• Title/Summary/Keyword: tempo

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Metformin enhances the osteogenic activity of rat bone marrow mesenchymal stem cells by inhibiting oxidative stress induced by diabetes mellitus: an in vitro and in vivo study

  • Kai Dong;Wen-Juan Zhou;Zhong-Hao Liu
    • Journal of Periodontal and Implant Science
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    • v.53 no.1
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    • pp.54-68
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    • 2023
  • Purpose: The purpose of this study was to determine whether metformin (MF) could alleviate the expresssion of reactive oxygen species (ROS) and improve the osteogenic ability of bone marrow mesenchymal stem cells derived from diabetic rats (drBMSCs) in vitro, and to evaluate the effect of MF on the ectopic osteogenesis of drBMSCs in a nude mouse model in vivo. Methods: BMSCs were extracted from normal and diabetic rats. In vitro, a cell viability assay (Cell Counting Kit-8), tests of alkaline phosphatase (ALP) activity, and western blot analysis were first used to determine the cell proliferation and osteogenic differentiation of drBMSCs that were subjected to treatment with different concentrations of MF (0, 50, 100, 200, 500 µM). The cells were then divided into 5 groups: (1) normal rat BMSCs (the BMSCs derived from normal rats group), (2) the drBMSCs group, (3) the drBMSCs + Mito-TEMPO (10 µM, ROS scavenger) group, (4) the drBMSCs + MF (200 µM) group, and (5) the drBMSCs + MF (200 µM) + H2O2 (50 µM, ROS activator) group. Intracellular ROS detection, a senescence-associated β-galactosidase assay, ALP staining, alizarin red staining, western blotting, and immunofluorescence assays were performed to determine the effects of MF on oxidative stress and osteogenic differentiation in drBMSCs. In vivo, the effect of MF on the ectopic osteogenesis of drBMSCs was evaluated in a nude mouse model. Results: MF effectively reduced ROS levels in drBMSCs. The cell proliferation, ALP activity, mineral deposition, and osteogenic-related protein expression of drBMSCs were demonstrably higher in the MF-treated group than in the non-MF-treated group. H2O2 inhibited the effects of MF. In addition, ectopic osteogenesis was significantly increased in drBMSCs treated with MF. Conclusions: MF promoted the proliferation and osteogenic differentiation of drBMSCs by inhibiting the oxidative stress induced by diabetes and enhenced the ectopic bone formation of drBMSCs in nude mice.

Integrated Analysis of Visual Story Telling and Original Sound Track of 'Alladin' Animation ('알라딘' 애니메이션에서 비주얼 스토리텔링과 오리지널 사운드 트랙 융합 분석)

  • Jang, So Eun;Lou, Liang;Kim, Jae Ho
    • Korea Science and Art Forum
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    • v.24
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    • pp.375-388
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    • 2016
  • Integrated analysis of OST and VST of animation Alladin is carried out in this study. The OST is classified into 4 stages (Introduction, Bridge Passage, Climax, and Ending) and their OST and VST characteristics are found for each step. Especially, high dynamic characteristics of OST elements (sound, tonality, tempo, major range, intensity, and instrumentation) and VST elements (image structure, camera shot, camera movement) are found in the Climax stage. Full Shot is highly used up to 47.9% and this helps to show that the two characters become one in the completion stage of love. This is common factor shown in the previous analysis of OST "Beauty and the Beast". It is also found that specific musical instruments are matched to specific characters in "Beauty and the Beast" and high/low position or up/down movement of the object in the screen are matched to specific musical instruments in 'Alladin'.

TRAO KSP TIMES: Homogeneous, High-sensitivity, Multi-transition Spectral Maps toward the Orion A and Ophiuchus Cloud with a High-velocity Resolution.

