• 제목/요약/키워드: terminal segment

검색결과 68건 처리시간 0.027초

A mutational anlaysis of the N-terminal protease of bovine viral diarrhea virus

  • Chon, Seung-ki
    • 대한수의학회지
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    • 제39권4호
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    • pp.772-777
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    • 1999
  • The uncaped genomic RNA of bovine viral diarrhea virus (BVDV) initiates translation by recruitment of eukaryotic translation initiation factors at the internal ribosome entry site (IRES). N-terminal protease ($N^{pro}$) is the first translation product of the open reading frame (ORF). By using the vaccinia virus SP6 RNA polymerase transient expression system, we showed previously that deletion of $N^{pro}$ region reduced translation by 21%. To better understand the biological significance of $N^{pro}$ for translation, we carried out a mutational analysis of the $N^{pro}$ region of BVDV cloned in the intercistronic region of a bicistronic reporter plasmid. We constructed a bicistronic expression vector in which the entire 5 UTR and the mutated $N^{pro}$ region (${\Delta}386-901$, ${\Delta}415-901$ and ${\Delta}657-901$) was cloned between two reporter genes, chloramphenicol acetyltransferase (CAT) and luciferase (LUC). In vivo translation analyses showed that $N^{pro}$ region was dispensible for efficient translation. The results indicate that the $N^{pro}$ region is not essential for BVDV RNA translation and the 3' boundary of BVDV IRES is expanded into $N^{pro}$ region, suggesting that $N^{pro}$ may not play a major role in BVDV replication.

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HAUSAT-2 소형 위성 동물 추적 시스템 탑재체 개발 (DEVELOPMENT OF THE HAUSAT-2 PAYLOAD OF ANIMAL TRACKING SYSTEM)

  • 이정남;이병훈;문병영;장영근
    • 한국우주과학회:학술대회논문집(한국우주과학회보)
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    • 한국우주과학회 2006년도 한국우주과학회보 제15권1호
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    • pp.129-132
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    • 2006
  • 한국항공대학교 우주시스템연구실에서는 나노급 위성인 HAUSAT-2 개발과 더불어 주요 탑재체인 동물 추적 시스템을 직접 연구 개발하고 있다. 동물 추적 시스템은 위성에 탑재되는 동물 추적 시스템 수신기와 동물 추적 송신기, 위성으로부터 수신된 신호를 해석하고 처리하는 지상국으로 구성되어 있다. 본 논문에서는 동물추적시스템의 전반적인 운용개념과 하드웨어 개발에 대하여 기술하며 데이터를 처리하기 위한 소프트웨어 알고리즘 및 도플러 편이(Doppler Shift)를 이용한 동물 위치 추정 알고리즘에 대하여 논한다.

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Genogroup position of aquabirnavirus GC-1 isolated from rockfish Sebastes schiegeli in Korea

  • Joh, Seong-Joon;Lee, Youn-Jeong;Song, Chang-Sun;Kang, Shien-Young;Mo, In-Pil;Heo, Gang-Jun
    • 대한수의학회지
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    • 제48권3호
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    • pp.287-293
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    • 2008
  • The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.

A New Species of the Genus Bryocamptus (Copepoda, Harpacticoida, Canthocamptidae) from Alpine Wetlands at Jeju Island, Korea

  • Lee, Jimin;Chang, Cheon Young
    • Animal Systematics, Evolution and Diversity
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    • 제32권3호
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    • pp.219-229
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    • 2016
  • Material of a Bryocamptus species, formerly reported as B. umiatensis Wilson, 1958 from Sakhalin and South Korea, is here recognized as a distinct new species. Specimens of both sexes were recently obtained from an alpine wetland, Sumeunmulbaengdui, located in the Hallasan National Park, Korea, and newly registered as a Ramsar Wetland in 2015. The new species, B. jejuensis n. sp., differs from the type population of B. umiatensis from Alaska by the smooth margin of the anal operculum in both sexes, the peculiarly modified terminal seta on the distal endopodal segment of the male leg 3, the sword-like spinous seta on the distal endopodal segment of the male leg 4, and the presence of a setule row on the inner distal margin of the caudal rami in the female. Both sexes of the new species are described in detail with particular reference to the male characters. A revised key to the seven species of the genus Bryocamptus Chappuis, 1929 occurring in Korean waters is provided.

