressive, while their other family values were more moderate. Three types were identified among those in Generation Y: "progressive," "moderate," and "very liberal." Generation Z was identified as a "progressive" group. This study provides basic data to explore the direction of the coexistence of generations by identifying and comparing diverse family values within and across generations.

• Preventive Nutrition and Food Science
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• v.3 no.3
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• pp.267-271
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• 1998

To evaluate nutrient intakes of the first generation Korean -Americans in Hawaii, a dietary survey was conducted using a food frquency questionaire which included 139 food items most often consumed among Korean foods and American foods. The questionnaire surveyed 157 first generation Korean-Americans living in Hawaii. The 66.7~81.1% of first generation Korean-Americans in Hawaii were of healthy weight. The mean percentages of calories obtained from their dietary analyses were 61% carbohydrate, 23% fat and 16% protein. The calorie distribution of their diet appeared to be similiar between older women and older men, however it was seen that men consumed a higher percent of calories from fat than women among younger subjects. The mean dietary intakes for first generation Korean-Americans exceeded recommended intakes for protein, thiamin, niacin , vitamin A, vitamin C, folacin , phosphorus and iron , but the intakes of energy, riboflavin, vitamin B6 ,vitamin E, calcium and zinc were lower than the recommended dietary allowance for Americans. Compared with other groups based on age and gender , younger men had significantly(p<0.05) higher intakes of riboflavin, phosohrous, iron and zinc, and lower intake of folacin. Older subjects consumed significantly (p<0.05) less protein, riboflavin, vitamin B6 and zinc than did younger subjects, and most of the first generation Korean-Americans in Hawaii consumed adequate levels of saturated fatty acid . The ratio , however, of polyunsaturated fatty acid to saturated fatty acid in the diet of younger men was about 0.61, much lower than the recommeded ration of 1.0 and also was significantly (p<0.05) lower than that of other subjects. Moreover, cholesterol intakes of younger men were close th the maximum recommeded level of 300mg/day.