  • Yun, Hyeong-Sik;Lee, Jeong-Eun;Choi, Yunhee;Evans, Neal J. II;Offner, Stella S.R.;Heyer, Mark H.;Lee, Yong-Hee;Baek, Giseon;Choi, Minho;Kang, Hyunwoo;Cho, Jungyeon;Lee, Seokho;Tatematsu, Ken'ichi;Gaches, Brandt A.L.;Yang, Yao-Lun;Chen, How-Huan;Lee, Youngung;Jung, Jae Hoon;Lee, Changhoon
    • The Bulletin of The Korean Astronomical Society
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    • v.44 no.2
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    • pp.68.1-68.1
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    • 2019
  • Turbulence plays a crucial role in controlling star formation as it produces density fluctuation as well as non-thermal pressure against gravity. Therefore, turbulence controls the mode and tempo of star formation. However, despite a plenty of previous studies, the properties of turbulence remain poorly understood. As part of the Taeduk Radio Astronomy Observatory (TRAO) Key Science Program (KSP), "mapping Turbulent properties In star-forming MolEcular clouds down to the Sonic scale (TIMES; PI: Jeong-Eun Lee)", we mapped the Orion A and the Ophiuchus clouds, in three sets of lines (13CO 1-0/C18O 1-0, HCN 1-0/HCO+ 1-0, and CS 2-1/N2H+ 1-0) with a high-velocity resolution (~0.1 km/s) using the TRAO 14-m telescope. The mean Trms for the observed maps are less than 0.25 K, and all these maps show uniform Trms values throughout the observed area. These homogeneous and high signal-to-noise ratio data provide the best chance to probe the nature of turbulence in two different star-forming clouds, the Orion A and Ophiuchus clouds. We present comparisons between the line intensities of different molecular tracers as well as the results of a Principal Component Analysis (PCA).

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L'identità e l'ibridità oltre il confine della Mitteleuropa danubiana in Danubio di Claudio Magris (클라우디오 마그리스의 『다뉴브강』에 나타난 다뉴브강 중부유럽의 탈경계적인 정체성과 혼종성)

  • Lee, Seung Su
    • Lettere Italiane
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    • no.36
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    • pp.73-97
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    • 2012
  • Danubio è un 'diario di viaggio' dalle sorgenti del fiume fino al Mare Nero, attraverso città importantissime o paesi, ampi paesaggi, popoli, costumi, letterature e lingue diverse. Un itinerario fra romanzo e saggio che racconta la cultura come esperienza esistenziale e ricostruisce a mosaico le civiltà di Mitteleuropa rintracciandone il profilo nei segni della grande storia e nelle effimere tracce della vita quotidiana. L'idea di Mitteleuropa nasce a meta dell'Ottocento per indicare un spazio politico e soprattutto economico egemonizzato dai tedeschi e dagli ungheresi, che piu tardi diventa il simbolo di programmi nazionalisti tedeschi e che poi diventa una dimensione sovranazionale, qualcoca di comune e sottostante a tutte le diverse nazionalità e culture di tante realtà diverse. La Mitteleuropa 'hinternazionale', oggi idealizzata quale armonia di popoli diversi, è stata una realtà dell'impero absburgico, nella sua ultima stagione, una tolleranzte convivenza comprensibiilmente rimpianta dopo la sua fine, anche per il confronto con la barbarie totalitaria che le è succeduta, fra le due guerre mondiali, nello spazio danubiano. L'arte absburgica di governo non vuole imporre una rigida unità ai vari popoli, bensi lasciarli sussistere e convivere nella loro eterogeneità. Secondo Claudio Magris, il Danubio è il simbolo della frontiera, perché il Danubio è un fiume che passa attraverso tante frontiere, è quindi simbolo della necessità e della difficoltà di attraversare frontiere, non soltanto nazionali, politiche, sociali, ma anche psicologiche, culturali, religiose. La nostra identità è sempre fragile e noi dobbiamo accettare questa fragilità, poiché mutiamo nel tempo. L'identità fatta di mescolanze, di sottrazioni e di elisioni non fosse soltanto il destino degli epigoni danubiani, bensì una condizione storica generale, l'esistenza di ogni individuo. Magris dice che siccome nessun popolo, nessuna cultra come nessun individuo sono privi di colpe storiche, rendersi impietosamente conto dei difetti e delle oscurità di tutti, e di se stessi, può essere una proficua premessa di convivenza civile e tollerante. Magris dice che la letteratura è di perse stessa una frontiera, una soglia, una zona sul limitare e insegna a varcare i limiti, ma consiste nel tracciare dei limiti, senza i quali non puo esistere e nemmeno la tensione a superarli per raggiungere qualcosa di piu alto e di piú umano.