솔잎혹파리(Thecodiplosis japonensis Uchida et Inouye)에 관한 연구 II - 성충 생식절의 표면미색구조 - (External Ultrastructure of the Adult Genital Segments of Pine Needle Gall Midge, Thecodiplosis japonensis Uchida et Inouye)

  • 한성식;박용철;이찬용
    • 한국응용곤충학회지
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    • 제27권3호
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    • pp.144-148
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    • 1988
  • 솔잎혹파리(Thecodiplosis japonensis Uchida et Inouye) 성충을 재료로 하여 그 생식절을 주사전자현미경으로 관찰하였다. 웅성 : 배측에서 보면 두쌍의 hypoproct가 있고 그 양쪽에는 한쌍의 claspettes가 있다. 각 부분의 표면은 주로 seta와 sensilla로 덮혀 있다. 특히 gonostylus 상단부에는 특이한 구조가 관찰되었다. 또한 aedeagus가 관찰되었는데 그 끝부분에는 매우 특이한 구조가 나타났다. 자성: Ovipositor는 세개의 마디로 되어 이들 각 마디의 표면구조는 서로 뚜렷한 차이를 보여주었다.

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정지궤도 위성을 이용한 시각동기 지상시스템 시험장비 구축에 관한 연구 (A Study on the Construction of Ground Test Segment for the Time Synchronization System Using the Geostationary Satellite)

  • 이상철;김방엽
    • 한국항공우주학회지
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    • 제32권5호
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    • pp.104-108
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    • 2004
  • CDMA 무선 통신망 기지국의 경우 GPS를 사용한 시각동기 시스템에 전적으로 의존한다. 따라서 우리는 GPS의 예측할 수 없는 고장 또는 미국의 전략적 목적으로, 이용이 불가능할 경우를 대비하여 대체 시스템을 준비해야 한다. 본 연구에서는 정지궤도 통신위성을 이용한 시각동기 시스템을 개발하기 위한 지상 시험 시스템을 구축하였으며, 송수신 보드를 설계, 제작, 시험하였는데, 이 보드는 원자시계로부터 1PPS 신호를 수신 받으면 버퍼에 저장된 데이터를 위성 모뎀에 송신하고, 위성 모뎀으로부터 데이터가 수신되면, Universal Counter 가 지연시간을 측정하도록 1PPS를 생성하여 출력하는 기능을 수행한다.

Copepods of the family Kelleriidae (Crustacea, Copepoda, Cyclopoida) from tropical waters of the Asia-Pacific

  • Hong, Jae-Sang;Kim, Il-Hoi
    • Journal of Species Research
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    • 제10권4호
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    • pp.364-386
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    • 2021
  • Six species of the family Kelleriidae, including four new species, are recorded from tropical waters of the Asia-Pacific, two of them from Phuket Island, Thailand, one each from Bohol Island, Philippines and Phu Quoc Island, Vietnam, and two from Kosrae Island, Micronesia. A new genus Kelleriella is proposed to accomodate one of these new species (Kelleriella quadridens n. sp.). There are three diagnostic features of the new genus: the terminal segment of antenna is armed with one strong claw and six setae, the armature of the maxilliped endopod is reduced, and the third exopodal segment of leg 4 is armed with four spines and five setae. The other three new species are Kelleria latipes n. sp., Kelleria phuketensis n. sp., and Kelleria robusta n. sp. As diagnostic features of these new species, their caudal rami are about 2.5, 3.2, and 1.72 times longer than wide, respectively, the exopod of leg 5 of them is smooth, without any process, about 1.9, 3.7, and 2.9 times longer than wide, respectively, and armed with one spine plus one seta in Kelleria latipes and Kelleria phuketensis or with 2 setae in Kelleria robusta. Additionally, Kelleria javaensis Mulyadi, 2009 is synonymized with Kelleria regalis Gurney, 1927 and Kelleria grandisetiger Kim, 2006 is synonymized with Kelleria andamanensis Sewell, 1949, and both species are redescribed.