L'idea dell'umanità nuova nelle tragedie Saul e Mirra di Alfieri (알피에리의 비극 『사울Saul』과 『미라Mirra』에 나타난 새로운 인간의 이상)

  • Jang, Ji Yeon
    • Lettere Italiane
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    • no.36
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    • pp.125-150
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    • 2012
  • Vittorio Alfieri fu celebrato dagli uomini del Risorgimento come "l'iniziatore ed il padre della rinascita" e il creatore della coscienza nazionale. Per questo è lodato da Foscolo, Manzoni, Leopardi, Mazzini, Gioberti, Oriani, Carducci. Ma insieme con i suoi meriti politici, anche i valori artistici delle sue opere non debbono essere dimenticati. Anche sul pensiero e sull'opera dell'Alfieri influirono le ideologie tipiche del secondo Settecento, l'Illuminismo e il nascente Romanticismo, in lui intrecciandosi e scontrandosi ad un tempo. Il suo interesse per lo studio dell'uomo, per la concezione meccanicistica del mondo, la lontananza dalla religione per l'assoluta libertà e l'avversione verso il dispotismo, collegano Alfieri alla dottrina illuminista. I temi letterari illuministici, volti a chiarificare le coscienze e ad apportare il progresso sociale e civile, sono affrontati dal poeta non in modo distaccato, ma con l'emotività e le inquietudini del pensiero romantico. Ma i letterati romantici dell'Ottocento furono piuttosto ostili all'opera dell'Alfieri, accusandola di astratezza, di lontananza dagli interessi e dalla realtà della vita moderna e di assenza di senso storico. Invece nei primi anni del Novecento, Croce lo presenta come iniziatore della nuova letteratura italiana, con la quale egli avrebbe in comune l'individualismo, la passionalità, l'amore per la solitudine, la malinconia. Anche se gli manca l'ansia religiosa sul fine e il valore della vita e l'interessamento per la storia, ecc., secondo il Croce si può definire un protoromantico. Nelle sue opere ci sono, sotto l'aspetto dell'Illuminismo l'odio istintivo contro la tirannide e l'amore per la libertà, e sotto quello protoromantico esplode l'urto violento di sentimenti e passioni e l'esaltazione della morte eroica dei protagonisti. E anche Saul e Mirra descrivono l'idea dell'umanità eroica che muore dopo una lunga vicenda di miseria e di abbiezione. L'idea dell'umanità nuova in Saul e Mirra è l'idea degli uomini nuovi che egli desiderava.

Evaluation of Suitability of Retroreflective Material Locations by Motion Analysis of the Wearer of Safety Clothing (안전의복 착용자의 동작분석에 의한 재귀반사 소재 위치의 적합성 평가)

  • Soon Ja Park;Satoko Tanabe;Mariko Sato
    • Human Ecology Research
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    • v.61 no.3
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    • pp.493-503
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    • 2023
  • Since ISO 20471 regulations(2013) were enacted, high-visibility clothing made of fluorescent fabric and retroreflective materials has been standardized to distinguish the wearers from the surrounding background in a dark place and to protect them. Accordingly, we made high-visibility safety clothing based on the international standards, and examined the positions of the retro-reflective materials on the attached sites to evaluate the suitability of the positions, by 3 types of safety clothing and the body regions when the wearers work or move. Two retro-reflective films of 5cm-horizontal lines were attached to the front/back of the upper-wear, and the lower-wear. Vertical lines were attached from the shoulder to the horizontal waistline. To analyze the motion in 3-dimension, we took pictured infrared-reflective markers on the retro-reflective film covered with yellow-tape on the front-side when 6 subjects wearing experimental garments performed 6 types of motions. According to the metronome tempo, the motions were performed for 30 seconds and repeated 6 motions ①~⑥ by 3 experimental clothing. Among the 6 motions, significant differences in the appearance rate of the markers by body region during the motions were found in 5 types except for motion ③. Significant differences by the post-hoc test were shown in motions ④ and ⑥ as well. Therefore, for high-visibility safety clothing, it is necessary to anticipate the worker's motion and consider the attaching position of the retro-reflective material. It is considered desirable to attach the retro-reflective materials to several places where the clothing is hard to wrinkle.

The Structural Analysis and Criticism of Geommu (Korean Sword Dance) - Focusing in Literary Works and Music - (검무 구조 분석 시론 - 문헌과 음악을 중심으로 -)

  • Kim, Young-Hee
    • (The) Research of the performance art and culture
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    • no.34
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    • pp.9-42
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    • 2017
  • Of all Geommu(劍舞, Korean sword dance), Gisaeng-Geommu danced by gisaeng(妓生, Korean female entertainers) for private guests and at the royal court. The Sword dance in the late Joseon Dynasty used to be dynamic exuding menacing "sword spirit(劍氣)." Sword dance being transmitted today is more ritualistic and elegant. This study considers Korean sword dance has a core structure and motifs transcending generational differences, and based on this critical thinking, aims to analyze the structure of Korean sword dance. This study analyzed the prose "Geommugi(劍舞記)" by Park Je-ga(朴齊家) and the poem "Mugeompyeonjeongmiin(舞劍篇贈美人)" by Jeong Yak-yong(丁若鏞) out of literary works from the late Joseon Dynasty, and from official records of rituals(笏記), "Geomgimu(Sword Dance, 劍器舞)" and "Geommu" in "Gyobanggayo(敎坊歌謠)." In the introduction part of Sword dance, a dancer appears, bows and performs a hand dance or hansam(汗衫) dance to and fro. In the development part, a dancer meets with a sword but first hesitates to hold it and dances holding swords in both hands. The climax shows expert sword skills and combat scenes. In the conclusion part, the court dance involves a dancer bidding a formal farewell, while the dance for entertainment, a dance throws away the sword to finish. From literature materials, the structure of Korean sword dance could be divided into an introduction, a development, a climax and a conclusion. Based on this, this study analyzed sword dance movements by linking the beats accompanying the current sword dance, in the order of a Yeombul, the traditional Korean ballad Taryeong or Neujeun Taryeong, Jajin Taryeong, Taryeong and Jajin Taryeong. The introduction part includes a Buddhist prayer and the beginning of Taryeong. Dancers appear, and in two rows they dance facing each other. On the slow beat, their dances are relaxed and elegant. The development part is matched with Jajin Taryeong. Dancers sit in front of swords and grab them, and they dance holding a pair of swords. The beat gradually becomes faster, progressing the development of the dance. But then, the slower Taryeong is placed again. The reason behind it is to create a tension for a little while, before effectively reaching a climax by speeding up the tempo again. Moving on to Jajin Taryeong, dancers' movements are bigger and more dynamic. The highly elated Jajin Taryeong shows dance movements at the climax on fast, robust beats. In the conclusion part, the beat is quick-tempo and on the upbeat again on Jajin Taryeong. Driving on without a stop on the exciting Yeonpungdae(燕風臺) melody, dancers standing in a line dance wielding the swords and bow before finishing.

Kisspeptin-10 Enhanced Egg Production in Quails Associated with the Increase of Triglyceride Synthesis in Liver

  • Wu, J.;Fu, W.;Huang, Y.;Ni, Y.;Zhao, R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.8
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    • pp.1080-1088
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    • 2013
  • Our previous results showed that kisspeptin-10 (Kp-10) injections via intraperitoneal (i.p.) once daily for three weeks notably promoted the egg laying rate in quails. In order to investigate the mechanism behind the effects of Kp-10 on enhancing the egg laying rate in birds, this study focused on the alternations of lipids synthesis in liver after Kp-10 injections. 75 female quails (22 d of age) were allocated to three groups randomly, and subjected to 0 (control, Con), 10 nmol (low dosage, L) and 100 nmol (high dosage, H) Kp-10 injections via i.p. once daily for three weeks, respectively. At d 52, quails were sacrificed and sampled for further analyses. Serum $E_2$ concentration was increased by Kp-10 injections, and reached statistical significance in H group. Serum triglyceride (TG) concentrations were increased by 46.7% in L group and 36.8% in H group, respectively, but did not reach statistical significance, and TG contents in liver were significantly elevated by Kp-10 injections in a dose-dependent manner. Serum total cholesterol (Tch) concentrations significantly decreased in H group, while in H group the hepatic Tch content was markedly increased. The level of non-esterified fatty acid (NEFA), apolipoprotein A1 and B (apoA1 and apoB) were not altered by Kp-10 injections. The genes expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthetase (FAS), apolipoprotein VLDL-II (apoVLDL-II), cholesterol $7{\alpha}$-hydroxylase (CYP7A1) and vitellogenin II (VTG-II) were significantly up-regulated by high but not low dosage of Kp-10 injection compared to the control group. However, the expression of SREBP-2, acetyl-CoA carboxylase ($ACC_{\alpha}$), malic enzyme (ME), stearoyl-CoA (${\Delta}9$) desaturase 1 (SCD1), apolipoprotein A1 (apoA1), fatty acid binding protein 2 (FABP2), 3-hydroxyl-3-methyl glutaryl-coenzyme A reductases (HMGCR), estrogen receptor ${\alpha}$, ${\beta}$($ER{\alpha}$ and ${\beta}$) mRNA were not affected by Kp-10 treatment. In line with hepatic mRNA abundance, hepatic SREBP1 protein content was significantly higher in H group. Although the mRNA expression was not altered, the content of $ER{\alpha}$ protein in liver was also significantly increased in H group. However, SREBP-2 protein content in liver was not changed by Kp-10 treatment. In conclusion, exogenous Kp-10 consecutive injections during juvenile stage significantly advanced the tempo of egg laying in quails, which was associated with the significant elevation in hepatic lipids synthesis and transport.