Copepods (Cyclopoida) Associated with Compound Ascidians (Tunicata) from Korea, with Descriptions of Nine New Species

  • Lee, Jimin;Kim, Il-Hoi
    • Animal Systematics, Evolution and Diversity
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    • 제38권4호
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    • pp.167-198
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    • 2022
  • Ten species of copepods, including nine new species, associated with compound ascidians are recorded from Korean waters. Nine new species can be characterized by their major diagnostic features, as follows: Botryllophilus pentamerus n. sp. by having a five-annulated abdomen; B. paucisetatus n. sp. by the presence of four and three setae on the exopods of right and left leg 1, respectively; Haplostoma quadridens n. sp. by the presence of four lobes on the labrum and one seta plus four spines on the exopods of legs 1-4; H. paucidens n. sp. by the presence of only two spines on the distal segment of the antenna; Enterocola horridus n. sp. by having five setae on the antenna, no seta on the caudal ramus, and two setae on leg 5; E. longicaudatus n. sp. by having long caudal rami which are more than three times as long as wide; Thoracodelphys bisetata n. sp. by the presence of only two setae on the basis of the maxillule; T. cerasta n. sp. by the presence of a large, horn-like process on the distal margin of the basis of leg 2; and Unimeria hirsuta n. sp. by having three setae on the terminal segment of the maxilla. Zygomolgus didemni (Gotto, 1956) previously known only from European waters is reported from Korean waters, with a redescription and illustrations.

Persistent Trigeminal Artery Variant Detected by Conventional Angiography and Magnetic Resonance Angiography -Incidence and Clinical Significance-

  • Rhee, Sun-Joo;Kim, Myoung-Soo;Lee, Chae-Heuck;Lee, Ghi-Jai
    • Journal of Korean Neurosurgical Society
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    • 제42권6호
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    • pp.446-449
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    • 2007
  • Objective : Persistent trigeminal artery variant (PTAV) is an anastomosis between the internal carotid artery (ICA) and the cerebellar artery without any interposing basilar artery segment. We discuss its probable embryological origin and emphasize clinical implications. Methods : Retrospectively 1250 conventional cerebral angiograms and 2947 cranial magnetic resonance angiographies (MRAs) were evaluated for the patients with PTAV. Results : Five patients (four men and one woman, 23 to 76 years of age, median age 65 years) had a PTAV. Three patients who underwent MRA had a PTAV (3/2947=0.1%). Four of the patients who underwent cerebral angiography had a PTAV (4/1250=0.32%). Two of 143 patients who underwent both conventional angiography and cranial MRA showed PTAV. The PTAV was an incidental finding in all five patients. The PTAV originated from the cavernous segment of the left ICA in four patients and from the cavernous segment of the right ICA in one patient. The terminal branch of the PTAV was the anterior inferior cerebellar artery (AICA) and superior cerebellar artery (SCA) in two patients and the AICA only in the other three patients. Conclusion : Neurosurgeons should be aware of possible presence of PTAV. Manipulation of this vessel during a surgical approach to the parasellar region and percutaneous gasserian ganglion procedure may result in hemorrhage or ischemia.

수근관증후군 진단을 위한 전기생리학적 척도들의 민감도 비교 (The Comparison of Sensitivities of Electrophysiological Parameters for the Diagnosis of Carpal Tunnel Syndrome)

  • 이규택;박수경;유창성;김종규
    • 대한임상검사과학회지
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    • 제37권3호
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    • pp.212-215
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    • 2005
  • Carpal tunnel syndrome (CTS) is the most common entrapment neuropathy in clinical practice, with a 0.1% life time risk in the general population. Conventional neurophysiological studies have been useful in the diagnosis of this condition, as have a number of more specialized procedures. Therefore, we evaluated the diagnostic sensitivities of several parameters in nerve conduction technique for CTS patients. We analyzed 100 patients (159 hands) who were diagnosed with CTS clinically and electrophysiolosically. Median motor and sensory nerve conduction velocities (MCV and SCV) with wrist, palm, and finger stimulation were performed in traditional methods. Sensitivities of each test were calculated and compared to normal control data. The sensitivities of existing nerve conduction method were noted in terminal latency on median nerve, 2nd finger-wrist segment, 3rd finger-wrist segment, palm-wrist segment and distoproximal ratio, as 72.96%, 92.45%, 94.34%, 94.97%, and 97.48%, respectively. In the early course of CTS, sensory nerve conductions in the median nerve are more valuable than motor nerve conduction. Sensory nerve conductions are usually affected before motor nerve conductions in CTS. In this study, we detected that slowing of median SCV was the most frequent in the distoproximal ratio.

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