Forecasting Brown Planthopper Infestation in Korea using Statistical Models based on Climatic tele-connections (기후 원격상관 기반 통계모형을 활용한 국내 벼멸구 발생 예측)

  • Kim, Kwang-Hyung;Cho, Jeapil;Lee, Yong-Hwan
    • Korean journal of applied entomology
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    • v.55 no.2
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    • pp.139-148
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    • 2016
  • A seasonal outlook for crop insect pests is most valuable when it provides accurate information for timely management decisions. In this study, we investigated probable tele-connections between climatic phenomena and pest infestations in Korea using a statistical method. A rice insect pest, brown planthopper (BPH), was selected because of its migration characteristics, which fits well with the concept of our statistical modelling - utilizing a long-term, multi-regional influence of selected climatic phenomena to predict a dominant biological event at certain time and place. Variables of the seasonal climate forecast from 10 climate models were used as a predictor, and annual infestation area for BPH as a predictand in the statistical analyses. The Moving Window Regression model showed high correlation between the national infestation trends of BPH in South Korea and selected tempo-spatial climatic variables along with its sequential migration path. Overall, the statistical models developed in this study showed a promising predictability for BPH infestation in Korea, although the dynamical relationships between the infestation and selected climatic phenomena need to be further elucidated.

Protein kinase C beta II upregulates intercellular adhesion molecule-1 via mitochondrial activation in cultured endothelial cells

  • Joo, Hee Kyoung;Lee, Yu Ran;Choi, Sunga;Park, Myoung Soo;Kang, Gun;Kim, Cuk-Seong;Jeon, Byeong Hwa
    • The Korean Journal of Physiology and Pharmacology
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    • v.21 no.4
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    • pp.377-384
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    • 2017
  • Activation of protein kinase C (PKC) is closely linked with endothelial dysfunction. However, the effect of $PKC{\beta}II$ on endothelial dysfunction has not been characterized in cultured endothelial cells. Here, using adenoviral $PKC{\beta}II$ gene transfer and pharmacological inhibitors, the role of $PKC{\beta}II$ on endothelial dysfucntion was investigated in cultured endothelial cells. Phorbol 12-myristate 13-acetate (PMA) increased reactive oxygen species (ROS), p66shc phosphorylation, intracellular adhesion molecule-1, and monocyte adhesion, which were inhibited by $PKC{\beta}i$ (10 nM), a selective inhibitor of $PKC{\beta}II$. PMA increased the phosphorylation of CREB and manganese superoxide dismutase (MnSOD), which were also inhibited by $PKC{\beta}i$. Gene silencing of CREB inhibited PMA-induced MnSOD expression, suggesting that CREB plays a key role in MnSOD expression. Gene silencing of $PKC{\beta}II$ inhibited PMA-induced mitochondrial ROS, MnSOD, and ICAM-1 expression. In contrast, overexpression of $PKC{\beta}II$ using adenoviral $PKC{\beta}II$ increased mitochondrial ROS, MnSOD, ICAM-1, and p66shc phosphorylation in cultured endothelial cells. Finally, $PKC{\beta}II$-induced ICAM-1 expression was inhibited by Mito-TEMPO, a mitochondrial ROS scavenger, suggesting the involvement of mitochondrial ROS in PKC-induced vascular inflammation. Taken together, the results suggest that $PKC{\beta}II$ plays an important role in PMA-induced endothelial dysfunction, and that the inhibition of $PKC{\beta}II$-dependent p66shc signaling acts as a therapeutic target for vascular inflammatory diseases.